################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## Acc65I AccIII AclI AcsI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BbeI BbsI BcgI BciVI BclI BfaI BfrI BglII BlnI BpuAI BsaI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tsp509I Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 601 GACGTC GACGT^C AccI 604 GTCTAC GT^[AC][GT]AC AccII 163 CGCG CG^CG AluI 353 AGCT AG^CT AlwNI 562 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG BamHI 23 GGATCC G^GATCC BbrPI 616 CACGTG CAC^GTG BbvCI 88 GCTGAGG CCTCAGC(-5/-2) BbvI 369 GCAGC GCAGC(8/12) BglI 249 GCCGCTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 72 GCTGAGC GC^T[ACGT]AGC Bpu1102I 72 GCTGAGC GC^T[ACGT]AGC BsaAI 616 CACGTG [CT]AC^GT[GA] BsaBI 457 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 482 TCCGGT [AT]^CCGG[AT] BsmBI 599 GAGACG CGTCTC(1/5) BsmFI 154 GGGAC GGGAC(10/14) BstF5I 404 CATCC GGATG(2/0) BstUI 163 CGCG CG^CG BstXI 644 CCATCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 72 GCTGAGC GC^T[ACGT]AGC CfoI 162 GCGC GCG^C DraIII 324 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG FauI 660 GCGGG CCCGC(4/6) FokI 404 CATCC GGATG(9/13) HhaI 162 GCGC GCG^C HinP1I 162 GCGC G^CGC MamI 457 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 128 TTAA T^TAA MvnI 163 CGCG CG^CG NlaIII 135 CATG CATG^ NspI 134 GCATGT [GA]CATG^[CT] PflMI 561 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 616 CACGTG CAC^GTG PmlI 616 CACGTG CAC^GTG PpuMI 304 AGGACCC [GA]G^G[AT]CC[CT] SacI 352 GAGCTC GAGCT^C ScaI 272 AGTACT AGT^ACT SmaI 308 CCCGGG CCC^GGG StuI 33 AGGCCT AGG^CCT TaqI 215 TCGA T^CGA TfiI 673 GAATC G^A[AT]TC Tru9I 128 TTAA T^TAA TseI 369 GCAGC G^C[AT]GC TthHB8I 215 TCGA T^CGA Van91I 561 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 308 CCCGGG C^CCGGG XmaI 308 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AcyI 246 GACGCC G[GA]^CG[CT]C 601 GACGTC AvaII 305 GGACC G^G[AT]CC 554 GGACC BsaHI 246 GACGCC G[GA]^CG[CT]C 601 GACGTC BseRI 199 GAGGAG GAGGAG(10/8) 505 GAGGAG BsgI 778 CTGCAC GTGCAG(16/14) 330 GTGCAG BspMI 434 ACCTGC ACCTGC(4/8) 610 ACCTGC BstYI 23 GGATCC [GA]^GATC[CT] 563 AGATCC DraII 304 AGGACCC [GA]G^G[ACGT]CC[CT] 416 AGGCCCT EcoO109I 304 AGGACCC [GA]G^G[ACGT]CC[CT] 416 AGGCCCT HgaI 246 GACGC GACGC(5/10) 168 GCGTC Hin1I 246 GACGCC G[GA]^CG[CT]C 601 GACGTC MboII 9 GAAGA GAAGA(8/7) 728 TCTTC MflI 23 GGATCC [GA]^GATC[CT] 563 AGATCC MslI 78 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 645 CATCATCGTG MspA1I 250 CCGCTG C[AC]G^C[GT]G 370 CAGCGG TspRI 389 CAGTG CA[GC]TG[ACGT][ACGT]^ 449 CAGTG XhoII 23 GGATCC [GA]^GATC[CT] 563 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AlwI AspHI AvaI BanII BcnI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstNI Cac8I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeII MaeIII MboI MnlI MspI MvaI MwoI NciI NdeII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI Tsp45I