################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BsaBI BsaI BsiEI BsiWI BsmAI BsmBI BspDI BspLU11I BspMI BsrBI BssHII Bst1107I BstBI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MluI MluNI MscI MseI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SnaBI SpeI SphI Sse8387I SspI SwaI TfiI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 55 GTCTAC GT^[AC][GT]AC AccIII 62 TCCGGA T^CCGGA AcyI 169 GACGCC G[GA]^CG[CT]C Alw44I 624 GTGCAC G^TGCAC AlwNI 903 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 624 GTGCAC G^TGCAC AvaI 506 CCCGGG C^[CT]CG[GA]G BanI 862 GGCACC G^G[CT][GA]CC BanII 286 GAGCTC G[GA]GC[CT]^C BbsI 274 GTCTTC GAAGAC(2/6) BglI 337 GCCGGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 574 CTGGAG CTGGAG(16/14) BpuAI 274 GTCTTC GAAGAC(2/6) BsaHI 169 GACGCC G[GA]^CG[CT]C BseAI 62 TCCGGA T^CCGGA BsmFI 428 GGGAC GGGAC(10/14) BsmI 522 GAATGC GAATGC(1/-1) BsoBI 506 CCCGGG C^[CT]CG[GA]G BspEI 62 TCCGGA T^CCGGA BspHI 536 TCATGA T^CATGA BssSI 621 CTCGTG CACGAG(-5/-1) BstAPI 1007 GCAACACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 846 GGTGACC G^GT[ACGT]ACC BstPI 846 GGTGACC G^GT[ACGT]ACC DraII 839 AGGACCC [GA]G^G[ACGT]CC[CT] DraIII 607 CACGGCGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 237 TGGCCG [CT]^GGCC[GA] EcoO109I 839 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO65I 846 GGTGACC G^GT[ACGT]ACC HaeII 17 GGCGCT [GA]GCGC^[CT] HgaI 169 GACGC GACGC(5/10) Hin1I 169 GACGCC G[GA]^CG[CT]C HinfI 531 GACTC G^A[ACGT]TC MroI 62 TCCGGA T^CCGGA MslI 1026 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 788 CCATGG C^CATGG PleI 531 GACTC GAGTC(4/5) PpuMI 839 AGGACCC [GA]G^G[AT]CC[CT] PshAI 211 GACGAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 359 CAGCTG CAG^CTG RcaI 536 TCATGA T^CATGA SacI 286 GAGCTC GAGCT^C SexAI 800 ACCTGGT A^CC[AT]GGT SgrAI 336 CGCCGGCG C[GA]^CCGG[CT]G SmaI 506 CCCGGG CCC^GGG SmlI 682 CTCAAG C^T[CT][GA]AG StuI 126 AGGCCT AGG^CCT TaqI 812 TCGA T^CGA TthHB8I 812 TCGA T^CGA XcmI 488 CCAAGGTCTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 506 CCCGGG C^CCGGG XmaI 506 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AflIII 1010 ACACGT A^C[GA][CT]GT 1037 ACACGT BcgI 269 GCACCGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 476 GCATCGGCTTCG BsaWI 5 ACCGGA [AT]^CCGG[AT] 62 TCCGGA BsgI 254 CTGCAC GTGCAG(16/14) 1014 GTGCAG Bsp1407I 806 TGTACA T^GTACA 1034 TGTACA BsrDI 330 CATTGC GCAATG(2/0) 233 GCAATG BsrGI 806 TGTACA T^GTACA 1034 TGTACA BstF5I 465 CATCC GGATG(2/0) 501 GGATG BstYI 119 AGATCC [GA]^GATC[CT] 569 AGATCC DsaI 606 CCACGG C^C[GA][CT]GG 788 CCATGG EcoT14I 488 CCAAGG C^C[AT][AT]GG 788 CCATGG FauI 956 CCCGC CCCGC(4/6) 144 GCGGG FokI 465 CATCC GGATG(9/13) 501 GGATG HphI 846 GGTGA GGTGA(8/7) 317 TCACC MboII 756 GAAGA GAAGA(8/7) 275 TCTTC MflI 119 AGATCC [GA]^GATC[CT] 569 AGATCC NaeI 161 GCCGGC GCC^GGC 337 GCCGGC NgoMIV 161 GCCGGC G^CCGGC 337 GCCGGC SspBI 806 TGTACA T^GTACA 1034 TGTACA StyI 488 CCAAGG C^C[AT][AT]GG 788 CCATGG Tsp45I 847 GTGAC ^GT[GC]AC 911 GTGAC XhoII 119 AGATCC [GA]^GATC[CT] 569 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AspHI AvaII BbrPI BbvI BcnI BmyI BsaAI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrFI BsrI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MaeII MaeIII MboI MnlI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PmaCI PmlI RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI