Restriction map for
pFN21AB7913
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# NO CUT ENZYMES ( 172 enzymes) #
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AatII Acc65I AccI AclI AcsI
AcyI AflII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AvaII AviII AvrII BanI BanII
BbeI BbrPI BbsI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI Hin1I HincII HindII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeII MamI MfeI MluI
MseI MslI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tru9I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 308 CGCG CG^CG
AccIII 464 TCCGGA T^CCGGA
AciI 309 GCGG CCGC(-3/-1)
AfaI 101 GTAC GT^AC
AflIII 374 ACATGT A^C[GA][CT]GT
BalI 92 TGGCCA TGG^CCA
BbvCI 17 CCTCAGC CCTCAGC(-5/-2)
BfaI 323 CTAG C^TAG
BpmI 331 CTGGAG CTGGAG(16/14)
BseAI 464 TCCGGA T^CCGGA
BseRI 241 GAGGAG GAGGAG(10/8)
BspEI 464 TCCGGA T^CCGGA
BspLU11I 374 ACATGT A^CATGT
BstUI 308 CGCG CG^CG
Cfr13I 13 GGCCC G^G[ACGT]CC
DsaI 89 CCATGG C^C[GA][CT]GG
EaeI 92 TGGCCA [CT]^GGCC[GA]
EcoT14I 89 CCATGG C^C[AT][AT]GG
HaeII 362 AGCGCC [GA]GCGC^[CT]
HindIII 422 AAGCTT A^AGCTT
MaeI 323 CTAG C^TAG
MaeIII 286 GTGAC ^GT[ACGT]AC
MluNI 92 TGGCCA TGG^CCA
MroI 464 TCCGGA T^CCGGA
MscI 92 TGGCCA TGG^CCA
MspA1I 349 CAGCTG C[AC]G^C[GT]G
MvnI 308 CGCG CG^CG
NcoI 89 CCATGG C^CATGG
NspI 374 ACATGT [GA]CATG^[CT]
PflMI 385 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PvuII 349 CAGCTG CAG^CTG
RsaI 101 GTAC GT^AC
Sau96I 13 GGCCC G^G[ACGT]CC
ScaI 100 AGTACT AGT^ACT
StyI 89 CCATGG C^C[AT][AT]GG
TaqI 275 TCGA T^CGA
Tsp45I 286 GTGAC ^GT[GC]AC
TthHB8I 275 TCGA T^CGA
Van91I 385 CCAGGATTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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BamHI 210 GGATCC G^GATCC
380 GGATCC
BsaWI 57 ACCGGA [AT]^CCGG[AT]
464 TCCGGA
BsrI 6 CCAGT ACTGG(1/-1)
366 CCAGT
BstF5I 47 CATCC GGATG(2/0)
228 GGATG
BstYI 210 GGATCC [GA]^GATC[CT]
380 GGATCC
CfoI 307 GCGC GCG^C
363 GCGC
EcoNI 76 CCTCCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
130 CCTGGGGCAGG
FokI 47 CATCC GGATG(9/13)
228 GGATG
HaeIII 13 GGCC GG^CC
93 GGCC
HapII 58 CCGG C^CGG
465 CCGG
HhaI 307 GCGC GCG^C
363 GCGC
HinP1I 307 GCGC G^CGC
363 GCGC
HpaII 58 CCGG C^CGG
465 CCGG
HphI 285 GGTGA GGTGA(8/7)
336 GGTGA
MflI 210 GGATCC [GA]^GATC[CT]
380 GGATCC
MspI 58 CCGG C^CGG
465 CCGG
NlaIII 90 CATG CATG^
375 CATG
PstI 104 CTGCAG CTGCA^G
253 CTGCAG
SfaNI 246 GATGC GCATC(5/9)
312 GCATC
SfcI 104 CTGCAG C^T[GA][CT]AG
253 CTGCAG
Tsp509I 40 AATT ^AATT
185 AATT
TspRI 107 CAGTG CA[GC]TG[ACGT][ACGT]^
367 CAGTG
XhoII 210 GGATCC [GA]^GATC[CT]
380 GGATCC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwI BbvI BsaJI BsiYI
BslI BssKI BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI ItaI MboI
MboII MnlI MvaI MwoI NdeII
NlaIV Sau3AI ScrFI TseI