(Circular) (Six-base) MAPSORT of: pFN21AE4555.seq Check: 7758 from: 1 to: 6730 pFN21AE4555 6730 bp With 182 enzymes: SgfI * March 28, 2012 14:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4540 278 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4073 1937 Size: 2136 4594 Acc65I G'GTAC_C Cuts at: 2630 4051 2630 Size: 1421 5309 AclI AA'CG_TT Cuts at: 1563 4857 5230 1563 Size: 3294 373 3063 Fragments arranged by size: 3294 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4793 6177 6445 1632 Size: 3161 1384 268 1917 Fragments arranged by size: 3161 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6533 AflIII A'CryG_T Cuts at: 1203 5629 6606 1203 Size: 4426 977 1327 Fragments arranged by size: 4426 1327 977 AgeI A'CCGG_T Cuts at: 5561 5561 Size: 6730 AhdI GACnn_n'nnGTC Cuts at: 5459 5459 Size: 6730 AleI CACnn'nnGTG Cuts at: 1166 3038 1166 Size: 1872 4858 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6730 AlwNI CAG_nnn'CTG Cuts at: 4473 6045 4473 Size: 1572 5158 ApaLI G'TGCA_C Cuts at: 4787 5943 4787 Size: 1156 5574 ApoI r'AATT_y Cuts at: 1493 2099 2234 2399 2453 2783 3257 3297 3962 Size: 606 135 165 54 330 474 40 665 Cuts at: 3962 4039 4265 4417 6526 1493 Size: 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 665 606 474 330 226 165 152 135 77 54 40 AseI AT'TA_AT Cuts at: 160 1032 2219 5284 160 Size: 872 1187 3065 1606 Fragments arranged by size: 3065 1606 1187 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 AvaI C'yCGr_G Cuts at: 1870 1943 3402 3660 4055 1870 Size: 73 1459 258 395 4545 Fragments arranged by size: 4545 1459 395 258 73 BamHI G'GATC_C Cuts at: 4060 4427 4060 Size: 367 6363 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2630 2729 3458 3584 4051 Size: 325 217 627 843 99 729 126 467 Cuts at: 4051 5506 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 843 729 627 467 325 217 126 99 BanII G_rGCy'C Cuts at: 1391 4049 1391 Size: 2658 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5759 BbvCI CC'TCA_GC Cuts at: 2922 2922 Size: 6730 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4921 4955 1903 Size: 34 2984 34 3678 Fragments arranged by size: 3678 2984 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3054 4622 5832 3054 Size: 1568 1210 3952 Fragments arranged by size: 3952 1568 1210 BclI T'GATC_A Cuts at: 1548 3907 1548 Size: 2359 4371 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2021 5341 Size: 107 122 71 631 605 337 12 3320 Cuts at: 5341 136 Size: 1525 Fragments arranged by size: 3320 1525 631 605 337 122 107 71 12 BglII A'GATC_T Cuts at: 2709 6725 2709 Size: 4016 2714 BlpI GC'TnA_GC Cuts at: 2049 4147 2049 Size: 2098 4632 Bme1580I G_kGCm'C Cuts at: 1790 2266 4791 5947 1790 Size: 476 2525 1156 2573 Fragments arranged by size: 2573 2525 1156 476 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6730 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2398 2538 3772 Size: 416 53 83 399 24 981 140 1234 Cuts at: 3772 4022 4461 5419 442 Size: 250 439 958 1753 Fragments arranged by size: 1753 1234 981 958 439 416 399 250 140 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6730 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2504 2646 3039 3327 3339 5390 1239 Size: 1265 142 393 288 12 2051 2579 Fragments arranged by size: 2579 2051 1265 393 288 142 12 Bpu10I CC'TnA_GC Cuts at: 2863 2922 2863 Size: 59 6671 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4855 5720 6018 4855 Size: 865 298 5567 Fragments arranged by size: 5567 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5393 882 Size: 617 3894 2219 Fragments arranged by size: 3894 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4521 493 Size: 711 3317 2702 Fragments arranged by size: 3317 2702 711 BsaBI GATnn'nnATC Cuts at: 2221 6724 2221 Size: 4503 2227 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4537 4919 5554 275 Size: 53 83 186 936 3004 382 635 1451 Fragments arranged by size: 3004 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5163 5561 5835 5982 6393 5163 Size: 398 274 147 411 5500 Fragments arranged by size: 5500 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4014 4044 1178 Size: 30 165 30 2611 30 3864 Fragments arranged by size: 3864 2611 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2774 2877 3855 1186 Size: 1588 103 978 4061 Fragments arranged by size: 4061 1588 978 103 BseYI C'CCAG_C Cuts at: 5933 6429 5933 Size: 496 6234 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6157 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2013 4180 4941 5969 1540 Size: 275 186 12 2167 761 1028 2301 Fragments arranged by size: 2301 2167 1028 761 275 186 12 BsiHKAI G_wGCw'C Cuts at: 2490 3415 3757 4791 4876 5947 2490 Size: 925 342 1034 85 1071 3273 Fragments arranged by size: 3273 1071 1034 925 342 85 BsmI GAATG_Cn' Cuts at: 3075 4241 4334 3075 Size: 1166 93 5471 Fragments arranged by size: 5471 1166 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2266 2490 3415 3757 4049 4791 4876 Size: 399 476 224 925 342 292 742 85 Cuts at: 4876 5947 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 925 742 476 399 342 292 224 85 BspEI T'CCGG_A Cuts at: 6393 6393 Size: 6730 BspHI T'CATG_A Cuts at: 4619 6349 4619 Size: 1730 5000 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3544 3897 4085 844 Size: 2700 353 188 3489 Fragments arranged by size: 3489 2700 353 188 BsrBI CCG'CTC Cuts at: 1394 1823 4617 1394 Size: 429 2794 3507 Fragments arranged by size: 3507 2794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5225 5399 65 Size: 1183 3977 174 1396 Fragments arranged by size: 3977 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5374 5561 1700 Size: 110 3564 187 2869 Fragments arranged by size: 3564 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6730 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6730 BssSI C'ACGA_G Cuts at: 3756 4790 5802 3756 Size: 1034 1012 4684 Fragments arranged by size: 4684 1034 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6730 BstBI TT'CG_AA Cuts at: 5534 5534 Size: 6730 BstEII G'GTnAC_C Cuts at: 1184 2566 1184 Size: 1382 5348 BstXI CCAn_nnnn'nTGG Cuts at: 2388 2388 Size: 6730 BstYI r'GATC_y Cuts at: 1972 2041 2709 3363 3447 4060 4427 4812 4829 Size: 69 668 654 84 613 367 385 17 Cuts at: 4829 6281 6367 6379 6725 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 668 654 613 385 367 346 86 84 69 17 12 Bsu36I CC'TnA_GG Cuts at: 2944 2944 Size: 6730 BtgI C'CryG_G Cuts at: 513 1065 2004 2802 3852 513 Size: 552 939 798 1050 3391 Fragments arranged by size: 3391 1050 939 798 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3832 487 Size: 683 56 2606 3385 Fragments arranged by size: 3385 2606 683 56 BtsI GCAGTG_nn' Cuts at: 3033 4246 5040 5060 3033 Size: 1213 794 20 4703 Fragments arranged by size: 4703 1213 794 20 ClaI AT'CG_AT Cuts at: 1554 3051 1554 Size: 1497 5233 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2157 2192 3180 3215 529 Size: 35 1593 35 988 35 4044 Fragments arranged by size: 4044 1593 988 35 35 35 DraI TTT'AAA Cuts at: 4034 4034 Size: 6730 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2564 5737 809 Size: 507 1248 3173 1802 Fragments arranged by size: 3173 1802 1248 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2010 3495 3667 Size: 54 1425 50 275 18 180 1485 172 Cuts at: 3667 4177 5066 6695 8 Size: 510 889 1629 43 Fragments arranged by size: 1629 1485 1425 889 510 275 180 172 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2010 4177 1537 Size: 275 198 2167 4090 Fragments arranged by size: 4090 2167 275 198 EarI CTCTTCn'nnn_ Cuts at: 1353 2889 3989 4660 1353 Size: 1536 1100 671 3423 Fragments arranged by size: 3423 1536 1100 671 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2564 5317 5691 5837 387 Size: 1064 138 975 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 975 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2504 2646 3039 3327 3339 4793 5390 Size: 393 872 142 393 288 12 1454 597 Cuts at: 5390 6177 6445 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1454 872 787 597 393 393 288 268 142 12 EcoO109I rG'GnC_Cy Cuts at: 3430 3430 Size: 6730 EcoRI G'AATT_C Cuts at: 4039 4417 4039 Size: 378 6352 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2518 3550 2518 Size: 1032 5698 FspI TGC'GCA Cuts at: 5236 5236 Size: 6730 HaeII r_GCGC'y Cuts at: 1678 5877 1678 Size: 4199 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2026 2058 2872 2904 5371 Size: 32 135 32 454 32 814 32 2467 Cuts at: 5371 5403 5445 5477 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2467 814 454 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2812 4074 4320 669 Size: 988 281 874 1262 246 3079 Fragments arranged by size: 3079 1262 988 874 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6730 HpaI GTT'AAC Cuts at: 4320 4320 Size: 6730 KpnI G_GTAC'C Cuts at: 2634 4055 2634 Size: 1421 5309 MfeI C'AATT_G Cuts at: 4329 4329 Size: 6730 MluI A'CGCG_T Cuts at: 6606 6606 Size: 6730 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3007 5844 6028 1305 Size: 1702 2837 184 2007 Fragments arranged by size: 2837 2007 1702 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3669 10 Size: 54 1425 343 1837 3071 Fragments arranged by size: 3071 1837 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3038 3270 4688 5047 5206 518 Size: 648 1872 232 1418 359 159 2042 Fragments arranged by size: 2042 1872 1418 648 359 232 159 MspA1I CmG'CkG Cuts at: 1606 2048 2296 3684 4146 4823 5971 6216 1606 Size: 442 248 1388 462 677 1148 245 2120 Fragments arranged by size: 2120 1388 1148 677 462 442 248 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2802 3852 513 Size: 552 939 798 1050 3391 Fragments arranged by size: 3391 1050 939 798 552 NdeI CA'TA_TG Cuts at: 387 2446 387 Size: 2059 4671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6730 NotI GC'GGCC_GC Cuts at: 2010 4177 2010 Size: 2167 4563 NspI r_CATG'y Cuts at: 1132 1229 3471 4088 5633 1132 Size: 97 2242 617 1545 2229 Fragments arranged by size: 2242 2229 1545 617 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6730 PciI A'CATG_T Cuts at: 5629 5629 Size: 6730 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6730 PfoI T'CCnGG_A Cuts at: 3878 3878 Size: 6730 PmeI GTTT'AAAC Cuts at: 4034 4034 Size: 6730 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4799 4831 6312 6344 4799 Size: 32 1481 32 5185 Fragments arranged by size: 5185 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3430 3430 Size: 6730 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6730 PsiI TTA'TAA Cuts at: 4300 4300 Size: 6730 PspXI vC'TCGA_Gb Cuts at: 3402 3402 Size: 6730 PstI C_TGCA'G Cuts at: 830 3607 4082 830 Size: 2777 475 3478 Fragments arranged by size: 3478 2777 475 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6730 PvuII CAG'CTG Cuts at: 2048 2296 2048 Size: 248 6482 SalI G'TCGA_C Cuts at: 1936 4072 1936 Size: 2136 4594 SbfI CC_TGCA'GG Cuts at: 4082 4082 Size: 6730 ScaI AGT'ACT Cuts at: 4978 6690 4978 Size: 1712 5018 SfcI C'TryA_G Cuts at: 826 1045 2713 2992 3603 3812 4078 5213 5894 Size: 219 1668 279 611 209 266 1135 681 Cuts at: 5894 6085 826 Size: 191 1471 Fragments arranged by size: 1668 1471 1135 681 611 279 266 219 209 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6730 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6730 SmaI CCC'GGG Cuts at: 1872 3662 4057 1872 Size: 1790 395 4545 Fragments arranged by size: 4545 1790 395 SmlI C'TyrA_G Cuts at: 820 1017 1943 3402 4834 5735 5997 820 Size: 197 926 1459 1432 901 262 1553 Fragments arranged by size: 1553 1459 1432 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4521 493 Size: 4028 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6730 SphI G_CATG'C Cuts at: 1132 3471 4088 1132 Size: 2339 617 3774 Fragments arranged by size: 3774 2339 617 SrfI GCCC'GGGC Cuts at: 3662 3662 Size: 6730 SspI AAT'ATT Cuts at: 5 52 2064 4654 5 Size: 47 2012 2590 2081 Fragments arranged by size: 2590 2081 2012 47 StuI AGG'CCT Cuts at: 1517 2942 1517 Size: 1425 5305 StyI C'CwwG_G Cuts at: 513 1065 2004 2016 2802 2846 3852 4169 513 Size: 552 939 12 786 44 1006 317 3074 Fragments arranged by size: 3074 1006 939 786 552 317 44 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2815 3268 4757 4774 4927 5112 5557 Size: 1255 1235 453 1489 17 153 185 445 Cuts at: 5557 325 Size: 1498 Fragments arranged by size: 1498 1489 1255 1235 453 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4976 6688 96 Size: 274 80 33 51 1443 2999 1712 138 Fragments arranged by size: 2999 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3382 3414 6690 6722 3382 Size: 32 3276 32 3390 Fragments arranged by size: 3390 3276 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2123 1316 Size: 807 5923 XbaI T'CTAG_A Cuts at: 4066 4066 Size: 6730 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3086 3735 3086 Size: 649 6081 XhoI C'TCGA_G Cuts at: 1943 3402 1943 Size: 1459 5271 XmaI C'CCGG_G Cuts at: 1870 3660 4055 1870 Size: 1790 395 4545 Fragments arranged by size: 4545 1790 395 XmnI GAAnn'nnTTC Cuts at: 4859 4859 Size: 6730 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4538 276 Size: 53 83 186 936 3004 2468 Fragments arranged by size: 3004 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SwaI