(Linear) (Six-base) MAPSORT of: KIEE1440.seq Check: 1561 from: 1 to: 1047 KIEE1440.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. AccI GT'mk_AC Cuts at: 0 56 1047 Size: 56 991 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 775 1047 Size: 775 272 AflIII A'CryG_T Cuts at: 0 1010 1037 1047 Size: 1010 27 10 Fragments arranged by size: 1010 27 10 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 298 330 1047 Size: 298 32 717 Fragments arranged by size: 717 298 32 AlwNI CAG_nnn'CTG Cuts at: 0 908 1047 Size: 908 139 ApaLI G'TGCA_C Cuts at: 0 624 1047 Size: 624 423 AvaI C'yCGr_G Cuts at: 0 506 1047 Size: 506 541 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 760 793 1047 Size: 760 33 254 Fragments arranged by size: 760 254 33 BanI G'GyrC_C Cuts at: 0 862 1047 Size: 862 185 BanII G_rGCy'C Cuts at: 0 290 1047 Size: 290 757 BbsI GAAGACnn'nnnn_ Cuts at: 0 267 1047 Size: 267 780 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 258 292 465 499 1047 Size: 258 34 173 34 548 Fragments arranged by size: 548 258 173 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 343 1047 Size: 343 704 Bme1580I G_kGCm'C Cuts at: 0 628 865 1047 Size: 628 237 182 Fragments arranged by size: 628 237 182 BmgBI CAC'GTC Cuts at: 0 638 1047 Size: 638 409 BmrI ACTGGGnnnn_n' Cuts at: 0 268 1047 Size: 268 779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 595 1047 Size: 595 452 Bpu10I CC'TnA_GC Cuts at: 0 382 1047 Size: 382 665 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 667 1047 Size: 667 380 BsaAI yAC'GTr Cuts at: 0 678 711 1013 1040 1047 Size: 678 33 302 27 7 Fragments arranged by size: 678 302 33 27 7 BsaHI Gr'CG_yC Cuts at: 0 170 1047 Size: 170 877 BsaWI w'CCGG_w Cuts at: 0 5 62 1047 Size: 5 57 985 Fragments arranged by size: 985 57 5 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 298 328 1047 Size: 298 30 719 Fragments arranged by size: 719 298 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 69 592 850 1047 Size: 69 523 258 197 Fragments arranged by size: 523 258 197 69 BseYI C'CCAG_C Cuts at: 0 669 1047 Size: 669 378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 239 1035 1047 Size: 239 796 12 Fragments arranged by size: 796 239 12 BsiHKAI G_wGCw'C Cuts at: 0 290 628 683 1047 Size: 290 338 55 364 Fragments arranged by size: 364 338 290 55 BsmI GAATG_Cn' Cuts at: 0 528 1047 Size: 528 519 Bsp1286I G_dGCh'C Cuts at: 0 290 628 683 865 1047 Size: 290 338 55 182 182 Fragments arranged by size: 338 290 182 182 55 BspEI T'CCGG_A Cuts at: 0 62 1047 Size: 62 985 BspHI T'CATG_A Cuts at: 0 536 1047 Size: 536 511 BsrDI GCAATG_nn' Cuts at: 0 240 329 1047 Size: 240 89 718 Fragments arranged by size: 718 240 89 BsrFI r'CCGG_y Cuts at: 0 161 337 850 1047 Size: 161 176 513 197 Fragments arranged by size: 513 197 176 161 BsrGI T'GTAC_A Cuts at: 0 806 1034 1047 Size: 806 228 13 Fragments arranged by size: 806 228 13 BssSI C'ACGA_G Cuts at: 0 621 1047 Size: 621 426 BstAPI GCAn_nnn'nTGC Cuts at: 0 1013 1047 Size: 1013 34 BstEII G'GTnAC_C Cuts at: 0 846 1047 Size: 846 201 BstYI r'GATC_y Cuts at: 0 119 569 1047 Size: 119 450 478 Fragments arranged by size: 478 450 119 BtgI C'CryG_G Cuts at: 0 606 788 1047 Size: 606 182 259 Fragments arranged by size: 606 259 182 BtsI GCAGTG_nn' Cuts at: 0 649 861 973 1047 Size: 649 212 112 74 Fragments arranged by size: 649 212 112 74 DraIII CAC_nnn'GTG Cuts at: 0 612 1047 Size: 612 435 EaeI y'GGCC_r Cuts at: 0 237 1047 Size: 237 810 EcoICRI GAG'CTC Cuts at: 0 288 1047 Size: 288 759 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 595 775 1047 Size: 595 180 272 Fragments arranged by size: 595 272 180 EcoO109I rG'GnC_Cy Cuts at: 0 840 1047 Size: 840 207 HaeII r_GCGC'y Cuts at: 0 21 1047 Size: 21 1026 MslI CAynn'nnrTG Cuts at: 0 1030 1047 Size: 1030 17 MspA1I CmG'CkG Cuts at: 0 144 242 361 905 1047 Size: 144 98 119 544 142 Fragments arranged by size: 544 144 142 119 98 NaeI GCC'GGC Cuts at: 0 163 339 1047 Size: 163 176 708 Fragments arranged by size: 708 176 163 NcoI C'CATG_G Cuts at: 0 788 1047 Size: 788 259 NgoMIV G'CCGG_C Cuts at: 0 161 337 1047 Size: 161 176 710 Fragments arranged by size: 710 176 161 PasI CC'CwG_GG Cuts at: 0 372 867 1047 Size: 372 495 180 Fragments arranged by size: 495 372 180 PfoI T'CCnGG_A Cuts at: 0 572 1047 Size: 572 475 PmlI CAC'GTG Cuts at: 0 711 1013 1040 1047 Size: 711 302 27 7 Fragments arranged by size: 711 302 27 7 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 298 330 1047 Size: 298 32 717 Fragments arranged by size: 717 298 32 PpuMI rG'GwC_Cy Cuts at: 0 840 1047 Size: 840 207 PshAI GACnn'nnGTC Cuts at: 0 215 1047 Size: 215 832 PvuII CAG'CTG Cuts at: 0 361 1047 Size: 361 686 SacI G_AGCT'C Cuts at: 0 290 1047 Size: 290 757 SexAI A'CCwGG_T Cuts at: 0 800 1047 Size: 800 247 SgrAI Cr'CCGG_yG Cuts at: 0 337 1047 Size: 337 710 SmaI CCC'GGG Cuts at: 0 508 1047 Size: 508 539 SmlI C'TyrA_G Cuts at: 0 682 1047 Size: 682 365 StuI AGG'CCT Cuts at: 0 128 1047 Size: 128 919 StyI C'CwwG_G Cuts at: 0 488 788 1047 Size: 488 300 259 Fragments arranged by size: 488 300 259 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 880 958 1047 Size: 880 78 89 Fragments arranged by size: 880 89 78 TatI w'GTAC_w Cuts at: 0 692 806 1034 1047 Size: 692 114 228 13 Fragments arranged by size: 692 228 114 13 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 432 464 586 618 1047 Size: 432 32 122 32 429 Fragments arranged by size: 432 429 122 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 495 1047 Size: 495 552 XmaI C'CCGG_G Cuts at: 0 506 1047 Size: 506 541 Enzymes that do cut: AccI AcuI AflIII AloI AlwNI ApaLI AvaI BaeI BanI BanII BbsI BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstYI BtgI BtsI DraIII EaeI EcoICRI Eco57MI EcoO109I HaeII MslI MspA1I NaeI NcoI NgoMIV PasI PfoI PmlI PpiI PpuMI PshAI PvuII SacI SexAI SgrAI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI ApaI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BciVI BclI BfrBI BglII BlpI BmtI BplI BsaI BsaBI BsiEI BsiWI BsmBI BspMI BsrBI BssHII BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PflMI PmeI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI