Restriction map for
pFN21AB6142
(Linear) (Six-base) MAPSORT of: KIBB6142.seq Check: 8034 from: 1 to: 1914
KIBB6142.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1336 1914
Size: 1336 578
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 467 549 1047 1771 1914
Size: 209 258 82 498 724 143
Fragments arranged by size:
724 498 258 209 143 82
AflIII A'CryG_T
Cuts at: 0 911 1673 1914
Size: 911 762 241
Fragments arranged by size:
911 762 241
AgeI A'CCGG_T
Cuts at: 0 81 1914
Size: 81 1833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 1471 1914
Size: 733 738 443
Fragments arranged by size:
738 733 443
AlwNI CAG_nnn'CTG
Cuts at: 0 264 1233 1914
Size: 264 969 681
Fragments arranged by size:
969 681 264
ApaI G_GGCC'C
Cuts at: 0 811 842 1914
Size: 811 31 1072
Fragments arranged by size:
1072 811 31
ApoI r'AATT_y
Cuts at: 0 1309 1494 1914
Size: 1309 185 420
Fragments arranged by size:
1309 420 185
AvaI C'yCGr_G
Cuts at: 0 842 1087 1162 1691 1914
Size: 842 245 75 529 223
Fragments arranged by size:
842 529 245 223 75
BanI G'GyrC_C
Cuts at: 0 1348 1588 1696 1914
Size: 1348 240 108 218
Fragments arranged by size:
1348 240 218 108
BanII G_rGCy'C
Cuts at: 0 811 842 851 1914
Size: 811 31 9 1063
Fragments arranged by size:
1063 811 31 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 78 243 1691 1914
Size: 78 165 1448 223
Fragments arranged by size:
1448 223 165 78
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1281 1315 1914
Size: 1281 34 599
Fragments arranged by size:
1281 599 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 457 1914
Size: 457 1457
BclI T'GATC_A
Cuts at: 0 395 1914
Size: 395 1519
BglI GCCn_nnn'nGGC
Cuts at: 0 893 1099 1165 1914
Size: 893 206 66 749
Fragments arranged by size:
893 749 206 66
BlpI GC'TnA_GC
Cuts at: 0 74 1606 1914
Size: 74 1532 308
Fragments arranged by size:
1532 308 74
Bme1580I G_kGCm'C
Cuts at: 0 811 842 1591 1791 1914
Size: 811 31 749 200 123
Fragments arranged by size:
811 749 200 123 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 15 413 1914
Size: 15 398 1501
Fragments arranged by size:
1501 398 15
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 774 1056 1914
Size: 774 282 858
Fragments arranged by size:
858 774 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 954 1213 1531 1729 1876 1914
Size: 286 668 259 318 198 147 38
Fragments arranged by size:
668 318 286 259 198 147 38
BsaI GGTCTCn'nnnn_
Cuts at: 0 266 1550 1914
Size: 266 1284 364
Fragments arranged by size:
1284 364 266
BsaAI yAC'GTr
Cuts at: 0 912 1676 1914
Size: 912 764 238
Fragments arranged by size:
912 764 238
BsaBI GATnn'nnATC
Cuts at: 0 352 787 1914
Size: 352 435 1127
Fragments arranged by size:
1127 435 352
BsaHI Gr'CG_yC
Cuts at: 0 1475 1614 1688 1914
Size: 1475 139 74 226
Fragments arranged by size:
1475 226 139 74
BsaWI w'CCGG_w
Cuts at: 0 81 713 1914
Size: 81 632 1201
Fragments arranged by size:
1201 632 81
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 517 547 668 698 1400 1430 1914
Size: 517 30 121 30 702 30 484
Fragments arranged by size:
702 517 484 121 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 747 1568 1864 1914
Size: 747 821 296 50
Fragments arranged by size:
821 747 296 50
BseYI C'CCAG_C
Cuts at: 0 459 803 1713 1914
Size: 459 344 910 201
Fragments arranged by size:
910 459 344 201
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 656 1110 1438 1914
Size: 656 454 328 476
Fragments arranged by size:
656 476 454 328
BsiEI CG_ry'CG
Cuts at: 0 35 82 1914
Size: 35 47 1832
Fragments arranged by size:
1832 47 35
BsiHKAI G_wGCw'C
Cuts at: 0 851 1244 1914
Size: 851 393 670
Fragments arranged by size:
851 670 393
BsmI GAATG_Cn'
Cuts at: 0 1137 1914
Size: 1137 777
Bsp1286I G_dGCh'C
Cuts at: 0 811 842 851 1244 1591 1791 1914
Size: 811 31 9 393 347 200 123
Fragments arranged by size:
811 393 347 200 123 31 9
BspEI T'CCGG_A
Cuts at: 0 713 1914
Size: 713 1201
BspHI T'CATG_A
Cuts at: 0 734 1661 1914
Size: 734 927 253
Fragments arranged by size:
927 734 253
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1336 1914
Size: 1336 578
BsrDI GCAATG_nn'
Cuts at: 0 1299 1914
Size: 1299 615
BsrFI r'CCGG_y
Cuts at: 0 81 1093 1610 1914
Size: 81 1012 517 304
Fragments arranged by size:
1012 517 304 81
BtgI C'CryG_G
Cuts at: 0 621 863 888 1352 1877 1914
Size: 621 242 25 464 525 37
Fragments arranged by size:
621 525 464 242 37 25
BtsI GCAGTG_nn'
Cuts at: 0 391 1914
Size: 391 1523
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 654 689 1914
Size: 654 35 1225
Fragments arranged by size:
1225 654 35
DraIII CAC_nnn'GTG
Cuts at: 0 1404 1914
Size: 1404 510
EaeI y'GGCC_r
Cuts at: 0 32 866 1832 1914
Size: 32 834 966 82
Fragments arranged by size:
966 834 82 32
EagI C'GGCC_G
Cuts at: 0 32 1914
Size: 32 1882
EarI CTCTTCn'nnn_
Cuts at: 0 52 1047 1545 1856 1914
Size: 52 995 498 311 58
Fragments arranged by size:
995 498 311 58 52
EcoICRI GAG'CTC
Cuts at: 0 849 1914
Size: 849 1065
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 286 467 549 954 1047 1213 1531
Size: 209 77 181 82 405 93 166 318
Cuts at: 1531 1729 1771 1876 1914
Size: 198 42 105 38
Fragments arranged by size:
405 318 209 198 181 166 105 93
82 77 42 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 1914
Size: 223 1691
EcoO109I rG'GnC_Cy
Cuts at: 0 626 807 808 839 1335 1422 1914
Size: 626 181 1 31 496 87 492
Fragments arranged by size:
626 496 492 181 87 31 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 198 1659 1914
Size: 198 1461 255
Fragments arranged by size:
1461 255 198
HaeII r_GCGC'y
Cuts at: 0 800 1733 1914
Size: 800 933 181
Fragments arranged by size:
933 800 181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 498 517 530 549 1599 1615 1631 1647
Size: 498 19 13 19 1050 16 16 16
Cuts at: 1647 1807 1839 1914
Size: 160 32 75
Fragments arranged by size:
1050 498 160 75 32 19 19 16
16 16 13
HincII GTy'rAC
Cuts at: 0 1539 1914
Size: 1539 375
HindIII A'AGCT_T
Cuts at: 0 175 1914
Size: 175 1739
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 660 1914
Size: 560 100 1254
Fragments arranged by size:
1254 560 100
MscI TGG'CCA
Cuts at: 0 1834 1914
Size: 1834 80
MslI CAynn'nnrTG
Cuts at: 0 358 715 910 1570 1914
Size: 358 357 195 660 344
Fragments arranged by size:
660 358 357 344 195
MspA1I CmG'CkG
Cuts at: 0 264 780 803 890 1156 1171 1914
Size: 264 516 23 87 266 15 743
Fragments arranged by size:
743 516 266 264 87 23 15
NaeI GCC'GGC
Cuts at: 0 1612 1914
Size: 1612 302
NcoI C'CATG_G
Cuts at: 0 863 1352 1914
Size: 863 489 562
Fragments arranged by size:
863 562 489
NdeI CA'TA_TG
Cuts at: 0 770 1914
Size: 770 1144
NgoMIV G'CCGG_C
Cuts at: 0 1610 1914
Size: 1610 304
NotI GC'GGCC_GC
Cuts at: 0 32 1914
Size: 32 1882
NspI r_CATG'y
Cuts at: 0 978 1227 1519 1914
Size: 978 249 292 395
Fragments arranged by size:
978 395 292 249
PasI CC'CwG_GG
Cuts at: 0 278 1914
Size: 278 1636
PflMI CCAn_nnn'nTGG
Cuts at: 0 977 1404 1444 1914
Size: 977 427 40 470
Fragments arranged by size:
977 470 427 40
PmlI CAC'GTG
Cuts at: 0 1676 1914
Size: 1676 238
PpuMI rG'GwC_Cy
Cuts at: 0 626 1335 1422 1914
Size: 626 709 87 492
Fragments arranged by size:
709 626 492 87
PspOMI G'GGCC_C
Cuts at: 0 807 838 1914
Size: 807 31 1076
Fragments arranged by size:
1076 807 31
PstI C_TGCA'G
Cuts at: 0 860 1155 1914
Size: 860 295 759
Fragments arranged by size:
860 759 295
PvuII CAG'CTG
Cuts at: 0 264 780 803 1156 1171 1914
Size: 264 516 23 353 15 743
Fragments arranged by size:
743 516 353 264 23 15
SacI G_AGCT'C
Cuts at: 0 851 1914
Size: 851 1063
SacII CC_GC'GG
Cuts at: 0 891 1914
Size: 891 1023
SanDI GG'GwC_CC
Cuts at: 0 626 1914
Size: 626 1288
SapI GCTCTTCn'nnn_
Cuts at: 0 1047 1856 1914
Size: 1047 809 58
Fragments arranged by size:
1047 809 58
SexAI A'CCwGG_T
Cuts at: 0 1802 1914
Size: 1802 112
SfcI C'TryA_G
Cuts at: 0 792 856 1151 1914
Size: 792 64 295 763
Fragments arranged by size:
792 763 295 64
SmaI CCC'GGG
Cuts at: 0 844 1914
Size: 844 1070
StuI AGG'CCT
Cuts at: 0 754 1914
Size: 754 1160
StyI C'CwwG_G
Cuts at: 0 698 863 1049 1352 1618 1634 1914
Size: 698 165 186 303 266 16 280
Fragments arranged by size:
698 303 280 266 186 165 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 329 1914
Size: 329 1585
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 618 650 1400 1432 1914
Size: 618 32 750 32 482
Fragments arranged by size:
750 618 482 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1273 1914
Size: 1273 641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 285 492 1359 1449 1914
Size: 285 207 867 90 465
Fragments arranged by size:
867 465 285 207 90
XmaI C'CCGG_G
Cuts at: 0 842 1914
Size: 842 1072
Enzymes that do cut:
AarI AcuI AflIII AgeI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BcgI BciVI BclI BglI
BlpI Bme1580I BmrI BplI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BtgI
BtsI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII HindIII MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NotI
NspI PasI PflMI PmlI PpuMI PspOMI PstI PvuII
SacI SacII SanDI SapI SexAI SfcI SmaI StuI
StyI TaqII TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BfrBI BglII BmgBI BmtI Bpu10I BpuEI
BsiWI BsmBI BsrBI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraI
DrdI EciI EcoRI EcoRV FseI FspI FspAI HpaI
KasI KpnI MfeI MluI NarI NheI NruI NsiI
PacI PciI PfoI PmeI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SbfI ScaI SfiI SfoI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI XbaI XhoI XmnI ZraI