################################## # NO CUT ENZYMES ( 86 enzymes) # ################################## AccI AccIII AclI AflII AflIII AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AspI AviII BalI BamHI BcgI BciVI BfrI BglI BsaBI BseAI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FspI HincII HindII HindIII HpaI KspI MamI MluI MluNI MroI MscI NdeI NheI NotI NruI NsiI NspV PacI PflFI PflMI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI ScaI SexAI SfiI SfuI SmaI SnaBI SpeI SspI SwaI Tth111I Van91I XbaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AatII 961 GACGTC GACGT^C AccII 1265 CGCG CG^CG AcsI 632 AAATTC [GA]^AATT[CT] AgeI 6 ACCGGT A^CCGGT Alw44I 1858 GTGCAC G^TGCAC ApaLI 1858 GTGCAC G^TGCAC ApoI 632 AAATTC [GA]^AATT[CT] AvrII 1773 CCTAGG C^CTAGG BbeI 1271 GGCGCC GGCGC^C BbrPI 526 CACGTG CAC^GTG BclI 1386 TGATCA T^GATCA BglII 1464 AGATCT A^GATCT BlnI 1773 CCTAGG C^CTAGG BsaAI 526 CACGTG [CT]AC^GT[GA] BsiEI 1207 CGGCCG CG[GA][CT]^CG BsmBI 719 GAGACG CGTCTC(1/5) Bsp1407I 1427 TGTACA T^GTACA BsrGI 1427 TGTACA T^GTACA BstEII 1933 GGTGACC G^GT[ACGT]ACC BstPI 1933 GGTGACC G^GT[ACGT]ACC BstUI 1265 CGCG CG^CG BstXI 525 CCACGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 721 GACGAGGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 1207 CGGCCG [CT]^GGCC[GA] EagI 1207 CGGCCG C^GGCCG EclXI 1207 CGGCCG C^GGCCG Eco52I 1207 CGGCCG C^GGCCG EcoO65I 1933 GGTGACC G^GT[ACGT]ACC FbaI 1386 TGATCA T^GATCA FseI 1204 GGCCGGCC GGCCGG^CC KasI 1271 GGCGCC G^GCGCC MfeI 648 CAATTG C^AATTG MseI 409 TTAA T^TAA MunI 648 CAATTG C^AATTG MvnI 1265 CGCG CG^CG NarI 1271 GGCGCC GG^CGCC NcoI 1641 CCATGG C^CATGG PinAI 6 ACCGGT A^CCGGT PmaCI 526 CACGTG CAC^GTG PmlI 526 CACGTG CAC^GTG PshAI 1123 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SapI 2101 GAAGAGC GCTCTTC(1/4) SbfI 66 CCTGCAGG CCTGCA^GG SfaNI 1499 GCATC GCATC(5/9) SfoI 1271 GGCGCC GGC^GCC SgrAI 1177 CGCCGGCG C[GA]^CCGG[CT]G SphI 424 GCATGC GCATG^C Sse8387I 66 CCTGCAGG CCTGCA^GG SspBI 1427 TGTACA T^GTACA StuI 1155 AGGCCT AGG^CCT TfiI 1114 GAATC G^A[AT]TC Tru9I 409 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### Acc65I 392 GGTACC G^GTACC 1313 GGTACC AocI 510 CCTGAGG CC^T[ACGT]AGG 883 CCTCAGG Asp718I 392 GGTACC G^GTACC 1313 GGTACC BsaWI 6 ACCGGT [AT]^CCGG[AT] 858 ACCGGA BsgI 338 GTGCAG GTGCAG(16/14) 604 GTGCAG BstAPI 1860 GCACCGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1902 GCAGGAGCTGC Bsu36I 510 CCTGAGG CC^T[ACGT]AGG 883 CCTCAGG DraIII 658 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 1861 CACCGTGTG DsaI 1641 CCATGG C^C[GA][CT]GG 1684 CCACGG Eco81I 510 CCTGAGG CC^T[ACGT]AGG 883 CCTCAGG EcoNI 243 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1791 CCTGACCCAGG HaeII 1271 GGCGCC [GA]GCGC^[CT] 1872 GGCGCT KpnI 392 GGTACC GGTAC^C 1313 GGTACC MaeII 527 ACGT A^CGT 962 ACGT MaeIII 1217 GTGAC ^GT[ACGT]AC 1934 GTGAC NaeI 1178 GCCGGC GCC^GGC 1205 GCCGGC NgoMIV 1178 GCCGGC G^CCGGC 1205 GCCGGC NspI 424 GCATGC [GA]CATG^[CT] 1472 GCATGT PaeR7I 136 CTCGAG C^TCGAG 595 CTCGAG TaqI 137 TCGA T^CGA 596 TCGA Tsp45I 1217 GTGAC ^GT[GC]AC 1934 GTGAC Tsp509I 633 AATT ^AATT 649 AATT TthHB8I 137 TCGA T^CGA 596 TCGA XcmI 566 CCAAGGAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1361 CCAACTATAGTCTGG XhoI 136 CTCGAG C^TCGAG 595 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfcI SmlI StyI TseI TspRI XhoII