################################## # NO CUT ENZYMES ( 115 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BbvCI BcgI BciVI BclI BfrI BglII BsaBI BsaHI BsaI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BspHI BspMI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI ScaI SexAI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 69 enzymes) # ################################### AccII 1023 CGCG CG^CG AccIII 506 TCCGGA T^CCGGA AflIII 841 ACATGT A^C[GA][CT]GT AspHI 719 GAGCAC G[AT]GC[AT]^C AvaI 827 CCCGGG C^[CT]CG[GA]G AvaII 704 GGTCC G^G[AT]CC AvrII 573 CCTAGG C^CTAGG BalI 407 TGGCCA TGG^CCA BamHI 1200 GGATCC G^GATCC BanI 774 GGTGCC G^G[CT][GA]CC BanII 47 GGGCTC G[GA]GC[CT]^C BbrPI 1228 CACGTG CAC^GTG BlnI 573 CCTAGG C^CTAGG BlpI 682 GCTAAGC GC^T[ACGT]AGC Bpu1102I 682 GCTAAGC GC^T[ACGT]AGC BsaAI 1228 CACGTG [CT]AC^GT[GA] BsaWI 506 TCCGGA [AT]^CCGG[AT] BseAI 506 TCCGGA T^CCGGA BsiHKAI 719 GAGCAC G[AT]GC[AT]^C BsoBI 827 CCCGGG C^[CT]CG[GA]G Bsp1407I 543 TGTACA T^GTACA BspDI 751 ATCGAT AT^CGAT BspEI 506 TCCGGA T^CCGGA BspLU11I 841 ACATGT A^CATGT BsrBI 111 CCGCTC CCGCTC(-3/-3) BsrGI 543 TGTACA T^GTACA BstUI 1023 CGCG CG^CG BstXI 1113 CCAGGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1200 GGATCC [GA]^GATC[CT] CelII 682 GCTAAGC GC^T[ACGT]AGC CfoI 762 GCGC GCG^C ClaI 751 ATCGAT AT^CGAT DraI 1129 TTTAAA TTT^AAA EaeI 407 TGGCCA [CT]^GGCC[GA] EarI 310 GAAGAG CTCTTC(1/4) EcoRI 848 GAATTC G^AATTC HhaI 762 GCGC GCG^C HinP1I 762 GCGC G^CGC HincII 1039 GTCGAC GT[CT]^[GA]AC HindII 1039 GTCGAC GT[CT]^[GA]AC Ksp632I 310 GAAGAG CTCTTC(1/4) MaeII 1229 ACGT A^CGT MfeI 466 CAATTG C^AATTG MflI 1200 GGATCC [GA]^GATC[CT] MluNI 407 TGGCCA TGG^CCA MroI 506 TCCGGA T^CCGGA MscI 407 TGGCCA TGG^CCA MslI 10 CACGGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 58 CAGCTG C[AC]G^C[GT]G MunI 466 CAATTG C^AATTG MvnI 1023 CGCG CG^CG NcoI 1143 CCATGG C^CATGG PflMI 765 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1081 GAGTC GAGTC(4/5) PmaCI 1228 CACGTG CAC^GTG PmlI 1228 CACGTG CAC^GTG PpuMI 703 AGGTCCT [GA]G^G[AT]CC[CT] PstI 1221 CTGCAG CTGCA^G PvuII 58 CAGCTG CAG^CTG SalI 1039 GTCGAC G^TCGAC SapI 310 GAAGAGC GCTCTTC(1/4) SfaNI 139 GCATC GCATC(5/9) SfiI 571 GGCCTAGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 827 CCCGGG CCC^GGG SspBI 543 TGTACA T^GTACA Van91I 765 CCATGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1200 GGATCC [GA]^GATC[CT] XmaCI 827 CCCGGG C^CCGGG XmaI 827 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 562 GTAGAC GT^[AC][GT]AC 1039 GTCGAC AcsI 848 GAATTC [GA]^AATT[CT] 877 GAATTT AfaI 105 GTAC GT^AC 544 GTAC ApoI 848 GAATTC [GA]^AATT[CT] 877 GAATTT Asp700I 85 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 736 GAAAGTATTC BbsI 34 GAAGAC GAAGAC(2/6) 85 GAAGAC BglI 572 GCCTAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 777 GCCTGGGCGGC BpuAI 34 GAAGAC GAAGAC(2/6) 85 GAAGAC BseRI 496 GAGGAG GAGGAG(10/8) 1153 GAGGAG BsmAI 838 GAGAC GTCTC(1/5) 637 GTCTC Cac8I 581 GCCCGC GC[ACGT]^[ACGT]GC 678 GCCAGC DraII 703 AGGTCCT [GA]G^G[ACGT]CC[CT] 977 AGGGCCT DsaI 9 CCACGG C^C[GA][CT]GG 1143 CCATGG EcoNI 568 CCTGGCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 960 CCTCATACAGG EcoO109I 703 AGGTCCT [GA]G^G[ACGT]CC[CT] 977 AGGGCCT EcoT14I 573 CCTAGG C^C[AT][AT]GG 1143 CCATGG FauI 27 CCCGC CCCGC(4/6) 582 CCCGC NspI 171 ACATGC [GA]CATG^[CT] 841 ACATGT RsaI 105 GTAC GT^AC 544 GTAC SfcI 1215 CTGTAG C^T[GA][CT]AG 1221 CTGCAG StyI 573 CCTAGG C^C[AT][AT]GG 1143 CCATGG TfiI 999 GATTC G^A[AT]TC 1124 GATTC XmnI 85 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 736 GAAAGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BfaI BmyI BpmI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I