Restriction map for
pFN21AE4314
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# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI Hin1I HpaI KasI KpnI
KspI MamI MluI MroI MseI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Van91I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcsI 853 GAATTC [GA]^AATT[CT]
AfaI 549 GTAC GT^AC
AflIII 156 ACATGT A^C[GA][CT]GT
AlwNI 356 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 853 GAATTC [GA]^AATT[CT]
AspI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 658 GGATCC G^GATCC
BanI 64 GGCACC G^G[CT][GA]CC
BanII 541 GGGCTC G[GA]GC[CT]^C
BbsI 229 GAAGAC GAAGAC(2/6)
BfaI 103 CTAG C^TAG
BpmI 517 CTGGAG CTGGAG(16/14)
BpuAI 229 GAAGAC GAAGAC(2/6)
BspLU11I 156 ACATGT A^CATGT
BstBI 6 TTCGAA TT^CGAA
DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 222 GAAGAG CTCTTC(1/4)
EcoNI 293 CCTTGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRI 853 GAATTC G^AATTC
HindIII 250 AAGCTT A^AGCTT
HphI 373 GGTGA GGTGA(8/7)
Ksp632I 222 GAAGAG CTCTTC(1/4)
MaeI 103 CTAG C^TAG
MaeII 839 ACGT A^CGT
MfeI 319 CAATTG C^AATTG
MslI 486 CATCGGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 778 CAGCTG C[AC]G^C[GT]G
MunI 319 CAATTG C^AATTG
NspI 156 ACATGT [GA]CATG^[CT]
NspV 6 TTCGAA TT^CGAA
PflFI 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT]
PvuII 778 CAGCTG CAG^CTG
RsaI 549 GTAC GT^AC
SexAI 566 ACCAGGT A^CC[AT]GGT
SfuI 6 TTCGAA TT^CGAA
SmlI 544 CTCAAG C^T[CT][GA]AG
Tth111I 712 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 102 TCTAGA T^CTAGA
XcmI 777 CCAGCTGCAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AspHI 650 GTGCTC G[AT]GC[AT]^C
700 GTGCTC
BcnI 131 CCGGG CC^[GC]GG
696 CCCGG
BseRI 172 GAGGAG GAGGAG(10/8)
871 GAGGAG
BsgI 152 CTGCAC GTGCAG(16/14)
841 GTGCAG
BsiHKAI 650 GTGCTC G[AT]GC[AT]^C
700 GTGCTC
BsiYI 49 CCTGTCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
293 CCTTGGAAAGG
BslI 49 CCTGTCCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
293 CCTTGGAAAGG
BspMI 177 GCAGGT ACCTGC(4/8)
843 GCAGGT
BstF5I 192 CATCC GGATG(2/0)
580 CATCC
BstYI 426 GGATCT [GA]^GATC[CT]
658 GGATCC
Cac8I 75 GCCTGC GC[ACGT]^[ACGT]GC
934 GCTGGC
DsaI 449 CCATGG C^C[GA][CT]GG
676 CCATGG
FokI 192 CATCC GGATG(9/13)
580 CATCC
HapII 131 CCGG C^CGG
697 CCGG
HincII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HindII 331 GTTGAC GT[CT]^[GA]AC
861 GTTGAC
HinfI 4 GATTC G^A[ACGT]TC
148 GATTC
HpaII 131 CCGG C^CGG
697 CCGG
MaeIII 38 GTCAC ^GT[ACGT]AC
303 GTCAC
MflI 426 GGATCT [GA]^GATC[CT]
658 GGATCC
MspI 131 CCGG C^CGG
697 CCGG
NciI 131 CCGGG CC^[GC]GG
696 CCCGG
NcoI 449 CCATGG C^CATGG
676 CCATGG
PstI 77 CTGCAG CTGCA^G
781 CTGCAG
SfaNI 466 GATGC GCATC(5/9)
827 GCATC
TfiI 4 GATTC G^A[AT]TC
148 GATTC
Tsp45I 38 GTCAC ^GT[GC]AC
303 GTCAC
XhoII 426 GGATCT [GA]^GATC[CT]
658 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AlwI AvaII BalI BbvI
BmyI BsaJI Bsp1286I BsrI BssKI
BstNI CfoI Cfr13I DpnII EaeI
EcoRII EcoT14I Fnu4HI HaeIII HhaI
HinP1I ItaI MboI MboII MluNI
MnlI MscI MvaI MwoI NdeII
NlaIII NlaIV Sau3AI Sau96I ScrFI
SfcI StyI TaqI TseI Tsp509I
TspRI TthHB8I