Restriction map for
pFN21AB6184
(Circular) (Six-base) MAPSORT of: pFN21AB6184.seq Check: 3202 from: 1 to: 6805
pFN21AB6184 6805 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3979 3979
Size: 6805
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4615 278
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4148 1937
Size: 2211 4594
Acc65I G'GTAC_C
Cuts at: 4126 4126
Size: 6805
AclI AA'CG_TT
Cuts at: 1563 4932 5305 1563
Size: 3369 373 3063
Fragments arranged by size:
3369 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2952 3213 3620 4088 4868 6252 6520
Size: 731 589 261 407 468 780 1384 268
Cuts at: 6520 1632
Size: 1917
Fragments arranged by size:
1917 1384 780 731 589 468 407 268
261
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6608
AflIII A'CryG_T
Cuts at: 1203 5704 6681 1203
Size: 4501 977 1327
Fragments arranged by size:
4501 1327 977
AgeI A'CCGG_T
Cuts at: 5636 5636
Size: 6805
AhdI GACnn_n'nnGTC
Cuts at: 5534 5534
Size: 6805
AleI CACnn'nnGTG
Cuts at: 1166 3434 1166
Size: 2268 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6805
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2153 2185 2153
Size: 32 6773
AlwNI CAG_nnn'CTG
Cuts at: 3373 3990 4057 4548 6120 3373
Size: 617 67 491 1572 4058
Fragments arranged by size:
4058 1572 617 491 67
ApaI G_GGCC'C
Cuts at: 2137 2137
Size: 6805
ApaLI G'TGCA_C
Cuts at: 4862 6018 4862
Size: 1156 5649
ApoI r'AATT_y
Cuts at: 1493 2819 2984 4114 4340 4492 6601 1493
Size: 1326 165 1130 226 152 2109 1697
Fragments arranged by size:
2109 1697 1326 1130 226 165 152
AseI AT'TA_AT
Cuts at: 160 1032 5359 160
Size: 872 4327 1606
Fragments arranged by size:
4327 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
AvaI C'yCGr_G
Cuts at: 1870 1943 2621 3698 3923 4130 1870
Size: 73 678 1077 225 207 4545
Fragments arranged by size:
4545 1077 678 225 207 73
BamHI G'GATC_C
Cuts at: 4135 4502 4135
Size: 367 6438
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3097 4126 5581 618
Size: 325 217 627 1310 1029 1455 1842
Fragments arranged by size:
1842 1455 1310 1029 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2137 2622 2772 2811 3206 3416 3737 4124
Size: 746 485 150 39 395 210 321 387
Cuts at: 4124 1391
Size: 4072
Fragments arranged by size:
4072 746 485 395 387 321 210 150
39
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2280 2666 3888 4001 928
Size: 971 381 386 1222 113 3732
Fragments arranged by size:
3732 1222 971 386 381 113
BbvCI CC'TCA_GC
Cuts at: 2129 2129
Size: 6805
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3178 3212 4996 5030 1903
Size: 34 1241 34 1784 34 3678
Fragments arranged by size:
3678 1784 1241 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4697 5907 4697
Size: 1210 5595
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6805
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3126 5416 136
Size: 107 122 71 631 605 1454 2290 1525
Fragments arranged by size:
2290 1525 1454 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2782 6800 2782
Size: 4018 2787
BlpI GC'TnA_GC
Cuts at: 4222 4222
Size: 6805
Bme1580I G_kGCm'C
Cuts at: 1790 2137 2650 3102 4866 6022 1790
Size: 347 513 452 1764 1156 2573
Fragments arranged by size:
2573 1764 1156 513 452 347
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6805
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2594 3096 3361
Size: 416 53 83 399 24 1177 502 265
Cuts at: 3361 3428 4536 5494 442
Size: 67 1108 958 1753
Fragments arranged by size:
1753 1177 1108 958 502 416 399 265
83 67 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6805
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3433 3433
Size: 6805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2624 2636 3428 3602 3647 5465 1239
Size: 1385 12 792 174 45 1818 2579
Fragments arranged by size:
2579 1818 1385 792 174 45 12
Bpu10I CC'TnA_GC
Cuts at: 2129 3146 2129
Size: 1017 5788
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2593 3218 4930 5795 6093 2593
Size: 625 1712 865 298 3305
Fragments arranged by size:
3305 1712 865 625 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5468 882
Size: 617 3969 2219
Fragments arranged by size:
3969 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4596 493
Size: 711 3392 2702
Fragments arranged by size:
3392 2702 711
BsaBI GATnn'nnATC
Cuts at: 3176 6799 3176
Size: 3623 3182
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4612 4994 5629 275
Size: 53 83 186 936 3079 382 635 1451
Fragments arranged by size:
3079 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3750 5238 5636 5910 6057 6468 3750
Size: 1488 398 274 147 411 4087
Fragments arranged by size:
4087 1488 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2675 2705 1178
Size: 30 165 30 1272 30 5278
Fragments arranged by size:
5278 1272 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2129 2438 2441 2729 2744 2891 3644 1186
Size: 943 309 3 288 15 147 753 4347
Fragments arranged by size:
4347 943 753 309 288 147 15 3
BseYI C'CCAG_C
Cuts at: 2353 2461 2472 2649 3185 4073 6008 6504 2353
Size: 108 11 177 536 888 1935 496 2654
Fragments arranged by size:
2654 1935 888 536 496 177 108 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2027 3059 3455 3460 3476 1163
Size: 573 291 1032 396 5 16 4492
Fragments arranged by size:
4492 1032 573 396 291 16 5
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4255 5016 6044 1540
Size: 275 186 2254 761 1028 2301
Fragments arranged by size:
2301 2254 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2622 3234 3335 4866 4951 6022 2622
Size: 612 101 1531 85 1071 3405
Fragments arranged by size:
3405 1531 1071 612 101 85
BsmI GAATG_Cn'
Cuts at: 4316 4409 4316
Size: 93 6712
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2622 2650 2772 2811 3102 3206
Size: 399 347 485 28 122 39 291 104
Cuts at: 3206 3234 3335 3416 3737 4124 4866 4951 6022
Size: 28 101 81 321 387 742 85 1071
Cuts at: 6022 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 485 399 387 347 321
291 122 104 101 85 81 39 28
28
BspEI T'CCGG_A
Cuts at: 3750 6468 3750
Size: 2718 4087
BspHI T'CATG_A
Cuts at: 4694 6424 4694
Size: 1730 5075
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3979 4160 844
Size: 3135 181 3489
Fragments arranged by size:
3489 3135 181
BsrBI CCG'CTC
Cuts at: 1394 1823 4692 1394
Size: 429 2869 3507
Fragments arranged by size:
3507 2869 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2213 5300 5474 65
Size: 1183 965 3087 174 1396
Fragments arranged by size:
3087 1396 1183 965 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2349 5449 5636 1700
Size: 110 539 3100 187 2869
Fragments arranged by size:
3100 2869 539 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6805
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6805
BssSI C'ACGA_G
Cuts at: 4865 5877 4865
Size: 1012 5793
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2442 3070 794
Size: 1648 628 4529
Fragments arranged by size:
4529 1648 628
BstBI TT'CG_AA
Cuts at: 5609 5609
Size: 6805
BstEII G'GTnAC_C
Cuts at: 1184 3457 1184
Size: 2273 4532
BstYI r'GATC_y
Cuts at: 1972 2029 2782 4135 4502 4887 4904 6356 6442
Size: 57 753 1353 367 385 17 1452 86
Cuts at: 6442 6454 6800 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1353 753 385 367 346 86
57 17 12
Bsu36I CC'TnA_GG
Cuts at: 2397 2997 2397
Size: 600 6205
BtgI C'CryG_G
Cuts at: 513 1065 2258 2655 3537 513
Size: 552 1193 397 882 3781
Fragments arranged by size:
3781 1193 882 552 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6066
Fragments arranged by size:
6066 683 56
BtsI GCAGTG_nn'
Cuts at: 2324 2369 2799 3467 3806 3942 4321 5115 5135
Size: 45 430 668 339 136 379 794 20
Cuts at: 5135 2324
Size: 3994
Fragments arranged by size:
3994 794 668 430 379 339 136 45
20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6770
DraI TTT'AAA
Cuts at: 4109 4109
Size: 6805
DraIII CAC_nnn'GTG
Cuts at: 2458 2458
Size: 6805
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2282 5812 809
Size: 507 966 3530 1802
Fragments arranged by size:
3530 1802 966 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 3127 4252
Size: 54 1425 50 275 18 345 952 1125
Cuts at: 4252 5141 6770 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1125 952 889 345 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4252 1537
Size: 275 2440 4090
Fragments arranged by size:
4090 2440 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3242 3704 3856 4735 1353
Size: 1889 462 152 879 3423
Fragments arranged by size:
3423 1889 879 462 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2029 3079 3745 5392 5766 5912
Size: 1064 138 440 1050 666 1647 374 146
Cuts at: 5912 387
Size: 1280
Fragments arranged by size:
1647 1280 1064 1050 666 440 374 146
138
EcoICRI GAG'CTC
Cuts at: 2620 2620
Size: 6805
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2624 2636 2952 3213 3428 3602
Size: 393 731 261 12 316 261 215 174
Cuts at: 3602 3620 3647 4088 4868 5465 6252 6520 1239
Size: 18 27 441 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 731 597 441 393 316
268 261 261 215 174 27 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 2249 2996 3629 2249
Size: 747 633 5425
Fragments arranged by size:
5425 747 633
EcoO109I rG'GnC_Cy
Cuts at: 2133 2134 2133
Size: 1 6804
EcoRI G'AATT_C
Cuts at: 2819 4114 4492 2819
Size: 1295 378 5132
Fragments arranged by size:
5132 1295 378
FspI TGC'GCA
Cuts at: 5311 5311
Size: 6805
HaeII r_GCGC'y
Cuts at: 1678 5952 1678
Size: 4274 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2990 3022 5446 5478 5520
Size: 32 135 32 1418 32 2424 32 42
Cuts at: 5520 5552 1373
Size: 32 2626
Fragments arranged by size:
2626 2424 1418 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4149 4395 669
Size: 988 281 2211 246 3079
Fragments arranged by size:
3079 2211 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6805
HpaI GTT'AAC
Cuts at: 4395 4395
Size: 6805
KpnI G_GTAC'C
Cuts at: 4130 4130
Size: 6805
MfeI C'AATT_G
Cuts at: 4404 4404
Size: 6805
MluI A'CGCG_T
Cuts at: 6681 6681
Size: 6805
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2934 5919 6103 1305
Size: 1629 2985 184 2007
Fragments arranged by size:
2985 2007 1629 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 3129 10
Size: 54 1425 343 345 952 3686
Fragments arranged by size:
3686 1425 952 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3434 4763 5122 5281 518
Size: 648 2268 1329 359 159 2042
Fragments arranged by size:
2268 2042 1329 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2472 2912 3043 4221 4898 6046 6291
Size: 522 344 440 131 1178 677 1148 245
Cuts at: 6291 1606
Size: 2120
Fragments arranged by size:
2120 1178 1148 677 522 440 344 245
131
NaeI GCC'GGC
Cuts at: 2351 2351
Size: 6805
NcoI C'CATG_G
Cuts at: 513 1065 2258 3537 513
Size: 552 1193 1279 3781
Fragments arranged by size:
3781 1279 1193 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6805
NgoMIV G'CCGG_C
Cuts at: 2349 2349
Size: 6805
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6805
NotI GC'GGCC_GC
Cuts at: 4252 4252
Size: 6805
NspI r_CATG'y
Cuts at: 1132 1229 3506 4163 5708 1132
Size: 97 2277 657 1545 2229
Fragments arranged by size:
2277 2229 1545 657 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6805
PciI A'CATG_T
Cuts at: 5704 5704
Size: 6805
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3451 3990 1108
Size: 2343 539 3923
Fragments arranged by size:
3923 2343 539
PfoI T'CCnGG_A
Cuts at: 3300 3300
Size: 6805
PmeI GTTT'AAAC
Cuts at: 4109 4109
Size: 6805
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4874 4906 6387 6419 4874
Size: 32 1481 32 5260
Fragments arranged by size:
5260 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6805
PsiI TTA'TAA
Cuts at: 4375 4375
Size: 6805
PspOMI G'GGCC_C
Cuts at: 2133 2133
Size: 6805
PspXI vC'TCGA_Gb
Cuts at: 2621 2621
Size: 6805
PstI C_TGCA'G
Cuts at: 830 3013 4157 830
Size: 2183 1144 3478
Fragments arranged by size:
3478 2183 1144
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6805
PvuII CAG'CTG
Cuts at: 2128 3043 2128
Size: 915 5890
SacI G_AGCT'C
Cuts at: 2622 2622
Size: 6805
SalI G'TCGA_C
Cuts at: 1936 4147 1936
Size: 2211 4594
SbfI CC_TGCA'GG
Cuts at: 4157 4157
Size: 6805
ScaI AGT'ACT
Cuts at: 3533 5053 6765 3533
Size: 1520 1712 3573
Fragments arranged by size:
3573 1712 1520
SfcI C'TryA_G
Cuts at: 826 1045 3009 4153 5288 5969 6160 826
Size: 219 1964 1144 1135 681 191 1471
Fragments arranged by size:
1964 1471 1144 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6805
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6805
SmaI CCC'GGG
Cuts at: 1872 4132 1872
Size: 2260 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2572 2621 3233 4909 5810 6072
Size: 197 926 629 49 612 1676 901 262
Cuts at: 6072 820
Size: 1553
Fragments arranged by size:
1676 1553 926 901 629 612 262 197
49
SnaBI TAC'GTA
Cuts at: 493 4596 493
Size: 4103 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6805
SphI G_CATG'C
Cuts at: 1132 4163 1132
Size: 3031 3774
SspI AAT'ATT
Cuts at: 5 52 4729 5
Size: 47 4677 2081
Fragments arranged by size:
4677 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6805
StyI C'CwwG_G
Cuts at: 513 1065 2258 2811 3537 4244 513
Size: 552 1193 553 726 707 3074
Fragments arranged by size:
3074 1193 726 707 553 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2435 2869 3125 4832 4849 5002 5187
Size: 1255 855 434 256 1707 17 153 185
Cuts at: 5187 5632 325
Size: 445 1498
Fragments arranged by size:
1707 1498 1255 855 445 434 256 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 2604 3531
Size: 274 80 33 51 1443 237 390 927
Cuts at: 3531 5051 6763 96
Size: 1520 1712 138
Fragments arranged by size:
1712 1520 1443 927 390 274 237 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2075 2107 3238 3270 6765 6797 2075
Size: 32 1131 32 3495 32 2083
Fragments arranged by size:
3495 2083 1131 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2246 1316
Size: 930 5875
XbaI T'CTAG_A
Cuts at: 4141 4141
Size: 6805
XhoI C'TCGA_G
Cuts at: 1943 2621 1943
Size: 678 6127
XmaI C'CCGG_G
Cuts at: 1870 4130 1870
Size: 2260 4545
XmnI GAAnn'nnTTC
Cuts at: 4934 4934
Size: 6805
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4613 276
Size: 53 83 186 936 3079 2468
Fragments arranged by size:
3079 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoRV FalI FseI FspAI KasI NarI
NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI