################################## # NO CUT ENZYMES ( 154 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanI BbeI BbsI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BsrI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RcaI RsaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TfiI Tsp509I Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### BbrPI 138 CACGTG CAC^GTG BbvCI 66 GCTGAGG CCTCAGC(-5/-2) BcgI 504 CGAGCTGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BsaBI 84 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 107 ACCGGA [AT]^CCGG[AT] BseRI 238 GAGGAG GAGGAG(10/8) BsmAI 193 GAGAC GTCTC(1/5) BspMI 809 ACCTGC ACCTGC(4/8) BsrDI 427 GCAATG GCAATG(2/0) BstF5I 264 CATCC GGATG(2/0) BstXI 665 CCACTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 386 CCATGG C^C[GA][CT]GG FokI 264 CATCC GGATG(9/13) HphI 681 TCACC GGTGA(8/7) MamI 84 GATGGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 146 TTAA T^TAA NcoI 386 CCATGG C^CATGG NheI 6 GCTAGC G^CTAGC PmaCI 138 CACGTG CAC^GTG PmlI 138 CACGTG CAC^GTG PstI 469 CTGCAG CTGCA^G SapI 403 GAAGAGC GCTCTTC(1/4) SmlI 739 CTCAAG C^T[CT][GA]AG StuI 254 AGGCCT AGG^CCT TaqI 734 TCGA T^CGA Tru9I 146 TTAA T^TAA TspRI 204 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 734 TCGA T^CGA XcmI 584 CCACCTGTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AviII 50 TGCGCA TGC^GCA 812 TGCGCA BalI 86 TGGCCA TGG^CCA 605 TGGCCA BfaI 7 CTAG C^TAG 134 CTAG BsaAI 138 CACGTG [CT]AC^GT[GA] 717 TACGTG BssSI 502 CACGAG CACGAG(-5/-1) 409 CTCGTG DpnII 459 GATC ^GATC 477 GATC EaeI 86 TGGCCA [CT]^GGCC[GA] 605 TGGCCA EarI 190 GAAGAG CTCTTC(1/4) 403 GAAGAG EcoT14I 257 CCTTGG C^C[AT][AT]GG 386 CCATGG FspI 50 TGCGCA TGC^GCA 812 TGCGCA HaeII 572 AGCGCC [GA]GCGC^[CT] 818 AGCGCC HapII 108 CCGG C^CGG 360 CCGG HpaII 108 CCGG C^CGG 360 CCGG Ksp632I 190 GAAGAG CTCTTC(1/4) 403 GAAGAG MaeI 7 CTAG C^TAG 134 CTAG MaeII 139 ACGT A^CGT 718 ACGT MaeIII 206 GTGAC ^GT[ACGT]AC 545 GTGAC MboI 459 GATC ^GATC 477 GATC MluNI 86 TGGCCA TGG^CCA 605 TGGCCA MscI 86 TGGCCA TGG^CCA 605 TGGCCA MslI 585 CACCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 666 CACTTGCATG MspI 108 CCGG C^CGG 360 CCGG NdeII 459 GATC ^GATC 477 GATC NspI 47 ACATGC [GA]CATG^[CT] 623 ACATGC PflMI 210 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 381 CCAGCCCATGG PpuMI 464 AGGTCCT [GA]G^G[AT]CC[CT] 773 AGGTCCT Sau3AI 459 GATC ^GATC 477 GATC StyI 257 CCTTGG C^C[AT][AT]GG 386 CCATGG Tsp45I 206 GTGAC ^GT[GC]AC 545 GTGAC Van91I 210 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 381 CCAGCCCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AlwNI ApaI AspHI AvaII BanII BbvI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I DdeI DraII EcoO109I EcoRII Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboII MnlI MvaI MwoI NlaIII NlaIV PleI SacI Sau96I ScrFI SexAI SfaNI SfcI TseI