(Circular) (Six-base) MAPSORT of: pFN21AE1436.seq Check: 7467 from: 1 to: 5464 pFN21AE1436 5464 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2495 2495 Size: 5464 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2807 1937 Size: 870 4594 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3527 4911 5179 1632 Size: 1895 1384 268 1917 Fragments arranged by size: 1917 1895 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 2630 4295 2630 Size: 1665 3799 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5464 AlwNI CAG_nnn'CTG Cuts at: 3207 4779 3207 Size: 1572 3892 ApaI G_GGCC'C Cuts at: 2106 2285 2294 2314 2106 Size: 179 9 20 5256 Fragments arranged by size: 5256 179 20 9 ApaLI G'TGCA_C Cuts at: 2574 3521 4677 2574 Size: 947 1156 3361 Fragments arranged by size: 3361 1156 947 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2125 2532 2789 1870 Size: 73 182 407 257 4545 Fragments arranged by size: 4545 407 257 182 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2494 2714 2785 4240 618 Size: 325 217 627 707 220 71 1455 1842 Fragments arranged by size: 1842 1455 707 627 325 220 217 71 BanII G_rGCy'C Cuts at: 1391 2106 2285 2294 2314 2323 2469 2783 1391 Size: 715 179 9 20 9 146 314 4072 Fragments arranged by size: 4072 715 314 179 146 20 9 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2662 928 Size: 971 763 3730 Fragments arranged by size: 3730 971 763 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3655 3689 1903 Size: 34 1718 34 3678 Fragments arranged by size: 3678 1718 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2141 3356 4566 2141 Size: 1215 1210 3039 Fragments arranged by size: 3039 1215 1210 BclI T'GATC_A Cuts at: 1548 2439 1548 Size: 891 4573 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2154 2280 2289 Size: 107 122 71 631 605 482 126 9 Cuts at: 2289 4075 136 Size: 1786 1525 Fragments arranged by size: 1786 1525 631 605 482 126 122 107 71 9 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2090 2881 2090 Size: 791 4673 Bme1580I G_kGCm'C Cuts at: 1790 2106 2285 2294 2314 2499 2578 3525 4681 Size: 316 179 9 20 185 79 947 1156 Cuts at: 4681 1790 Size: 2573 Fragments arranged by size: 2573 1156 947 316 185 179 79 20 9 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2030 2711 3195 Size: 416 53 83 399 24 613 681 484 Cuts at: 3195 4153 442 Size: 958 1753 Fragments arranged by size: 1753 958 681 613 484 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2229 2337 2672 4124 1239 Size: 990 108 335 1452 2579 Fragments arranged by size: 2579 1452 990 335 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2630 3589 4454 4752 2630 Size: 959 865 298 3342 Fragments arranged by size: 3342 959 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2205 2232 2507 4127 882 Size: 617 706 27 275 1620 2219 Fragments arranged by size: 2219 1620 706 617 275 27 BsaAI yAC'GTr Cuts at: 493 1204 2437 3255 493 Size: 711 1233 818 2702 Fragments arranged by size: 2702 1233 818 711 BsaBI GATnn'nnATC Cuts at: 2015 5458 2015 Size: 3443 2021 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2630 3897 4295 4569 4716 5127 2630 Size: 1267 398 274 147 411 2967 Fragments arranged by size: 2967 1267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5464 BseYI C'CCAG_C Cuts at: 2085 2107 4667 5163 2085 Size: 22 2560 496 2386 Fragments arranged by size: 2560 2386 496 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2080 1163 Size: 573 344 4547 Fragments arranged by size: 4547 573 344 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2578 3525 3610 4681 2578 Size: 947 85 1071 3361 Fragments arranged by size: 3361 1071 947 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2285 2294 2314 2323 2469 2499 Size: 399 316 179 9 20 9 146 30 Cuts at: 2499 2578 2783 3525 3610 4681 1391 Size: 79 205 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 316 205 179 146 85 79 30 20 9 9 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2495 2819 844 Size: 1651 324 3489 Fragments arranged by size: 3489 1651 324 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3959 4133 65 Size: 1183 2711 174 1396 Fragments arranged by size: 2711 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2630 4108 4295 1700 Size: 110 820 1478 187 2869 Fragments arranged by size: 2869 1478 820 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 2587 2623 1184 Size: 1403 36 4025 Fragments arranged by size: 4025 1403 36 BstYI r'GATC_y Cuts at: 1972 2016 2794 3161 3546 3563 5015 5101 5113 Size: 44 778 367 385 17 1452 86 12 Cuts at: 5113 5459 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 778 385 367 346 86 44 17 12 BtgI C'CryG_G Cuts at: 513 1065 2021 2215 2286 513 Size: 552 956 194 71 3691 Fragments arranged by size: 3691 956 552 194 71 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2479 2980 3774 3794 2479 Size: 501 794 20 4149 Fragments arranged by size: 4149 794 501 20 ClaI AT'CG_AT Cuts at: 1554 2070 1554 Size: 516 4948 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2155 2563 2911 Size: 54 1425 50 275 18 325 408 348 Cuts at: 2911 3800 5429 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 408 348 325 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3394 1353 Size: 2041 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2229 2337 2672 3527 4124 4911 5179 Size: 393 597 108 335 855 597 787 268 Cuts at: 5179 1239 Size: 1524 Fragments arranged by size: 1524 855 787 597 597 393 335 268 108 EcoO109I rG'GnC_Cy Cuts at: 2103 2166 2281 2282 2291 2310 2311 2327 2103 Size: 63 115 1 9 19 1 16 5240 Fragments arranged by size: 5240 115 63 19 16 9 1 1 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 EcoRV GAT'ATC Cuts at: 2074 2074 Size: 5464 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 4611 1678 Size: 2933 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2678 2710 4105 4137 4179 Size: 32 135 32 1106 32 1395 32 42 Cuts at: 4179 4211 1373 Size: 32 2626 Fragments arranged by size: 2626 1395 1106 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4578 4762 1305 Size: 3273 184 2007 Fragments arranged by size: 3273 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2157 10 Size: 54 1425 343 325 3317 Fragments arranged by size: 3317 1425 343 325 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2447 3422 3781 3940 518 Size: 648 843 438 975 359 159 2042 Fragments arranged by size: 2042 975 843 648 438 359 159 MspA1I CmG'CkG Cuts at: 1606 2094 2880 3557 4705 4950 1606 Size: 488 786 677 1148 245 2120 Fragments arranged by size: 2120 1148 786 677 488 245 NcoI C'CATG_G Cuts at: 513 1065 2021 2215 2286 513 Size: 552 956 194 71 3691 Fragments arranged by size: 3691 956 552 194 71 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2822 4367 1132 Size: 97 1593 1545 2229 Fragments arranged by size: 2229 1593 1545 97 PasI CC'CwG_GG Cuts at: 1348 2135 2171 2243 2306 1348 Size: 787 36 72 63 4506 Fragments arranged by size: 4506 787 72 63 36 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5464 PfoI T'CCnGG_A Cuts at: 2260 2260 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PmlI CAC'GTG Cuts at: 2437 2437 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2166 2166 Size: 5464 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PspOMI G'GGCC_C Cuts at: 2102 2281 2290 2310 2102 Size: 179 9 20 5256 Fragments arranged by size: 5256 179 20 9 PstI C_TGCA'G Cuts at: 830 2679 2816 830 Size: 1849 137 3478 Fragments arranged by size: 3478 1849 137 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 PvuII CAG'CTG Cuts at: 2094 2094 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SanDI GG'GwC_CC Cuts at: 2166 2166 Size: 5464 SbfI CC_TGCA'GG Cuts at: 2679 2816 2679 Size: 137 5327 ScaI AGT'ACT Cuts at: 2040 3712 5424 2040 Size: 1672 1712 2080 Fragments arranged by size: 2080 1712 1672 SfcI C'TryA_G Cuts at: 826 1045 2675 2812 3947 4628 4819 826 Size: 219 1630 137 1135 681 191 1471 Fragments arranged by size: 1630 1471 1135 681 219 191 137 SfiI GGCCn_nnn'nGGCC Cuts at: 2154 2280 2289 2154 Size: 126 9 5329 Fragments arranged by size: 5329 126 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 2630 1700 Size: 930 4534 SmaI CCC'GGG Cuts at: 1872 2127 2791 1872 Size: 255 664 4545 Fragments arranged by size: 4545 664 255 SmlI C'TyrA_G Cuts at: 820 1017 1943 2532 2645 3568 4469 4731 820 Size: 197 926 589 113 923 901 262 1553 Fragments arranged by size: 1553 926 923 901 589 262 197 113 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2822 1132 Size: 1690 3774 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5464 StyI C'CwwG_G Cuts at: 513 1065 2021 2215 2277 2286 2903 513 Size: 552 956 194 62 9 617 3074 Fragments arranged by size: 3074 956 617 552 194 62 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2375 3491 3508 3661 3846 4291 325 Size: 1255 795 1116 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1116 795 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2038 3710 5422 Size: 274 80 33 51 1443 61 1672 1712 Cuts at: 5422 96 Size: 138 Fragments arranged by size: 1712 1672 1443 274 138 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2284 2635 2284 Size: 351 5113 XhoI C'TCGA_G Cuts at: 1943 2532 1943 Size: 589 4875 XmaI C'CCGG_G Cuts at: 1870 2125 2789 1870 Size: 255 664 4545 Fragments arranged by size: 4545 664 255 XmnI GAAnn'nnTTC Cuts at: 2669 3593 2669 Size: 924 4540 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SapI SexAI SfoI SrfI SwaI