(Circular) (Six-base) MAPSORT of: pFN21AB4614.seq Check: 5196 from: 1 to: 6493 pFN21AB4614 6493 bp With 182 enzymes: SgfI * August 16, 2010 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3814 3814 Size: 6493 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2829 2919 4556 5940 6208 1632 Size: 1197 90 1637 1384 268 1917 Fragments arranged by size: 1917 1637 1384 1197 268 90 AfeI AGC'GCT Cuts at: 2590 2741 3142 2590 Size: 151 401 5941 Fragments arranged by size: 5941 401 151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 3651 5392 6369 1203 Size: 2448 1741 977 1327 Fragments arranged by size: 2448 1741 1327 977 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 2636 3215 1166 Size: 1470 579 4444 Fragments arranged by size: 4444 1470 579 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2565 1184 Size: 1381 5112 AlwNI CAG_nnn'CTG Cuts at: 4236 5808 4236 Size: 1572 4921 ApaLI G'TGCA_C Cuts at: 4550 5706 4550 Size: 1156 5337 ApoI r'AATT_y Cuts at: 1493 3332 3422 3629 3787 3802 4028 4180 6289 Size: 1839 90 207 158 15 226 152 2109 Cuts at: 6289 1493 Size: 1697 Fragments arranged by size: 2109 1839 1697 226 207 158 152 90 15 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 2984 3818 1870 Size: 73 1041 834 4545 Fragments arranged by size: 4545 1041 834 73 BamHI G'GATC_C Cuts at: 2684 3062 3823 4190 2684 Size: 378 761 367 4987 Fragments arranged by size: 4987 761 378 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2386 3163 3321 3814 5269 Size: 325 217 627 599 777 158 493 1455 Cuts at: 5269 618 Size: 1842 Fragments arranged by size: 1842 1455 777 627 599 493 325 217 158 BanII G_rGCy'C Cuts at: 1391 3397 3812 1391 Size: 2006 415 4072 Fragments arranged by size: 4072 2006 415 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2403 2817 928 Size: 971 504 414 4604 Fragments arranged by size: 4604 971 504 414 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4684 4718 1903 Size: 34 2747 34 3678 Fragments arranged by size: 3678 2747 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2797 4385 5595 2797 Size: 1588 1210 3695 Fragments arranged by size: 3695 1588 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6488 6488 Size: 6493 BlpI GC'TnA_GC Cuts at: 3228 3910 3228 Size: 682 5811 Bme1580I G_kGCm'C Cuts at: 1790 2328 4554 5710 1790 Size: 538 2226 1156 2573 Fragments arranged by size: 2573 2226 1156 538 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2883 3317 4224 Size: 416 53 83 399 24 1466 434 907 Cuts at: 4224 5182 442 Size: 958 1753 Fragments arranged by size: 1753 1466 958 907 434 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2666 1056 Size: 1610 4883 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5153 1239 Size: 3914 2579 Bpu10I CC'TnA_GC Cuts at: 2274 2274 Size: 6493 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4618 5483 5781 4618 Size: 865 298 5330 Fragments arranged by size: 5330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5156 882 Size: 617 3657 2219 Fragments arranged by size: 3657 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2818 3072 4300 4682 Size: 53 83 186 936 1285 254 1228 382 Cuts at: 4682 5317 275 Size: 635 1451 Fragments arranged by size: 1451 1285 1228 936 635 382 254 186 83 53 BsaWI w'CCGG_w Cuts at: 4926 5324 5598 5745 6156 4926 Size: 398 274 147 411 5263 Fragments arranged by size: 5263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6268 Fragments arranged by size: 6268 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2505 3398 1186 Size: 1319 893 4281 Fragments arranged by size: 4281 1319 893 BseYI C'CCAG_C Cuts at: 2035 2458 3192 5696 6192 2035 Size: 423 734 2504 496 2336 Fragments arranged by size: 2504 2336 734 496 423 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 2565 1163 Size: 573 511 318 5091 Fragments arranged by size: 5091 573 511 318 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3943 4704 5732 1540 Size: 275 186 1942 761 1028 2301 Fragments arranged by size: 2301 1942 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3234 4554 4639 5710 3234 Size: 1320 85 1071 4017 Fragments arranged by size: 4017 1320 1071 85 BsmI GAATG_Cn' Cuts at: 4004 4097 4004 Size: 93 6400 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2328 3234 3397 3812 4554 4639 5710 Size: 399 538 906 163 415 742 85 1071 Cuts at: 5710 1391 Size: 2174 Fragments arranged by size: 2174 1071 906 742 538 415 399 163 85 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 4382 6112 4382 Size: 1730 4763 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3848 844 Size: 3004 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4380 1394 Size: 429 2557 3507 Fragments arranged by size: 3507 2557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4988 5162 65 Size: 1183 3740 174 1396 Fragments arranged by size: 3740 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2278 5137 5324 1700 Size: 110 468 2859 187 2869 Fragments arranged by size: 2869 2859 468 187 110 BsrGI T'GTAC_A Cuts at: 96 2222 96 Size: 2126 4367 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 2261 794 Size: 1467 5026 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 3022 1184 Size: 1838 4655 BstXI CCAn_nnnn'nTGG Cuts at: 2043 2873 2043 Size: 830 5663 BstYI r'GATC_y Cuts at: 1972 2684 3062 3459 3823 4190 4575 4592 6044 Size: 712 378 397 364 367 385 17 1452 Cuts at: 6044 6130 6142 6488 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 712 397 385 378 367 364 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2390 2499 2615 3619 513 Size: 552 1325 109 116 1004 3387 Fragments arranged by size: 3387 1325 1004 552 116 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2160 487 Size: 683 56 934 4820 Fragments arranged by size: 4820 934 683 56 BtsI GCAGTG_nn' Cuts at: 3346 4009 4803 4823 3346 Size: 663 794 20 5016 Fragments arranged by size: 5016 794 663 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3797 3797 Size: 6493 DraIII CAC_nnn'GTG Cuts at: 2734 2734 Size: 6493 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3098 3940 4829 Size: 54 1425 50 275 18 1268 842 889 Cuts at: 4829 6458 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1268 889 842 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3940 1537 Size: 275 2128 4090 Fragments arranged by size: 4090 2128 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2674 2783 4423 1353 Size: 1321 109 1640 3423 Fragments arranged by size: 3423 1640 1321 109 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 5454 5600 387 Size: 1064 138 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2829 2919 4556 5153 5940 6208 1239 Size: 393 1197 90 1637 597 787 268 1524 Fragments arranged by size: 1637 1524 1197 787 597 393 268 90 EcoRI G'AATT_C Cuts at: 3332 3802 4180 3332 Size: 470 378 5645 Fragments arranged by size: 5645 470 378 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 2592 2743 3144 5640 1678 Size: 914 151 401 2496 2531 Fragments arranged by size: 2531 2496 914 401 151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3459 3491 5134 5166 5208 Size: 32 135 32 1887 32 1643 32 42 Cuts at: 5208 5240 1373 Size: 32 2626 Fragments arranged by size: 2626 1887 1643 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3837 4083 669 Size: 988 281 1899 246 3079 Fragments arranged by size: 3079 1899 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6493 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3818 3818 Size: 6493 MfeI C'AATT_G Cuts at: 4092 4092 Size: 6493 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5607 5791 1305 Size: 4302 184 2007 Fragments arranged by size: 4302 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4671 Fragments arranged by size: 4671 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2547 2636 2775 3215 3624 4451 4810 Size: 648 1381 89 139 440 409 827 359 Cuts at: 4810 4969 518 Size: 159 2042 Fragments arranged by size: 2042 1381 827 648 440 409 359 159 139 89 MspA1I CmG'CkG Cuts at: 1606 3044 3909 4586 5734 5979 1606 Size: 1438 865 677 1148 245 2120 Fragments arranged by size: 2120 1438 1148 865 677 245 NcoI C'CATG_G Cuts at: 513 1065 3619 513 Size: 552 2554 3387 Fragments arranged by size: 3387 2554 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6493 NheI G'CTAG_C Cuts at: 1052 2662 1052 Size: 1610 4883 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 2083 2486 2546 2983 3503 3655 3776 Size: 97 854 403 60 437 520 152 121 Cuts at: 3776 3851 5396 1132 Size: 75 1545 2229 Fragments arranged by size: 2229 1545 854 520 437 403 152 121 97 75 60 PasI CC'CwG_GG Cuts at: 1348 2689 1348 Size: 1341 5152 PciI A'CATG_T Cuts at: 3651 5392 3651 Size: 1741 4752 PflMI CCAn_nnn'nTGG Cuts at: 1108 2828 3008 1108 Size: 1720 180 4593 Fragments arranged by size: 4593 1720 180 PfoI T'CCnGG_A Cuts at: 3657 3657 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2817 1613 Size: 1204 5289 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PstI C_TGCA'G Cuts at: 830 2061 2605 3043 3845 830 Size: 1231 544 438 802 3478 Fragments arranged by size: 3478 1231 802 544 438 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 PvuII CAG'CTG Cuts at: 3044 3044 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 4741 6453 4741 Size: 1712 4781 SexAI A'CCwGG_T Cuts at: 3276 3276 Size: 6493 SfcI C'TryA_G Cuts at: 826 1045 2014 2057 2601 3039 3057 3841 4976 Size: 219 969 43 544 438 18 784 1135 Cuts at: 4976 5657 5848 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 969 784 681 544 438 219 191 43 18 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4597 5498 5760 820 Size: 197 926 2654 901 262 1553 Fragments arranged by size: 2654 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 2546 2983 3851 1132 Size: 1414 437 868 3774 Fragments arranged by size: 3774 1414 868 437 SspI AAT'ATT Cuts at: 5 52 4417 5 Size: 47 4365 2081 Fragments arranged by size: 4365 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6493 StyI C'CwwG_G Cuts at: 513 1065 2042 2822 3106 3463 3619 3932 513 Size: 552 977 780 284 357 156 313 3074 Fragments arranged by size: 3074 977 780 552 357 313 284 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2281 3339 4520 4537 4690 4875 5320 Size: 1255 701 1058 1181 17 153 185 445 Cuts at: 5320 325 Size: 1498 Fragments arranged by size: 1498 1255 1181 1058 701 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2222 4739 6451 Size: 274 80 33 51 1443 245 2517 1712 Cuts at: 6451 96 Size: 138 Fragments arranged by size: 2517 1712 1443 274 245 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6453 6485 6453 Size: 32 6461 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2368 3178 2368 Size: 810 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI