Restriction map for
pFN21AB6142
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BbeI
BbvCI BfaI BfrI BglII BlnI
BsiWI BsmBI Bsp1407I BspDI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HpaI KasI KpnI MaeI
MfeI MflI MluI MunI NarI
NheI NruI NsiI NspV PacI
PaeR7I PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp45I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccII 889 CGCG CG^CG
AccIII 713 TCCGGA T^CCGGA
AgeI 81 ACCGGT A^CCGGT
AlwI 367 GATCC GGATC(4/5)
AspI 1270 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1832 TGGCCA TGG^CCA
BbrPI 1674 CACGTG CAC^GTG
BcgI 1292 GCAATGGGGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 463 GGATAC GTATCC(6/5)
BclI 395 TGATCA T^GATCA
BseAI 713 TCCGGA T^CCGGA
BsmI 1137 GCATTC GAATGC(1/-1)
BspEI 713 TCCGGA T^CCGGA
BspMI 1345 GCAGGT ACCTGC(4/8)
BsrDI 1292 GCAATG GCAATG(2/0)
BstUI 889 CGCG CG^CG
DraIII 1399 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 32 CGGCCG C^GGCCG
EclXI 32 CGGCCG C^GGCCG
Eco52I 32 CGGCCG C^GGCCG
EcoNI 219 CCTTGCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 395 TGATCA T^GATCA
HincII 1537 GTCAAC GT[CT]^[GA]AC
HindII 1537 GTCAAC GT[CT]^[GA]AC
HindIII 175 AAGCTT A^AGCTT
KspI 888 CCGCGG CCGC^GG
MluNI 1832 TGGCCA TGG^CCA
MroI 713 TCCGGA T^CCGGA
MscI 1832 TGGCCA TGG^CCA
MvnI 889 CGCG CG^CG
NaeI 1610 GCCGGC GCC^GGC
NdeI 769 CATATG CA^TATG
NgoMIV 1610 GCCGGC G^CCGGC
NotI 31 GCGGCCGC GC^GGCCGC
PflFI 1270 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 81 ACCGGT A^CCGGT
PmaCI 1674 CACGTG CAC^GTG
PmlI 1674 CACGTG CAC^GTG
SacI 847 GAGCTC GAGCT^C
SacII 888 CCGCGG CCGC^GG
SexAI 1802 ACCAGGT A^CC[AT]GGT
SmaI 842 CCCGGG CCC^GGG
StuI 752 AGGCCT AGG^CCT
Tth111I 1270 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 842 CCCGGG C^CCGGG
XmaI 842 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 1309 GAATTT [GA]^AATT[CT]
1494 AAATTT
AflIII 911 ACGTGT A^C[GA][CT]GT
1673 ACACGT
AlwNI 259 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
1228 CAGCATCTG
ApaI 807 GGGCCC GGGCC^C
838 GGGCCC
ApoI 1309 GAATTT [GA]^AATT[CT]
1494 AAATTT
AspHI 847 GAGCTC G[AT]GC[AT]^C
1240 GTGCTC
BlpI 73 GCTGAGC GC^T[ACGT]AGC
1605 GCTGAGC
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
1605 GCTGAGC
BsaAI 910 TACGTG [CT]AC^GT[GA]
1674 CACGTG
BsaBI 348 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
783 GATTGACATC
BsaI 272 GAGACC GGTCTC(1/5)
1556 GAGACC
BsaWI 81 ACCGGT [AT]^CCGG[AT]
713 TCCGGA
BsiEI 32 CGGCCG CG[GA][CT]^CG
79 CGACCG
BsiHKAI 847 GAGCTC G[AT]GC[AT]^C
1240 GTGCTC
BspHI 734 TCATGA T^CATGA
1661 TCATGA
CelII 73 GCTGAGC GC^T[ACGT]AGC
1605 GCTGAGC
HaeII 796 AGCGCC [GA]GCGC^[CT]
1729 AGCGCC
MaeII 911 ACGT A^CGT
1675 ACGT
MamI 348 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
783 GATTGACATC
NcoI 863 CCATGG C^CATGG
1352 CCATGG
PleI 1630 GACTC GAGTC(4/5)
167 GAGTC
PstI 856 CTGCAG CTGCA^G
1151 CTGCAG
RcaI 734 TCATGA T^CATGA
1661 TCATGA
SapI 1861 GAAGAGC GCTCTTC(1/4)
1040 GCTCTTC
TfiI 93 GAATC G^A[AT]TC
1394 GATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I Van91I XcmI