(Circular) (Six-base) MAPSORT of: pFN21AB6611.seq Check: 8519 from: 1 to: 5941 pFN21AB6611 5941 bp With 182 enzymes: SgfI * October 20, 2009 10:57 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2986 2986 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3284 1937 Size: 1347 4594 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4004 5388 5656 1632 Size: 2372 1384 268 1917 Fragments arranged by size: 2372 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2922 4840 5817 1203 Size: 1719 1918 977 1327 Fragments arranged by size: 1918 1719 1327 977 AgeI A'CCGG_T Cuts at: 2310 4772 2310 Size: 2462 3479 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 2979 1166 Size: 1813 4128 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2628 1184 Size: 1444 4497 AlwNI CAG_nnn'CTG Cuts at: 2068 3684 5256 2068 Size: 1616 1572 2753 Fragments arranged by size: 2753 1616 1572 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 2014 3250 3476 3628 5737 1493 Size: 521 1236 226 152 2109 1697 Fragments arranged by size: 2109 1697 1236 521 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2590 3266 1870 Size: 73 647 676 4545 Fragments arranged by size: 4545 676 647 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2304 2328 2569 2885 3262 Size: 325 217 627 517 24 241 316 377 Cuts at: 3262 4717 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 517 377 325 316 241 217 24 BanII G_rGCy'C Cuts at: 1391 2409 3087 3168 3260 1391 Size: 1018 678 81 92 4072 Fragments arranged by size: 4072 1018 678 92 81 BbeI G_GCGC'C Cuts at: 2889 2889 Size: 5941 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 3001 928 Size: 971 138 964 3868 Fragments arranged by size: 3868 971 964 138 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2267 2735 3833 5043 2267 Size: 468 1098 1210 3165 Fragments arranged by size: 3165 1210 1098 468 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2688 4552 136 Size: 107 122 71 631 605 1016 1864 1525 Fragments arranged by size: 1864 1525 1016 631 605 122 107 71 BglII A'GATC_T Cuts at: 2868 5936 2868 Size: 3068 2873 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2572 4002 5158 1790 Size: 782 1430 1156 2573 Fragments arranged by size: 2573 1430 1156 782 BmgBI CAC'GTC Cuts at: 1318 2923 1318 Size: 1605 4336 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2689 2689 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2598 2807 4601 1239 Size: 1359 209 1794 2579 Fragments arranged by size: 2579 1794 1359 209 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 2246 4066 4931 5229 2228 Size: 18 1820 865 298 2940 Fragments arranged by size: 2940 1820 865 298 18 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2824 2914 3732 493 Size: 711 1620 90 818 2702 Fragments arranged by size: 2702 1620 818 711 90 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2597 2775 2886 3748 Size: 53 83 186 936 1064 178 111 862 Cuts at: 3748 4130 4765 275 Size: 382 635 1451 Fragments arranged by size: 1451 1064 936 862 635 382 186 178 111 83 53 BsaWI w'CCGG_w Cuts at: 2310 4374 4772 5046 5193 5604 2310 Size: 2064 398 274 147 411 2647 Fragments arranged by size: 2647 2064 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2909 2939 1178 Size: 30 165 30 1506 30 4180 Fragments arranged by size: 4180 1506 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2861 3095 1186 Size: 1675 234 4032 Fragments arranged by size: 4032 1675 234 BseYI C'CCAG_C Cuts at: 2394 2769 3161 5144 5640 2394 Size: 375 392 1983 496 2695 Fragments arranged by size: 2695 1983 496 392 375 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2381 2588 1163 Size: 573 645 207 4516 Fragments arranged by size: 4516 645 573 207 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2878 3391 4152 5180 1540 Size: 275 186 877 513 761 1028 2301 Fragments arranged by size: 2301 1028 877 761 513 275 186 BsiHKAI G_wGCw'C Cuts at: 2222 2409 3087 3134 4002 4087 5158 2222 Size: 187 678 47 868 85 1071 3005 Fragments arranged by size: 3005 1071 868 678 187 85 47 BsmI GAATG_Cn' Cuts at: 3206 3452 3545 3206 Size: 246 93 5602 Fragments arranged by size: 5602 246 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2222 2409 2572 3087 3134 3168 3260 Size: 399 432 187 163 515 47 34 92 Cuts at: 3260 4002 4087 5158 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 432 399 187 163 92 85 47 34 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3830 5560 3830 Size: 1730 4211 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2986 3000 3296 844 Size: 2142 14 296 3489 Fragments arranged by size: 3489 2142 296 14 BsrBI CCG'CTC Cuts at: 1394 1823 2857 3031 3828 1394 Size: 429 1034 174 797 3507 Fragments arranged by size: 3507 1034 797 429 174 BsrDI GCAATG_nn' Cuts at: 65 1248 4436 4610 65 Size: 1183 3188 174 1396 Fragments arranged by size: 3188 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2310 4585 4772 1700 Size: 110 500 2275 187 2869 Fragments arranged by size: 2869 2275 500 187 110 BsrGI T'GTAC_A Cuts at: 96 2487 2925 3000 96 Size: 2391 438 75 3037 Fragments arranged by size: 3037 2391 438 75 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2356 2356 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2163 2868 3271 3638 4023 4040 5492 5578 Size: 191 705 403 367 385 17 1452 86 Cuts at: 5578 5590 5936 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 705 403 385 367 346 191 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2231 2474 2931 3117 513 Size: 552 939 227 243 457 186 3337 Fragments arranged by size: 3337 939 552 457 243 227 186 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3028 487 Size: 683 56 1802 3400 Fragments arranged by size: 3400 1802 683 56 BtsI GCAGTG_nn' Cuts at: 2600 3457 4251 4271 2600 Size: 857 794 20 4270 Fragments arranged by size: 4270 857 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2691 2726 529 Size: 35 2127 35 3744 Fragments arranged by size: 3744 2127 35 35 DraI TTT'AAA Cuts at: 2019 3245 2019 Size: 1226 4715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2135 4948 809 Size: 507 819 2813 1802 Fragments arranged by size: 2813 1802 819 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2079 2346 3388 Size: 54 1425 50 275 18 249 267 1042 Cuts at: 3388 4277 5906 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1042 889 275 267 249 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3242 3871 1353 Size: 1889 629 3423 Fragments arranged by size: 3423 1889 629 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4528 4902 5048 387 Size: 1064 138 435 2504 374 146 1280 Fragments arranged by size: 2504 1280 1064 435 374 146 138 EcoICRI GAG'CTC Cuts at: 2407 3085 2407 Size: 678 5263 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2598 2807 4004 4601 5388 5656 1239 Size: 393 966 209 1197 597 787 268 1524 Fragments arranged by size: 1524 1197 966 787 597 393 268 209 EcoNI CCTnn'n_nnAGG Cuts at: 2528 2528 Size: 5941 EcoO109I rG'GnC_Cy Cuts at: 2065 2065 Size: 5941 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 EcoRV GAT'ATC Cuts at: 2204 2204 Size: 5941 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2824 2824 Size: 5941 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2387 2889 5088 1678 Size: 709 502 2199 2531 Fragments arranged by size: 2531 2199 709 502 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2721 2753 4582 4614 4656 Size: 32 135 32 1149 32 1829 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1829 1149 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2971 3285 3531 669 Size: 988 281 1033 314 246 3079 Fragments arranged by size: 3079 1033 988 314 281 246 HindIII A'AGCT_T Cuts at: 748 2341 748 Size: 1593 4348 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2885 2885 Size: 5941 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3171 5055 5239 1305 Size: 1866 1884 184 2007 Fragments arranged by size: 2007 1884 1866 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2081 2348 10 Size: 54 1425 343 249 267 3603 Fragments arranged by size: 3603 1425 343 267 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2979 3107 3137 3899 4258 4417 518 Size: 648 1813 128 30 762 359 159 2042 Fragments arranged by size: 2042 1813 762 648 359 159 128 30 MspA1I CmG'CkG Cuts at: 1606 2059 2476 2679 2685 3357 4034 5182 5427 Size: 453 417 203 6 672 677 1148 245 Cuts at: 5427 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 672 453 417 245 203 6 NarI GG'CG_CC Cuts at: 2886 2886 Size: 5941 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 3106 3299 4844 1132 Size: 97 1877 193 1545 2229 Fragments arranged by size: 2229 1877 1545 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2065 2065 Size: 5941 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PstI C_TGCA'G Cuts at: 830 2030 2064 2997 3293 830 Size: 1200 34 933 296 3478 Fragments arranged by size: 3478 1200 933 296 34 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2059 2059 Size: 5941 SacI G_AGCT'C Cuts at: 2409 3087 2409 Size: 678 5263 SacII CC_GC'GG Cuts at: 2477 2477 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3283 1936 Size: 1347 4594 SbfI CC_TGCA'GG Cuts at: 2997 3293 2997 Size: 296 5645 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SexAI A'CCwGG_T Cuts at: 2184 2355 2529 2184 Size: 171 174 5596 Fragments arranged by size: 5596 174 171 SfcI C'TryA_G Cuts at: 826 1045 2026 2060 2442 2993 3289 4424 5105 Size: 219 981 34 382 551 296 1135 681 Cuts at: 5105 5296 826 Size: 191 1471 Fragments arranged by size: 1471 1135 981 681 551 382 296 219 191 34 SfoI GGC'GCC Cuts at: 2887 2887 Size: 5941 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2592 3268 1872 Size: 720 676 4545 Fragments arranged by size: 4545 720 676 SmlI C'TyrA_G Cuts at: 820 1017 1943 2243 2261 4045 4946 5208 820 Size: 197 926 300 18 1784 901 262 1553 Fragments arranged by size: 1784 1553 926 901 300 262 197 18 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 2180 3865 5 Size: 47 2128 1685 2081 Fragments arranged by size: 2128 2081 1685 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 3380 513 Size: 552 939 1376 3074 Fragments arranged by size: 3074 1376 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2587 3968 3985 4138 4323 4768 325 Size: 1255 1007 1381 17 153 185 445 1498 Fragments arranged by size: 1498 1381 1255 1007 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2487 2925 3000 Size: 274 80 33 51 1443 510 438 75 Cuts at: 3000 4187 5899 96 Size: 1187 1712 138 Fragments arranged by size: 1712 1443 1187 510 438 274 138 80 75 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 5901 5933 2549 Size: 32 3320 32 2557 Fragments arranged by size: 3320 2557 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3217 1316 Size: 1901 4040 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3154 3154 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2590 3266 1870 Size: 720 676 4545 Fragments arranged by size: 4545 720 676 XmnI GAAnn'nnTTC Cuts at: 2807 4070 2807 Size: 1263 4678 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SanDI SapI SfiI SrfI SwaI