(Circular) (Six-base) MAPSORT of: pFN21AE9574.seq Check: 4533 from: 1 to: 5755 pFN21AE9574 5755 bp With 182 enzymes: SgfI * July 6, 2012 17:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 3076 3076 Size: 5755 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2997 3818 5202 5470 1632 Size: 1365 821 1384 268 1917 Fragments arranged by size: 1917 1384 1365 821 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2361 2967 4654 5631 1203 Size: 1158 606 1687 977 1327 Fragments arranged by size: 1687 1327 1158 977 606 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 2078 1166 Size: 912 4843 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2354 1184 Size: 1170 4585 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 3064 3290 3442 5551 1493 Size: 1571 226 152 2109 1697 Fragments arranged by size: 2109 1697 1571 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2433 3076 4531 618 Size: 325 217 627 646 643 1455 1842 Fragments arranged by size: 1842 1455 646 643 627 325 217 BanII G_rGCy'C Cuts at: 1391 3074 1391 Size: 1683 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2137 3047 928 Size: 971 238 910 3636 Fragments arranged by size: 3636 971 910 238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2050 3647 4857 2050 Size: 1597 1210 2948 Fragments arranged by size: 2948 1597 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2459 4366 136 Size: 107 122 71 631 605 787 1907 1525 Fragments arranged by size: 1907 1525 787 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2043 3816 4972 1790 Size: 253 1773 1156 2573 Fragments arranged by size: 2573 1773 1156 253 BmgBI CAC'GTC Cuts at: 1318 2286 2691 1318 Size: 968 405 4382 Fragments arranged by size: 4382 968 405 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5755 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2828 4415 1239 Size: 1589 1587 2579 Fragments arranged by size: 2579 1589 1587 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2711 3880 4745 5043 2711 Size: 1169 865 298 3423 Fragments arranged by size: 3423 1169 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4418 882 Size: 617 2919 2219 Fragments arranged by size: 2919 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2905 3546 493 Size: 711 1701 641 2702 Fragments arranged by size: 2702 1701 711 641 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2685 2961 4188 4586 4860 5007 5418 2685 Size: 276 1227 398 274 147 411 3022 Fragments arranged by size: 3022 1227 411 398 276 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2240 2270 2801 2831 2987 Size: 30 165 30 837 30 531 30 156 Cuts at: 2987 3017 1178 Size: 30 3916 Fragments arranged by size: 3916 837 531 165 156 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2555 2948 1186 Size: 1369 393 3993 Fragments arranged by size: 3993 1369 393 BseYI C'CCAG_C Cuts at: 2161 2951 4958 5454 2161 Size: 790 2007 496 2462 Fragments arranged by size: 2462 2007 790 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2404 3205 3966 4994 1540 Size: 275 186 403 801 761 1028 2301 Fragments arranged by size: 2301 1028 801 761 403 275 186 BsiHKAI G_wGCw'C Cuts at: 3816 3901 4972 3816 Size: 85 1071 4599 Fragments arranged by size: 4599 1071 85 BsmI GAATG_Cn' Cuts at: 2192 3266 3359 2192 Size: 1074 93 4588 Fragments arranged by size: 4588 1074 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2043 3074 3816 3901 4972 1391 Size: 399 253 1031 742 85 1071 2174 Fragments arranged by size: 2174 1071 1031 742 399 253 85 BspEI T'CCGG_A Cuts at: 2685 2961 5418 2685 Size: 276 2457 3022 Fragments arranged by size: 3022 2457 276 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 844 Size: 2266 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2173 4250 4424 65 Size: 1183 925 2077 174 1396 Fragments arranged by size: 2077 1396 1183 925 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2453 2912 4399 4586 1700 Size: 110 643 459 1487 187 2869 Fragments arranged by size: 2869 1487 643 459 187 110 BsrGI T'GTAC_A Cuts at: 96 2293 96 Size: 2197 3558 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5755 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstYI r'GATC_y Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750 Size: 1113 367 385 17 1452 86 12 346 Cuts at: 5750 1972 Size: 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2478 2607 2985 513 Size: 552 1413 129 378 3283 Fragments arranged by size: 3283 1413 552 378 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 2748 3057 3271 4065 4085 2748 Size: 309 214 794 20 4418 Fragments arranged by size: 4418 794 309 214 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2980 4762 809 Size: 507 1664 1782 1802 Fragments arranged by size: 1802 1782 1664 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2385 2610 2748 Size: 54 1425 50 275 18 555 225 138 Cuts at: 2748 2910 2914 3202 4091 5720 8 Size: 162 4 288 889 1629 43 Fragments arranged by size: 1629 1425 889 555 288 275 225 162 138 54 50 43 18 4 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2973 3685 1353 Size: 1620 712 3423 Fragments arranged by size: 3423 1620 712 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2828 2997 3818 4415 5202 5470 1239 Size: 393 1196 169 821 597 787 268 1524 Fragments arranged by size: 1524 1196 821 787 597 393 268 169 EcoRI G'AATT_C Cuts at: 3064 3442 3064 Size: 378 5377 FseI GG_CCGG'CC Cuts at: 2916 2916 Size: 5755 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 2270 2400 4902 1678 Size: 592 130 2502 2531 Fragments arranged by size: 2531 2502 592 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2240 2272 2801 2833 2987 Size: 32 135 32 668 32 529 32 154 Cuts at: 2987 3019 4396 4428 4470 4502 1373 Size: 32 1377 32 42 32 2626 Fragments arranged by size: 2626 1377 668 529 154 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3099 3345 669 Size: 988 281 1035 126 246 3079 Fragments arranged by size: 3079 1035 988 281 246 126 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 3080 3080 Size: 5755 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4869 5053 1305 Size: 3564 184 2007 Fragments arranged by size: 3564 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2387 2612 2750 10 Size: 54 1425 343 555 225 138 3015 Fragments arranged by size: 3015 1425 555 343 225 138 54 MslI CAynn'nnrTG Cuts at: 518 1166 2046 2078 2903 3713 4072 4231 518 Size: 648 880 32 825 810 359 159 2042 Fragments arranged by size: 2042 880 825 810 648 359 159 32 MspA1I CmG'CkG Cuts at: 1606 3171 3848 4996 5241 1606 Size: 1565 677 1148 245 2120 Fragments arranged by size: 2120 1565 1148 677 245 NaeI GCC'GGC Cuts at: 2455 2914 2455 Size: 459 5296 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5203 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NgoMIV G'CCGG_C Cuts at: 2453 2912 2453 Size: 459 5296 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5755 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2365 3113 4658 1132 Size: 97 1136 748 1545 2229 Fragments arranged by size: 2229 1545 1136 748 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 2361 4654 2361 Size: 2293 3462 PflMI CCAn_nnn'nTGG Cuts at: 1108 3052 1108 Size: 1944 3811 PfoI T'CCnGG_A Cuts at: 2535 2805 2535 Size: 270 5485 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 3325 3325 Size: 5755 PstI C_TGCA'G Cuts at: 830 2333 3107 830 Size: 1503 774 3478 Fragments arranged by size: 3478 1503 774 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 2546 4003 5715 2546 Size: 1457 1712 2586 Fragments arranged by size: 2586 1712 1457 SexAI A'CCwGG_T Cuts at: 2778 2778 Size: 5755 SfcI C'TryA_G Cuts at: 826 1045 2329 2578 3103 4238 4919 5110 826 Size: 219 1284 249 525 1135 681 191 1471 Fragments arranged by size: 1471 1284 1135 681 525 249 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2459 2459 Size: 5755 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2726 3859 4760 5022 820 Size: 197 926 783 1133 901 262 1553 Fragments arranged by size: 1553 1133 926 901 783 262 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2124 3194 513 Size: 552 1059 1070 3074 Fragments arranged by size: 3074 1070 1059 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 2836 3782 3799 3952 4137 4582 Size: 1255 593 663 946 17 153 185 445 Cuts at: 4582 325 Size: 1498 Fragments arranged by size: 1498 1255 946 663 593 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2293 2544 4001 Size: 274 80 33 51 1443 316 251 1457 Cuts at: 4001 5713 96 Size: 1712 138 Fragments arranged by size: 1712 1457 1443 316 274 251 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2458 2490 2811 2840 2843 2872 5715 5747 2458 Size: 32 321 29 3 29 2843 32 2466 Fragments arranged by size: 2843 2466 321 32 32 29 29 3 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2312 2620 2902 2312 Size: 308 282 5165 Fragments arranged by size: 5165 308 282 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 2012 3884 2012 Size: 1872 3883 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfoI SrfI SwaI