(Circular) (Six-base) MAPSORT of: pFN21AB6142.seq Check: 3778 from: 1 to: 6619 pFN21AB6142 6619 bp With 182 enzymes: SgfI * April 15, 2010 09:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3341 3341 Size: 6619 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4429 278 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3962 1937 Size: 2025 4594 Acc65I G'GTAC_C Cuts at: 3940 3940 Size: 6619 AclI AA'CG_TT Cuts at: 1563 3918 4746 5119 1563 Size: 2355 828 373 3063 Fragments arranged by size: 3063 2355 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2214 2472 2554 3052 3776 4682 6066 6334 Size: 582 258 82 498 724 906 1384 268 Cuts at: 6334 1632 Size: 1917 Fragments arranged by size: 1917 1384 906 724 582 498 268 258 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6422 AflIII A'CryG_T Cuts at: 1203 2916 3678 5518 6495 1203 Size: 1713 762 1840 977 1327 Fragments arranged by size: 1840 1713 1327 977 762 AgeI A'CCGG_T Cuts at: 2086 5450 2086 Size: 3364 3255 AhdI GACnn_n'nnGTC Cuts at: 5348 5348 Size: 6619 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6619 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2738 3476 1184 Size: 1554 738 4327 Fragments arranged by size: 4327 1554 738 AlwNI CAG_nnn'CTG Cuts at: 2269 3238 4362 5934 2269 Size: 969 1124 1572 2954 Fragments arranged by size: 2954 1572 1124 969 ApaI G_GGCC'C Cuts at: 2816 2847 2816 Size: 31 6588 ApaLI G'TGCA_C Cuts at: 4676 5832 4676 Size: 1156 5463 ApoI r'AATT_y Cuts at: 1493 3314 3499 3928 4154 4306 6415 1493 Size: 1821 185 429 226 152 2109 1697 Fragments arranged by size: 2109 1821 1697 429 226 185 152 AseI AT'TA_AT Cuts at: 160 1032 5173 160 Size: 872 4141 1606 Fragments arranged by size: 4141 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 AvaI C'yCGr_G Cuts at: 1870 1943 2847 3092 3167 3696 3944 1870 Size: 73 904 245 75 529 248 4545 Fragments arranged by size: 4545 904 529 248 245 75 73 BamHI G'GATC_C Cuts at: 3949 4316 3949 Size: 367 6252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3353 3593 3701 3940 5395 Size: 325 217 627 1566 240 108 239 1455 Cuts at: 5395 618 Size: 1842 Fragments arranged by size: 1842 1566 1455 627 325 240 239 217 108 BanII G_rGCy'C Cuts at: 1391 2816 2847 2856 3938 1391 Size: 1425 31 9 1082 4072 Fragments arranged by size: 4072 1425 1082 31 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2083 2248 3696 928 Size: 971 184 165 1448 3851 Fragments arranged by size: 3851 1448 971 184 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3286 3320 4810 4844 1903 Size: 34 1349 34 1490 34 3678 Fragments arranged by size: 3678 1490 1349 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2462 4511 5721 2462 Size: 2049 1210 3360 Fragments arranged by size: 3360 2049 1210 BclI T'GATC_A Cuts at: 1548 2400 1548 Size: 852 5767 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2898 3104 3170 Size: 107 122 71 631 605 1226 206 66 Cuts at: 3170 5230 136 Size: 2060 1525 Fragments arranged by size: 2060 1525 1226 631 605 206 122 107 71 66 BglII A'GATC_T Cuts at: 6614 6614 Size: 6619 BlpI GC'TnA_GC Cuts at: 2079 3611 4036 2079 Size: 1532 425 4662 Fragments arranged by size: 4662 1532 425 Bme1580I G_kGCm'C Cuts at: 1790 2816 2847 3596 3796 4680 5836 1790 Size: 1026 31 749 200 884 1156 2573 Fragments arranged by size: 2573 1156 1026 884 749 200 31 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6619 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2020 2418 4350 Size: 416 53 83 399 24 603 398 1932 Cuts at: 4350 5308 442 Size: 958 1753 Fragments arranged by size: 1932 1753 958 603 416 399 398 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6619 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2779 3061 2779 Size: 282 6337 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2959 3218 3536 3734 3881 5279 1239 Size: 1052 668 259 318 198 147 1398 2579 Fragments arranged by size: 2579 1398 1052 668 318 259 198 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4744 5609 5907 4744 Size: 865 298 5456 Fragments arranged by size: 5456 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2271 3555 5282 882 Size: 617 772 1284 1727 2219 Fragments arranged by size: 2219 1727 1284 772 617 BsaAI yAC'GTr Cuts at: 493 1204 2917 3681 4410 493 Size: 711 1713 764 729 2702 Fragments arranged by size: 2702 1713 764 729 711 BsaBI GATnn'nnATC Cuts at: 2357 2792 6613 2357 Size: 435 3821 2363 Fragments arranged by size: 3821 2363 435 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3480 3619 3693 4426 Size: 53 83 186 936 1947 139 74 733 Cuts at: 4426 4808 5443 275 Size: 382 635 1451 Fragments arranged by size: 1947 1451 936 733 635 382 186 139 83 74 53 BsaWI w'CCGG_w Cuts at: 2086 2718 5052 5450 5724 5871 6282 2086 Size: 632 2334 398 274 147 411 2423 Fragments arranged by size: 2423 2334 632 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2522 2552 2673 2703 3405 Size: 30 165 30 1119 30 121 30 702 Cuts at: 3405 3435 1178 Size: 30 4362 Fragments arranged by size: 4362 1119 702 165 121 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2752 3573 3869 1186 Size: 1566 821 296 3936 Fragments arranged by size: 3936 1566 821 296 BseYI C'CCAG_C Cuts at: 2464 2808 3718 5822 6318 2464 Size: 344 910 2104 496 2765 Fragments arranged by size: 2765 2104 910 496 344 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2661 3115 3443 1163 Size: 573 925 454 328 4339 Fragments arranged by size: 4339 925 573 454 328 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2040 2087 4069 4830 5858 1540 Size: 275 186 39 47 1982 761 1028 2301 Fragments arranged by size: 2301 1982 1028 761 275 186 47 39 BsiHKAI G_wGCw'C Cuts at: 2856 3249 4680 4765 5836 2856 Size: 393 1431 85 1071 3639 Fragments arranged by size: 3639 1431 1071 393 85 BsmI GAATG_Cn' Cuts at: 3142 4130 4223 3142 Size: 988 93 5538 Fragments arranged by size: 5538 988 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2816 2847 2856 3249 3596 3796 3938 Size: 399 1026 31 9 393 347 200 142 Cuts at: 3938 4680 4765 5836 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1026 742 399 393 347 200 142 85 31 9 BspEI T'CCGG_A Cuts at: 2718 6282 2718 Size: 3564 3055 BspHI T'CATG_A Cuts at: 2739 3666 4508 6238 2739 Size: 927 842 1730 3120 Fragments arranged by size: 3120 1730 927 842 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3341 3974 844 Size: 2497 633 3489 Fragments arranged by size: 3489 2497 633 BsrBI CCG'CTC Cuts at: 1394 1823 4506 1394 Size: 429 2683 3507 Fragments arranged by size: 3507 2683 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3304 5114 5288 65 Size: 1183 2056 1810 174 1396 Fragments arranged by size: 2056 1810 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2086 3098 3615 5263 5450 1700 Size: 110 276 1012 517 1648 187 2869 Fragments arranged by size: 2869 1648 1012 517 276 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6619 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6619 BssSI C'ACGA_G Cuts at: 4679 5691 4679 Size: 1012 5607 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6619 BstBI TT'CG_AA Cuts at: 5423 5423 Size: 6619 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6619 BstYI r'GATC_y Cuts at: 1972 3949 4316 4701 4718 6170 6256 6268 6614 Size: 1977 367 385 17 1452 86 12 346 Cuts at: 6614 1972 Size: 1977 Fragments arranged by size: 1977 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2626 2868 2893 3357 3882 513 Size: 552 1561 242 25 464 525 3250 Fragments arranged by size: 3250 1561 552 525 464 242 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5880 Fragments arranged by size: 5880 683 56 BtsI GCAGTG_nn' Cuts at: 2396 4135 4929 4949 2396 Size: 1739 794 20 4066 Fragments arranged by size: 4066 1739 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6619 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2659 2694 529 Size: 35 2095 35 4454 Fragments arranged by size: 4454 2095 35 35 DraI TTT'AAA Cuts at: 3923 3923 Size: 6619 DraIII CAC_nnn'GTG Cuts at: 3409 3409 Size: 6619 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5626 809 Size: 507 4310 1802 Fragments arranged by size: 4310 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2871 3837 Size: 54 1425 50 275 18 207 834 966 Cuts at: 3837 4066 4955 6584 8 Size: 229 889 1629 43 Fragments arranged by size: 1629 1425 966 889 834 275 229 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2037 4066 1537 Size: 275 225 2029 4090 Fragments arranged by size: 4090 2029 275 225 EarI CTCTTCn'nnn_ Cuts at: 1353 2057 3052 3550 3861 4549 1353 Size: 704 995 498 311 688 3423 Fragments arranged by size: 3423 995 704 688 498 311 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5206 5580 5726 387 Size: 1064 138 3617 374 146 1280 Fragments arranged by size: 3617 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2854 2854 Size: 6619 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2291 2472 2554 2959 3052 3218 Size: 393 582 77 181 82 405 93 166 Cuts at: 3218 3536 3734 3776 3881 4682 5279 6066 6334 Size: 318 198 42 105 801 597 787 268 Cuts at: 6334 1239 Size: 1524 Fragments arranged by size: 1524 801 787 597 582 405 393 318 268 198 181 166 105 93 82 77 42 EcoNI CCTnn'n_nnAGG Cuts at: 2228 2228 Size: 6619 EcoO109I rG'GnC_Cy Cuts at: 2631 2812 2813 2844 3340 3427 2631 Size: 181 1 31 496 87 5823 Fragments arranged by size: 5823 496 181 87 31 1 EcoRI G'AATT_C Cuts at: 3928 4306 3928 Size: 378 6241 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2203 3664 2203 Size: 1461 5158 FspI TGC'GCA Cuts at: 5125 5125 Size: 6619 HaeII r_GCGC'y Cuts at: 1678 2805 3738 5766 1678 Size: 1127 933 2028 2531 Fragments arranged by size: 2531 2028 1127 933 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2503 2522 2535 2554 3604 Size: 32 135 32 931 19 13 19 1050 Cuts at: 3604 3620 3636 3652 3812 3844 5260 5292 5334 Size: 16 16 16 160 32 1416 32 42 Cuts at: 5334 5366 1373 Size: 32 2626 Fragments arranged by size: 2626 1416 1050 931 160 135 42 32 32 32 32 32 19 19 16 16 16 13 HincII GTy'rAC Cuts at: 669 1657 1938 3544 3963 4209 669 Size: 988 281 1606 419 246 3079 Fragments arranged by size: 3079 1606 988 419 281 246 HindIII A'AGCT_T Cuts at: 748 2180 748 Size: 1432 5187 HpaI GTT'AAC Cuts at: 4209 4209 Size: 6619 KpnI G_GTAC'C Cuts at: 3944 3944 Size: 6619 MfeI C'AATT_G Cuts at: 4218 4218 Size: 6619 MluI A'CGCG_T Cuts at: 6495 6495 Size: 6619 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2565 2665 5733 5917 1305 Size: 1260 100 3068 184 2007 Fragments arranged by size: 3068 2007 1260 184 100 MscI TGG'CCA Cuts at: 10 64 1489 1832 3839 10 Size: 54 1425 343 2007 2790 Fragments arranged by size: 2790 2007 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2363 2720 2915 3575 4577 4936 5095 Size: 648 1197 357 195 660 1002 359 159 Cuts at: 5095 518 Size: 2042 Fragments arranged by size: 2042 1197 1002 660 648 359 357 195 159 MspA1I CmG'CkG Cuts at: 1606 2269 2785 2808 2895 3161 3176 4035 4712 Size: 663 516 23 87 266 15 859 677 Cuts at: 4712 5860 6105 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 859 677 663 516 266 245 87 23 15 NaeI GCC'GGC Cuts at: 3617 3617 Size: 6619 NcoI C'CATG_G Cuts at: 513 1065 2868 3357 513 Size: 552 1803 489 3775 Fragments arranged by size: 3775 1803 552 489 NdeI CA'TA_TG Cuts at: 387 2775 387 Size: 2388 4231 NgoMIV G'CCGG_C Cuts at: 3615 3615 Size: 6619 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6619 NotI GC'GGCC_GC Cuts at: 2037 4066 2037 Size: 2029 4590 NspI r_CATG'y Cuts at: 1132 1229 2983 3232 3524 3977 5522 1132 Size: 97 1754 249 292 453 1545 2229 Fragments arranged by size: 2229 1754 1545 453 292 249 97 PasI CC'CwG_GG Cuts at: 1348 2283 1348 Size: 935 5684 PciI A'CATG_T Cuts at: 5518 5518 Size: 6619 PflMI CCAn_nnn'nTGG Cuts at: 1108 2982 3409 3449 1108 Size: 1874 427 40 4278 Fragments arranged by size: 4278 1874 427 40 PmeI GTTT'AAAC Cuts at: 3923 3923 Size: 6619 PmlI CAC'GTG Cuts at: 3681 3681 Size: 6619 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4688 4720 6201 6233 4688 Size: 32 1481 32 5074 Fragments arranged by size: 5074 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2631 3340 3427 2631 Size: 709 87 5823 Fragments arranged by size: 5823 709 87 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6619 PsiI TTA'TAA Cuts at: 4189 4189 Size: 6619 PspOMI G'GGCC_C Cuts at: 2812 2843 2812 Size: 31 6588 PstI C_TGCA'G Cuts at: 830 2865 3160 3971 830 Size: 2035 295 811 3478 Fragments arranged by size: 3478 2035 811 295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6619 PvuII CAG'CTG Cuts at: 2269 2785 2808 3161 3176 2269 Size: 516 23 353 15 5712 Fragments arranged by size: 5712 516 353 23 15 SacI G_AGCT'C Cuts at: 2856 2856 Size: 6619 SacII CC_GC'GG Cuts at: 2896 2896 Size: 6619 SalI G'TCGA_C Cuts at: 1936 3961 1936 Size: 2025 4594 SanDI GG'GwC_CC Cuts at: 2631 2631 Size: 6619 SapI GCTCTTCn'nnn_ Cuts at: 3052 3861 3052 Size: 809 5810 SbfI CC_TGCA'GG Cuts at: 3971 3971 Size: 6619 ScaI AGT'ACT Cuts at: 4867 6579 4867 Size: 1712 4907 SexAI A'CCwGG_T Cuts at: 3807 3807 Size: 6619 SfcI C'TryA_G Cuts at: 826 1045 2797 2861 3156 3967 5102 5783 5974 Size: 219 1752 64 295 811 1135 681 191 Cuts at: 5974 826 Size: 1471 Fragments arranged by size: 1752 1471 1135 811 681 295 219 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6619 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6619 SmaI CCC'GGG Cuts at: 1872 2849 3946 1872 Size: 977 1097 4545 Fragments arranged by size: 4545 1097 977 SmlI C'TyrA_G Cuts at: 820 1017 1943 4723 5624 5886 820 Size: 197 926 2780 901 262 1553 Fragments arranged by size: 2780 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4410 493 Size: 3917 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6619 SphI G_CATG'C Cuts at: 1132 3977 1132 Size: 2845 3774 SspI AAT'ATT Cuts at: 5 52 4543 5 Size: 47 4491 2081 Fragments arranged by size: 4491 2081 47 StuI AGG'CCT Cuts at: 1517 2759 1517 Size: 1242 5377 StyI C'CwwG_G Cuts at: 513 1065 2703 2868 3054 3357 3623 3639 4058 Size: 552 1638 165 186 303 266 16 419 Cuts at: 4058 513 Size: 3074 Fragments arranged by size: 3074 1638 552 419 303 266 186 165 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2334 4646 4663 4816 5001 5446 325 Size: 1255 754 2312 17 153 185 445 1498 Fragments arranged by size: 2312 1498 1255 754 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4865 6577 96 Size: 274 80 33 51 1443 2888 1712 138 Fragments arranged by size: 2888 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2623 2655 3405 3437 6579 6611 2623 Size: 32 750 32 3142 32 2631 Fragments arranged by size: 3142 2631 750 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3278 1316 Size: 1962 4657 XbaI T'CTAG_A Cuts at: 3955 3955 Size: 6619 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2290 2497 3364 3454 2290 Size: 207 867 90 5455 Fragments arranged by size: 5455 867 207 90 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6619 XmaI C'CCGG_G Cuts at: 1870 2847 3944 1870 Size: 977 1097 4545 Fragments arranged by size: 4545 1097 977 XmnI GAAnn'nnTTC Cuts at: 4748 4748 Size: 6619 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4427 276 Size: 53 83 186 936 2893 2468 Fragments arranged by size: 2893 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PspXI PsrI RsrII SfiI SfoI SrfI SwaI