(Linear) (Six-base) MAPSORT of: KIEE1436.seq Check: 9046 from: 1 to: 759 KIEE1436.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 490 759 Size: 490 269 AgeI A'CCGG_T Cuts at: 0 625 759 Size: 625 134 ApaI G_GGCC'C Cuts at: 0 101 280 289 309 759 Size: 101 179 9 20 450 Fragments arranged by size: 450 179 101 20 9 ApaLI G'TGCA_C Cuts at: 0 569 759 Size: 569 190 AvaI C'yCGr_G Cuts at: 0 120 527 759 Size: 120 407 232 Fragments arranged by size: 407 232 120 BanI G'GyrC_C Cuts at: 0 489 709 759 Size: 489 220 50 Fragments arranged by size: 489 220 50 BanII G_rGCy'C Cuts at: 0 101 280 289 309 318 464 759 Size: 101 179 9 20 9 146 295 Fragments arranged by size: 295 179 146 101 20 9 9 BbsI GAAGACnn'nnnn_ Cuts at: 0 657 759 Size: 657 102 BciVI GTATCCnnnnn_n' Cuts at: 0 136 759 Size: 136 623 BclI T'GATC_A Cuts at: 0 434 759 Size: 434 325 BglI GCCn_nnn'nGGC Cuts at: 0 149 275 284 759 Size: 149 126 9 475 Fragments arranged by size: 475 149 126 9 BlpI GC'TnA_GC Cuts at: 0 85 759 Size: 85 674 Bme1580I G_kGCm'C Cuts at: 0 101 280 289 309 494 573 759 Size: 101 179 9 20 185 79 186 Fragments arranged by size: 186 185 179 101 79 20 9 BmrI ACTGGGnnnn_n' Cuts at: 0 25 706 759 Size: 25 681 53 Fragments arranged by size: 681 53 25 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 332 667 759 Size: 224 108 335 92 Fragments arranged by size: 335 224 108 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 625 759 Size: 625 134 BsaI GGTCTCn'nnnn_ Cuts at: 0 200 227 502 759 Size: 200 27 275 257 Fragments arranged by size: 275 257 200 27 BsaAI yAC'GTr Cuts at: 0 432 759 Size: 432 327 BsaBI GATnn'nnATC Cuts at: 0 10 759 Size: 10 749 BsaWI w'CCGG_w Cuts at: 0 625 759 Size: 625 134 BseYI C'CCAG_C Cuts at: 0 80 102 759 Size: 80 22 657 Fragments arranged by size: 657 80 22 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 75 759 Size: 75 684 BsiHKAI G_wGCw'C Cuts at: 0 573 759 Size: 573 186 Bsp1286I G_dGCh'C Cuts at: 0 101 280 289 309 318 464 494 573 Size: 101 179 9 20 9 146 30 79 Cuts at: 573 759 Size: 186 Fragments arranged by size: 186 179 146 101 79 30 20 9 9 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 490 759 Size: 490 269 BsrFI r'CCGG_y Cuts at: 0 625 759 Size: 625 134 BstEII G'GTnAC_C Cuts at: 0 582 618 759 Size: 582 36 141 Fragments arranged by size: 582 141 36 BstYI r'GATC_y Cuts at: 0 11 759 Size: 11 748 BtgI C'CryG_G Cuts at: 0 16 210 281 759 Size: 16 194 71 478 Fragments arranged by size: 478 194 71 16 BtsI GCAGTG_nn' Cuts at: 0 474 759 Size: 474 285 ClaI AT'CG_AT Cuts at: 0 65 759 Size: 65 694 EaeI y'GGCC_r Cuts at: 0 150 558 759 Size: 150 408 201 Fragments arranged by size: 408 201 150 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 224 332 667 759 Size: 224 108 335 92 Fragments arranged by size: 335 224 108 92 EcoO109I rG'GnC_Cy Cuts at: 0 98 161 276 277 286 305 306 322 Size: 98 63 115 1 9 19 1 16 Cuts at: 322 759 Size: 437 Fragments arranged by size: 437 115 98 63 19 16 9 1 1 EcoRV GAT'ATC Cuts at: 0 69 759 Size: 69 690 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 673 705 759 Size: 673 32 54 Fragments arranged by size: 673 54 32 MscI TGG'CCA Cuts at: 0 152 759 Size: 152 607 MslI CAynn'nnrTG Cuts at: 0 442 759 Size: 442 317 MspA1I CmG'CkG Cuts at: 0 89 759 Size: 89 670 NcoI C'CATG_G Cuts at: 0 16 210 281 759 Size: 16 194 71 478 Fragments arranged by size: 478 194 71 16 PasI CC'CwG_GG Cuts at: 0 130 166 238 301 759 Size: 130 36 72 63 458 Fragments arranged by size: 458 130 72 63 36 PfoI T'CCnGG_A Cuts at: 0 255 759 Size: 255 504 PmlI CAC'GTG Cuts at: 0 432 759 Size: 432 327 PpuMI rG'GwC_Cy Cuts at: 0 161 759 Size: 161 598 PspOMI G'GGCC_C Cuts at: 0 97 276 285 305 759 Size: 97 179 9 20 454 Fragments arranged by size: 454 179 97 20 9 PstI C_TGCA'G Cuts at: 0 674 759 Size: 674 85 PvuII CAG'CTG Cuts at: 0 89 759 Size: 89 670 SanDI GG'GwC_CC Cuts at: 0 161 759 Size: 161 598 SbfI CC_TGCA'GG Cuts at: 0 674 759 Size: 674 85 ScaI AGT'ACT Cuts at: 0 35 759 Size: 35 724 SfcI C'TryA_G Cuts at: 0 670 759 Size: 670 89 SfiI GGCCn_nnn'nGGCC Cuts at: 0 149 275 284 759 Size: 149 126 9 475 Fragments arranged by size: 475 149 126 9 SgrAI Cr'CCGG_yG Cuts at: 0 625 759 Size: 625 134 SmaI CCC'GGG Cuts at: 0 122 759 Size: 122 637 SmlI C'TyrA_G Cuts at: 0 527 640 759 Size: 527 113 119 Fragments arranged by size: 527 119 113 StyI C'CwwG_G Cuts at: 0 16 210 272 281 759 Size: 16 194 62 9 478 Fragments arranged by size: 478 194 62 16 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 370 759 Size: 370 389 TatI w'GTAC_w Cuts at: 0 33 759 Size: 33 726 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 279 630 759 Size: 279 351 129 Fragments arranged by size: 351 279 129 XhoI C'TCGA_G Cuts at: 0 527 759 Size: 527 232 XmaI C'CCGG_G Cuts at: 0 120 759 Size: 120 639 XmnI GAAnn'nnTTC Cuts at: 0 664 759 Size: 664 95 Enzymes that do cut: AarI AgeI ApaI ApaLI AvaI BanI BanII BbsI BciVI BclI BglI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaBI BsaWI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BstEII BstYI BtgI BtsI ClaI EaeI Eco57MI EcoO109I EcoRV Hin4I MscI MslI MspA1I NcoI PasI PfoI PmlI PpuMI PspOMI PstI PvuII SanDI SbfI ScaI SfcI SfiI SgrAI SmaI SmlI StyI TaqII TatI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BfrBI BglII BmgBI BmtI BplI Bpu10I BsaHI BsaXI BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgZI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoNI EcoRI FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SapI SexAI SfoI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI ZraI