################################## # NO CUT ENZYMES ( 152 enzymes) # ################################## AatII AccII AccIII AciI AclI AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cac8I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 43 GGTACC G^GTACC AccI 983 GTCTAC GT^[AC][GT]AC AcsI 744 AAATTC [GA]^AATT[CT] AflIII 987 ACGTGT A^C[GA][CT]GT Alw44I 110 GTGCAC G^TGCAC AocI 216 CCTTAGG CC^T[ACGT]AGG ApaI 396 GGGCCC GGGCC^C ApaLI 110 GTGCAC G^TGCAC ApoI 744 AAATTC [GA]^AATT[CT] Asp718I 43 GGTACC G^GTACC AspHI 110 GTGCAC G[AT]GC[AT]^C AspI 384 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 51 TGGCCA TGG^CCA BbsI 959 GTCTTC GAAGAC(2/6) BpuAI 959 GTCTTC GAAGAC(2/6) BsaAI 986 TACGTG [CT]AC^GT[GA] BsaBI 934 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 604 GGTCTC GGTCTC(1/5) BsaWI 966 ACCGGA [AT]^CCGG[AT] BsiHKAI 110 GTGCAC G[AT]GC[AT]^C BsrDI 689 CATTGC GCAATG(2/0) BstAPI 693 GCAGTATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 407 CCAAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 216 CCTTAGG CC^T[ACGT]AGG DraI 538 TTTAAA TTT^AAA EaeI 51 TGGCCA [CT]^GGCC[GA] Eco81I 216 CCTTAGG CC^T[ACGT]AGG EcoT22I 686 ATGCAT ATGCA^T HapII 967 CCGG C^CGG HindIII 793 AAGCTT A^AGCTT HpaII 967 CCGG C^CGG HphI 100 TCACC GGTGA(8/7) KpnI 43 GGTACC GGTAC^C MaeII 987 ACGT A^CGT MamI 934 GATGGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 51 TGGCCA TGG^CCA MscI 51 TGGCCA TGG^CCA MspA1I 530 CAGCTG C[AC]G^C[GT]G MspI 967 CCGG C^CGG NlaIII 150 CATG CATG^ NsiI 686 ATGCAT ATGCA^T PflFI 384 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PvuII 530 CAGCTG CAG^CTG ScaI 56 AGTACT AGT^ACT StuI 251 AGGCCT AGG^CCT TaqI 557 TCGA T^CGA TfiI 200 GAATC G^A[AT]TC Tsp45I 1003 GTCAC ^GT[GC]AC Tth111I 384 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 557 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 386 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG 694 CAGTATCTG BanI 43 GGTACC G^G[CT][GA]CC 189 GGTGCC BanII 396 GGGCCC G[GA]GC[CT]^C 456 GGGCTC BfaI 289 CTAG C^TAG 304 CTAG BsaJI 764 CCCTGG C^C[ACGT][ACGT]GG 891 CCTGGG BsmAI 605 GTCTC GTCTC(1/5) 613 GTCTC EarI 22 CTCTTC CTCTTC(1/4) 617 CTCTTC Ksp632I 22 CTCTTC CTCTTC(1/4) 617 CTCTTC MaeI 289 CTAG C^TAG 304 CTAG MslI 113 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 238 CATATTGATG PleI 3 GAGTC GAGTC(4/5) 388 GAGTC PstI 274 CTGCAG CTGCA^G 424 CTGCAG SfaNI 520 GATGC GCATC(5/9) 944 GCATC SfcI 274 CTGCAG C^T[GA][CT]AG 424 CTGCAG SmlI 570 CTTGAG C^T[CT][GA]AG 856 CTTGAG TspRI 48 CAGTG CA[GC]TG[ACGT][ACGT]^ 277 CAGTG XbaI 288 TCTAGA T^CTAGA 303 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AfaI AluI AlwI BbvI BmyI BpmI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboI MboII MflI MnlI MseI MvaI MwoI NdeII NlaIV RsaI Sau3AI Sau96I ScrFI Tru9I TseI Tsp509I XhoII