(Linear) (Six-base) MAPSORT of: KIEE6720.seq Check: 7671 from: 1 to: 2259 KIEE6720.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. AccI GT'mk_AC Cuts at: 0 497 1352 2259 Size: 497 855 907 Fragments arranged by size: 907 855 497 Acc65I G'GTAC_C Cuts at: 0 1210 1653 2065 2259 Size: 1210 443 412 194 Fragments arranged by size: 1210 443 412 194 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1375 1952 2259 Size: 1375 577 307 Fragments arranged by size: 1375 577 307 AflIII A'CryG_T Cuts at: 0 283 2259 Size: 283 1976 AleI CACnn'nnGTG Cuts at: 0 1543 2259 Size: 1543 716 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 314 2152 2259 Size: 314 1838 107 Fragments arranged by size: 1838 314 107 ApoI r'AATT_y Cuts at: 0 660 2259 Size: 660 1599 AvaI C'yCGr_G Cuts at: 0 155 2259 Size: 155 2104 BanI G'GyrC_C Cuts at: 0 139 871 1210 1653 1948 2065 2259 Size: 139 732 339 443 295 117 194 Fragments arranged by size: 732 443 339 295 194 139 117 BbeI G_GCGC'C Cuts at: 0 143 2259 Size: 143 2116 BbsI GAAGACnn'nnnn_ Cuts at: 0 1090 2259 Size: 1090 1169 BfrBI ATG'CAT Cuts at: 0 18 2259 Size: 18 2241 BglI GCCn_nnn'nGGC Cuts at: 0 43 2259 Size: 43 2216 BglII A'GATC_T Cuts at: 0 405 1227 2259 Size: 405 822 1032 Fragments arranged by size: 1032 822 405 Bme1580I G_kGCm'C Cuts at: 0 876 2259 Size: 876 1383 BmrI ACTGGGnnnn_n' Cuts at: 0 870 1879 2259 Size: 870 1009 380 Fragments arranged by size: 1009 870 380 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 328 2259 Size: 328 1931 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 626 2259 Size: 238 388 1633 Fragments arranged by size: 1633 388 238 Bpu10I CC'TnA_GC Cuts at: 0 108 727 2259 Size: 108 619 1532 Fragments arranged by size: 1532 619 108 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1330 1807 2259 Size: 1330 477 452 Fragments arranged by size: 1330 477 452 BsaBI GATnn'nnATC Cuts at: 0 79 2259 Size: 79 2180 BsaHI Gr'CG_yC Cuts at: 0 140 2259 Size: 140 2119 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1217 1247 2259 Size: 1217 30 1012 Fragments arranged by size: 1217 1012 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 320 1850 2206 2259 Size: 320 1530 356 53 Fragments arranged by size: 1530 356 320 53 BseYI C'CCAG_C Cuts at: 0 29 432 2259 Size: 29 403 1827 Fragments arranged by size: 1827 403 29 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2158 2259 Size: 2158 101 BsiEI CG_ry'CG Cuts at: 0 38 2259 Size: 38 2221 BsiHKAI G_wGCw'C Cuts at: 0 1187 2259 Size: 1187 1072 BsmI GAATG_Cn' Cuts at: 0 18 919 1787 2259 Size: 18 901 868 472 Fragments arranged by size: 901 868 472 18 Bsp1286I G_dGCh'C Cuts at: 0 876 1187 2259 Size: 876 311 1072 Fragments arranged by size: 1072 876 311 BspHI T'CATG_A Cuts at: 0 1764 2259 Size: 1764 495 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 979 1198 2259 Size: 979 219 1061 Fragments arranged by size: 1061 979 219 BsrBI CCG'CTC Cuts at: 0 154 2259 Size: 154 2105 BsrDI GCAATG_nn' Cuts at: 0 377 1988 2259 Size: 377 1611 271 Fragments arranged by size: 1611 377 271 BsrFI r'CCGG_y Cuts at: 0 62 2259 Size: 62 2197 BstAPI GCAn_nnn'nTGC Cuts at: 0 994 2259 Size: 994 1265 BstBI TT'CG_AA Cuts at: 0 53 630 1779 2259 Size: 53 577 1149 480 Fragments arranged by size: 1149 577 480 53 BstEII G'GTnAC_C Cuts at: 0 888 2259 Size: 888 1371 BstXI CCAn_nnnn'nTGG Cuts at: 0 432 2259 Size: 432 1827 BstYI r'GATC_y Cuts at: 0 405 711 1227 2259 Size: 405 306 516 1032 Fragments arranged by size: 1032 516 405 306 Bsu36I CC'TnA_GG Cuts at: 0 1658 1727 2259 Size: 1658 69 532 Fragments arranged by size: 1658 532 69 BtgI C'CryG_G Cuts at: 0 38 563 1415 2073 2259 Size: 38 525 852 658 186 Fragments arranged by size: 852 658 525 186 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 28 2259 Size: 28 2231 BtsI GCAGTG_nn' Cuts at: 0 124 1009 1649 1748 2259 Size: 124 885 640 99 511 Fragments arranged by size: 885 640 511 124 99 DraI TTT'AAA Cuts at: 0 486 2259 Size: 486 1773 DraIII CAC_nnn'GTG Cuts at: 0 941 2259 Size: 941 1318 DrdI GACnn_nn'nnGTC Cuts at: 0 1885 2259 Size: 1885 374 EaeI y'GGCC_r Cuts at: 0 35 262 1505 2259 Size: 35 227 1243 754 Fragments arranged by size: 1243 754 227 35 EagI C'GGCC_G Cuts at: 0 35 2259 Size: 35 2224 EarI CTCTTCn'nnn_ Cuts at: 0 1678 1928 2259 Size: 1678 250 331 Fragments arranged by size: 1678 331 250 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 238 626 1375 1952 2259 Size: 238 388 749 577 307 Fragments arranged by size: 749 577 388 307 238 EcoO109I rG'GnC_Cy Cuts at: 0 1878 2259 Size: 1878 381 EcoRI G'AATT_C Cuts at: 0 660 2259 Size: 660 1599 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 391 856 2259 Size: 391 465 1403 Fragments arranged by size: 1403 465 391 HaeII r_GCGC'y Cuts at: 0 7 30 63 143 2259 Size: 7 23 33 80 2116 Fragments arranged by size: 2116 80 33 23 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 114 146 1298 1330 1368 1400 2259 Size: 114 32 1152 32 38 32 859 Fragments arranged by size: 1152 859 114 38 32 32 32 HincII GTy'rAC Cuts at: 0 2006 2259 Size: 2006 253 HindIII A'AGCT_T Cuts at: 0 1825 2259 Size: 1825 434 KasI G'GCGC_C Cuts at: 0 139 2259 Size: 139 2120 KpnI G_GTAC'C Cuts at: 0 1214 1657 2069 2259 Size: 1214 443 412 190 Fragments arranged by size: 1214 443 412 190 MfeI C'AATT_G Cuts at: 0 1856 2259 Size: 1856 403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1203 2259 Size: 1203 1056 MscI TGG'CCA Cuts at: 0 264 1507 2259 Size: 264 1243 752 Fragments arranged by size: 1243 752 264 MslI CAynn'nnrTG Cuts at: 0 296 1321 1543 1793 2259 Size: 296 1025 222 250 466 Fragments arranged by size: 1025 466 296 250 222 MspA1I CmG'CkG Cuts at: 0 40 2259 Size: 40 2219 NaeI GCC'GGC Cuts at: 0 64 2259 Size: 64 2195 NarI GG'CG_CC Cuts at: 0 140 2259 Size: 140 2119 NcoI C'CATG_G Cuts at: 0 563 2073 2259 Size: 563 1510 186 Fragments arranged by size: 1510 563 186 NgoMIV G'CCGG_C Cuts at: 0 62 2259 Size: 62 2197 NotI GC'GGCC_GC Cuts at: 0 35 2259 Size: 35 2224 NruI TCG'CGA Cuts at: 0 13 2259 Size: 13 2246 NsiI A_TGCA'T Cuts at: 0 20 2259 Size: 20 2239 NspI r_CATG'y Cuts at: 0 287 528 2259 Size: 287 241 1731 Fragments arranged by size: 1731 287 241 PciI A'CATG_T Cuts at: 0 283 2259 Size: 283 1976 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1805 1837 2259 Size: 1805 32 422 Fragments arranged by size: 1805 422 32 PpuMI rG'GwC_Cy Cuts at: 0 1878 2259 Size: 1878 381 PspXI vC'TCGA_Gb Cuts at: 0 155 2259 Size: 155 2104 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 891 923 2259 Size: 891 32 1336 Fragments arranged by size: 1336 891 32 PstI C_TGCA'G Cuts at: 0 1494 2259 Size: 1494 765 SacII CC_GC'GG Cuts at: 0 41 2259 Size: 41 2218 SbfI CC_TGCA'GG Cuts at: 0 1494 2259 Size: 1494 765 SfcI C'TryA_G Cuts at: 0 494 1001 1490 2259 Size: 494 507 489 769 Fragments arranged by size: 769 507 494 489 SfoI GGC'GCC Cuts at: 0 141 2259 Size: 141 2118 SmlI C'TyrA_G Cuts at: 0 155 1345 1822 2259 Size: 155 1190 477 437 Fragments arranged by size: 1190 477 437 155 SspI AAT'ATT Cuts at: 0 465 2259 Size: 465 1794 StyI C'CwwG_G Cuts at: 0 143 563 894 2073 2259 Size: 143 420 331 1179 186 Fragments arranged by size: 1179 420 331 186 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 433 1372 1816 2259 Size: 433 939 444 443 Fragments arranged by size: 939 444 443 433 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1487 1519 2259 Size: 1487 32 740 Fragments arranged by size: 1487 740 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 465 1959 2259 Size: 465 1494 300 Fragments arranged by size: 1494 465 300 XhoI C'TCGA_G Cuts at: 0 155 2259 Size: 155 2104 XmnI GAAnn'nnTTC Cuts at: 0 1631 2250 2259 Size: 1631 619 9 Fragments arranged by size: 1631 619 9 Enzymes that do cut: AccI Acc65I AcuI AflIII AleI AlfI ApoI AvaI BanI BbeI BbsI BfrBI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII KasI KpnI MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI PciI PpiI PpuMI PspXI PsrI PstI SacII SbfI SfcI SfoI SmlI SspI StyI TaqII TstI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BanII BbvCI BcgI BciVI BclI BlpI BmgBI BmtI BsaI BsaAI BsaWI BsiWI BsmBI BspEI BsrGI BssHII BssSI BstZ17I ClaI CspCI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI HpaI MluI NdeI NheI PacI PasI PflMI PfoI PmeI PmlI PshAI PsiI PspOMI PvuI PvuII RsrII SacI SalI SanDI SapI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI Tth111I XbaI XmaI ZraI