(Circular) (Six-base) MAPSORT of: pFN21AE6430.seq Check: 6345 from: 1 to: 6547 pFN21AE6430 6547 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3890 1937 Size: 1953 4594 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 3846 4674 5047 1563 Size: 2283 828 373 3063 Fragments arranged by size: 3063 2283 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3101 4610 5994 6262 1632 Size: 1469 1509 1384 268 1917 Fragments arranged by size: 1917 1509 1469 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 3050 5446 6423 1203 Size: 1847 2396 977 1327 Fragments arranged by size: 2396 1847 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 5276 5276 Size: 6547 AleI CACnn'nnGTG Cuts at: 1166 3635 1166 Size: 2469 4078 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6547 AlwNI CAG_nnn'CTG Cuts at: 3124 4290 5862 3124 Size: 1166 1572 3809 Fragments arranged by size: 3809 1572 1166 ApaLI G'TGCA_C Cuts at: 4604 5760 4604 Size: 1156 5391 ApoI r'AATT_y Cuts at: 1493 2999 3856 4082 4234 6343 1493 Size: 1506 857 226 152 2109 1697 Fragments arranged by size: 2109 1697 1506 857 226 152 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 3401 3759 3872 1870 Size: 73 1458 358 113 4545 Fragments arranged by size: 4545 1458 358 113 73 BamHI G'GATC_C Cuts at: 3418 3877 4244 3418 Size: 459 367 5721 Fragments arranged by size: 5721 459 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3515 3868 5323 618 Size: 325 217 627 1728 353 1455 1842 Fragments arranged by size: 1842 1728 1455 627 353 325 217 BanII G_rGCy'C Cuts at: 1391 3183 3866 1391 Size: 1792 683 4072 Fragments arranged by size: 4072 1792 683 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5576 BbvCI CC'TCA_GC Cuts at: 2316 2721 2316 Size: 405 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3260 3294 4738 4772 1903 Size: 34 1323 34 1444 34 3678 Fragments arranged by size: 3678 1444 1323 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4439 5649 4439 Size: 1210 5337 BclI T'GATC_A Cuts at: 1548 2877 1548 Size: 1329 5218 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5158 136 Size: 107 122 71 631 605 3486 1525 Fragments arranged by size: 3486 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6542 6542 Size: 6547 BlpI GC'TnA_GC Cuts at: 2325 3964 2325 Size: 1639 4908 Bme1580I G_kGCm'C Cuts at: 1790 4608 5764 1790 Size: 2818 1156 2573 Fragments arranged by size: 2818 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2713 1318 Size: 1395 5152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4278 5236 442 Size: 416 53 83 399 24 2861 958 1753 Fragments arranged by size: 2861 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2750 3146 3197 3857 5207 1239 Size: 971 540 396 51 660 1350 2579 Fragments arranged by size: 2579 1350 971 660 540 396 51 Bpu10I CC'TnA_GC Cuts at: 2316 2568 2721 2316 Size: 252 153 6142 Fragments arranged by size: 6142 252 153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3631 4672 5537 5835 3631 Size: 1041 865 298 4343 Fragments arranged by size: 4343 1041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 3105 5210 882 Size: 617 657 949 2105 2219 Fragments arranged by size: 2219 2105 949 657 617 BsaAI yAC'GTr Cuts at: 493 1204 4338 493 Size: 711 3134 2702 Fragments arranged by size: 3134 2702 711 BsaBI GATnn'nnATC Cuts at: 6541 6541 Size: 6547 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4980 5378 5652 5799 6210 4980 Size: 398 274 147 411 5317 Fragments arranged by size: 5317 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 2816 2846 1178 Size: 30 165 30 786 30 597 30 4879 Fragments arranged by size: 4879 786 597 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 1186 Size: 1021 5526 BseYI C'CCAG_C Cuts at: 2510 2793 5750 6246 2510 Size: 283 2957 496 2811 Fragments arranged by size: 2957 2811 496 283 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2962 1163 Size: 573 1226 4748 Fragments arranged by size: 4748 1226 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3997 4758 5786 1540 Size: 275 186 1996 761 1028 2301 Fragments arranged by size: 2301 1996 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3183 3402 4608 4693 5764 3183 Size: 219 1206 85 1071 3966 Fragments arranged by size: 3966 1206 1071 219 85 BsmI GAATG_Cn' Cuts at: 2401 3211 3224 3558 4058 4151 2401 Size: 810 13 334 500 93 4797 Fragments arranged by size: 4797 810 500 334 93 13 BsmBI CGTCTCn'nnnn_ Cuts at: 2472 2914 2985 3456 2472 Size: 442 71 471 5563 Fragments arranged by size: 5563 471 442 71 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3183 3402 3866 4608 4693 5764 1391 Size: 399 1393 219 464 742 85 1071 2174 Fragments arranged by size: 2174 1393 1071 742 464 399 219 85 BspEI T'CCGG_A Cuts at: 6210 6210 Size: 6547 BspHI T'CATG_A Cuts at: 4436 6166 4436 Size: 1730 4817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3486 3902 844 Size: 2642 416 3489 Fragments arranged by size: 3489 2642 416 BsrBI CCG'CTC Cuts at: 1394 1823 4434 1394 Size: 429 2611 3507 Fragments arranged by size: 3507 2611 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5042 5216 65 Size: 1183 3794 174 1396 Fragments arranged by size: 3794 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5191 5378 1700 Size: 110 3381 187 2869 Fragments arranged by size: 3381 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3053 96 Size: 2957 3590 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6547 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6547 BstYI r'GATC_y Cuts at: 1972 2761 3418 3577 3877 4244 4629 4646 6098 Size: 789 657 159 300 367 385 17 1452 Cuts at: 6098 6184 6196 6542 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 789 657 385 367 346 300 159 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3687 513 Size: 552 2622 3373 Fragments arranged by size: 3373 2622 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5808 Fragments arranged by size: 5808 683 56 BtsI GCAGTG_nn' Cuts at: 3490 4063 4857 4877 3490 Size: 573 794 20 5160 Fragments arranged by size: 5160 794 573 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6512 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DraIII CAC_nnn'GTG Cuts at: 2077 2966 2077 Size: 889 5658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3253 3994 4883 Size: 54 1425 50 275 18 1423 741 889 Cuts at: 4883 6512 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1423 889 741 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4477 1353 Size: 3124 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2294 3391 5134 5508 5654 387 Size: 1064 138 705 1097 1743 374 146 1280 Fragments arranged by size: 1743 1280 1097 1064 705 374 146 138 EcoICRI GAG'CTC Cuts at: 3181 3181 Size: 6547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2750 3101 3146 3197 3857 4610 Size: 393 578 540 351 45 51 660 753 Cuts at: 4610 5207 5994 6262 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 753 660 597 578 540 393 351 268 51 45 EcoO109I rG'GnC_Cy Cuts at: 3412 3498 3541 3811 3831 3412 Size: 86 43 270 20 6128 Fragments arranged by size: 6128 270 86 43 20 EcoRI G'AATT_C Cuts at: 2999 3856 4234 2999 Size: 857 378 5312 Fragments arranged by size: 5312 857 378 EcoRV GAT'ATC Cuts at: 2674 2674 Size: 6547 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2851 2851 Size: 6547 FspI TGC'GCA Cuts at: 3236 5053 3236 Size: 1817 4730 HaeII r_GCGC'y Cuts at: 1678 5694 1678 Size: 4016 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3410 3442 3716 3748 5188 Size: 32 135 32 1838 32 274 32 1440 Cuts at: 5188 5220 5262 5294 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1838 1440 274 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3429 3891 4137 669 Size: 988 281 1491 462 246 3079 Fragments arranged by size: 3079 1491 988 462 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2086 5661 5845 1305 Size: 781 3575 184 2007 Fragments arranged by size: 3575 2007 781 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3255 10 Size: 54 1425 343 1423 3302 Fragments arranged by size: 3302 1425 1423 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3635 4505 4864 5023 518 Size: 648 2469 870 359 159 2042 Fragments arranged by size: 2469 2042 870 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3963 4640 5788 6033 1606 Size: 2357 677 1148 245 2120 Fragments arranged by size: 2357 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3687 513 Size: 552 2622 3373 Fragments arranged by size: 3373 2622 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 3054 3905 5450 1132 Size: 97 1825 851 1545 2229 Fragments arranged by size: 2229 1825 1545 851 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6547 PciI A'CATG_T Cuts at: 3050 5446 3050 Size: 2396 4151 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 3804 1108 Size: 1515 1181 3851 Fragments arranged by size: 3851 1515 1181 PfoI T'CCnGG_A Cuts at: 3414 3500 3414 Size: 86 6461 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3412 3498 3541 3811 3831 3412 Size: 86 43 270 20 6128 Fragments arranged by size: 6128 270 86 43 20 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6547 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspXI vC'TCGA_Gb Cuts at: 3401 3401 Size: 6547 PstI C_TGCA'G Cuts at: 830 2917 3497 3550 3899 830 Size: 2087 580 53 349 3478 Fragments arranged by size: 3478 2087 580 349 53 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 SacI G_AGCT'C Cuts at: 3183 3183 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SanDI GG'GwC_CC Cuts at: 3541 3541 Size: 6547 SbfI CC_TGCA'GG Cuts at: 3550 3899 3550 Size: 349 6198 ScaI AGT'ACT Cuts at: 2834 4795 6507 2834 Size: 1961 1712 2874 Fragments arranged by size: 2874 1961 1712 SexAI A'CCwGG_T Cuts at: 2616 2616 Size: 6547 SfcI C'TryA_G Cuts at: 826 1045 2913 3493 3546 3895 5030 5711 5902 Size: 219 1868 580 53 349 1135 681 191 Cuts at: 5902 826 Size: 1471 Fragments arranged by size: 1868 1471 1135 681 580 349 219 191 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3761 3874 1872 Size: 1889 113 4545 Fragments arranged by size: 4545 1889 113 SmlI C'TyrA_G Cuts at: 820 1017 1943 3401 3610 4651 5552 5814 820 Size: 197 926 1458 209 1041 901 262 1553 Fragments arranged by size: 1553 1458 1041 926 901 262 209 197 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SspI AAT'ATT Cuts at: 5 52 2442 2464 4471 5 Size: 47 2390 22 2007 2081 Fragments arranged by size: 2390 2081 2007 47 22 StuI AGG'CCT Cuts at: 1517 2490 1517 Size: 973 5574 StyI C'CwwG_G Cuts at: 513 1065 3348 3687 3986 513 Size: 552 2283 339 299 3074 Fragments arranged by size: 3074 2283 552 339 299 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4574 4591 4744 4929 5374 325 Size: 1255 2994 17 153 185 445 1498 Fragments arranged by size: 2994 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2832 2899 3053 Size: 274 80 33 51 1443 855 67 154 Cuts at: 3053 4793 6505 96 Size: 1740 1712 138 Fragments arranged by size: 1740 1712 1443 855 274 154 138 80 67 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2121 2153 2753 2785 3482 3514 6507 6539 2121 Size: 32 600 32 697 32 2993 32 2129 Fragments arranged by size: 2993 2129 697 600 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2243 2609 2711 1316 Size: 927 366 102 5152 Fragments arranged by size: 5152 927 366 102 XbaI T'CTAG_A Cuts at: 3367 3883 3367 Size: 516 6031 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3685 3685 Size: 6547 XhoI C'TCGA_G Cuts at: 1943 3401 1943 Size: 1458 5089 XmaI C'CCGG_G Cuts at: 1870 3759 3872 1870 Size: 1889 113 4545 Fragments arranged by size: 4545 1889 113 XmnI GAAnn'nnTTC Cuts at: 3645 4676 3645 Size: 1031 5516 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacII SapI SfiI SfoI SrfI SwaI