(Linear) (Six-base) MAPSORT of: KIEE1124.seq Check: 2045 from: 1 to: 1407 KIEE1124.orf With 182 enzymes: SgfI * July 15, 2010 18:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1029 1407 Size: 1029 378 Acc65I G'GTAC_C Cuts at: 0 818 1407 Size: 818 589 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 926 1407 Size: 926 481 AhdI GACnn_n'nnGTC Cuts at: 0 465 1407 Size: 465 942 AleI CACnn'nnGTG Cuts at: 0 391 1407 Size: 391 1016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 370 1407 Size: 370 1037 AlwNI CAG_nnn'CTG Cuts at: 0 888 1011 1197 1407 Size: 888 123 186 210 Fragments arranged by size: 888 210 186 123 ApaLI G'TGCA_C Cuts at: 0 1205 1407 Size: 1205 202 ApoI r'AATT_y Cuts at: 0 793 1407 Size: 793 614 AvaI C'yCGr_G Cuts at: 0 328 580 717 1381 1407 Size: 328 252 137 664 26 Fragments arranged by size: 664 328 252 137 26 BamHI G'GATC_C Cuts at: 0 147 1407 Size: 147 1260 BanI G'GyrC_C Cuts at: 0 402 553 818 1113 1305 1407 Size: 402 151 265 295 192 102 Fragments arranged by size: 402 295 265 192 151 102 BanII G_rGCy'C Cuts at: 0 58 983 1407 Size: 58 925 424 Fragments arranged by size: 925 424 58 BbeI G_GCGC'C Cuts at: 0 1309 1407 Size: 1309 98 BbsI GAAGACnn'nnnn_ Cuts at: 0 53 107 422 1070 1407 Size: 53 54 315 648 337 Fragments arranged by size: 648 337 315 54 53 BglI GCCn_nnn'nGGC Cuts at: 0 235 1407 Size: 235 1172 Bme1580I G_kGCm'C Cuts at: 0 1209 1335 1407 Size: 1209 126 72 Fragments arranged by size: 1209 126 72 BmgBI CAC'GTC Cuts at: 0 344 1407 Size: 344 1063 BmrI ACTGGGnnnn_n' Cuts at: 0 61 1407 Size: 61 1346 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 462 1407 Size: 462 945 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 1147 1252 1407 Size: 253 894 105 155 Fragments arranged by size: 894 253 155 105 Bpu10I CC'TnA_GC Cuts at: 0 52 1407 Size: 52 1355 BsaAI yAC'GTr Cuts at: 0 1176 1296 1407 Size: 1176 120 111 Fragments arranged by size: 1176 120 111 BsaHI Gr'CG_yC Cuts at: 0 375 1306 1407 Size: 375 931 101 Fragments arranged by size: 931 375 101 BsaWI w'CCGG_w Cuts at: 0 773 1407 Size: 773 634 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 913 943 951 981 1407 Size: 913 30 8 30 426 Fragments arranged by size: 913 426 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1218 1407 Size: 1218 189 BseYI C'CCAG_C Cuts at: 0 561 1407 Size: 561 846 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 718 997 1063 1318 1407 Size: 607 111 279 66 255 89 Fragments arranged by size: 607 279 255 111 89 66 BsiEI CG_ry'CG Cuts at: 0 1328 1343 1407 Size: 1328 15 64 Fragments arranged by size: 1328 64 15 BsiHKAI G_wGCw'C Cuts at: 0 961 983 1209 1388 1407 Size: 961 22 226 179 19 Fragments arranged by size: 961 226 179 22 19 Bsp1286I G_dGCh'C Cuts at: 0 58 961 983 1209 1335 1388 1407 Size: 58 903 22 226 126 53 19 Fragments arranged by size: 903 226 126 58 53 22 19 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 117 1029 1407 Size: 117 912 378 Fragments arranged by size: 912 378 117 BsrBI CCG'CTC Cuts at: 0 443 756 1407 Size: 443 313 651 Fragments arranged by size: 651 443 313 BsrFI r'CCGG_y Cuts at: 0 316 1407 Size: 316 1091 BssSI C'ACGA_G Cuts at: 0 427 1407 Size: 427 980 BstAPI GCAn_nnn'nTGC Cuts at: 0 638 1011 1407 Size: 638 373 396 Fragments arranged by size: 638 396 373 BstYI r'GATC_y Cuts at: 0 147 267 1046 1166 1407 Size: 147 120 779 120 241 Fragments arranged by size: 779 241 147 120 120 BtgI C'CryG_G Cuts at: 0 308 1220 1238 1407 Size: 308 912 18 169 Fragments arranged by size: 912 308 169 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 372 1089 1407 Size: 372 717 318 Fragments arranged by size: 717 372 318 BtsI GCAGTG_nn' Cuts at: 0 47 1407 Size: 47 1360 DraIII CAC_nnn'GTG Cuts at: 0 860 1407 Size: 860 547 EaeI y'GGCC_r Cuts at: 0 20 314 1025 1325 1340 1407 Size: 20 294 711 300 15 67 Fragments arranged by size: 711 300 294 67 20 15 EagI C'GGCC_G Cuts at: 0 1325 1340 1407 Size: 1325 15 67 Fragments arranged by size: 1325 67 15 EarI CTCTTCn'nnn_ Cuts at: 0 530 688 1407 Size: 530 158 719 Fragments arranged by size: 719 530 158 EcoICRI GAG'CTC Cuts at: 0 981 1407 Size: 981 426 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 926 1147 1252 1407 Size: 253 673 221 105 155 Fragments arranged by size: 673 253 221 155 105 EcoO109I rG'GnC_Cy Cuts at: 0 303 1008 1407 Size: 303 705 399 Fragments arranged by size: 705 399 303 HaeII r_GCGC'y Cuts at: 0 664 1309 1407 Size: 664 645 98 Fragments arranged by size: 664 645 98 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 119 151 451 483 1407 Size: 119 32 300 32 924 Fragments arranged by size: 924 300 119 32 32 KasI G'GCGC_C Cuts at: 0 1305 1407 Size: 1305 102 KpnI G_GTAC'C Cuts at: 0 822 1407 Size: 822 585 MfeI C'AATT_G Cuts at: 0 138 1407 Size: 138 1269 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 182 638 734 836 1407 Size: 182 456 96 102 571 Fragments arranged by size: 571 456 182 102 96 MscI TGG'CCA Cuts at: 0 1027 1407 Size: 1027 380 MslI CAynn'nnrTG Cuts at: 0 391 394 1407 Size: 391 3 1013 Fragments arranged by size: 1013 391 3 MspA1I CmG'CkG Cuts at: 0 473 747 876 1212 1240 1302 1407 Size: 473 274 129 336 28 62 105 Fragments arranged by size: 473 336 274 129 105 62 28 NaeI GCC'GGC Cuts at: 0 318 1407 Size: 318 1089 NarI GG'CG_CC Cuts at: 0 1306 1407 Size: 1306 101 NcoI C'CATG_G Cuts at: 0 308 1407 Size: 308 1099 NgoMIV G'CCGG_C Cuts at: 0 316 1407 Size: 316 1091 NspI r_CATG'y Cuts at: 0 621 1407 Size: 621 786 PflMI CCAn_nnn'nTGG Cuts at: 0 888 1176 1407 Size: 888 288 231 Fragments arranged by size: 888 288 231 PmlI CAC'GTG Cuts at: 0 1176 1407 Size: 1176 231 PpuMI rG'GwC_Cy Cuts at: 0 303 1407 Size: 303 1104 PspXI vC'TCGA_Gb Cuts at: 0 1381 1407 Size: 1381 26 PstI C_TGCA'G Cuts at: 0 1202 1355 1407 Size: 1202 153 52 Fragments arranged by size: 1202 153 52 PvuII CAG'CTG Cuts at: 0 473 747 1212 1302 1407 Size: 473 274 465 90 105 Fragments arranged by size: 473 465 274 105 90 SacI G_AGCT'C Cuts at: 0 983 1407 Size: 983 424 SacII CC_GC'GG Cuts at: 0 1241 1407 Size: 1241 166 SfcI C'TryA_G Cuts at: 0 1198 1351 1363 1407 Size: 1198 153 12 44 Fragments arranged by size: 1198 153 44 12 SfoI GGC'GCC Cuts at: 0 1307 1407 Size: 1307 100 SmlI C'TyrA_G Cuts at: 0 1381 1407 Size: 1381 26 StyI C'CwwG_G Cuts at: 0 308 1407 Size: 308 1099 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 80 1407 Size: 80 1327 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 951 983 1407 Size: 951 32 424 Fragments arranged by size: 951 424 32 XhoI C'TCGA_G Cuts at: 0 1381 1407 Size: 1381 26 Enzymes that do cut: AarI Acc65I AcuI AhdI AleI AlfI AlwNI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstYI BtgI BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI PmlI PpuMI PspXI PstI PvuII SacI SacII SfcI SfoI SmlI StyI TaqII TstI XhoI Enzymes that do not cut: AatII AccI AclI AfeI AflII AflIII AgeI AloI ApaI AscI AseI AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI BpuEI BsaI BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MluI NdeI NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XbaI XcmI XmaI XmnI ZraI