################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI AscI Asp700I AspEI AviII AvrII BamHI BanII BbeI BbrPI BbsI BcgI BfrI BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmBI Bsp1407I BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I CpoI DraI DraIII Eam1105I Eco47III EcoNI EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NgoMIV NheI NruI NsiI NspV PacI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 66 enzymes) # ################################### Acc65I 625 GGTACC G^GTACC AfaI 626 GTAC GT^AC AocI 938 CCTCAGG CC^T[ACGT]AGG AseI 213 ATTAAT AT^TAAT AsnI 213 ATTAAT AT^TAAT Asp718I 625 GGTACC G^GTACC AspI 115 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1662 TGGCCA TGG^CCA BbvCI 916 GCTGAGG CCTCAGC(-5/-2) BciVI 1038 GTATCC GTATCC(6/5) BclI 1902 TGATCA T^GATCA BglI 10 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 704 AGATCT A^GATCT BlpI 43 GCTGAGC GC^T[ACGT]AGC Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC BsaBI 212 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 5 CGGCCG CG[GA][CT]^CG BsmAI 551 GAGAC GTCTC(1/5) BsmI 1064 GAATGC GAATGC(1/-1) BspDI 1045 ATCGAT AT^CGAT BssSI 1751 CTCGTG CACGAG(-5/-1) BstEII 561 GGTCACC G^GT[ACGT]ACC BstPI 561 GGTCACC G^GT[ACGT]ACC BstXI 376 CCATTTAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 938 CCTCAGG CC^T[ACGT]AGG CelII 43 GCTGAGC GC^T[ACGT]AGC CfoI 1310 GCGC GCG^C ClaI 1045 ATCGAT AT^CGAT DraII 1424 GGGACCT [GA]G^G[ACGT]CC[CT] DrdI 553 GACATCATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 5 CGGCCG C^GGCCG EclXI 5 CGGCCG C^GGCCG Eco52I 5 CGGCCG C^GGCCG Eco81I 938 CCTCAGG CC^T[ACGT]AGG EcoO109I 1424 GGGACCT [GA]G^G[ACGT]CC[CT] EcoO65I 561 GGTCACC G^GT[ACGT]ACC FbaI 1902 TGATCA T^GATCA HapII 1656 CCGG C^CGG HhaI 1310 GCGC GCG^C HinP1I 1310 GCGC G^CGC HincII 805 GTTGAC GT[CT]^[GA]AC HindII 805 GTTGAC GT[CT]^[GA]AC HpaII 1656 CCGG C^CGG KpnI 625 GGTACC GGTAC^C MamI 212 GATTAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1662 TGGCCA TGG^CCA MscI 1662 TGGCCA TGG^CCA MspI 1656 CCGG C^CGG NdeI 440 CATATG CA^TATG NotI 4 GCGGCCGC GC^GGCCGC NspI 1462 GCATGC [GA]CATG^[CT] PaeR7I 1397 CTCGAG C^TCGAG PflFI 115 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 1424 GGGACCT [GA]G^G[AT]CC[CT] PshBI 213 ATTAAT AT^TAAT PstI 1598 CTGCAG CTGCA^G RsaI 626 GTAC GT^AC SmaI 1655 CCCGGG CCC^GGG SmlI 1397 CTCGAG C^T[CT][GA]AG SphI 1462 GCATGC GCATG^C SspI 57 AATATT AAT^ATT StuI 935 AGGCCT AGG^CCT Tth111I 115 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 1397 CTCGAG C^TCGAG XmaCI 1655 CCCGGG C^CCGGG XmaI 1655 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AvaI 1397 CTCGAG C^[CT]CG[GA]G 1655 CCCGGG BcnI 1655 CCCGG CC^[GC]GG 1656 CCGGG BfaI 518 CTAG C^TAG 607 CTAG BsiYI 1206 CCCAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1771 CCCCAGTTTGG BslI 1206 CCCAGAGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 1771 CCCCAGTTTGG BsoBI 1397 CTCGAG C^[CT]CG[GA]G 1655 CCCGGG BspMI 1883 ACCTGC ACCTGC(4/8) 1548 GCAGGT DsaI 797 CCATGG C^C[GA][CT]GG 1847 CCATGG EarI 1978 CTCTTC CTCTTC(1/4) 889 GAAGAG FauI 1422 GCGGG CCCGC(4/6) 1473 GCGGG Ksp632I 1978 CTCTTC CTCTTC(1/4) 889 GAAGAG MaeI 518 CTAG C^TAG 607 CTAG MaeII 849 ACGT A^CGT 1907 ACGT MslI 1029 CACTGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1261 CACAAAGATG NciI 1655 CCCGG CC^[GC]GG 1656 CCGGG NcoI 797 CCATGG C^CATGG 1847 CCATGG PvuII 41 CAGCTG CAG^CTG 289 CAGCTG SfaNI 265 GATGC GCATC(5/9) 1603 GCATC XcmI 1074 CCAACGATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1723 CCAAAAGCTCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AcsI AluI AlwI ApoI AspHI AvaII BanI BbvI BmyI BpmI BsaJI BseRI BsiHKAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII