Restriction map for
pFN21AB6611
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AviII AvrII BamHI
BbrPI BbvCI BcgI BclI BfaI
BfrI BlnI BlpI Bpu1102I BsaBI
BsaI BseAI BsiWI BsmAI BsmBI
BspDI BspEI BspHI BspLU11I BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoT14I EcoT22I FbaI FseI
FspI HpaI KpnI MaeI MamI
MfeI MluI MroI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI StuI
StyI SwaI Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcsI 9 AAATTT [GA]^AATT[CT]
AflIII 917 ACGTGT A^C[GA][CT]GT
AgeI 305 ACCGGT A^CCGGT
AlwI 159 GATCC GGATC(4/5)
AlwNI 58 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 9 AAATTT [GA]^AATT[CT]
Asp700I 798 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1209 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 585 CCCGGG C^[CT]CG[GA]G
BbeI 880 GGCGCC GGCGC^C
BglI 677 GCCGCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 863 AGATCT A^GATCT
BsaWI 305 ACCGGT [AT]^CCGG[AT]
BsiEI 870 CGACCG CG[GA][CT]^CG
BsmI 1195 GAATGC GAATGC(1/-1)
BsoBI 585 CCCGGG C^[CT]CG[GA]G
BsrFI 305 ACCGGT [GA]^CCGG[CT]
BsrI 571 ACTGG ACTGG(1/-1)
BstXI 344 CCAACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 305 ACCGGT [GA]^CCGG[CT]
DraI 12 TTTAAA TTT^AAA
DraII 59 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 124 GACTTTGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1231 CTCTTC CTCTTC(1/4)
EcoNI 519 CCTCAACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 59 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRV 197 GATATC GAT^ATC
HincII 964 GTCAAC GT[CT]^[GA]AC
HindII 964 GTCAAC GT[CT]^[GA]AC
HindIII 336 AAGCTT A^AGCTT
KasI 880 GGCGCC G^GCGCC
Ksp632I 1231 CTCTTC CTCTTC(1/4)
KspI 469 CCGCGG CCGC^GG
MseI 13 TTAA T^TAA
NarI 880 GGCGCC GG^CGCC
NspI 1097 ACATGC [GA]CATG^[CT]
PflFI 1209 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 305 ACCGGT A^CCGGT
PpuMI 59 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 52 CAGCTG CAG^CTG
SacII 469 CCGCGG CCGC^GG
SbfI 987 CCTGCAGG CCTGCA^GG
SfoI 880 GGCGCC GGC^GCC
SmaI 585 CCCGGG CCC^GGG
Sse8387I 987 CCTGCAGG CCTGCA^GG
SspI 173 AATATT AAT^ATT
Tru9I 13 TTAA T^TAA
Tsp509I 10 AATT ^AATT
Tth111I 1209 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1142 CCAACTTTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 585 CCCGGG C^CCGGG
XmaI 585 CCCGGG C^CCGGG
XmnI 798 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 163 CGCG CG^CG
470 CGCG
BalI 74 TGGCCA TGG^CCA
341 TGGCCA
BbsI 39 GTCTTC GAAGAC(2/6)
1003 GTCTTC
BciVI 251 GTATCC GTATCC(6/5)
719 GTATCC
BpmI 572 CTGGAG CTGGAG(16/14)
781 CTGGAG
BpuAI 39 GTCTTC GAAGAC(2/6)
1003 GTCTTC
BsaAI 817 TACGTG [CT]AC^GT[GA]
907 TACGTG
BseRI 841 GAGGAG GAGGAG(10/8)
1075 GAGGAG
BsgI 598 CTGCAC GTGCAG(16/14)
355 GTGCAG
BsmFI 426 GGGAC GGGAC(10/14)
1207 GGGAC
BspMI 986 ACCTGC ACCTGC(4/8)
990 GCAGGT
BsrBI 850 GAGCGG CCGCTC(-3/-3)
1024 GAGCGG
BstUI 163 CGCG CG^CG
470 CGCG
BstYI 158 AGATCC [GA]^GATC[CT]
863 AGATCT
EaeI 74 TGGCCA [CT]^GGCC[GA]
341 TGGCCA
HaeII 378 GGCGCT [GA]GCGC^[CT]
880 GGCGCC
MaeIII 594 GTCAC ^GT[ACGT]AC
977 GTGAC
MflI 158 AGATCC [GA]^GATC[CT]
863 AGATCT
MluNI 74 TGGCCA TGG^CCA
341 TGGCCA
MscI 74 TGGCCA TGG^CCA
341 TGGCCA
MvnI 163 CGCG CG^CG
470 CGCG
SacI 400 GAGCTC GAGCT^C
1078 GAGCTC
SmlI 238 CTCAAG C^T[CT][GA]AG
256 CTCAAG
TaqI 134 TCGA T^CGA
639 TCGA
Tsp45I 594 GTCAC ^GT[GC]AC
977 GTGAC
TthHB8I 134 TCGA T^CGA
639 TCGA
XhoII 158 AGATCC [GA]^GATC[CT]
863 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaII BanI BanII BbvI BcnI
BmyI BsaHI BsaJI BsiHKAI BsiYI
BslI Bsp1286I Bsp1407I BsrGI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EcoRII FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SexAI SfaNI SfcI SspBI
TfiI TseI TspRI