################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstYI BstZ17I Bsu36I ClaI DraI DraII DraIII Eam1105I EarI Eco47III Eco81I EcoO109I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HindIII HinfI HpaI KpnI Ksp632I KspI MaeI MamI MboII MfeI MflI MluI MluNI MroI MscI MseI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AflIII 299 ACGTGT A^C[GA][CT]GT AlwI 201 GGATC GGATC(4/5) AspI 355 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 305 CCCGGG C^[CT]CG[GA]G AvaII 360 GGTCC G^G[AT]CC BcgI 380 GCAGCAAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 415 GCTCAGC GC^T[ACGT]AGC Bpu1102I 415 GCTCAGC GC^T[ACGT]AGC BsaI 547 GGTCTC GGTCTC(1/5) BsiEI 390 CGGCCG CG[GA][CT]^CG BsmAI 548 GTCTC GTCTC(1/5) BsmI 643 GAATGC GAATGC(1/-1) BsoBI 305 CCCGGG C^[CT]CG[GA]G BsrFI 392 GCCGGT [GA]^CCGG[CT] BstBI 640 TTCGAA TT^CGAA BstEII 30 GGTGACC G^GT[ACGT]ACC BstPI 30 GGTGACC G^GT[ACGT]ACC BstXI 473 CCATCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 415 GCTCAGC GC^T[ACGT]AGC Cfr10I 392 GCCGGT [GA]^CCGG[CT] CpoI 359 CGGTCCG CG^G[AT]CCG DrdI 9 GACGGGGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 390 CGGCCG C^GGCCG EclXI 390 CGGCCG C^GGCCG Eco52I 390 CGGCCG C^GGCCG EcoNI 444 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 30 GGTGACC G^GT[ACGT]ACC EcoT14I 282 CCTTGG C^C[AT][AT]GG HincII 175 GTTGAC GT[CT]^[GA]AC HindII 175 GTTGAC GT[CT]^[GA]AC MspA1I 489 CCGCTG C[AC]G^C[GT]G NspV 640 TTCGAA TT^CGAA PflFI 355 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 438 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsrII 359 CGGTCCG CG^G[AT]CCG SfuI 640 TTCGAA TT^CGAA SmaI 305 CCCGGG CCC^GGG StyI 282 CCTTGG C^C[AT][AT]GG TaqI 641 TCGA T^CGA Tth111I 355 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 641 TCGA T^CGA Van91I 438 CCAGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 648 CCAGGCCAAGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 305 CCCGGG C^CCGGG XmaI 305 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 274 GGCGCC G[GA]^CG[CT]C 661 GGCGCC AfaI 318 GTAC GT^AC 396 GTAC AlwNI 439 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG 486 CAGCCGCTG BanII 222 GGGCTC G[GA]GC[CT]^C 413 GGGCTC BbeI 274 GGCGCC GGCGC^C 661 GGCGCC BpmI 121 CTGGAG CTGGAG(16/14) 160 CTGGAG BsaHI 274 GGCGCC G[GA]^CG[CT]C 661 GGCGCC Cfr13I 360 GGTCC G^G[ACGT]CC 459 GGCCC DdeI 104 CTGAG C^T[ACGT]AG 416 CTCAG DpnII 202 GATC ^GATC 324 GATC DsaI 26 CCACGG C^C[GA][CT]GG 479 CCGTGG EaeI 390 CGGCCG [CT]^GGCC[GA] 446 TGGCCG Hin1I 274 GGCGCC G[GA]^CG[CT]C 661 GGCGCC KasI 274 GGCGCC G^GCGCC 661 GGCGCC MaeII 257 ACGT A^CGT 299 ACGT MboI 202 GATC ^GATC 324 GATC MslI 474 CATCCCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 531 CACCGTCATG NarI 274 GGCGCC GG^CGCC 661 GGCGCC NdeII 202 GATC ^GATC 324 GATC NlaIII 153 CATG CATG^ 537 CATG RsaI 318 GTAC GT^AC 396 GTAC Sau3AI 202 GATC ^GATC 324 GATC Sau96I 360 GGTCC G^G[ACGT]CC 459 GGCCC SfaNI 211 GATGC GCATC(5/9) 251 GCATC SfcI 189 CTACAG C^T[GA][CT]AG 233 CTGTAG SfoI 274 GGCGCC GGC^GCC 661 GGCGCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AciI AluI BanI BbvI BcnI BmyI BsaJI BseRI BsiYI BslI BsmFI Bsp1286I BsrBI BsrI BssKI BstF5I BstNI Cac8I CfoI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeIII MnlI MspI MvaI MwoI NciI NlaIV ScrFI TseI Tsp45I TspRI