(Linear) (Six-base) MAPSORT of: KIEE5208.seq Check: 2072 from: 1 to: 732 KIEE5208.orf With 182 enzymes: SgfI * January 29, 2014 11:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 394 732 Size: 394 338 AflIII A'CryG_T Cuts at: 0 299 732 Size: 299 433 AlwNI CAG_nnn'CTG Cuts at: 0 444 491 732 Size: 444 47 241 Fragments arranged by size: 444 241 47 AvaI C'yCGr_G Cuts at: 0 305 732 Size: 305 427 BanI G'GyrC_C Cuts at: 0 21 274 529 661 732 Size: 21 253 255 132 71 Fragments arranged by size: 255 253 132 71 21 BanII G_rGCy'C Cuts at: 0 226 417 732 Size: 226 191 315 Fragments arranged by size: 315 226 191 BbeI G_GCGC'C Cuts at: 0 278 665 732 Size: 278 387 67 Fragments arranged by size: 387 278 67 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 369 403 732 Size: 369 34 329 Fragments arranged by size: 369 329 34 BlpI GC'TnA_GC Cuts at: 0 416 732 Size: 416 316 Bme1580I G_kGCm'C Cuts at: 0 532 732 Size: 532 200 BmgBI CAC'GTC Cuts at: 0 258 300 732 Size: 258 42 432 Fragments arranged by size: 432 258 42 BmrI ACTGGGnnnn_n' Cuts at: 0 610 732 Size: 610 122 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 181 732 Size: 142 39 551 Fragments arranged by size: 551 142 39 BsaI GGTCTCn'nnnn_ Cuts at: 0 553 732 Size: 553 179 BsaHI Gr'CG_yC Cuts at: 0 275 662 732 Size: 275 387 70 Fragments arranged by size: 387 275 70 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 418 421 466 721 732 Size: 418 3 45 255 11 Fragments arranged by size: 418 255 45 11 3 BseYI C'CCAG_C Cuts at: 0 437 732 Size: 437 295 BsiEI CG_ry'CG Cuts at: 0 393 732 Size: 393 339 BsmI GAATG_Cn' Cuts at: 0 649 732 Size: 649 83 Bsp1286I G_dGCh'C Cuts at: 0 226 417 532 732 Size: 226 191 115 200 Fragments arranged by size: 226 200 191 115 BsrBI CCG'CTC Cuts at: 0 68 365 411 732 Size: 68 297 46 321 Fragments arranged by size: 321 297 68 46 BsrFI r'CCGG_y Cuts at: 0 392 732 Size: 392 340 BstBI TT'CG_AA Cuts at: 0 641 732 Size: 641 91 BstEII G'GTnAC_C Cuts at: 0 30 732 Size: 30 702 BstXI CCAn_nnnn'nTGG Cuts at: 0 480 732 Size: 480 252 BtgI C'CryG_G Cuts at: 0 26 479 732 Size: 26 453 253 Fragments arranged by size: 453 253 26 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 84 732 Size: 84 648 BtsI GCAGTG_nn' Cuts at: 0 624 732 Size: 624 108 DrdI GACnn_nn'nnGTC Cuts at: 0 15 732 Size: 15 717 EaeI y'GGCC_r Cuts at: 0 390 446 732 Size: 390 56 286 Fragments arranged by size: 390 286 56 EagI C'GGCC_G Cuts at: 0 390 732 Size: 390 342 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 142 181 394 732 Size: 142 39 213 338 Fragments arranged by size: 338 213 142 39 EcoNI CCTnn'n_nnAGG Cuts at: 0 448 732 Size: 448 284 HaeII r_GCGC'y Cuts at: 0 278 444 665 732 Size: 278 166 221 67 Fragments arranged by size: 278 221 166 67 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 286 318 732 Size: 286 32 414 Fragments arranged by size: 414 286 32 HincII GTy'rAC Cuts at: 0 177 732 Size: 177 555 KasI G'GCGC_C Cuts at: 0 274 661 732 Size: 274 387 71 Fragments arranged by size: 387 274 71 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 701 732 Size: 701 31 MslI CAynn'nnrTG Cuts at: 0 478 535 732 Size: 478 57 197 Fragments arranged by size: 478 197 57 MspA1I CmG'CkG Cuts at: 0 491 732 Size: 491 241 NarI GG'CG_CC Cuts at: 0 275 662 732 Size: 275 387 70 Fragments arranged by size: 387 275 70 PflMI CCAn_nnn'nTGG Cuts at: 0 444 732 Size: 444 288 PfoI T'CCnGG_A Cuts at: 0 701 732 Size: 701 31 RsrII CG'GwC_CG Cuts at: 0 360 732 Size: 360 372 SfcI C'TryA_G Cuts at: 0 189 233 732 Size: 189 44 499 Fragments arranged by size: 499 189 44 SfoI GGC'GCC Cuts at: 0 276 663 732 Size: 276 387 69 Fragments arranged by size: 387 276 69 SmaI CCC'GGG Cuts at: 0 307 732 Size: 307 425 StyI C'CwwG_G Cuts at: 0 282 732 Size: 282 450 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 39 732 Size: 39 693 TatI w'GTAC_w Cuts at: 0 317 732 Size: 317 415 Tth111I GACn'n_nGTC Cuts at: 0 358 732 Size: 358 374 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 655 732 Size: 655 77 XmaI C'CCGG_G Cuts at: 0 305 732 Size: 305 427 Enzymes that do cut: AcuI AflIII AlwNI AvaI BanI BanII BbeI BcgI BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaHI BseRI BseYI BsiEI BsmI Bsp1286I BsrBI BsrFI BstBI BstEII BstXI BtgI BtgZI BtsI DrdI EaeI EagI Eco57MI EcoNI HaeII Hin4I HincII KasI MmeI MslI MspA1I NarI PflMI PfoI RsrII SfcI SfoI SmaI StyI TaqII TatI Tth111I XcmI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BciVI BclI BfrBI BglI BglII BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EarI EciI EcoICRI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XhoI XmnI ZraI