(Circular) (Six-base) MAPSORT of: pFN21AE6236.seq Check: 7335 from: 1 to: 5422 pFN21AE6236 5422 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2638 2638 Size: 5422 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3232 278 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2765 1937 Size: 828 4594 Acc65I G'GTAC_C Cuts at: 2743 2743 Size: 5422 AclI AA'CG_TT Cuts at: 1563 3549 3922 1563 Size: 1986 373 3063 Fragments arranged by size: 3063 1986 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3485 4869 5137 1632 Size: 1853 1384 268 1917 Fragments arranged by size: 1917 1853 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5225 AflIII A'CryG_T Cuts at: 1203 4321 5298 1203 Size: 3118 977 1327 Fragments arranged by size: 3118 1327 977 AgeI A'CCGG_T Cuts at: 4253 4253 Size: 5422 AhdI GACnn_n'nnGTC Cuts at: 4151 4151 Size: 5422 AleI CACnn'nnGTG Cuts at: 1166 2018 2132 1166 Size: 852 114 4456 Fragments arranged by size: 4456 852 114 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2036 1184 Size: 852 4570 AlwNI CAG_nnn'CTG Cuts at: 2029 2494 3165 4737 2029 Size: 465 671 1572 2714 Fragments arranged by size: 2714 1572 671 465 ApaI G_GGCC'C Cuts at: 2671 2671 Size: 5422 ApaLI G'TGCA_C Cuts at: 2314 3479 4635 2314 Size: 1165 1156 3101 Fragments arranged by size: 3101 1165 1156 ApoI r'AATT_y Cuts at: 1493 2731 2957 3109 5218 1493 Size: 1238 226 152 2109 1697 Fragments arranged by size: 2109 1697 1238 226 152 AseI AT'TA_AT Cuts at: 160 1032 3976 160 Size: 872 2944 1606 Fragments arranged by size: 2944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 AvaI C'yCGr_G Cuts at: 1870 1943 2275 2747 1870 Size: 73 332 472 4545 Fragments arranged by size: 4545 472 332 73 BamHI G'GATC_C Cuts at: 2752 3119 2752 Size: 367 5055 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2114 2369 2537 2569 2743 Size: 325 217 627 327 255 168 32 174 Cuts at: 2743 4198 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 327 325 255 217 174 168 32 BanII G_rGCy'C Cuts at: 1391 2154 2671 2741 1391 Size: 763 517 70 4072 Fragments arranged by size: 4072 763 517 70 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4451 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3613 3647 1903 Size: 34 1676 34 3678 Fragments arranged by size: 3678 1676 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3314 4524 3314 Size: 1210 4212 BclI T'GATC_A Cuts at: 1548 2070 1548 Size: 522 4900 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2015 2092 4033 Size: 107 122 71 631 605 343 77 1941 Cuts at: 4033 136 Size: 1525 Fragments arranged by size: 1941 1525 631 605 343 122 107 77 71 BglII A'GATC_T Cuts at: 2199 5417 2199 Size: 3218 2204 BlpI GC'TnA_GC Cuts at: 2042 2839 2042 Size: 797 4625 Bme1580I G_kGCm'C Cuts at: 1790 2066 2117 2318 2336 2671 3483 4639 1790 Size: 276 51 201 18 335 812 1156 2573 Fragments arranged by size: 2573 1156 812 335 276 201 51 18 BmgBI CAC'GTC Cuts at: 1318 2431 1318 Size: 1113 4309 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3153 4111 442 Size: 416 53 83 399 24 1736 958 1753 Fragments arranged by size: 1753 1736 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5422 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2219 4082 1239 Size: 980 1863 2579 Fragments arranged by size: 2579 1863 980 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3547 4412 4710 3547 Size: 865 298 4259 Fragments arranged by size: 4259 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4085 882 Size: 617 2586 2219 Fragments arranged by size: 2586 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2110 2719 3213 493 Size: 711 906 609 494 2702 Fragments arranged by size: 2702 906 711 609 494 BsaBI GATnn'nnATC Cuts at: 5416 5416 Size: 5422 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3229 3611 4246 275 Size: 53 83 186 936 1696 382 635 1451 Fragments arranged by size: 1696 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3855 4253 4527 4674 5085 3855 Size: 398 274 147 411 4192 Fragments arranged by size: 4192 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2153 2183 1178 Size: 30 165 30 750 30 4417 Fragments arranged by size: 4417 750 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2503 1186 Size: 1317 4105 BseYI C'CCAG_C Cuts at: 2462 4625 5121 2462 Size: 2163 496 2763 Fragments arranged by size: 2763 2163 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2042 2456 1163 Size: 573 306 414 4129 Fragments arranged by size: 4129 573 414 306 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2133 2872 3633 4661 1540 Size: 275 186 18 114 739 761 1028 2301 Fragments arranged by size: 2301 1028 761 739 275 186 114 18 BsiHKAI G_wGCw'C Cuts at: 2318 3483 3568 4639 2318 Size: 1165 85 1071 3101 Fragments arranged by size: 3101 1165 1071 85 BsmI GAATG_Cn' Cuts at: 2933 3026 2933 Size: 93 5329 BsmBI CGTCTCn'nnnn_ Cuts at: 2416 2416 Size: 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2117 2154 2318 2336 2671 2741 Size: 399 276 51 37 164 18 335 70 Cuts at: 2741 3483 3568 4639 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 335 276 164 85 70 51 37 18 BspEI T'CCGG_A Cuts at: 5085 5085 Size: 5422 BspHI T'CATG_A Cuts at: 3311 5041 3311 Size: 1730 3692 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2638 2777 844 Size: 1794 139 3489 Fragments arranged by size: 3489 1794 139 BsrBI CCG'CTC Cuts at: 1394 1823 3309 1394 Size: 429 1486 3507 Fragments arranged by size: 3507 1486 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3917 4091 65 Size: 1183 2669 174 1396 Fragments arranged by size: 2669 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2387 4066 4253 1700 Size: 110 577 1679 187 2869 Fragments arranged by size: 2869 1679 577 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5422 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5422 BssSI C'ACGA_G Cuts at: 3482 4494 3482 Size: 1012 4410 BstAPI GCAn_nnn'nTGC Cuts at: 794 2286 2322 794 Size: 1492 36 3894 Fragments arranged by size: 3894 1492 36 BstBI TT'CG_AA Cuts at: 4226 4226 Size: 5422 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5422 BstXI CCAn_nnnn'nTGG Cuts at: 2431 2431 Size: 5422 BstYI r'GATC_y Cuts at: 1972 2199 2752 3119 3504 3521 4973 5059 5071 Size: 227 553 367 385 17 1452 86 12 Cuts at: 5071 5417 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 553 385 367 346 227 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4683 Fragments arranged by size: 4683 683 56 BtsI GCAGTG_nn' Cuts at: 2613 2938 3732 3752 2613 Size: 325 794 20 4283 Fragments arranged by size: 4283 794 325 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5422 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5387 DraI TTT'AAA Cuts at: 2726 2726 Size: 5422 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4429 809 Size: 507 3113 1802 Fragments arranged by size: 3113 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2016 2130 Size: 54 1425 50 275 18 177 9 114 Cuts at: 2130 2385 2406 2485 2685 2869 3758 5387 8 Size: 255 21 79 200 184 889 1629 43 Fragments arranged by size: 1629 1425 889 275 255 200 184 177 114 79 54 50 43 21 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2016 2130 2869 1537 Size: 275 204 114 739 4090 Fragments arranged by size: 4090 739 275 204 114 EarI CTCTTCn'nnn_ Cuts at: 1353 2381 3352 1353 Size: 1028 971 3423 Fragments arranged by size: 3423 1028 971 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2110 4009 4383 4529 387 Size: 1064 138 521 1899 374 146 1280 Fragments arranged by size: 1899 1280 1064 521 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2219 3485 4082 4869 5137 1239 Size: 393 587 1266 597 787 268 1524 Fragments arranged by size: 1524 1266 787 597 587 393 268 EcoO109I rG'GnC_Cy Cuts at: 2491 2580 2590 2668 2491 Size: 89 10 78 5245 Fragments arranged by size: 5245 89 78 10 EcoRI G'AATT_C Cuts at: 2731 3109 2731 Size: 378 5044 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2368 2368 Size: 5422 FseI GG_CCGG'CC Cuts at: 2391 2391 Size: 5422 FspI TGC'GCA Cuts at: 3928 3928 Size: 5422 HaeII r_GCGC'y Cuts at: 1678 2222 4569 1678 Size: 544 2347 2531 Fragments arranged by size: 2531 2347 544 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4063 4095 4137 4169 1373 Size: 32 135 32 2491 32 42 32 2626 Fragments arranged by size: 2626 2491 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2766 3012 669 Size: 988 281 828 246 3079 Fragments arranged by size: 3079 988 828 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5422 HpaI GTT'AAC Cuts at: 3012 3012 Size: 5422 KpnI G_GTAC'C Cuts at: 2747 2747 Size: 5422 MfeI C'AATT_G Cuts at: 3021 3021 Size: 5422 MluI A'CGCG_T Cuts at: 5298 5298 Size: 5422 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4536 4720 1305 Size: 3231 184 2007 Fragments arranged by size: 3231 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2487 10 Size: 54 1425 343 177 478 2945 Fragments arranged by size: 2945 1425 478 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2018 2132 2306 3380 3739 3898 518 Size: 648 852 114 174 1074 359 159 2042 Fragments arranged by size: 2042 1074 852 648 359 174 159 114 MspA1I CmG'CkG Cuts at: 1606 2455 2466 2648 2697 2838 3515 4663 4908 Size: 849 11 182 49 141 677 1148 245 Cuts at: 4908 1606 Size: 2120 Fragments arranged by size: 2120 1148 849 677 245 182 141 49 11 NaeI GCC'GGC Cuts at: 2389 2389 Size: 5422 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3931 Fragments arranged by size: 3931 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5422 NgoMIV G'CCGG_C Cuts at: 2387 2387 Size: 5422 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5422 NotI GC'GGCC_GC Cuts at: 2869 2869 Size: 5422 NspI r_CATG'y Cuts at: 1132 1229 2780 4325 1132 Size: 97 1551 1545 2229 Fragments arranged by size: 2229 1551 1545 97 PasI CC'CwG_GG Cuts at: 1348 2598 1348 Size: 1250 4172 PciI A'CATG_T Cuts at: 4321 4321 Size: 5422 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5422 PfoI T'CCnGG_A Cuts at: 2414 2414 Size: 5422 PmeI GTTT'AAAC Cuts at: 2726 2726 Size: 5422 PmlI CAC'GTG Cuts at: 2110 2110 Size: 5422 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3491 3523 5004 5036 3491 Size: 32 1481 32 3877 Fragments arranged by size: 3877 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5422 PsiI TTA'TAA Cuts at: 2992 2992 Size: 5422 PspOMI G'GGCC_C Cuts at: 2667 2667 Size: 5422 PstI C_TGCA'G Cuts at: 830 2774 830 Size: 1944 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5422 PvuII CAG'CTG Cuts at: 2648 2648 Size: 5422 SalI G'TCGA_C Cuts at: 1936 2764 1936 Size: 828 4594 SbfI CC_TGCA'GG Cuts at: 2774 2774 Size: 5422 ScaI AGT'ACT Cuts at: 3670 5382 3670 Size: 1712 3710 SfcI C'TryA_G Cuts at: 826 1045 2575 2770 3905 4586 4777 826 Size: 219 1530 195 1135 681 191 1471 Fragments arranged by size: 1530 1471 1135 681 219 195 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2015 2015 Size: 5422 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5422 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5422 SmaI CCC'GGG Cuts at: 1872 2749 1872 Size: 877 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3526 4427 4689 820 Size: 197 926 1583 901 262 1553 Fragments arranged by size: 1583 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3213 493 Size: 2720 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5422 SphI G_CATG'C Cuts at: 1132 2780 1132 Size: 1648 3774 SspI AAT'ATT Cuts at: 5 52 3346 5 Size: 47 3294 2081 Fragments arranged by size: 3294 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5422 StyI C'CwwG_G Cuts at: 513 1065 2004 2328 2861 513 Size: 552 939 324 533 3074 Fragments arranged by size: 3074 939 552 533 324 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3449 3466 3619 3804 4249 325 Size: 1255 1869 17 153 185 445 1498 Fragments arranged by size: 1869 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3668 5380 96 Size: 274 80 33 51 1443 1691 1712 138 Fragments arranged by size: 1712 1691 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2149 2153 2181 2185 5382 5414 2149 Size: 4 28 4 3197 32 2157 Fragments arranged by size: 3197 2157 32 28 4 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5422 XbaI T'CTAG_A Cuts at: 2758 2758 Size: 5422 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2428 Size: 5422 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5422 XmaI C'CCGG_G Cuts at: 1870 2747 1870 Size: 877 4545 XmnI GAAnn'nnTTC Cuts at: 3551 3551 Size: 5422 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3230 276 Size: 53 83 186 936 1696 2468 Fragments arranged by size: 2468 1696 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FspAI KasI NarI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI