(Circular) (Six-base) MAPSORT of: pFN21AB5920.seq Check: 760 from: 1 to: 6301 pFN21AB5920 6301 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2354 2826 2993 3432 2354 Size: 472 167 439 5223 Fragments arranged by size: 5223 472 439 167 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4111 278 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3357 3644 1937 Size: 1420 287 4594 Fragments arranged by size: 4594 1420 287 Acc65I G'GTAC_C Cuts at: 3622 3622 Size: 6301 AclI AA'CG_TT Cuts at: 1563 4428 4801 1563 Size: 2865 373 3063 Fragments arranged by size: 3063 2865 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3466 4364 5748 6016 1632 Size: 1834 898 1384 268 1917 Fragments arranged by size: 1917 1834 1384 898 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6104 AflIII A'CryG_T Cuts at: 1203 2382 2850 5200 6177 1203 Size: 1179 468 2350 977 1327 Fragments arranged by size: 2350 1327 1179 977 468 AgeI A'CCGG_T Cuts at: 5132 5132 Size: 6301 AhdI GACnn_n'nnGTC Cuts at: 2488 5030 2488 Size: 2542 3759 AleI CACnn'nnGTG Cuts at: 1166 2385 3590 1166 Size: 1219 1205 3877 Fragments arranged by size: 3877 1219 1205 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6301 AlwNI CAG_nnn'CTG Cuts at: 2066 4044 5616 2066 Size: 1978 1572 2751 Fragments arranged by size: 2751 1978 1572 ApaI G_GGCC'C Cuts at: 2264 2264 Size: 6301 ApaLI G'TGCA_C Cuts at: 4358 5514 4358 Size: 1156 5145 ApoI r'AATT_y Cuts at: 1493 3610 3836 3988 6097 1493 Size: 2117 226 152 2109 1697 Fragments arranged by size: 2117 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4855 160 Size: 872 3823 1606 Fragments arranged by size: 3823 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 AvaI C'yCGr_G Cuts at: 1870 1943 2255 3168 3626 1870 Size: 73 312 913 458 4545 Fragments arranged by size: 4545 913 458 312 73 BamHI G'GATC_C Cuts at: 2806 3631 3998 2806 Size: 825 367 5109 Fragments arranged by size: 5109 825 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2366 2795 2814 3622 5077 Size: 325 217 627 579 429 19 808 1455 Cuts at: 5077 618 Size: 1842 Fragments arranged by size: 1842 1455 808 627 579 429 325 217 19 BanII G_rGCy'C Cuts at: 1391 2226 2249 2264 2410 2585 3086 3620 1391 Size: 835 23 15 146 175 501 534 4072 Fragments arranged by size: 4072 835 534 501 175 146 23 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2055 2536 2661 3224 928 Size: 971 156 481 125 563 4005 Fragments arranged by size: 4005 971 563 481 156 125 BbvCI CC'TCA_GC Cuts at: 3233 3233 Size: 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2800 2834 4492 4526 1903 Size: 34 863 34 1658 34 3678 Fragments arranged by size: 3678 1658 863 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4193 5403 4193 Size: 1210 5091 BclI T'GATC_A Cuts at: 1548 1548 Size: 6301 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2772 4912 136 Size: 107 122 71 631 605 1100 2140 1525 Fragments arranged by size: 2140 1525 1100 631 605 122 107 71 BglII A'GATC_T Cuts at: 6296 6296 Size: 6301 BlpI GC'TnA_GC Cuts at: 3718 3718 Size: 6301 Bme1580I G_kGCm'C Cuts at: 1790 2264 2798 4362 5518 1790 Size: 474 534 1564 1156 2573 Fragments arranged by size: 2573 1564 1156 534 474 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6301 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4032 4990 442 Size: 416 53 83 399 24 2615 958 1753 Fragments arranged by size: 2615 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2251 2486 2602 3212 3229 4961 1239 Size: 1012 235 116 610 17 1732 2579 Fragments arranged by size: 2579 1732 1012 610 235 116 17 Bpu10I CC'TnA_GC Cuts at: 2220 3233 2220 Size: 1013 5288 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2437 3115 4426 5291 5589 2437 Size: 678 1311 865 298 3149 Fragments arranged by size: 3149 1311 865 678 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2420 2620 4964 882 Size: 617 921 200 2344 2219 Fragments arranged by size: 2344 2219 921 617 200 BsaAI yAC'GTr Cuts at: 493 1204 2383 2827 2857 4092 493 Size: 711 1179 444 30 1235 2702 Fragments arranged by size: 2702 1235 1179 711 444 30 BsaBI GATnn'nnATC Cuts at: 6295 6295 Size: 6301 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4108 4490 5125 275 Size: 53 83 186 936 2575 382 635 1451 Fragments arranged by size: 2575 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2044 2547 4734 5132 5406 5553 5964 2044 Size: 503 2187 398 274 147 411 2381 Fragments arranged by size: 2381 2187 503 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6076 Fragments arranged by size: 6076 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2129 2186 2189 3209 3335 3476 3479 1186 Size: 943 57 3 1020 126 141 3 4008 Fragments arranged by size: 4008 1020 943 141 126 57 3 3 BseYI C'CCAG_C Cuts at: 2059 2130 3146 5504 6000 2059 Size: 71 1016 2358 496 2360 Fragments arranged by size: 2360 2358 1016 496 71 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2420 2525 3044 3291 3568 1163 Size: 573 684 105 519 247 277 3896 Fragments arranged by size: 3896 684 573 519 277 247 105 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3751 4512 5540 1540 Size: 275 186 1750 761 1028 2301 Fragments arranged by size: 2301 1750 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2410 2585 4362 4447 5518 2226 Size: 184 175 1777 85 1071 3009 Fragments arranged by size: 3009 1777 1071 184 175 85 BsmI GAATG_Cn' Cuts at: 2683 3812 3905 2683 Size: 1129 93 5079 Fragments arranged by size: 5079 1129 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2249 2264 2410 2585 2798 3086 Size: 399 436 23 15 146 175 213 288 Cuts at: 3086 3620 4362 4447 5518 1391 Size: 534 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 534 436 399 288 213 175 146 85 23 15 BspEI T'CCGG_A Cuts at: 2044 2547 5964 2044 Size: 503 3417 2381 Fragments arranged by size: 3417 2381 503 BspHI T'CATG_A Cuts at: 4190 5920 4190 Size: 1730 4571 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2334 2354 2826 2993 3432 3525 3656 844 Size: 1490 20 472 167 439 93 131 3489 Fragments arranged by size: 3489 1490 472 439 167 131 93 20 BsrBI CCG'CTC Cuts at: 1394 1823 2470 4188 1394 Size: 429 647 1718 3507 Fragments arranged by size: 3507 1718 647 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3577 4796 4970 65 Size: 1183 2329 1219 174 1396 Fragments arranged by size: 2329 1396 1219 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2153 4945 5132 1700 Size: 110 343 2792 187 2869 Fragments arranged by size: 2869 2792 343 187 110 BsrGI T'GTAC_A Cuts at: 96 2322 3030 96 Size: 2226 708 3367 Fragments arranged by size: 3367 2226 708 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6301 BssSI C'ACGA_G Cuts at: 2486 3259 3527 4361 5373 2486 Size: 773 268 834 1012 3414 Fragments arranged by size: 3414 1012 834 773 268 BstAPI GCAn_nnn'nTGC Cuts at: 794 2366 794 Size: 1572 4729 BstBI TT'CG_AA Cuts at: 2929 5105 2929 Size: 2176 4125 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6301 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6301 BstYI r'GATC_y Cuts at: 1972 2014 2697 2806 2952 3631 3998 4383 4400 Size: 42 683 109 146 679 367 385 17 Cuts at: 4400 5852 5938 5950 6296 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 683 679 385 367 346 146 109 86 42 17 12 Bsu36I CC'TnA_GG Cuts at: 2112 2112 Size: 6301 BtgI C'CryG_G Cuts at: 513 1065 2004 2493 2520 3126 3301 3550 513 Size: 552 939 489 27 606 175 249 3264 Fragments arranged by size: 3264 939 606 552 489 249 175 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2307 487 Size: 683 56 1081 4481 Fragments arranged by size: 4481 1081 683 56 BtsI GCAGTG_nn' Cuts at: 2968 3817 4611 4631 2968 Size: 849 794 20 4638 Fragments arranged by size: 4638 849 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6301 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6266 DraI TTT'AAA Cuts at: 3605 3605 Size: 6301 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5308 809 Size: 507 3992 1802 Fragments arranged by size: 3992 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2935 3123 3139 Size: 54 1425 50 275 18 1105 188 16 Cuts at: 3139 3496 3748 4637 6266 8 Size: 357 252 889 1629 43 Fragments arranged by size: 1629 1425 1105 889 357 275 252 188 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 3748 1537 Size: 275 1936 4090 Fragments arranged by size: 4090 1936 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3014 4231 1353 Size: 1661 1217 3423 Fragments arranged by size: 3423 1661 1217 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2541 2993 4888 5262 5408 387 Size: 1064 138 952 452 1895 374 146 1280 Fragments arranged by size: 1895 1280 1064 952 452 374 146 138 EcoICRI GAG'CTC Cuts at: 2224 2408 2583 2224 Size: 184 175 5942 Fragments arranged by size: 5942 184 175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2251 2486 2602 3212 3229 3466 4364 Size: 393 619 235 116 610 17 237 898 Cuts at: 4364 4961 5748 6016 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 898 787 619 610 597 393 268 237 235 116 17 EcoNI CCTnn'n_nnAGG Cuts at: 3194 3194 Size: 6301 EcoO109I rG'GnC_Cy Cuts at: 2070 2127 2260 2261 2337 2070 Size: 57 133 1 76 6034 Fragments arranged by size: 6034 133 76 57 1 EcoRI G'AATT_C Cuts at: 3610 3988 3610 Size: 378 5923 FspI TGC'GCA Cuts at: 4807 4807 Size: 6301 HaeII r_GCGC'y Cuts at: 1678 5448 1678 Size: 3770 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2474 2506 2760 2792 4942 Size: 32 135 32 902 32 254 32 2150 Cuts at: 4942 4974 5016 5048 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2150 902 254 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3645 3891 669 Size: 988 281 1707 246 3079 Fragments arranged by size: 3079 1707 988 281 246 HindIII A'AGCT_T Cuts at: 748 2735 2924 748 Size: 1987 189 4125 Fragments arranged by size: 4125 1987 189 HpaI GTT'AAC Cuts at: 3891 3891 Size: 6301 KpnI G_GTAC'C Cuts at: 3626 3626 Size: 6301 MfeI C'AATT_G Cuts at: 3900 3900 Size: 6301 MluI A'CGCG_T Cuts at: 6177 6177 Size: 6301 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2871 5415 5599 1305 Size: 1566 2544 184 2007 Fragments arranged by size: 2544 2007 1566 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2937 3125 3141 3498 10 Size: 54 1425 343 1105 188 16 357 2813 Fragments arranged by size: 2813 1425 1105 357 343 188 54 16 MslI CAynn'nnrTG Cuts at: 518 1166 2385 3590 4259 4618 4777 518 Size: 648 1219 1205 669 359 159 2042 Fragments arranged by size: 2042 1219 1205 669 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2622 2716 3028 3331 3388 3544 3552 3717 Size: 1016 94 312 303 57 156 8 165 Cuts at: 3717 4394 5542 5787 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 1016 677 312 303 245 165 156 94 57 8 NcoI C'CATG_G Cuts at: 513 1065 2004 3126 513 Size: 552 939 1122 3688 Fragments arranged by size: 3688 1122 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6301 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6301 NotI GC'GGCC_GC Cuts at: 3748 3748 Size: 6301 NspI r_CATG'y Cuts at: 1132 1229 3659 5204 1132 Size: 97 2430 1545 2229 Fragments arranged by size: 2430 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2066 3397 1348 Size: 718 1331 4252 Fragments arranged by size: 4252 1331 718 PciI A'CATG_T Cuts at: 5200 5200 Size: 6301 PflMI CCAn_nnn'nTGG Cuts at: 1108 2066 1108 Size: 958 5343 PfoI T'CCnGG_A Cuts at: 3375 3375 Size: 6301 PmeI GTTT'AAAC Cuts at: 3605 3605 Size: 6301 PmlI CAC'GTG Cuts at: 2383 2383 Size: 6301 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4370 4402 5883 5915 4370 Size: 32 1481 32 4756 Fragments arranged by size: 4756 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2070 2127 2070 Size: 57 6244 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6301 PsiI TTA'TAA Cuts at: 3871 3871 Size: 6301 PspOMI G'GGCC_C Cuts at: 2260 2260 Size: 6301 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2558 2590 2558 Size: 32 6269 PstI C_TGCA'G Cuts at: 830 2231 2345 2715 2823 2963 3429 3525 3653 Size: 1401 114 370 108 140 466 96 128 Cuts at: 3653 830 Size: 3478 Fragments arranged by size: 3478 1401 466 370 140 128 114 108 96 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6301 PvuII CAG'CTG Cuts at: 2622 2716 3028 3544 2622 Size: 94 312 516 5379 Fragments arranged by size: 5379 516 312 94 SacI G_AGCT'C Cuts at: 2226 2410 2585 2226 Size: 184 175 5942 Fragments arranged by size: 5942 184 175 SacII CC_GC'GG Cuts at: 3553 3553 Size: 6301 SalI G'TCGA_C Cuts at: 1936 3643 1936 Size: 1707 4594 SanDI GG'GwC_CC Cuts at: 2070 2070 Size: 6301 SbfI CC_TGCA'GG Cuts at: 2345 3653 2345 Size: 1308 4993 ScaI AGT'ACT Cuts at: 4549 6261 4549 Size: 1712 4589 SfcI C'TryA_G Cuts at: 826 1045 2227 2341 2711 2819 2959 3425 3521 Size: 219 1182 114 370 108 140 466 96 Cuts at: 3521 3649 4784 5465 5656 826 Size: 128 1135 681 191 1471 Fragments arranged by size: 1471 1182 1135 681 466 370 219 191 140 128 114 108 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6301 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6301 SmaI CCC'GGG Cuts at: 1872 3170 3628 1872 Size: 1298 458 4545 Fragments arranged by size: 4545 1298 458 SmlI C'TyrA_G Cuts at: 820 1017 1943 2416 3094 4405 5306 5568 820 Size: 197 926 473 678 1311 901 262 1553 Fragments arranged by size: 1553 1311 926 901 678 473 262 197 SnaBI TAC'GTA Cuts at: 493 4092 493 Size: 3599 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6301 SphI G_CATG'C Cuts at: 1132 3659 1132 Size: 2527 3774 SspI AAT'ATT Cuts at: 5 52 4225 5 Size: 47 4173 2081 Fragments arranged by size: 4173 2081 47 StuI AGG'CCT Cuts at: 1517 3206 1517 Size: 1689 4612 StyI C'CwwG_G Cuts at: 513 1065 2004 2790 3126 3740 513 Size: 552 939 786 336 614 3074 Fragments arranged by size: 3074 939 786 614 552 336 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3302 4328 4345 4498 4683 5128 325 Size: 1255 1722 1026 17 153 185 445 1498 Fragments arranged by size: 1722 1498 1255 1026 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2322 3030 3477 Size: 274 80 33 51 1443 345 708 447 Cuts at: 3477 4547 6259 96 Size: 1070 1712 138 Fragments arranged by size: 1712 1443 1070 708 447 345 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6261 6293 6261 Size: 32 6269 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6301 XbaI T'CTAG_A Cuts at: 3637 3637 Size: 6301 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2854 3335 2854 Size: 481 5820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6301 XmaI C'CCGG_G Cuts at: 1870 3168 3626 1870 Size: 1298 458 4545 Fragments arranged by size: 4545 1298 458 XmnI GAAnn'nnTTC Cuts at: 2555 4430 2555 Size: 1875 4426 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4109 276 Size: 53 83 186 936 2575 2468 Fragments arranged by size: 2575 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI RsrII SapI SexAI SfiI SfoI SrfI SwaI