Restriction map for
pFN21AE1440
(Circular) (Six-base) MAPSORT of: pFN21AE1440.seq Check: 232 from: 1 to: 5752
pFN21AE1440 5752 bp
With 182 enzymes: SgfI *
August 16, 2010 14:52 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2061 3095 1937
Size: 124 1034 4594
Fragments arranged by size:
4594 1034 124
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2780 3815 5199 5467 1632
Size: 1148 1035 1384 268 1917
Fragments arranged by size:
1917 1384 1148 1035 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 3015 3042 4651 5628 1203
Size: 1812 27 1609 977 1327
Fragments arranged by size:
1812 1609 1327 977 27
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5752
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5752
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2303 2335 2303
Size: 32 5720
AlwNI CAG_nnn'CTG
Cuts at: 2913 3495 5067 2913
Size: 582 1572 3598
Fragments arranged by size:
3598 1572 582
ApaLI G'TGCA_C
Cuts at: 2629 3809 4965 2629
Size: 1180 1156 3416
Fragments arranged by size:
3416 1180 1156
ApoI r'AATT_y
Cuts at: 1493 3061 3287 3439 5548 1493
Size: 1568 226 152 2109 1697
Fragments arranged by size:
2109 1697 1568 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2511 3077 1870
Size: 73 568 566 4545
Fragments arranged by size:
4545 568 566 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2765 2798 2765
Size: 33 5719
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2867 3073 4528 618
Size: 325 217 627 1080 206 1455 1842
Fragments arranged by size:
1842 1455 1080 627 325 217 206
BanII G_rGCy'C
Cuts at: 1391 2295 3071 1391
Size: 904 776 4072
Fragments arranged by size:
4072 904 776
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2272 928
Size: 971 373 4408
Fragments arranged by size:
4408 971 373
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2263 2297 2470 2504 3943 3977 1903
Size: 34 326 34 173 34 1439 34 3678
Fragments arranged by size:
3678 1439 326 173 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2348 4363 136
Size: 107 122 71 631 605 676 2015 1525
Fragments arranged by size:
2015 1525 676 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2633 2870 3813 4969 1790
Size: 843 237 943 1156 2573
Fragments arranged by size:
2573 1156 943 843 237
BmgBI CAC'GTC
Cuts at: 1318 2643 1318
Size: 1325 4427
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 3483 4441
Size: 416 53 83 399 24 856 1210 958
Cuts at: 4441 442
Size: 1753
Fragments arranged by size:
1753 1210 958 856 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2600 4412 1239
Size: 1361 1812 2579
Fragments arranged by size:
2579 1812 1361
Bpu10I CC'TnA_GC
Cuts at: 2387 2387
Size: 5752
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2672 3877 4742 5040 2672
Size: 1205 865 298 3384
Fragments arranged by size:
3384 1205 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2683 2716 3018 3045 3543 493
Size: 711 1479 33 302 27 498 2702
Fragments arranged by size:
2702 1479 711 498 302 33 27
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2175 3559 3941 4576
Size: 53 83 186 936 642 1384 382 635
Cuts at: 4576 275
Size: 1451
Fragments arranged by size:
1451 1384 936 642 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2010 2067 4185 4583 4857 5004 5415 2010
Size: 57 2118 398 274 147 411 2347
Fragments arranged by size:
2347 2118 411 398 274 147 57
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2303 2333 1178
Size: 30 165 30 900 30 4597
Fragments arranged by size:
4597 900 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2074 2597 2855 1186
Size: 888 523 258 4083
Fragments arranged by size:
4083 888 523 258
BseYI C'CCAG_C
Cuts at: 2674 4955 5451 2674
Size: 2281 496 2975
Fragments arranged by size:
2975 2281 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2244 3040 1163
Size: 573 508 796 3875
Fragments arranged by size:
3875 796 573 508
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3202 3963 4991 1540
Size: 275 186 1201 761 1028 2301
Fragments arranged by size:
2301 1201 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2295 2633 2688 3813 3898 4969 2295
Size: 338 55 1125 85 1071 3078
Fragments arranged by size:
3078 1125 1071 338 85 55
BsmI GAATG_Cn'
Cuts at: 2533 3263 3356 2533
Size: 730 93 4929
Fragments arranged by size:
4929 730 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2295 2633 2688 2870 3071 3813 3898
Size: 399 505 338 55 182 201 742 85
Cuts at: 3898 4969 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 505 399 338 201 182
85 55
BspEI T'CCGG_A
Cuts at: 2067 5415 2067
Size: 3348 2404
BspHI T'CATG_A
Cuts at: 2541 3641 5371 2541
Size: 1100 1730 2922
Fragments arranged by size:
2922 1730 1100
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3107 844
Size: 2263 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3639 1394
Size: 429 1816 3507
Fragments arranged by size:
3507 1816 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2245 2334 4247 4421 65
Size: 1183 997 89 1913 174 1396
Fragments arranged by size:
1913 1396 1183 997 174 89
BsrFI r'CCGG_y
Cuts at: 1700 1810 2166 2342 2855 4396 4583 1700
Size: 110 356 176 513 1541 187 2869
Fragments arranged by size:
2869 1541 513 356 187 176 110
BsrGI T'GTAC_A
Cuts at: 96 2811 3039 96
Size: 2715 228 2809
Fragments arranged by size:
2809 2715 228
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5752
BssSI C'ACGA_G
Cuts at: 2626 3812 4824 2626
Size: 1186 1012 3554
Fragments arranged by size:
3554 1186 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3018 794
Size: 2224 3528
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 2851 1184
Size: 1667 4085
BstYI r'GATC_y
Cuts at: 1972 2124 2574 3082 3449 3834 3851 5303 5389
Size: 152 450 508 367 385 17 1452 86
Cuts at: 5389 5401 5747 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 508 450 385 367 346 152
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2611 2793 513
Size: 552 1546 182 3472
Fragments arranged by size:
3472 1546 552 182
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5013
Fragments arranged by size:
5013 683 56
BtsI GCAGTG_nn'
Cuts at: 2654 2866 2978 3268 4062 4082 2654
Size: 212 112 290 794 20 4324
Fragments arranged by size:
4324 794 290 212 112 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DraIII CAC_nnn'GTG
Cuts at: 2617 2617
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 3199 4088
Size: 54 1425 50 275 18 412 957 889
Cuts at: 4088 5717 8
Size: 1629 43
Fragments arranged by size:
1629 1425 957 889 412 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3682 1353
Size: 2329 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2293 2293
Size: 5752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2600 2780 3815 4412 5199 5467 1239
Size: 393 968 180 1035 597 787 268 1524
Fragments arranged by size:
1524 1035 968 787 597 393 268 180
EcoO109I rG'GnC_Cy
Cuts at: 2845 2845
Size: 5752
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2026 4899 1678
Size: 348 2873 2531
Fragments arranged by size:
2873 2531 348
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3351 3351
Size: 5752
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3930
Fragments arranged by size:
3930 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3035 3710 4069 4228 518
Size: 648 1869 675 359 159 2042
Fragments arranged by size:
2042 1869 675 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2149 2247 2366 2910 3168 3845 4993 5238
Size: 543 98 119 544 258 677 1148 245
Cuts at: 5238 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 544 543 258 245 119
98
NaeI GCC'GGC
Cuts at: 2168 2344 2168
Size: 176 5576
NcoI C'CATG_G
Cuts at: 513 1065 2793 513
Size: 552 1728 3472
Fragments arranged by size:
3472 1728 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NgoMIV G'CCGG_C
Cuts at: 2166 2342 2166
Size: 176 5576
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 3110 4655 1132
Size: 97 1881 1545 2229
Fragments arranged by size:
2229 1881 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2377 2872 1348
Size: 1029 495 4228
Fragments arranged by size:
4228 1029 495
PciI A'CATG_T
Cuts at: 4651 4651
Size: 5752
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5752
PfoI T'CCnGG_A
Cuts at: 2577 2577
Size: 5752
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PmlI CAC'GTG
Cuts at: 2716 3018 3045 2716
Size: 302 27 5423
Fragments arranged by size:
5423 302 27
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2303 2335 3821 3853 5334 5366 2303
Size: 32 1486 32 1481 32 2689
Fragments arranged by size:
2689 1486 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2845 2845
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 2220 1613
Size: 607 5145
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
PvuII CAG'CTG
Cuts at: 2366 2366
Size: 5752
SacI G_AGCT'C
Cuts at: 2295 2295
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SexAI A'CCwGG_T
Cuts at: 2805 2805
Size: 5752
SfcI C'TryA_G
Cuts at: 826 1045 3100 4235 4916 5107 826
Size: 219 2055 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 2342 1700
Size: 642 5110
SmaI CCC'GGG
Cuts at: 1872 2513 3079 1872
Size: 641 566 4545
Fragments arranged by size:
4545 641 566
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2687 3856 4757 5019 820
Size: 197 926 744 1169 901 262 1553
Fragments arranged by size:
1553 1169 926 901 744 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2133 1517
Size: 616 5136
StyI C'CwwG_G
Cuts at: 513 1065 2493 2793 3191 513
Size: 552 1428 300 398 3074
Fragments arranged by size:
3074 1428 552 398 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2885 2963 3779 3796 3949 4134 4579
Size: 1255 1305 78 816 17 153 185 445
Cuts at: 4579 325
Size: 1498
Fragments arranged by size:
1498 1305 1255 816 445 185 153 78
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2697 2811 3039
Size: 274 80 33 51 1443 720 114 228
Cuts at: 3039 3998 5710 96
Size: 959 1712 138
Fragments arranged by size:
1712 1443 959 720 274 228 138 114
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2437 2469 2591 2623 5712 5744 2437
Size: 32 122 32 3089 32 2445
Fragments arranged by size:
3089 2445 122 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2500 2500
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2511 3077 1870
Size: 641 566 4545
Fragments arranged by size:
4545 641 566
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI
SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV
FalI FseI FspAI KasI NarI NruI NsiI PacI
PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI
SfoI SrfI SwaI