################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI Ksp632I KspI MaeI MaeII MfeI MluI MseI MslI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 314 GGTACC G^GTACC AccI 123 GTATAC GT^[AC][GT]AC AccII 130 CGCG CG^CG AccIII 290 TCCGGA T^CCGGA AcyI 586 GACGCC G[GA]^CG[CT]C AfaI 315 GTAC GT^AC Aor51HI 227 AGCGCT AGC^GCT Asp718I 314 GGTACC G^GTACC AvaII 565 GGACC G^G[AT]CC BglI 270 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaBI 294 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 586 GACGCC G[GA]^CG[CT]C BseAI 290 TCCGGA T^CCGGA BsmAI 67 GTCTC GTCTC(1/5) BsmI 367 GAATGC GAATGC(1/-1) BspEI 290 TCCGGA T^CCGGA BspHI 305 TCATGA T^CATGA Bst1107I 123 GTATAC GTA^TAC BstUI 130 CGCG CG^CG BstXI 194 CCAAACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 299 AGATCC [GA]^GATC[CT] BstZ17I 123 GTATAC GTA^TAC DsaI 188 CCGTGG C^C[GA][CT]GG Eco47III 227 AGCGCT AGC^GCT EcoT14I 608 CCAAGG C^C[AT][AT]GG HgaI 586 GACGC GACGC(5/10) Hin1I 586 GACGCC G[GA]^CG[CT]C HinfI 460 GACTC G^A[ACGT]TC HphI 494 TCACC GGTGA(8/7) KpnI 314 GGTACC GGTAC^C MaeIII 217 GTGAC ^GT[ACGT]AC MamI 294 GATGCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 349 GAAGA GAAGA(8/7) MflI 299 AGATCC [GA]^GATC[CT] MroI 290 TCCGGA T^CCGGA MvnI 130 CGCG CG^CG PleI 460 GACTC GAGTC(4/5) PstI 40 CTGCAG CTGCA^G RcaI 305 TCATGA T^CATGA RsaI 315 GTAC GT^AC SacI 175 GAGCTC GAGCT^C SfcI 40 CTGCAG C^T[GA][CT]AG SmaI 146 CCCGGG CCC^GGG StyI 608 CCAAGG C^C[AT][AT]GG Tsp45I 217 GTGAC ^GT[GC]AC XhoII 299 AGATCC [GA]^GATC[CT] XmaCI 146 CCCGGG C^CCGGG XmaI 146 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 300 GATCC GGATC(4/5) 309 GATCC AspHI 175 GAGCTC G[AT]GC[AT]^C 473 GAGCAC AvaI 146 CCCGGG C^[CT]CG[GA]G 318 CCCGAG BalI 87 TGGCCA TGG^CCA 191 TGGCCA BanII 3 GAGCCC G[GA]GC[CT]^C 175 GAGCTC BpmI 506 CTCCAG CTGGAG(16/14) 259 CTGGAG BsaWI 290 TCCGGA [AT]^CCGG[AT] 311 TCCGGT BsgI 533 CTGCAC GTGCAG(16/14) 223 GTGCAG BsiHKAI 175 GAGCTC G[AT]GC[AT]^C 473 GAGCAC BsoBI 146 CCCGGG C^[CT]CG[GA]G 318 CCCGAG BspMI 136 ACCTGC ACCTGC(4/8) 567 ACCTGC BsrI 274 ACTGG ACTGG(1/-1) 360 CCAGT BstF5I 499 CATCC GGATG(2/0) 293 GGATG DpnII 300 GATC ^GATC 309 GATC DraII 59 AGGCCCC [GA]G^G[ACGT]CC[CT] 516 AGGCCCC EaeI 87 TGGCCA [CT]^GGCC[GA] 191 TGGCCA EcoO109I 59 AGGCCCC [GA]G^G[ACGT]CC[CT] 516 AGGCCCC FauI 52 CCCGC CCCGC(4/6) 614 GCGGG FokI 499 CATCC GGATG(9/13) 293 GGATG HaeII 227 AGCGCT [GA]GCGC^[CT] 550 AGCGCC MboI 300 GATC ^GATC 309 GATC MluNI 87 TGGCCA TGG^CCA 191 TGGCCA MscI 87 TGGCCA TGG^CCA 191 TGGCCA NdeII 300 GATC ^GATC 309 GATC Sau3AI 300 GATC ^GATC 309 GATC SfaNI 79 GATGC GCATC(5/9) 294 GATGC ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstNI Cac8I CfoI Cfr13I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PvuII Sau96I ScrFI TseI TspRI