(Circular) (Six-base) MAPSORT of: pFN21AE1124.seq Check: 3591 from: 1 to: 6112 pFN21AE1124 6112 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3034 3034 Size: 6112 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3922 278 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3455 1937 Size: 1518 4594 Acc65I G'GTAC_C Cuts at: 2823 3433 2823 Size: 610 5502 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2931 4175 5559 5827 1632 Size: 1299 1244 1384 268 1917 Fragments arranged by size: 1917 1384 1299 1244 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 5011 5988 1203 Size: 3808 977 1327 Fragments arranged by size: 3808 1327 977 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 2470 4841 2470 Size: 2371 3741 AleI CACnn'nnGTG Cuts at: 1166 2396 1166 Size: 1230 4882 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2375 1184 Size: 1191 4921 AlwNI CAG_nnn'CTG Cuts at: 2893 3016 3202 3855 5427 2893 Size: 123 186 653 1572 3578 Fragments arranged by size: 3578 1572 653 186 123 ApaLI G'TGCA_C Cuts at: 3210 4169 5325 3210 Size: 959 1156 3997 Fragments arranged by size: 3997 1156 959 ApoI r'AATT_y Cuts at: 1493 2798 3421 3647 3799 5908 1493 Size: 1305 623 226 152 2109 1697 Fragments arranged by size: 2109 1697 1305 623 226 152 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2333 2585 2722 3386 3437 1870 Size: 73 390 252 137 664 51 4545 Fragments arranged by size: 4545 664 390 252 137 73 51 BamHI G'GATC_C Cuts at: 2152 3442 3809 2152 Size: 1290 367 4455 Fragments arranged by size: 4455 1290 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2407 2558 2823 3118 3310 Size: 325 217 627 620 151 265 295 192 Cuts at: 3310 3433 4888 618 Size: 123 1455 1842 Fragments arranged by size: 1842 1455 627 620 325 295 265 217 192 151 123 BanII G_rGCy'C Cuts at: 1391 2063 2988 3431 1391 Size: 672 925 443 4072 Fragments arranged by size: 4072 925 672 443 BbeI G_GCGC'C Cuts at: 3314 3314 Size: 6112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2112 2427 3075 928 Size: 971 159 54 315 648 3965 Fragments arranged by size: 3965 971 648 315 159 54 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4303 4337 1903 Size: 34 2366 34 3678 Fragments arranged by size: 3678 2366 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 4723 136 Size: 107 122 71 631 605 568 2483 1525 Fragments arranged by size: 2483 1525 631 605 568 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 3214 3340 4173 5329 1790 Size: 1424 126 833 1156 2573 Fragments arranged by size: 2573 1424 1156 833 126 BmgBI CAC'GTC Cuts at: 1318 2349 1318 Size: 1031 5081 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2066 3843 4801 Size: 416 53 83 399 24 649 1777 958 Cuts at: 4801 442 Size: 1753 Fragments arranged by size: 1777 1753 958 649 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6112 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2467 2467 Size: 6112 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 3152 3257 4772 1239 Size: 1019 894 105 1515 2579 Fragments arranged by size: 2579 1515 1019 894 105 Bpu10I CC'TnA_GC Cuts at: 2057 2057 Size: 6112 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4237 5102 5400 4237 Size: 865 298 4949 Fragments arranged by size: 4949 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4775 882 Size: 617 3276 2219 Fragments arranged by size: 3276 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3181 3301 3903 493 Size: 711 1977 120 602 2702 Fragments arranged by size: 2702 1977 711 602 120 BsaBI GATnn'nnATC Cuts at: 6106 6106 Size: 6112 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2380 3311 3919 4301 Size: 53 83 186 936 847 931 608 382 Cuts at: 4301 4936 275 Size: 635 1451 Fragments arranged by size: 1451 936 931 847 635 608 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2778 4545 4943 5217 5364 5775 2778 Size: 1767 398 274 147 411 3115 Fragments arranged by size: 3115 1767 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2918 2948 2956 2986 1178 Size: 30 165 30 1515 30 8 30 4304 Fragments arranged by size: 4304 1515 165 30 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3223 1186 Size: 2037 4075 BseYI C'CCAG_C Cuts at: 2566 5315 5811 2566 Size: 2749 496 2867 Fragments arranged by size: 2867 2749 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2612 2723 3002 3068 3323 1163 Size: 573 876 111 279 66 255 3952 Fragments arranged by size: 3952 876 573 279 255 111 66 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3333 3348 3562 4323 5351 1540 Size: 275 186 1332 15 214 761 1028 2301 Fragments arranged by size: 2301 1332 1028 761 275 214 186 15 BsiHKAI G_wGCw'C Cuts at: 2966 2988 3214 3393 4173 4258 5329 2966 Size: 22 226 179 780 85 1071 3749 Fragments arranged by size: 3749 1071 780 226 179 85 22 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2063 2966 2988 3214 3340 3393 3431 Size: 399 273 903 22 226 126 53 38 Cuts at: 3431 4173 4258 5329 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 903 742 399 273 226 126 85 53 38 22 BspEI T'CCGG_A Cuts at: 5775 5775 Size: 6112 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2122 3034 3467 844 Size: 1278 912 433 3489 Fragments arranged by size: 3489 1278 912 433 BsrBI CCG'CTC Cuts at: 1394 1823 2448 2761 3999 1394 Size: 429 625 313 1238 3507 Fragments arranged by size: 3507 1238 625 429 313 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2321 4756 4943 1700 Size: 110 511 2435 187 2869 Fragments arranged by size: 2869 2435 511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6112 BssSI C'ACGA_G Cuts at: 2432 4172 5184 2432 Size: 1740 1012 3360 Fragments arranged by size: 3360 1740 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2643 3016 794 Size: 1849 373 3890 Fragments arranged by size: 3890 1849 373 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2152 2272 3051 3171 3442 3809 4194 4211 Size: 180 120 779 120 271 367 385 17 Cuts at: 4211 5663 5749 5761 6107 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 779 385 367 346 271 180 120 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2313 3225 3243 513 Size: 552 1248 912 18 3382 Fragments arranged by size: 3382 1248 912 552 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2377 3094 487 Size: 683 56 1151 717 3505 Fragments arranged by size: 3505 1151 717 683 56 BtsI GCAGTG_nn' Cuts at: 2052 3628 4422 4442 2052 Size: 1576 794 20 3722 Fragments arranged by size: 3722 1576 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6077 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DraIII CAC_nnn'GTG Cuts at: 2865 2865 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2319 3030 Size: 54 1425 50 275 18 195 294 711 Cuts at: 3030 3330 3345 3559 4448 6077 8 Size: 300 15 214 889 1629 43 Fragments arranged by size: 1629 1425 889 711 300 294 275 214 195 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3330 3345 3559 1537 Size: 275 1518 15 214 4090 Fragments arranged by size: 4090 1518 275 214 15 EarI CTCTTCn'nnn_ Cuts at: 1353 2535 2693 4042 1353 Size: 1182 158 1349 3423 Fragments arranged by size: 3423 1349 1182 158 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2986 2986 Size: 6112 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2258 2931 3152 3257 4175 4772 5559 Size: 393 626 673 221 105 918 597 787 Cuts at: 5559 5827 1239 Size: 268 1524 Fragments arranged by size: 1524 918 787 673 626 597 393 268 221 105 EcoO109I rG'GnC_Cy Cuts at: 2308 3013 2308 Size: 705 5407 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2669 3314 5259 1678 Size: 991 645 1945 2531 Fragments arranged by size: 2531 1945 991 645 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2124 2156 2456 2488 4753 Size: 32 135 32 552 32 300 32 2265 Cuts at: 4753 4785 4827 4859 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2265 552 300 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KasI G'GCGC_C Cuts at: 3310 3310 Size: 6112 KpnI G_GTAC'C Cuts at: 2827 3437 2827 Size: 610 5502 MfeI C'AATT_G Cuts at: 2143 3711 2143 Size: 1568 4544 MluI A'CGCG_T Cuts at: 5988 5988 Size: 6112 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 2187 2643 2739 2841 5226 5410 1305 Size: 687 195 456 96 102 2385 184 2007 Fragments arranged by size: 2385 2007 687 456 195 184 102 96 MscI TGG'CCA Cuts at: 10 64 1489 1832 3032 10 Size: 54 1425 343 1200 3090 Fragments arranged by size: 3090 1425 1200 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2396 2399 4070 4429 4588 518 Size: 648 1230 3 1671 359 159 2042 Fragments arranged by size: 2042 1671 1230 648 359 159 3 MspA1I CmG'CkG Cuts at: 1606 2478 2752 2881 3217 3245 3307 3528 4205 Size: 872 274 129 336 28 62 221 677 Cuts at: 4205 5353 5598 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 872 677 336 274 245 221 129 62 28 NaeI GCC'GGC Cuts at: 2323 2323 Size: 6112 NarI GG'CG_CC Cuts at: 3311 3311 Size: 6112 NcoI C'CATG_G Cuts at: 513 1065 2313 513 Size: 552 1248 4312 Fragments arranged by size: 4312 1248 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NgoMIV G'CCGG_C Cuts at: 2321 2321 Size: 6112 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6112 NotI GC'GGCC_GC Cuts at: 3559 3559 Size: 6112 NspI r_CATG'y Cuts at: 1132 1229 2626 3470 5015 1132 Size: 97 1397 844 1545 2229 Fragments arranged by size: 2229 1545 1397 844 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6112 PciI A'CATG_T Cuts at: 5011 5011 Size: 6112 PflMI CCAn_nnn'nTGG Cuts at: 1108 2893 3181 1108 Size: 1785 288 4039 Fragments arranged by size: 4039 1785 288 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PmlI CAC'GTG Cuts at: 3181 3181 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2308 2308 Size: 6112 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PspXI vC'TCGA_Gb Cuts at: 3386 3386 Size: 6112 PstI C_TGCA'G Cuts at: 830 3207 3360 3464 830 Size: 2377 153 104 3478 Fragments arranged by size: 3478 2377 153 104 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2478 2752 3217 3307 2478 Size: 274 465 90 5283 Fragments arranged by size: 5283 465 274 90 SacI G_AGCT'C Cuts at: 2988 2988 Size: 6112 SacII CC_GC'GG Cuts at: 3246 3246 Size: 6112 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 3203 3356 3368 3460 4595 5276 5467 Size: 219 2158 153 12 92 1135 681 191 Cuts at: 5467 826 Size: 1471 Fragments arranged by size: 2158 1471 1135 681 219 191 153 92 12 SfoI GGC'GCC Cuts at: 3312 3312 Size: 6112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 3439 1872 Size: 1567 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3386 4216 5117 5379 820 Size: 197 926 1443 830 901 262 1553 Fragments arranged by size: 1553 1443 926 901 830 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 3470 1132 Size: 2338 3774 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2313 3551 513 Size: 552 1248 1238 3074 Fragments arranged by size: 3074 1248 1238 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2085 4139 4156 4309 4494 4939 325 Size: 1255 505 2054 17 153 185 445 1498 Fragments arranged by size: 2054 1498 1255 505 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4358 6070 96 Size: 274 80 33 51 1443 2381 1712 138 Fragments arranged by size: 2381 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2956 2988 6072 6104 2956 Size: 32 3084 32 2964 Fragments arranged by size: 3084 2964 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 3386 1943 Size: 1443 4669 XmaI C'CCGG_G Cuts at: 1870 3437 1870 Size: 1567 4545 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3920 276 Size: 53 83 186 936 2386 2468 Fragments arranged by size: 2468 2386 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PsrI RsrII SanDI SapI SexAI SfiI SrfI SwaI XcmI