(Circular) (Six-base) MAPSORT of: pFN21AB5712.seq Check: 8605 from: 1 to: 6865 pFN21AB5712 6865 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2596 2740 3670 2596 Size: 144 930 5791 Fragments arranged by size: 5791 930 144 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2970 4675 278 Size: 53 83 186 936 1434 1705 2468 Fragments arranged by size: 2468 1705 1434 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4208 1937 Size: 2271 4594 Acc65I G'GTAC_C Cuts at: 2397 3318 4186 2397 Size: 921 868 5076 Fragments arranged by size: 5076 921 868 AclI AA'CG_TT Cuts at: 1563 4992 5365 1563 Size: 3429 373 3063 Fragments arranged by size: 3429 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2867 2982 4056 4125 4928 6312 6580 1632 Size: 1235 115 1074 69 803 1384 268 1917 Fragments arranged by size: 1917 1384 1235 1074 803 268 115 69 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6668 AflIII A'CryG_T Cuts at: 1203 5764 6741 1203 Size: 4561 977 1327 Fragments arranged by size: 4561 1327 977 AgeI A'CCGG_T Cuts at: 2011 5696 2011 Size: 3685 3180 AhdI GACnn_n'nnGTC Cuts at: 5594 5594 Size: 6865 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6865 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2132 3500 1184 Size: 948 1368 4549 Fragments arranged by size: 4549 1368 948 AlwNI CAG_nnn'CTG Cuts at: 2212 2495 2896 3029 3796 3913 4608 6180 2212 Size: 283 401 133 767 117 695 1572 2897 Fragments arranged by size: 2897 1572 767 695 401 283 133 117 ApaI G_GGCC'C Cuts at: 2956 3269 3535 2956 Size: 313 266 6286 Fragments arranged by size: 6286 313 266 ApaLI G'TGCA_C Cuts at: 3863 4922 6078 3863 Size: 1059 1156 4650 Fragments arranged by size: 4650 1156 1059 ApoI r'AATT_y Cuts at: 1493 2637 4174 4400 4552 6661 1493 Size: 1144 1537 226 152 2109 1697 Fragments arranged by size: 2109 1697 1537 1144 226 152 AseI AT'TA_AT Cuts at: 160 1032 5419 160 Size: 872 4387 1606 Fragments arranged by size: 4387 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 AvaI C'yCGr_G Cuts at: 1870 1943 2141 2600 3543 4190 1870 Size: 73 198 459 943 647 4545 Fragments arranged by size: 4545 943 647 459 198 73 AvrII C'CTAG_G Cuts at: 3778 3778 Size: 6865 BamHI G'GATC_C Cuts at: 4195 4562 4195 Size: 367 6498 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2397 3247 3276 3318 4186 Size: 325 217 627 610 850 29 42 868 Cuts at: 4186 5641 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 868 850 627 610 325 217 42 29 BanII G_rGCy'C Cuts at: 1391 2181 2219 2921 2956 3051 3114 3269 3535 Size: 790 38 702 35 95 63 155 266 Cuts at: 3535 3821 4184 1391 Size: 286 363 4072 Fragments arranged by size: 4072 790 702 363 286 266 155 95 63 38 35 BbeI G_GCGC'C Cuts at: 3280 3280 Size: 6865 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2970 2979 3558 928 Size: 971 1071 9 579 4235 Fragments arranged by size: 4235 1071 971 579 9 BbvCI CC'TCA_GC Cuts at: 3345 3755 3846 3345 Size: 410 91 6364 Fragments arranged by size: 6364 410 91 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5056 5090 1903 Size: 34 3119 34 3678 Fragments arranged by size: 3678 3119 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4757 5967 4757 Size: 1210 5655 BclI T'GATC_A Cuts at: 1548 3391 1548 Size: 1843 5022 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5476 136 Size: 107 122 71 631 605 3804 1525 Fragments arranged by size: 3804 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3469 6860 3469 Size: 3391 3474 BlpI GC'TnA_GC Cuts at: 2390 2462 3176 4282 2390 Size: 72 714 1106 4973 Fragments arranged by size: 4973 1106 714 72 Bme1580I G_kGCm'C Cuts at: 1790 2510 2956 3269 3535 3867 4926 6082 1790 Size: 720 446 313 266 332 1059 1156 2573 Fragments arranged by size: 2573 1156 1059 720 446 332 313 266 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6865 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2991 4596 5554 Size: 416 53 83 399 24 1574 1605 958 Cuts at: 5554 442 Size: 1753 Fragments arranged by size: 1753 1605 1574 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6865 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4015 4015 Size: 6865 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2105 2189 2203 2742 3090 3507 5525 1239 Size: 866 84 14 539 348 417 2018 2579 Fragments arranged by size: 2579 2018 866 539 417 348 84 14 Bpu10I CC'TnA_GC Cuts at: 2213 3045 3345 3755 3846 3945 2213 Size: 832 300 410 91 99 5133 Fragments arranged by size: 5133 832 410 300 99 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3281 3612 3878 4990 5855 6153 3281 Size: 331 266 1112 865 298 3993 Fragments arranged by size: 3993 1112 865 331 298 266 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2012 2166 2861 3560 3834 5528 882 Size: 617 513 154 695 699 274 1694 2219 Fragments arranged by size: 2219 1694 699 695 617 513 274 154 BsaAI yAC'GTr Cuts at: 493 1204 2533 4656 493 Size: 711 1329 2123 2702 Fragments arranged by size: 2702 2123 1329 711 BsaBI GATnn'nnATC Cuts at: 6859 6859 Size: 6865 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2373 2882 2967 3277 Size: 53 83 186 936 840 509 85 310 Cuts at: 3277 4672 5054 5689 275 Size: 1395 382 635 1451 Fragments arranged by size: 1451 1395 936 840 635 509 382 310 186 85 83 53 BsaWI w'CCGG_w Cuts at: 2011 2863 5298 5696 5970 6117 6528 2011 Size: 852 2435 398 274 147 411 2348 Fragments arranged by size: 2435 2348 852 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2390 2420 2715 2745 3017 Size: 30 165 30 987 30 295 30 272 Cuts at: 3017 3047 1178 Size: 30 4996 Fragments arranged by size: 4996 987 295 272 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2369 2471 2780 2929 1186 Size: 1183 102 309 149 5122 Fragments arranged by size: 5122 1183 309 149 102 BseYI C'CCAG_C Cuts at: 2537 3008 3073 3085 3205 3596 3617 3632 3934 Size: 471 65 12 120 391 21 15 302 Cuts at: 3934 6068 6564 2537 Size: 2134 496 2838 Fragments arranged by size: 2838 2134 496 471 391 302 120 65 21 15 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2364 2630 1163 Size: 573 628 266 5398 Fragments arranged by size: 5398 628 573 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3215 4315 5076 6104 1540 Size: 275 186 1214 1100 761 1028 2301 Fragments arranged by size: 2301 1214 1100 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2181 2219 2601 3297 3821 3867 3890 4113 4926 Size: 38 382 696 524 46 23 223 813 Cuts at: 4926 5011 6082 2181 Size: 85 1071 2964 Fragments arranged by size: 2964 1071 813 696 524 382 223 85 46 38 23 BsmI GAATG_Cn' Cuts at: 4376 4469 4376 Size: 93 6772 BsmBI CGTCTCn'nnnn_ Cuts at: 2718 2718 Size: 6865 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2219 2510 2601 2921 2956 3051 Size: 399 391 38 291 91 320 35 95 Cuts at: 3051 3114 3269 3297 3535 3821 3867 3890 4113 Size: 63 155 28 238 286 46 23 223 Cuts at: 4113 4184 4926 5011 6082 1391 Size: 71 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 391 320 291 286 238 223 155 95 91 85 71 63 46 38 35 28 23 BspEI T'CCGG_A Cuts at: 6528 6528 Size: 6865 BspHI T'CATG_A Cuts at: 4754 6484 4754 Size: 1730 5135 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2124 2247 2385 2596 2740 3509 3670 3907 Size: 1280 123 138 211 144 769 161 237 Cuts at: 3907 4220 844 Size: 313 3489 Fragments arranged by size: 3489 1280 769 313 237 211 161 144 138 123 BsrBI CCG'CTC Cuts at: 1394 1823 4752 1394 Size: 429 2929 3507 Fragments arranged by size: 3507 2929 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5360 5534 65 Size: 1183 4112 174 1396 Fragments arranged by size: 4112 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2011 3183 3210 5509 5696 1700 Size: 110 201 1172 27 2299 187 2869 Fragments arranged by size: 2869 2299 1172 201 187 110 27 BsrGI T'GTAC_A Cuts at: 96 3432 96 Size: 3336 3529 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6865 BssSI C'ACGA_G Cuts at: 4925 5937 4925 Size: 1012 5853 BstAPI GCAn_nnn'nTGC Cuts at: 794 3871 3913 794 Size: 3077 42 3746 Fragments arranged by size: 3746 3077 42 BstBI TT'CG_AA Cuts at: 5669 5669 Size: 6865 BstEII G'GTnAC_C Cuts at: 1184 3938 1184 Size: 2754 4111 BstXI CCAn_nnnn'nTGG Cuts at: 2537 2537 Size: 6865 BstYI r'GATC_y Cuts at: 1972 2439 2520 2541 2712 2853 3469 4195 4562 Size: 467 81 21 171 141 616 726 367 Cuts at: 4562 4947 4964 6416 6502 6514 6860 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 726 616 467 385 367 346 171 141 86 81 21 17 12 Bsu36I CC'TnA_GG Cuts at: 2516 2889 2516 Size: 373 6492 BtgI C'CryG_G Cuts at: 513 1065 2004 3646 3689 513 Size: 552 939 1642 43 3689 Fragments arranged by size: 3689 1642 939 552 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6126 Fragments arranged by size: 6126 683 56 BtsI GCAGTG_nn' Cuts at: 3727 4381 5175 5195 3727 Size: 654 794 20 5397 Fragments arranged by size: 5397 794 654 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6865 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6830 DraI TTT'AAA Cuts at: 4169 4169 Size: 6865 DraIII CAC_nnn'GTG Cuts at: 2668 3871 2668 Size: 1203 5662 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2732 5872 809 Size: 507 1416 3140 1802 Fragments arranged by size: 3140 1802 1416 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3212 4312 5201 Size: 54 1425 50 275 18 1382 1100 889 Cuts at: 5201 6830 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1382 1100 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3212 4312 1537 Size: 275 1400 1100 4090 Fragments arranged by size: 4090 1400 1100 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2387 2408 3720 4101 4795 1353 Size: 1034 21 1312 381 694 3423 Fragments arranged by size: 3423 1312 1034 694 381 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3829 3956 5452 5826 5972 387 Size: 1064 138 2240 127 1496 374 146 1280 Fragments arranged by size: 2240 1496 1280 1064 374 146 138 127 EcoICRI GAG'CTC Cuts at: 2179 2217 3819 2179 Size: 38 1602 5225 Fragments arranged by size: 5225 1602 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2105 2189 2203 2742 2867 2982 3090 Size: 393 473 84 14 539 125 115 108 Cuts at: 3090 3507 4056 4125 4928 5525 6312 6580 1239 Size: 417 549 69 803 597 787 268 1524 Fragments arranged by size: 1524 803 787 597 549 539 473 417 393 268 125 115 108 84 69 14 EcoNI CCTnn'n_nnAGG Cuts at: 2252 3800 2252 Size: 1548 5317 EcoO109I rG'GnC_Cy Cuts at: 2209 2893 2952 2953 3349 3532 3580 3600 3919 Size: 684 59 1 396 183 48 20 319 Cuts at: 3919 4006 4014 2209 Size: 87 8 5060 Fragments arranged by size: 5060 684 396 319 183 87 59 48 20 8 1 EcoRI G'AATT_C Cuts at: 4174 4552 4174 Size: 378 6487 FseI GG_CCGG'CC Cuts at: 3214 3214 Size: 6865 FspI TGC'GCA Cuts at: 5371 5371 Size: 6865 HaeII r_GCGC'y Cuts at: 1678 3280 3881 6012 1678 Size: 1602 601 2131 2531 Fragments arranged by size: 2531 2131 1602 601 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2697 2729 2872 2904 3017 Size: 32 135 32 1125 32 143 32 113 Cuts at: 3017 3049 3294 3326 3584 3616 5506 5538 5580 Size: 32 245 32 258 32 1890 32 42 Cuts at: 5580 5612 1373 Size: 32 2626 Fragments arranged by size: 2626 1890 1125 258 245 143 135 113 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4209 4455 669 Size: 988 281 2271 246 3079 Fragments arranged by size: 3079 2271 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6865 HpaI GTT'AAC Cuts at: 4455 4455 Size: 6865 KasI G'GCGC_C Cuts at: 3276 3276 Size: 6865 KpnI G_GTAC'C Cuts at: 2401 3322 4190 2401 Size: 921 868 5076 Fragments arranged by size: 5076 921 868 MfeI C'AATT_G Cuts at: 2653 4464 2653 Size: 1811 5054 MluI A'CGCG_T Cuts at: 6741 6741 Size: 6865 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2433 3125 3390 4174 5979 6163 1305 Size: 1128 692 265 784 1805 184 2007 Fragments arranged by size: 2007 1805 1128 784 692 265 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5043 Fragments arranged by size: 5043 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2415 2481 3482 4823 5182 5341 518 Size: 648 1249 66 1001 1341 359 159 2042 Fragments arranged by size: 2042 1341 1249 1001 648 359 159 66 MspA1I CmG'CkG Cuts at: 1606 2101 2348 2492 2614 3285 4281 4958 6106 Size: 495 247 144 122 671 996 677 1148 Cuts at: 6106 6351 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 996 677 671 495 247 245 144 122 NaeI GCC'GGC Cuts at: 3185 3212 3185 Size: 27 6838 NarI GG'CG_CC Cuts at: 3277 3277 Size: 6865 NcoI C'CATG_G Cuts at: 513 1065 2004 3646 513 Size: 552 939 1642 3732 Fragments arranged by size: 3732 1642 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6865 NgoMIV G'CCGG_C Cuts at: 3183 3210 3183 Size: 27 6838 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6865 NotI GC'GGCC_GC Cuts at: 4312 4312 Size: 6865 NspI r_CATG'y Cuts at: 1132 1229 2433 3481 4223 5768 1132 Size: 97 1204 1048 742 1545 2229 Fragments arranged by size: 2229 1545 1204 1048 742 97 PasI CC'CwG_GG Cuts at: 1348 2949 1348 Size: 1601 5264 PciI A'CATG_T Cuts at: 5764 5764 Size: 6865 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6865 PfoI T'CCnGG_A Cuts at: 2895 3001 2895 Size: 106 6759 PmeI GTTT'AAAC Cuts at: 4169 4169 Size: 6865 PmlI CAC'GTG Cuts at: 2533 2533 Size: 6865 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4934 4966 6447 6479 4934 Size: 32 1481 32 5320 Fragments arranged by size: 5320 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2893 3580 3600 3919 2893 Size: 687 20 319 5839 Fragments arranged by size: 5839 687 319 20 PshAI GACnn'nnGTC Cuts at: 1613 3132 1613 Size: 1519 5346 PsiI TTA'TAA Cuts at: 4435 4435 Size: 6865 PspOMI G'GGCC_C Cuts at: 2952 3265 3531 2952 Size: 313 266 6286 Fragments arranged by size: 6286 313 266 PspXI vC'TCGA_Gb Cuts at: 2141 2600 2141 Size: 459 6406 PstI C_TGCA'G Cuts at: 830 2076 2528 2712 3667 3909 3918 4217 830 Size: 1246 452 184 955 242 9 299 3478 Fragments arranged by size: 3478 1246 955 452 299 242 184 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6865 PvuII CAG'CTG Cuts at: 2101 2348 2614 2101 Size: 247 266 6352 Fragments arranged by size: 6352 266 247 SacI G_AGCT'C Cuts at: 2181 2219 3821 2181 Size: 38 1602 5225 Fragments arranged by size: 5225 1602 38 SalI G'TCGA_C Cuts at: 1936 4207 1936 Size: 2271 4594 SanDI GG'GwC_CC Cuts at: 3600 3600 Size: 6865 SapI GCTCTTCn'nnn_ Cuts at: 4101 4101 Size: 6865 SbfI CC_TGCA'GG Cuts at: 2076 4217 2076 Size: 2141 4724 ScaI AGT'ACT Cuts at: 5113 6825 5113 Size: 1712 5153 SfcI C'TryA_G Cuts at: 826 1045 2072 2225 2524 2708 3370 3559 3663 Size: 219 1027 153 299 184 662 189 104 Cuts at: 3663 3881 3905 3914 4213 5348 6029 6220 826 Size: 218 24 9 299 1135 681 191 1471 Fragments arranged by size: 1471 1135 1027 681 662 299 299 219 218 191 189 184 153 104 24 9 SfoI GGC'GCC Cuts at: 3278 3278 Size: 6865 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6865 SgrAI Cr'CCGG_yG Cuts at: 1700 3183 1700 Size: 1483 5382 SmaI CCC'GGG Cuts at: 1872 4192 1872 Size: 2320 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2141 2600 3296 3627 3893 4969 Size: 197 926 198 459 696 331 266 1076 Cuts at: 4969 5870 6132 820 Size: 901 262 1553 Fragments arranged by size: 1553 1076 926 901 696 459 331 266 262 198 197 SnaBI TAC'GTA Cuts at: 493 4656 493 Size: 4163 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6865 SphI G_CATG'C Cuts at: 1132 2433 4223 1132 Size: 1301 1790 3774 Fragments arranged by size: 3774 1790 1301 SspI AAT'ATT Cuts at: 5 52 4789 5 Size: 47 4737 2081 Fragments arranged by size: 4737 2081 47 StuI AGG'CCT Cuts at: 1517 3162 1517 Size: 1645 5220 StyI C'CwwG_G Cuts at: 513 1065 2004 2296 2571 3037 3646 3778 4304 Size: 552 939 292 275 466 609 132 526 Cuts at: 4304 513 Size: 3074 Fragments arranged by size: 3074 939 609 552 526 466 292 275 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2699 3437 3472 3584 3926 4892 4909 Size: 1255 1119 738 35 112 342 966 17 Cuts at: 4909 5062 5247 5692 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1119 966 738 445 342 185 153 112 35 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3432 3996 5111 Size: 274 80 33 51 1443 1455 564 1115 Cuts at: 5111 6823 96 Size: 1712 138 Fragments arranged by size: 1712 1455 1443 1115 564 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2654 2674 2686 2706 6825 6857 2654 Size: 20 12 20 4119 32 2662 Fragments arranged by size: 4119 2662 32 20 20 12 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6865 XbaI T'CTAG_A Cuts at: 4201 4201 Size: 6865 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2578 3373 2578 Size: 795 6070 XhoI C'TCGA_G Cuts at: 1943 2141 2600 1943 Size: 198 459 6208 Fragments arranged by size: 6208 459 198 XmaI C'CCGG_G Cuts at: 1870 4190 1870 Size: 2320 4545 XmnI GAAnn'nnTTC Cuts at: 4994 4994 Size: 6865 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2968 4673 276 Size: 53 83 186 936 1434 1705 2468 Fragments arranged by size: 2468 1705 1434 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I EcoRV FalI FspAI NruI NsiI PacI PsrI RsrII SacII SexAI SfiI SrfI SwaI