(Circular) (Six-base) MAPSORT of: pFN21AE1548.seq Check: 3981 from: 1 to: 5488 pFN21AE1548 5488 bp With 182 enzymes: SgfI * August 16, 2010 14:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2624 2624 Size: 5488 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2610 3298 278 Size: 53 83 186 936 1074 688 2468 Fragments arranged by size: 2468 1074 936 688 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 2831 1937 Size: 673 221 4594 Fragments arranged by size: 4594 673 221 Acc65I G'GTAC_C Cuts at: 2809 2809 Size: 5488 AclI AA'CG_TT Cuts at: 1563 3615 3988 1563 Size: 2052 373 3063 Fragments arranged by size: 3063 2052 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3551 4935 5203 1632 Size: 1919 1384 268 1917 Fragments arranged by size: 1919 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5291 AflIII A'CryG_T Cuts at: 1203 4387 5364 1203 Size: 3184 977 1327 Fragments arranged by size: 3184 1327 977 AgeI A'CCGG_T Cuts at: 4319 4319 Size: 5488 AhdI GACnn_n'nnGTC Cuts at: 4217 4217 Size: 5488 AleI CACnn'nnGTG Cuts at: 1166 2087 1166 Size: 921 4567 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2387 1184 Size: 1203 4285 AlwNI CAG_nnn'CTG Cuts at: 2572 3231 4803 2572 Size: 659 1572 3257 Fragments arranged by size: 3257 1572 659 ApaLI G'TGCA_C Cuts at: 3545 4701 3545 Size: 1156 4332 ApoI r'AATT_y Cuts at: 1493 2797 3023 3175 5284 1493 Size: 1304 226 152 2109 1697 Fragments arranged by size: 2109 1697 1304 226 152 AseI AT'TA_AT Cuts at: 160 1032 4042 160 Size: 872 3010 1606 Fragments arranged by size: 3010 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2313 2738 2813 1870 Size: 73 168 202 425 75 4545 Fragments arranged by size: 4545 425 202 168 75 73 BamHI G'GATC_C Cuts at: 2028 2818 3185 2028 Size: 790 367 4331 Fragments arranged by size: 4331 790 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2809 4264 618 Size: 325 217 627 1022 1455 1842 Fragments arranged by size: 1842 1455 1022 627 325 217 BanII G_rGCy'C Cuts at: 1391 2361 2391 2671 2781 2807 1391 Size: 970 30 280 110 26 4072 Fragments arranged by size: 4072 970 280 110 30 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4517 BbvCI CC'TCA_GC Cuts at: 2094 2094 Size: 5488 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3679 3713 1903 Size: 34 1742 34 3678 Fragments arranged by size: 3678 1742 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3380 4590 3380 Size: 1210 4278 BclI T'GATC_A Cuts at: 1548 1548 Size: 5488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2260 4099 136 Size: 107 122 71 631 605 588 1839 1525 Fragments arranged by size: 1839 1525 631 605 588 122 107 71 BglII A'GATC_T Cuts at: 5483 5483 Size: 5488 BlpI GC'TnA_GC Cuts at: 2078 2905 2078 Size: 827 4661 Bme1580I G_kGCm'C Cuts at: 1790 3549 4705 1790 Size: 1759 1156 2573 Fragments arranged by size: 2573 1759 1156 BmgBI CAC'GTC Cuts at: 1318 2230 1318 Size: 912 4576 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3219 4177 442 Size: 416 53 83 399 24 1802 958 1753 Fragments arranged by size: 1802 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5488 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2055 2324 2624 4148 1239 Size: 816 269 300 1524 2579 Fragments arranged by size: 2579 1524 816 300 269 Bpu10I CC'TnA_GC Cuts at: 2094 2094 Size: 5488 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3613 4478 4776 3613 Size: 865 298 4325 Fragments arranged by size: 4325 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4151 882 Size: 617 2652 2219 Fragments arranged by size: 2652 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2623 3279 493 Size: 711 1419 656 2702 Fragments arranged by size: 2702 1419 711 656 BsaBI GATnn'nnATC Cuts at: 2466 5482 2466 Size: 3016 2472 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2252 2607 3295 3677 Size: 53 83 186 936 719 355 688 382 Cuts at: 3677 4312 275 Size: 635 1451 Fragments arranged by size: 1451 936 719 688 635 382 355 186 83 53 BsaWI w'CCGG_w Cuts at: 2487 3921 4319 4593 4740 5151 2487 Size: 1434 398 274 147 411 2824 Fragments arranged by size: 2824 1434 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2703 2733 1178 Size: 30 165 30 1300 30 3933 Fragments arranged by size: 3933 1300 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2219 2525 1186 Size: 1033 306 4149 Fragments arranged by size: 4149 1033 306 BseYI C'CCAG_C Cuts at: 2257 2595 2633 2722 2743 4691 5187 2257 Size: 338 38 89 21 1948 496 2558 Fragments arranged by size: 2558 1948 496 338 89 38 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2356 2768 1163 Size: 573 620 412 3883 Fragments arranged by size: 3883 620 573 412 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2938 3699 4727 1540 Size: 275 186 937 761 1028 2301 Fragments arranged by size: 2301 1028 937 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 2361 2631 3549 3634 4705 2084 Size: 277 270 918 85 1071 2867 Fragments arranged by size: 2867 1071 918 277 270 85 BsmI GAATG_Cn' Cuts at: 2999 3092 2999 Size: 93 5395 BsmBI CGTCTCn'nnnn_ Cuts at: 2598 2598 Size: 5488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2084 2361 2391 2631 2671 2781 2807 Size: 399 294 277 30 240 40 110 26 Cuts at: 2807 3549 3634 4705 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 294 277 240 110 85 40 30 26 BspEI T'CCGG_A Cuts at: 5151 5151 Size: 5488 BspHI T'CATG_A Cuts at: 3377 5107 3377 Size: 1730 3758 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2448 2624 2843 844 Size: 1604 176 219 3489 Fragments arranged by size: 3489 1604 219 176 BsrBI CCG'CTC Cuts at: 1394 1823 3375 1394 Size: 429 1552 3507 Fragments arranged by size: 3507 1552 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3983 4157 65 Size: 1183 2735 174 1396 Fragments arranged by size: 2735 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4132 4319 1700 Size: 110 2322 187 2869 Fragments arranged by size: 2869 2322 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5488 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5488 BssSI C'ACGA_G Cuts at: 3548 4560 3548 Size: 1012 4476 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5488 BstBI TT'CG_AA Cuts at: 4292 4292 Size: 5488 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5488 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 5488 BstYI r'GATC_y Cuts at: 1972 2028 2568 2818 3185 3570 3587 5039 5125 Size: 56 540 250 367 385 17 1452 86 Cuts at: 5125 5137 5483 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 540 385 367 346 250 86 56 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4936 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2087 487 Size: 683 56 861 3888 Fragments arranged by size: 3888 861 683 56 BtsI GCAGTG_nn' Cuts at: 3004 3798 3818 3004 Size: 794 20 4674 Fragments arranged by size: 4674 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5488 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5453 DraI TTT'AAA Cuts at: 2792 2792 Size: 5488 DraIII CAC_nnn'GTG Cuts at: 2334 2334 Size: 5488 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4495 809 Size: 507 3179 1802 Fragments arranged by size: 3179 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2935 3824 5453 Size: 54 1425 50 275 18 1105 889 1629 Cuts at: 5453 8 Size: 43 Fragments arranged by size: 1629 1425 1105 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2935 1537 Size: 275 1123 4090 Fragments arranged by size: 4090 1123 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3418 1353 Size: 2065 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2333 4075 4449 4595 387 Size: 1064 138 744 1742 374 146 1280 Fragments arranged by size: 1742 1280 1064 744 374 146 138 EcoICRI GAG'CTC Cuts at: 2359 2359 Size: 5488 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2055 2324 2624 3551 4148 4935 5203 Size: 393 423 269 300 927 597 787 268 Cuts at: 5203 1239 Size: 1524 Fragments arranged by size: 1524 927 787 597 423 393 300 269 268 EcoO109I rG'GnC_Cy Cuts at: 2310 2422 2310 Size: 112 5376 EcoRI G'AATT_C Cuts at: 2797 3175 2797 Size: 378 5110 FspI TGC'GCA Cuts at: 3994 3994 Size: 5488 HaeII r_GCGC'y Cuts at: 1678 4635 1678 Size: 2957 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4129 4161 4203 4235 1373 Size: 32 135 32 2557 32 42 32 2626 Fragments arranged by size: 2626 2557 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2832 3078 669 Size: 988 281 894 246 3079 Fragments arranged by size: 3079 988 894 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5488 HpaI GTT'AAC Cuts at: 3078 3078 Size: 5488 KpnI G_GTAC'C Cuts at: 2813 2813 Size: 5488 MfeI C'AATT_G Cuts at: 3087 3087 Size: 5488 MluI A'CGCG_T Cuts at: 5364 5364 Size: 5488 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2304 2334 4602 4786 1305 Size: 999 30 2268 184 2007 Fragments arranged by size: 2268 2007 999 184 30 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3666 Fragments arranged by size: 3666 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2654 3446 3805 3964 518 Size: 648 921 567 792 359 159 2042 Fragments arranged by size: 2042 921 792 648 567 359 159 MspA1I CmG'CkG Cuts at: 1606 2257 2377 2904 3581 4729 4974 1606 Size: 651 120 527 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 651 527 245 120 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4936 NdeI CA'TA_TG Cuts at: 387 387 Size: 5488 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5488 NotI GC'GGCC_GC Cuts at: 2935 2935 Size: 5488 NspI r_CATG'y Cuts at: 1132 1229 2143 2846 4391 1132 Size: 97 914 703 1545 2229 Fragments arranged by size: 2229 1545 914 703 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5488 PciI A'CATG_T Cuts at: 4387 4387 Size: 5488 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2572 1108 Size: 902 562 4024 Fragments arranged by size: 4024 902 562 PfoI T'CCnGG_A Cuts at: 2301 2411 2301 Size: 110 5378 PmeI GTTT'AAAC Cuts at: 2792 2792 Size: 5488 PmlI CAC'GTG Cuts at: 2623 2623 Size: 5488 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3557 3589 5070 5102 3557 Size: 32 1481 32 3943 Fragments arranged by size: 3943 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2310 2310 Size: 5488 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5488 PsiI TTA'TAA Cuts at: 3058 3058 Size: 5488 PstI C_TGCA'G Cuts at: 830 2840 830 Size: 2010 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5488 SacI G_AGCT'C Cuts at: 2361 2361 Size: 5488 SalI G'TCGA_C Cuts at: 1936 2830 1936 Size: 894 4594 SbfI CC_TGCA'GG Cuts at: 2840 2840 Size: 5488 ScaI AGT'ACT Cuts at: 2279 3736 5448 2279 Size: 1457 1712 2319 Fragments arranged by size: 2319 1712 1457 SfcI C'TryA_G Cuts at: 826 1045 2836 3971 4652 4843 826 Size: 219 1791 1135 681 191 1471 Fragments arranged by size: 1791 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5488 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5488 SmaI CCC'GGG Cuts at: 1872 2315 2815 1872 Size: 443 500 4545 Fragments arranged by size: 4545 500 443 SmlI C'TyrA_G Cuts at: 820 1017 1943 3592 4493 4755 820 Size: 197 926 1649 901 262 1553 Fragments arranged by size: 1649 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3279 493 Size: 2786 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5488 SphI G_CATG'C Cuts at: 1132 2846 1132 Size: 1714 3774 SspI AAT'ATT Cuts at: 5 52 3412 5 Size: 47 3360 2081 Fragments arranged by size: 3360 2081 47 StuI AGG'CCT Cuts at: 1517 2040 1517 Size: 523 4965 StyI C'CwwG_G Cuts at: 513 1065 2927 513 Size: 552 1862 3074 Fragments arranged by size: 3074 1862 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3515 3532 3685 3870 4315 325 Size: 1255 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 3734 5446 Size: 274 80 33 51 1443 300 1457 1712 Cuts at: 5446 96 Size: 138 Fragments arranged by size: 1712 1457 1443 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2318 2350 2703 2735 5448 5480 2318 Size: 32 353 32 2713 32 2326 Fragments arranged by size: 2713 2326 353 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5488 XbaI T'CTAG_A Cuts at: 2824 2824 Size: 5488 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5488 XmaI C'CCGG_G Cuts at: 1870 2313 2813 1870 Size: 443 500 4545 Fragments arranged by size: 4545 500 443 XmnI GAAnn'nnTTC Cuts at: 3617 3617 Size: 5488 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2608 3296 276 Size: 53 83 186 936 1074 688 2468 Fragments arranged by size: 2468 1074 936 688 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI