(Circular) (Six-base) MAPSORT of: pFN21AE6444.seq Check: 5135 from: 1 to: 6235 pFN21AE6444 6235 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3019 3019 Size: 6235 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3578 1937 Size: 1641 4594 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4298 5682 5950 1632 Size: 2666 1384 268 1917 Fragments arranged by size: 2666 1917 1384 268 AfeI AGC'GCT Cuts at: 2244 2244 Size: 6235 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 2727 5134 6111 1203 Size: 1524 2407 977 1327 Fragments arranged by size: 2407 1524 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 3040 3530 4964 3040 Size: 490 1434 4311 Fragments arranged by size: 4311 1434 490 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2372 2530 1184 Size: 1188 158 4889 Fragments arranged by size: 4889 1188 158 AlwNI CAG_nnn'CTG Cuts at: 2497 2665 3300 3398 3978 5550 2497 Size: 168 635 98 580 1572 3182 Fragments arranged by size: 3182 1572 635 580 168 98 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 3544 3770 3922 6031 1493 Size: 2051 226 152 2109 1697 Fragments arranged by size: 2109 2051 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2208 3323 3560 1870 Size: 73 265 1115 237 4545 Fragments arranged by size: 4545 1115 265 237 73 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2088 2228 2443 2635 3556 Size: 325 217 627 301 140 215 192 921 Cuts at: 3556 5011 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 921 627 325 301 217 215 192 140 BanII G_rGCy'C Cuts at: 1391 2209 2279 3324 3554 1391 Size: 818 70 1045 230 4072 Fragments arranged by size: 4072 1045 818 230 70 BbeI G_GCGC'C Cuts at: 2232 2232 Size: 6235 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2772 3021 928 Size: 971 873 249 4142 Fragments arranged by size: 4142 971 873 249 BbvCI CC'TCA_GC Cuts at: 2523 2742 2523 Size: 219 6016 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4426 4460 1903 Size: 34 50 34 2405 34 3678 Fragments arranged by size: 3678 2405 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 1548 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4846 136 Size: 107 122 71 631 605 3174 1525 Fragments arranged by size: 3174 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6230 6230 Size: 6235 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2093 4296 5452 1790 Size: 303 2203 1156 2573 Fragments arranged by size: 2573 2203 1156 303 BmgBI CAC'GTC Cuts at: 1318 2119 1318 Size: 801 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2868 3966 4924 Size: 416 53 83 399 24 1451 1098 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1451 1098 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2822 2986 2822 Size: 164 6071 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2126 2681 3375 4895 1239 Size: 887 555 694 1520 2579 Fragments arranged by size: 2579 1520 887 694 555 Bpu10I CC'TnA_GC Cuts at: 2523 2742 2523 Size: 219 6016 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2792 2956 3269 4360 5225 5523 2792 Size: 164 313 1091 865 298 3504 Fragments arranged by size: 3504 1091 865 313 298 164 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 4898 882 Size: 617 603 2796 2219 Fragments arranged by size: 2796 2219 617 603 BsaAI yAC'GTr Cuts at: 493 1204 2728 4026 493 Size: 711 1524 1298 2702 Fragments arranged by size: 2702 1524 1298 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2229 4042 4424 5059 Size: 53 83 186 936 696 1813 382 635 Cuts at: 5059 275 Size: 1451 Fragments arranged by size: 1813 1451 936 696 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3198 4668 5066 5340 5487 5898 3198 Size: 1470 398 274 147 411 3535 Fragments arranged by size: 3535 1470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6010 Fragments arranged by size: 6010 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2959 3179 3182 3257 3425 3428 1186 Size: 1773 220 3 75 168 3 3993 Fragments arranged by size: 3993 1773 220 168 75 3 3 BseYI C'CCAG_C Cuts at: 2134 2533 2540 3141 5438 5934 2134 Size: 399 7 601 2297 496 2435 Fragments arranged by size: 2435 2297 601 496 399 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2234 2378 2500 1163 Size: 573 498 144 122 4898 Fragments arranged by size: 4898 573 498 144 122 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3685 4446 5474 1540 Size: 275 186 1684 761 1028 2301 Fragments arranged by size: 2301 1684 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2343 2931 3114 4296 4381 5452 2343 Size: 588 183 1182 85 1071 3126 Fragments arranged by size: 3126 1182 1071 588 183 85 BsmI GAATG_Cn' Cuts at: 3208 3746 3839 3208 Size: 538 93 5604 Fragments arranged by size: 5604 538 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2209 2279 2343 2931 3114 3324 Size: 399 303 116 70 64 588 183 210 Cuts at: 3324 3554 4296 4381 5452 1391 Size: 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 399 303 230 210 183 116 85 70 64 BspEI T'CCGG_A Cuts at: 3198 5898 3198 Size: 2700 3535 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3019 3590 844 Size: 2175 571 3489 Fragments arranged by size: 3489 2175 571 BsrBI CCG'CTC Cuts at: 1394 1823 2604 4122 1394 Size: 429 781 1518 3507 Fragments arranged by size: 3507 1518 781 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2965 3505 4730 4904 65 Size: 1183 1717 540 1225 174 1396 Fragments arranged by size: 1717 1396 1225 1183 540 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2085 2405 4879 5066 1700 Size: 110 275 320 2474 187 2869 Fragments arranged by size: 2869 2474 320 275 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6235 BssSI C'ACGA_G Cuts at: 4295 5307 4295 Size: 1012 5223 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6235 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstYI r'GATC_y Cuts at: 1972 3565 3932 4317 4334 5786 5872 5884 6230 Size: 1593 367 385 17 1452 86 12 346 Cuts at: 6230 1972 Size: 1977 Fragments arranged by size: 1977 1593 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2040 2040 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 2094 3351 3492 513 Size: 552 1029 1257 141 3256 Fragments arranged by size: 3256 1257 1029 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5496 Fragments arranged by size: 5496 683 56 BtsI GCAGTG_nn' Cuts at: 2361 3751 4545 4565 2361 Size: 1390 794 20 4031 Fragments arranged by size: 4031 1390 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2759 2794 529 Size: 35 2195 35 3970 Fragments arranged by size: 3970 2195 35 35 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DraIII CAC_nnn'GTG Cuts at: 2635 2635 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2561 5242 809 Size: 507 1245 2681 1802 Fragments arranged by size: 2681 1802 1245 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3682 4571 6200 Size: 54 1425 50 275 18 1852 889 1629 Cuts at: 6200 8 Size: 43 Fragments arranged by size: 1852 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3682 1537 Size: 275 1870 4090 Fragments arranged by size: 4090 1870 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2478 3312 4165 1353 Size: 1125 834 853 3423 Fragments arranged by size: 3423 1125 853 834 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2378 4822 5196 5342 387 Size: 1064 138 789 2444 374 146 1280 Fragments arranged by size: 2444 1280 1064 789 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2126 2681 3375 4298 4895 5682 5950 Size: 393 494 555 694 923 597 787 268 Cuts at: 5950 1239 Size: 1524 Fragments arranged by size: 1524 923 787 694 597 555 494 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2696 2741 2752 2696 Size: 45 11 6179 Fragments arranged by size: 6179 45 11 EcoO109I rG'GnC_Cy Cuts at: 2036 2494 2758 3474 2036 Size: 458 264 716 4797 Fragments arranged by size: 4797 716 458 264 EcoRI G'AATT_C Cuts at: 3544 3922 3544 Size: 378 5857 FspI TGC'GCA Cuts at: 2348 4741 2348 Size: 2393 3842 HaeII r_GCGC'y Cuts at: 1678 2232 2246 5382 1678 Size: 554 14 3136 2531 Fragments arranged by size: 3136 2531 554 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3026 3058 3516 3548 4876 Size: 32 135 32 1454 32 458 32 1328 Cuts at: 4876 4908 4950 4982 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1454 1328 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3579 3825 669 Size: 988 281 1641 246 3079 Fragments arranged by size: 3079 1641 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KasI G'GCGC_C Cuts at: 2228 2228 Size: 6235 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5349 5533 1305 Size: 4044 184 2007 Fragments arranged by size: 4044 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4193 4552 4711 518 Size: 648 3027 359 159 2042 Fragments arranged by size: 3027 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2096 2522 2934 3651 4328 5476 5721 1606 Size: 490 426 412 717 677 1148 245 2120 Fragments arranged by size: 2120 1148 717 677 490 426 412 245 NaeI GCC'GGC Cuts at: 2407 2407 Size: 6235 NarI GG'CG_CC Cuts at: 2229 2229 Size: 6235 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5683 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NgoMIV G'CCGG_C Cuts at: 2405 2405 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3593 5138 1132 Size: 97 2364 1545 2229 Fragments arranged by size: 2364 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2017 1348 Size: 669 5566 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 3398 1108 Size: 2290 3945 PfoI T'CCnGG_A Cuts at: 2697 2697 Size: 6235 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PmlI CAC'GTG Cuts at: 2728 2728 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3474 3474 Size: 6235 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2229 2261 2229 Size: 32 6203 PstI C_TGCA'G Cuts at: 830 3587 830 Size: 2757 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 PvuII CAG'CTG Cuts at: 2522 2522 Size: 6235 SacII CC_GC'GG Cuts at: 2097 2097 Size: 6235 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SanDI GG'GwC_CC Cuts at: 3474 3474 Size: 6235 SapI GCTCTTCn'nnn_ Cuts at: 3312 3312 Size: 6235 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SexAI A'CCwGG_T Cuts at: 3528 3528 Size: 6235 SfcI C'TryA_G Cuts at: 826 1045 3583 4718 5399 5590 826 Size: 219 2538 1135 681 191 1471 Fragments arranged by size: 2538 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2230 2230 Size: 6235 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3562 1872 Size: 1690 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2807 2971 3284 4339 5240 5502 Size: 197 926 864 164 313 1055 901 262 Cuts at: 5502 820 Size: 1553 Fragments arranged by size: 1553 1055 926 901 864 313 262 197 164 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 2045 2747 1517 Size: 528 702 5005 Fragments arranged by size: 5005 702 528 StyI C'CwwG_G Cuts at: 513 1065 3674 513 Size: 552 2609 3074 Fragments arranged by size: 3074 2609 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2729 3053 4262 4279 4432 4617 5062 Size: 1255 1149 324 1209 17 153 185 445 Cuts at: 5062 325 Size: 1498 Fragments arranged by size: 1498 1255 1209 1149 445 324 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4481 6193 96 Size: 274 80 33 51 1443 2504 1712 138 Fragments arranged by size: 2504 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6195 6227 6195 Size: 32 6203 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3017 3017 Size: 6235 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6235 XmaI C'CCGG_G Cuts at: 1870 3560 1870 Size: 1690 4545 XmnI GAAnn'nnTTC Cuts at: 4364 4364 Size: 6235 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PspOMI PspXI RsrII SacI SfiI SrfI SwaI