Restriction map for
pFN21AB5715
(Linear) (Six-base) MAPSORT of: KIBB5715.seq Check: 3374 from: 1 to: 2361
KIBB5715.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1091 1128 2137 2361
Size: 1091 37 1009 224
Fragments arranged by size:
1091 1009 224 37
AccI GT'mk_AC
Cuts at: 0 386 2361
Size: 386 1975
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1383 1749 2361
Size: 545 838 366 612
Fragments arranged by size:
838 612 545 366
AflIII A'CryG_T
Cuts at: 0 227 2361
Size: 227 2134
AleI CACnn'nnGTG
Cuts at: 0 754 1921 2361
Size: 754 1167 440
Fragments arranged by size:
1167 754 440
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 334 2361
Size: 334 2027
AlwNI CAG_nnn'CTG
Cuts at: 0 372 673 1758 2295 2361
Size: 372 301 1085 537 66
Fragments arranged by size:
1085 537 372 301 66
ApaI G_GGCC'C
Cuts at: 0 1796 1857 2361
Size: 1796 61 504
Fragments arranged by size:
1796 504 61
ApaLI G'TGCA_C
Cuts at: 0 2252 2361
Size: 2252 109
AvaI C'yCGr_G
Cuts at: 0 75 93 449 1059 1300 1536 2361
Size: 75 18 356 610 241 236 825
Fragments arranged by size:
825 610 356 241 236 75 18
BamHI G'GATC_C
Cuts at: 0 2235 2361
Size: 2235 126
BanI G'GyrC_C
Cuts at: 0 862 895 1039 2055 2149 2361
Size: 862 33 144 1016 94 212
Fragments arranged by size:
1016 862 212 144 94 33
BanII G_rGCy'C
Cuts at: 0 35 241 917 1736 1796 1829 1857 2222
Size: 35 206 676 819 60 33 28 365
Cuts at: 2222 2277 2361
Size: 55 84
Fragments arranged by size:
819 676 365 206 84 60 55 35
33 28
BbeI G_GCGC'C
Cuts at: 0 2059 2361
Size: 2059 302
BbvCI CC'TCA_GC
Cuts at: 0 1830 2114 2361
Size: 1830 284 247
Fragments arranged by size:
1830 284 247
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 559 2361
Size: 525 34 1802
Fragments arranged by size:
1802 525 34
BglI GCCn_nnn'nGGC
Cuts at: 0 191 712 976 2266 2291 2361
Size: 191 521 264 1290 25 70
Fragments arranged by size:
1290 521 264 191 70 25
BglII A'GATC_T
Cuts at: 0 1979 2361
Size: 1979 382
BlpI GC'TnA_GC
Cuts at: 0 181 674 2361
Size: 181 493 1687
Fragments arranged by size:
1687 493 181
Bme1580I G_kGCm'C
Cuts at: 0 587 867 1042 1796 1857 1940 2050 2256
Size: 587 280 175 754 61 83 110 206
Cuts at: 2256 2361
Size: 105
Fragments arranged by size:
754 587 280 206 175 110 105 83
61
BmgBI CAC'GTC
Cuts at: 0 627 2361
Size: 627 1734
BmrI ACTGGGnnnn_n'
Cuts at: 0 843 1667 2087 2216 2243 2361
Size: 843 824 420 129 27 118
Fragments arranged by size:
843 824 420 129 118 27
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 936 2361
Size: 936 1425
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1653 1916 2361
Size: 1653 263 445
Fragments arranged by size:
1653 445 263
Bpu10I CC'TnA_GC
Cuts at: 0 1830 2066 2114 2361
Size: 1830 236 48 247
Fragments arranged by size:
1830 247 236 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 1333 2202 2361
Size: 1333 869 159
Fragments arranged by size:
1333 869 159
BsaHI Gr'CG_yC
Cuts at: 0 2056 2361
Size: 2056 305
BsaWI w'CCGG_w
Cuts at: 0 335 2361
Size: 335 2026
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 153 183 274 304 2361
Size: 153 30 91 30 2057
Fragments arranged by size:
2057 153 91 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 162 1277 1833 1901 2037 2361
Size: 159 3 1115 556 68 136 324
Fragments arranged by size:
1115 556 324 159 136 68 3
BseYI C'CCAG_C
Cuts at: 0 15 66 353 424 1856 2161 2361
Size: 15 51 287 71 1432 305 200
Fragments arranged by size:
1432 305 287 200 71 51 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 516 565 714 908 1668 2361
Size: 489 27 49 149 194 760 693
Fragments arranged by size:
760 693 489 194 149 49 27
BsiEI CG_ry'CG
Cuts at: 0 110 195 2361
Size: 110 85 2166
Fragments arranged by size:
2166 110 85
BsiHKAI G_wGCw'C
Cuts at: 0 35 323 680 1235 1391 1600 1736 2256
Size: 35 288 357 555 156 209 136 520
Cuts at: 2256 2361
Size: 105
Fragments arranged by size:
555 520 357 288 209 156 136 105
35
BsmI GAATG_Cn'
Cuts at: 0 345 2361
Size: 345 2016
Bsp1286I G_dGCh'C
Cuts at: 0 35 241 323 587 680 867 917 1042
Size: 35 206 82 264 93 187 50 125
Cuts at: 1042 1235 1391 1600 1736 1796 1829 1857 1940
Size: 193 156 209 136 60 33 28 83
Cuts at: 1940 2050 2222 2256 2277 2361
Size: 110 172 34 21 84
Fragments arranged by size:
264 209 206 193 187 172 156 136
125 110 93 84 83 82 60 50
35 34 33 28 21
BspEI T'CCGG_A
Cuts at: 0 335 2361
Size: 335 2026
BspHI T'CATG_A
Cuts at: 0 1268 2361
Size: 1268 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1091 1128 1640 2137 2361
Size: 1091 37 512 497 224
Fragments arranged by size:
1091 512 497 224 37
BsrBI CCG'CTC
Cuts at: 0 39 98 480 660 2361
Size: 39 59 382 180 1701
Fragments arranged by size:
1701 382 180 59 39
BsrFI r'CCGG_y
Cuts at: 0 2256 2361
Size: 2256 105
BssSI C'ACGA_G
Cuts at: 0 316 1729 2361
Size: 316 1413 632
Fragments arranged by size:
1413 632 316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 647 1126 2361
Size: 647 479 1235
Fragments arranged by size:
1235 647 479
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 756 1875 2325 2361
Size: 273 483 1119 450 36
Fragments arranged by size:
1119 483 450 273 36
BstYI r'GATC_y
Cuts at: 0 1979 2235 2361
Size: 1979 256 126
Fragments arranged by size:
1979 256 126
BstZ17I GTA'TAC
Cuts at: 0 387 2361
Size: 387 1974
Bsu36I CC'TnA_GG
Cuts at: 0 2231 2361
Size: 2231 130
BtgI C'CryG_G
Cuts at: 0 1796 2264 2361
Size: 1796 468 97
Fragments arranged by size:
1796 468 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 684 1572 2361
Size: 684 888 789
Fragments arranged by size:
888 789 684
BtsI GCAGTG_nn'
Cuts at: 0 186 268 365 2361
Size: 186 82 97 1996
Fragments arranged by size:
1996 186 97 82
DraIII CAC_nnn'GTG
Cuts at: 0 1779 2361
Size: 1779 582
EaeI y'GGCC_r
Cuts at: 0 2 192 275 746 2280 2361
Size: 2 190 83 471 1534 81
Fragments arranged by size:
1534 471 190 83 81 2
EagI C'GGCC_G
Cuts at: 0 192 2361
Size: 192 2169
EarI CTCTTCn'nnn_
Cuts at: 0 667 742 805 1687 2014 2023 2361
Size: 667 75 63 882 327 9 338
Fragments arranged by size:
882 667 338 327 75 63 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 161 783 2361
Size: 161 622 1578
Fragments arranged by size:
1578 622 161
EcoICRI GAG'CTC
Cuts at: 0 33 1734 2361
Size: 33 1701 627
Fragments arranged by size:
1701 627 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 1383 1653 1749 1916 2361
Size: 545 838 270 96 167 445
Fragments arranged by size:
838 545 445 270 167 96
EcoNI CCTnn'n_nnAGG
Cuts at: 0 439 1647 2361
Size: 439 1208 714
Fragments arranged by size:
1208 714 439
EcoO109I rG'GnC_Cy
Cuts at: 0 72 454 2292 2299 2361
Size: 72 382 1838 7 62
Fragments arranged by size:
1838 382 72 62 7
HaeII r_GCGC'y
Cuts at: 0 2004 2059 2361
Size: 2004 55 302
Fragments arranged by size:
2004 302 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 153 185 274 306 863 895 919 951
Size: 153 32 89 32 557 32 24 32
Cuts at: 951 1135 1167 1515 1547 1828 1860 1863 1895
Size: 184 32 348 32 281 32 3 32
Cuts at: 1895 2335 2361
Size: 440 26
Fragments arranged by size:
557 440 348 281 184 153 89 32
32 32 32 32 32 32 32 26
24 3
HincII GTy'rAC
Cuts at: 0 1655 2361
Size: 1655 706
KasI G'GCGC_C
Cuts at: 0 2055 2361
Size: 2055 306
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2225 2361
Size: 2225 136
MscI TGG'CCA
Cuts at: 0 748 2361
Size: 748 1613
MslI CAynn'nnrTG
Cuts at: 0 754 1591 1870 1921 2361
Size: 754 837 279 51 440
Fragments arranged by size:
837 754 440 279 51
MspA1I CmG'CkG
Cuts at: 0 7 180 216 350 1403 1834 2052 2118
Size: 7 173 36 134 1053 431 218 66
Cuts at: 2118 2361
Size: 243
Fragments arranged by size:
1053 431 243 218 173 134 66 36
7
NarI GG'CG_CC
Cuts at: 0 2056 2361
Size: 2056 305
NspI r_CATG'y
Cuts at: 0 393 690 1773 1864 2361
Size: 393 297 1083 91 497
Fragments arranged by size:
1083 497 393 297 91
PasI CC'CwG_GG
Cuts at: 0 1035 2361
Size: 1035 1326
PflMI CCAn_nnn'nTGG
Cuts at: 0 614 855 1961 2361
Size: 614 241 1106 400
Fragments arranged by size:
1106 614 400 241
PfoI T'CCnGG_A
Cuts at: 0 1451 2361
Size: 1451 910
PpuMI rG'GwC_Cy
Cuts at: 0 72 2361
Size: 72 2289
PshAI GACnn'nnGTC
Cuts at: 0 2194 2361
Size: 2194 167
PspOMI G'GGCC_C
Cuts at: 0 1792 1853 2361
Size: 1792 61 508
Fragments arranged by size:
1792 508 61
PspXI vC'TCGA_Gb
Cuts at: 0 93 2361
Size: 93 2268
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 2361
Size: 1177 32 1152
Fragments arranged by size:
1177 1152 32
PstI C_TGCA'G
Cuts at: 0 1742 1997 2148 2361
Size: 1742 255 151 213
Fragments arranged by size:
1742 255 213 151
PvuII CAG'CTG
Cuts at: 0 1403 1834 2118 2361
Size: 1403 431 284 243
Fragments arranged by size:
1403 431 284 243
SacI G_AGCT'C
Cuts at: 0 35 1736 2361
Size: 35 1701 625
Fragments arranged by size:
1701 625 35
SanDI GG'GwC_CC
Cuts at: 0 72 2361
Size: 72 2289
SapI GCTCTTCn'nnn_
Cuts at: 0 667 742 1687 2361
Size: 667 75 945 674
Fragments arranged by size:
945 674 667 75
SexAI A'CCwGG_T
Cuts at: 0 1090 1642 2361
Size: 1090 552 719
Fragments arranged by size:
1090 719 552
SfcI C'TryA_G
Cuts at: 0 1738 1993 2144 2361
Size: 1738 255 151 217
Fragments arranged by size:
1738 255 217 151
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 712 2361
Size: 712 1649
SfoI GGC'GCC
Cuts at: 0 2057 2361
Size: 2057 304
SmaI CCC'GGG
Cuts at: 0 77 1302 2361
Size: 77 1225 1059
Fragments arranged by size:
1225 1059 77
SmlI C'TyrA_G
Cuts at: 0 93 2361
Size: 93 2268
SphI G_CATG'C
Cuts at: 0 1773 2361
Size: 1773 588
StuI AGG'CCT
Cuts at: 0 1714 2361
Size: 1714 647
StyI C'CwwG_G
Cuts at: 0 587 1613 1709 2361
Size: 587 1026 96 652
Fragments arranged by size:
1026 652 587 96
TatI w'GTAC_w
Cuts at: 0 962 2361
Size: 962 1399
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 2361
Size: 1765 32 564
Fragments arranged by size:
1765 564 32
Tth111I GACn'n_nGTC
Cuts at: 0 933 2361
Size: 933 1428
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 856 1350 1962 2361
Size: 856 494 612 399
Fragments arranged by size:
856 612 494 399
XhoI C'TCGA_G
Cuts at: 0 93 2361
Size: 93 2268
XmaI C'CCGG_G
Cuts at: 0 75 1300 2361
Size: 75 1225 1061
Fragments arranged by size:
1225 1061 75
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbvCI BcgI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssSI BstAPI BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NspI PasI PflMI PfoI
PpuMI PshAI PspOMI PspXI PsrI PstI PvuII SacI
SanDI SapI SexAI SfcI SfiI SfoI SmaI SmlI
SphI StuI StyI TatI TstI Tth111I XcmI XhoI
XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BciVI
BclI BfrBI BmtI BpuEI BsaAI BsaBI BsiWI BsmBI
BsrDI BsrGI BssHII BstBI BstEII ClaI CspCI DraI
DrdI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PsiI PvuI RsrII SacII SalI SbfI ScaI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII
XbaI XmnI ZraI