(Circular) (Six-base) MAPSORT of: pFN21AE1404.seq Check: 6615 from: 1 to: 5719 pFN21AE1404 5719 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3529 278 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2989 3062 1937 Size: 1052 73 4594 Fragments arranged by size: 4594 1052 73 Acc65I G'GTAC_C Cuts at: 2048 3040 2048 Size: 992 4727 AclI AA'CG_TT Cuts at: 1563 3846 4219 1563 Size: 2283 373 3063 Fragments arranged by size: 3063 2283 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2378 3782 5166 5434 1632 Size: 746 1404 1384 268 1917 Fragments arranged by size: 1917 1404 1384 746 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5522 AflIII A'CryG_T Cuts at: 1203 2992 4618 5595 1203 Size: 1789 1626 977 1327 Fragments arranged by size: 1789 1626 1327 977 AgeI A'CCGG_T Cuts at: 4550 4550 Size: 5719 AhdI GACnn_n'nnGTC Cuts at: 4448 4448 Size: 5719 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5719 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5719 AlwNI CAG_nnn'CTG Cuts at: 2396 2704 3462 5034 2396 Size: 308 758 1572 3081 Fragments arranged by size: 3081 1572 758 308 ApaI G_GGCC'C Cuts at: 2405 2405 Size: 5719 ApaLI G'TGCA_C Cuts at: 2115 3776 4932 2115 Size: 1661 1156 2902 Fragments arranged by size: 2902 1661 1156 ApoI r'AATT_y Cuts at: 1493 2749 3028 3254 3406 5515 1493 Size: 1256 279 226 152 2109 1697 Fragments arranged by size: 2109 1697 1256 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 4273 160 Size: 872 3241 1606 Fragments arranged by size: 3241 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 AvaI C'yCGr_G Cuts at: 1870 1943 3044 1870 Size: 73 1101 4545 Fragments arranged by size: 4545 1101 73 BamHI G'GATC_C Cuts at: 3049 3416 3049 Size: 367 5352 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2194 3040 4495 618 Size: 325 217 627 261 146 846 1455 1842 Fragments arranged by size: 1842 1455 846 627 325 261 217 146 BanII G_rGCy'C Cuts at: 1391 2405 2465 3038 1391 Size: 1014 60 573 4072 Fragments arranged by size: 4072 1014 573 60 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2957 928 Size: 971 1058 3690 Fragments arranged by size: 3690 1058 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3910 3944 1903 Size: 34 1973 34 3678 Fragments arranged by size: 3678 1973 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3611 4821 3611 Size: 1210 4509 BclI T'GATC_A Cuts at: 1548 1548 Size: 5719 BfrBI ATG'CAT Cuts at: 2693 2693 Size: 5719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4330 136 Size: 107 122 71 631 605 2658 1525 Fragments arranged by size: 2658 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5714 5714 Size: 5719 BlpI GC'TnA_GC Cuts at: 3136 3136 Size: 5719 Bme1580I G_kGCm'C Cuts at: 1790 2119 2405 3780 4936 1790 Size: 329 286 1375 1156 2573 Fragments arranged by size: 2573 1375 1156 329 286 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5719 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3450 4408 442 Size: 416 53 83 399 24 2033 958 1753 Fragments arranged by size: 2033 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5719 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2441 2486 2867 4379 1239 Size: 1202 45 381 1512 2579 Fragments arranged by size: 2579 1512 1202 381 45 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2596 2882 3844 4709 5007 2596 Size: 286 962 865 298 3308 Fragments arranged by size: 3308 962 865 298 286 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2615 4382 882 Size: 617 1116 1767 2219 Fragments arranged by size: 2219 1767 1116 617 BsaAI yAC'GTr Cuts at: 493 1204 2993 3510 493 Size: 711 1789 517 2702 Fragments arranged by size: 2702 1789 711 517 BsaBI GATnn'nnATC Cuts at: 2943 5713 2943 Size: 2770 2949 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3526 3908 4543 275 Size: 53 83 186 936 1993 382 635 1451 Fragments arranged by size: 1993 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2971 4152 4550 4824 4971 5382 2971 Size: 1181 398 274 147 411 3308 Fragments arranged by size: 3308 1181 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5494 Fragments arranged by size: 5494 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5719 BseYI C'CCAG_C Cuts at: 2458 4922 5418 2458 Size: 2464 496 2759 Fragments arranged by size: 2759 2464 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5146 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3169 3930 4958 1540 Size: 275 186 1168 761 1028 2301 Fragments arranged by size: 2301 1168 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2119 3780 3865 4936 2119 Size: 1661 85 1071 2902 Fragments arranged by size: 2902 1661 1071 85 BsmI GAATG_Cn' Cuts at: 3230 3323 3230 Size: 93 5626 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2119 2405 2465 3038 3780 3865 4936 Size: 399 329 286 60 573 742 85 1071 Cuts at: 4936 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 573 399 329 286 85 60 BspEI T'CCGG_A Cuts at: 5382 5382 Size: 5719 BspHI T'CATG_A Cuts at: 3608 5338 3608 Size: 1730 3989 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3074 844 Size: 2230 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3606 1394 Size: 429 1783 3507 Fragments arranged by size: 3507 1783 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2693 4214 4388 65 Size: 1183 1445 1521 174 1396 Fragments arranged by size: 1521 1445 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4363 4550 1700 Size: 110 2553 187 2869 Fragments arranged by size: 2869 2553 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5719 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5719 BssSI C'ACGA_G Cuts at: 3779 4791 3779 Size: 1012 4707 BstAPI GCAn_nnn'nTGC Cuts at: 794 2704 794 Size: 1910 3809 BstBI TT'CG_AA Cuts at: 4523 4523 Size: 5719 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5719 BstXI CCAn_nnnn'nTGG Cuts at: 2419 2419 Size: 5719 BstYI r'GATC_y Cuts at: 1972 2425 2782 2892 3049 3416 3801 3818 5270 Size: 453 357 110 157 367 385 17 1452 Cuts at: 5270 5356 5368 5714 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 453 385 367 357 346 157 110 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2222 2222 Size: 5719 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5167 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4980 Fragments arranged by size: 4980 683 56 BtsI GCAGTG_nn' Cuts at: 2288 3235 4029 4049 2288 Size: 947 794 20 3958 Fragments arranged by size: 3958 947 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5684 DraI TTT'AAA Cuts at: 2545 3023 2545 Size: 478 5241 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4726 809 Size: 507 3410 1802 Fragments arranged by size: 3410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2056 3166 4055 Size: 54 1425 50 275 18 226 1110 889 Cuts at: 4055 5684 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1110 889 275 226 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3166 1537 Size: 275 1354 4090 Fragments arranged by size: 4090 1354 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2033 2628 3649 1353 Size: 680 595 1021 3423 Fragments arranged by size: 3423 1021 680 595 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4306 4680 4826 387 Size: 1064 138 2717 374 146 1280 Fragments arranged by size: 2717 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2441 2486 2867 3782 4379 5166 Size: 393 746 63 45 381 915 597 787 Cuts at: 5166 5434 1239 Size: 268 1524 Fragments arranged by size: 1524 915 787 746 597 393 381 268 63 45 EcoRI G'AATT_C Cuts at: 3028 3406 3028 Size: 378 5341 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3029 3029 Size: 5719 FspI TGC'GCA Cuts at: 4225 4225 Size: 5719 HaeII r_GCGC'y Cuts at: 1678 4866 1678 Size: 3188 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2569 2601 4360 4392 4434 Size: 32 135 32 997 32 1759 32 42 Cuts at: 4434 4466 1373 Size: 32 2626 Fragments arranged by size: 2626 1759 997 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3063 3309 669 Size: 988 281 1125 246 3079 Fragments arranged by size: 3079 1125 988 281 246 HindIII A'AGCT_T Cuts at: 748 2798 748 Size: 2050 3669 HpaI GTT'AAC Cuts at: 3309 3309 Size: 5719 KpnI G_GTAC'C Cuts at: 2052 3044 2052 Size: 992 4727 MfeI C'AATT_G Cuts at: 3318 3318 Size: 5719 MluI A'CGCG_T Cuts at: 5595 5595 Size: 5719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4833 5017 1305 Size: 3528 184 2007 Fragments arranged by size: 3528 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2058 10 Size: 54 1425 343 226 3671 Fragments arranged by size: 3671 1425 343 226 54 MslI CAynn'nnrTG Cuts at: 518 1166 2122 2247 3677 4036 4195 518 Size: 648 956 125 1430 359 159 2042 Fragments arranged by size: 2042 1430 956 648 359 159 125 MspA1I CmG'CkG Cuts at: 1606 2537 3135 3812 4960 5205 1606 Size: 931 598 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 598 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5167 NdeI CA'TA_TG Cuts at: 387 387 Size: 5719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5719 NotI GC'GGCC_GC Cuts at: 3166 3166 Size: 5719 NsiI A_TGCA'T Cuts at: 2695 2695 Size: 5719 NspI r_CATG'y Cuts at: 1132 1229 3077 4622 1132 Size: 97 1848 1545 2229 Fragments arranged by size: 2229 1848 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5719 PciI A'CATG_T Cuts at: 4618 4618 Size: 5719 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5719 PfoI T'CCnGG_A Cuts at: 2200 2200 Size: 5719 PmeI GTTT'AAAC Cuts at: 3023 3023 Size: 5719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3788 3820 5301 5333 3788 Size: 32 1481 32 4174 Fragments arranged by size: 4174 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5719 PsiI TTA'TAA Cuts at: 3289 3289 Size: 5719 PspOMI G'GGCC_C Cuts at: 2401 2401 Size: 5719 PstI C_TGCA'G Cuts at: 830 2283 2433 3071 830 Size: 1453 150 638 3478 Fragments arranged by size: 3478 1453 638 150 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5719 PvuII CAG'CTG Cuts at: 2537 2537 Size: 5719 SalI G'TCGA_C Cuts at: 1936 3061 1936 Size: 1125 4594 SbfI CC_TGCA'GG Cuts at: 3071 3071 Size: 5719 ScaI AGT'ACT Cuts at: 2063 3967 5679 2063 Size: 1904 1712 2103 Fragments arranged by size: 2103 1904 1712 SfcI C'TryA_G Cuts at: 826 1045 2279 2429 3067 4202 4883 5074 826 Size: 219 1234 150 638 1135 681 191 1471 Fragments arranged by size: 1471 1234 1135 681 638 219 191 150 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5719 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5719 SmaI CCC'GGG Cuts at: 1872 3046 1872 Size: 1174 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2575 2861 3823 4724 4986 820 Size: 197 926 632 286 962 901 262 1553 Fragments arranged by size: 1553 962 926 901 632 286 262 197 SnaBI TAC'GTA Cuts at: 493 3510 493 Size: 3017 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5719 SphI G_CATG'C Cuts at: 1132 3077 1132 Size: 1945 3774 SspI AAT'ATT Cuts at: 5 52 3643 5 Size: 47 3591 2081 Fragments arranged by size: 3591 2081 47 StuI AGG'CCT Cuts at: 1517 2258 1517 Size: 741 4978 StyI C'CwwG_G Cuts at: 513 1065 3158 513 Size: 552 2093 3074 Fragments arranged by size: 3074 2093 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3746 3763 3916 4101 4546 325 Size: 1255 2166 17 153 185 445 1498 Fragments arranged by size: 2166 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2061 2157 3965 Size: 274 80 33 51 1443 84 96 1808 Cuts at: 3965 5677 96 Size: 1712 138 Fragments arranged by size: 1808 1712 1443 274 138 96 84 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5679 5711 5679 Size: 32 5687 Tth111I GACn'n_nGTC Cuts at: 1316 2392 1316 Size: 1076 4643 XbaI T'CTAG_A Cuts at: 2293 2308 3055 2293 Size: 15 747 4957 Fragments arranged by size: 4957 747 15 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5719 XmaI C'CCGG_G Cuts at: 1870 3044 1870 Size: 1174 4545 XmnI GAAnn'nnTTC Cuts at: 3848 3848 Size: 5719 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3527 276 Size: 53 83 186 936 1993 2468 Fragments arranged by size: 2468 1993 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI