(Linear) (Six-base) MAPSORT of: KIBB5712.seq Check: 6350 from: 1 to: 2160 KIBB5712.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 591 735 1665 2160 Size: 591 144 930 495 Fragments arranged by size: 930 591 495 144 AatII G_ACGT'C Cuts at: 0 965 2160 Size: 965 1195 Acc65I G'GTAC_C Cuts at: 0 392 1313 2160 Size: 392 921 847 Fragments arranged by size: 921 847 392 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 862 977 2051 2120 2160 Size: 862 115 1074 69 40 Fragments arranged by size: 1074 862 115 69 40 AgeI A'CCGG_T Cuts at: 0 6 2160 Size: 6 2154 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 127 1495 2160 Size: 127 1368 665 Fragments arranged by size: 1368 665 127 AlwNI CAG_nnn'CTG Cuts at: 0 207 490 891 1024 1791 1908 2160 Size: 207 283 401 133 767 117 252 Fragments arranged by size: 767 401 283 252 207 133 117 ApaI G_GGCC'C Cuts at: 0 951 1264 1530 2160 Size: 951 313 266 630 Fragments arranged by size: 951 630 313 266 ApaLI G'TGCA_C Cuts at: 0 1858 2160 Size: 1858 302 ApoI r'AATT_y Cuts at: 0 632 2160 Size: 632 1528 AvaI C'yCGr_G Cuts at: 0 136 595 1538 2160 Size: 136 459 943 622 Fragments arranged by size: 943 622 459 136 AvrII C'CTAG_G Cuts at: 0 1773 2160 Size: 1773 387 BanI G'GyrC_C Cuts at: 0 392 1242 1271 1313 2160 Size: 392 850 29 42 847 Fragments arranged by size: 850 847 392 42 29 BanII G_rGCy'C Cuts at: 0 176 214 916 951 1046 1109 1264 1530 Size: 176 38 702 35 95 63 155 266 Cuts at: 1530 1816 2160 Size: 286 344 Fragments arranged by size: 702 344 286 266 176 155 95 63 38 35 BbeI G_GCGC'C Cuts at: 0 1275 2160 Size: 1275 885 BbsI GAAGACnn'nnnn_ Cuts at: 0 965 974 1553 2160 Size: 965 9 579 607 Fragments arranged by size: 965 607 579 9 BbvCI CC'TCA_GC Cuts at: 0 1340 1750 1841 2160 Size: 1340 410 91 319 Fragments arranged by size: 1340 410 319 91 BclI T'GATC_A Cuts at: 0 1386 2160 Size: 1386 774 BglII A'GATC_T Cuts at: 0 1464 2160 Size: 1464 696 BlpI GC'TnA_GC Cuts at: 0 385 457 1171 2160 Size: 385 72 714 989 Fragments arranged by size: 989 714 385 72 Bme1580I G_kGCm'C Cuts at: 0 505 951 1264 1530 1862 2160 Size: 505 446 313 266 332 298 Fragments arranged by size: 505 446 332 313 298 266 BmrI ACTGGGnnnn_n' Cuts at: 0 986 2160 Size: 986 1174 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2010 2160 Size: 2010 150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 184 198 737 1085 1502 2160 Size: 100 84 14 539 348 417 658 Fragments arranged by size: 658 539 417 348 100 84 14 Bpu10I CC'TnA_GC Cuts at: 0 208 1040 1340 1750 1841 1940 2160 Size: 208 832 300 410 91 99 220 Fragments arranged by size: 832 410 300 220 208 99 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1276 1607 1873 2160 Size: 1276 331 266 287 Fragments arranged by size: 1276 331 287 266 BsaI GGTCTCn'nnnn_ Cuts at: 0 7 161 856 1555 1829 2160 Size: 7 154 695 699 274 331 Fragments arranged by size: 699 695 331 274 154 7 BsaAI yAC'GTr Cuts at: 0 528 2160 Size: 528 1632 BsaHI Gr'CG_yC Cuts at: 0 368 877 962 1272 2160 Size: 368 509 85 310 888 Fragments arranged by size: 888 509 368 310 85 BsaWI w'CCGG_w Cuts at: 0 6 858 2160 Size: 6 852 1302 Fragments arranged by size: 1302 852 6 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 385 415 710 740 1012 1042 2160 Size: 385 30 295 30 272 30 1118 Fragments arranged by size: 1118 385 295 272 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 364 466 775 924 2160 Size: 364 102 309 149 1236 Fragments arranged by size: 1236 364 309 149 102 BseYI C'CCAG_C Cuts at: 0 532 1003 1068 1080 1200 1591 1612 1627 Size: 532 471 65 12 120 391 21 15 Cuts at: 1627 1929 2160 Size: 302 231 Fragments arranged by size: 532 471 391 302 231 120 65 21 15 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 359 625 2160 Size: 359 266 1535 Fragments arranged by size: 1535 359 266 BsiEI CG_ry'CG Cuts at: 0 1210 2160 Size: 1210 950 BsiHKAI G_wGCw'C Cuts at: 0 176 214 596 1292 1816 1862 1885 2108 Size: 176 38 382 696 524 46 23 223 Cuts at: 2108 2160 Size: 52 Fragments arranged by size: 696 524 382 223 176 52 46 38 23 BsmBI CGTCTCn'nnnn_ Cuts at: 0 713 2160 Size: 713 1447 Bsp1286I G_dGCh'C Cuts at: 0 176 214 505 596 916 951 1046 1109 Size: 176 38 291 91 320 35 95 63 Cuts at: 1109 1264 1292 1530 1816 1862 1885 2108 2160 Size: 155 28 238 286 46 23 223 52 Fragments arranged by size: 320 291 286 238 223 176 155 95 91 63 52 46 38 35 28 23 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 119 242 380 591 735 1504 1665 1902 Size: 119 123 138 211 144 769 161 237 Cuts at: 1902 2160 Size: 258 Fragments arranged by size: 769 258 237 211 161 144 138 123 119 BsrFI r'CCGG_y Cuts at: 0 6 1178 1205 2160 Size: 6 1172 27 955 Fragments arranged by size: 1172 955 27 6 BsrGI T'GTAC_A Cuts at: 0 1427 2160 Size: 1427 733 BstAPI GCAn_nnn'nTGC Cuts at: 0 1866 1908 2160 Size: 1866 42 252 Fragments arranged by size: 1866 252 42 BstEII G'GTnAC_C Cuts at: 0 1933 2160 Size: 1933 227 BstXI CCAn_nnnn'nTGG Cuts at: 0 532 2160 Size: 532 1628 BstYI r'GATC_y Cuts at: 0 434 515 536 707 848 1464 2160 Size: 434 81 21 171 141 616 696 Fragments arranged by size: 696 616 434 171 141 81 21 Bsu36I CC'TnA_GG Cuts at: 0 511 884 2160 Size: 511 373 1276 Fragments arranged by size: 1276 511 373 BtgI C'CryG_G Cuts at: 0 1641 1684 2160 Size: 1641 43 476 Fragments arranged by size: 1641 476 43 BtsI GCAGTG_nn' Cuts at: 0 1722 2160 Size: 1722 438 DraIII CAC_nnn'GTG Cuts at: 0 663 1866 2160 Size: 663 1203 294 Fragments arranged by size: 1203 663 294 DrdI GACnn_nn'nnGTC Cuts at: 0 727 2160 Size: 727 1433 EaeI y'GGCC_r Cuts at: 0 1207 2160 Size: 1207 953 EagI C'GGCC_G Cuts at: 0 1207 2160 Size: 1207 953 EarI CTCTTCn'nnn_ Cuts at: 0 382 403 1715 2096 2160 Size: 382 21 1312 381 64 Fragments arranged by size: 1312 382 381 64 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1824 1951 2160 Size: 1824 127 209 Fragments arranged by size: 1824 209 127 EcoICRI GAG'CTC Cuts at: 0 174 212 1814 2160 Size: 174 38 1602 346 Fragments arranged by size: 1602 346 174 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 100 184 198 737 862 977 1085 1502 Size: 100 84 14 539 125 115 108 417 Cuts at: 1502 2051 2120 2160 Size: 549 69 40 Fragments arranged by size: 549 539 417 125 115 108 100 84 69 40 14 EcoNI CCTnn'n_nnAGG Cuts at: 0 247 1795 2160 Size: 247 1548 365 Fragments arranged by size: 1548 365 247 EcoO109I rG'GnC_Cy Cuts at: 0 204 888 947 948 1344 1527 1575 1595 Size: 204 684 59 1 396 183 48 20 Cuts at: 1595 1914 2001 2009 2160 Size: 319 87 8 151 Fragments arranged by size: 684 396 319 204 183 151 87 59 48 20 8 1 FseI GG_CCGG'CC Cuts at: 0 1209 2160 Size: 1209 951 HaeII r_GCGC'y Cuts at: 0 1275 1876 2160 Size: 1275 601 284 Fragments arranged by size: 1275 601 284 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 692 724 867 899 1012 1044 1289 1321 Size: 692 32 143 32 113 32 245 32 Cuts at: 1321 1579 1611 2160 Size: 258 32 549 Fragments arranged by size: 692 549 258 245 143 113 32 32 32 32 32 KasI G'GCGC_C Cuts at: 0 1271 2160 Size: 1271 889 KpnI G_GTAC'C Cuts at: 0 396 1317 2160 Size: 396 921 843 Fragments arranged by size: 921 843 396 MfeI C'AATT_G Cuts at: 0 648 2160 Size: 648 1512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 428 1120 1385 2160 Size: 428 692 265 775 Fragments arranged by size: 775 692 428 265 MslI CAynn'nnrTG Cuts at: 0 410 476 1477 2160 Size: 410 66 1001 683 Fragments arranged by size: 1001 683 410 66 MspA1I CmG'CkG Cuts at: 0 96 343 487 609 1280 2160 Size: 96 247 144 122 671 880 Fragments arranged by size: 880 671 247 144 122 96 NaeI GCC'GGC Cuts at: 0 1180 1207 2160 Size: 1180 27 953 Fragments arranged by size: 1180 953 27 NarI GG'CG_CC Cuts at: 0 1272 2160 Size: 1272 888 NcoI C'CATG_G Cuts at: 0 1641 2160 Size: 1641 519 NgoMIV G'CCGG_C Cuts at: 0 1178 1205 2160 Size: 1178 27 955 Fragments arranged by size: 1178 955 27 NspI r_CATG'y Cuts at: 0 428 1476 2160 Size: 428 1048 684 Fragments arranged by size: 1048 684 428 PasI CC'CwG_GG Cuts at: 0 944 2160 Size: 944 1216 PfoI T'CCnGG_A Cuts at: 0 890 996 2160 Size: 890 106 1164 Fragments arranged by size: 1164 890 106 PmlI CAC'GTG Cuts at: 0 528 2160 Size: 528 1632 PpuMI rG'GwC_Cy Cuts at: 0 888 1575 1595 1914 2160 Size: 888 687 20 319 246 Fragments arranged by size: 888 687 319 246 20 PshAI GACnn'nnGTC Cuts at: 0 1127 2160 Size: 1127 1033 PspOMI G'GGCC_C Cuts at: 0 947 1260 1526 2160 Size: 947 313 266 634 Fragments arranged by size: 947 634 313 266 PspXI vC'TCGA_Gb Cuts at: 0 136 595 2160 Size: 136 459 1565 Fragments arranged by size: 1565 459 136 PstI C_TGCA'G Cuts at: 0 71 523 707 1662 1904 1913 2160 Size: 71 452 184 955 242 9 247 Fragments arranged by size: 955 452 247 242 184 71 9 PvuII CAG'CTG Cuts at: 0 96 343 609 2160 Size: 96 247 266 1551 Fragments arranged by size: 1551 266 247 96 SacI G_AGCT'C Cuts at: 0 176 214 1816 2160 Size: 176 38 1602 344 Fragments arranged by size: 1602 344 176 38 SanDI GG'GwC_CC Cuts at: 0 1595 2160 Size: 1595 565 SapI GCTCTTCn'nnn_ Cuts at: 0 2096 2160 Size: 2096 64 SbfI CC_TGCA'GG Cuts at: 0 71 2160 Size: 71 2089 SfcI C'TryA_G Cuts at: 0 67 220 519 703 1365 1554 1658 1876 Size: 67 153 299 184 662 189 104 218 Cuts at: 1876 1900 1909 2160 Size: 24 9 251 Fragments arranged by size: 662 299 251 218 189 184 153 104 67 24 9 SfoI GGC'GCC Cuts at: 0 1273 2160 Size: 1273 887 SgrAI Cr'CCGG_yG Cuts at: 0 1178 2160 Size: 1178 982 SmlI C'TyrA_G Cuts at: 0 136 595 1291 1622 1888 2160 Size: 136 459 696 331 266 272 Fragments arranged by size: 696 459 331 272 266 136 SphI G_CATG'C Cuts at: 0 428 2160 Size: 428 1732 StuI AGG'CCT Cuts at: 0 1157 2160 Size: 1157 1003 StyI C'CwwG_G Cuts at: 0 291 566 1032 1641 1773 2160 Size: 291 275 466 609 132 387 Fragments arranged by size: 609 466 387 291 275 132 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 694 1432 1467 1579 1921 2160 Size: 694 738 35 112 342 239 Fragments arranged by size: 738 694 342 239 112 35 TatI w'GTAC_w Cuts at: 0 1427 1991 2160 Size: 1427 564 169 Fragments arranged by size: 1427 564 169 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 649 669 681 701 2160 Size: 649 20 12 20 1459 Fragments arranged by size: 1459 649 20 20 12 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 573 1368 2160 Size: 573 795 792 Fragments arranged by size: 795 792 573 XhoI C'TCGA_G Cuts at: 0 136 595 2160 Size: 136 459 1565 Fragments arranged by size: 1565 459 136 ZraI GAC'GTC Cuts at: 0 963 2160 Size: 963 1197 Enzymes that do cut: AarI AatII Acc65I AcuI AgeI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KasI KpnI MfeI MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI PvuII SacI SanDI SapI SbfI SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII TatI TstI XcmI XhoI ZraI Enzymes that do not cut: AccI AclI AfeI AflII AflIII AhdI AleI AloI AscI AseI AsiSI BaeI BamHI BcgI BciVI BfrBI BglI BmgBI BmtI BsaBI BsiWI BsmI BspEI BspHI BsrBI BsrDI BssHII BssSI BstBI BstZ17I BtgZI ClaI CspCI DraI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI MluI MscI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PpiI PsiI PsrI PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI XmnI