################################## # NO CUT ENZYMES ( 191 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BbvI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MboII MluI MluNI MroI MscI MseI MslI MspA1I MspI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TseI Tsp45I Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccII 290 CGCG CG^CG AcsI 130 GAATTT [GA]^AATT[CT] AfaI 72 GTAC GT^AC AlwI 93 GATCC GGATC(4/5) ApaI 83 GGGCCC GGGCC^C ApoI 130 GAATTT [GA]^AATT[CT] AspHI 308 GAGCTC G[AT]GC[AT]^C AvaI 212 CTCGAG C^[CT]CG[GA]G BanI 207 GGTGCC G^G[CT][GA]CC BfaI 256 CTAG C^TAG BsaJI 34 CCTGGG C^C[ACGT][ACGT]GG BsiHKAI 308 GAGCTC G[AT]GC[AT]^C BsmAI 259 GTCTC GTCTC(1/5) BsoBI 212 CTCGAG C^[CT]CG[GA]G BspMI 64 ACCTGC ACCTGC(4/8) BssKI 34 CCTGG ^CC[ACGT]GG BstF5I 244 GGATG GGATG(2/0) BstNI 34 CCTGG CC^[AT]GG BstUI 290 CGCG CG^CG BstYI 92 AGATCC [GA]^GATC[CT] DdeI 97 CTCAG C^T[ACGT]AG DraII 82 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoO109I 82 AGGGCCC [GA]G^G[ACGT]CC[CT] EcoRII 34 CCTGG ^CC[AT]GG EcoRV 139 GATATC GAT^ATC Fnu4HI 291 GCGGC GC^[ACGT]GC FokI 244 GGATG GGATG(9/13) HaeIII 84 GGCC GG^CC HgaI 288 GACGC GACGC(5/10) ItaI 291 GCGGC GC^[ACGT]GC MaeI 256 CTAG C^TAG MfeI 103 CAATTG C^AATTG MflI 92 AGATCC [GA]^GATC[CT] MunI 103 CAATTG C^AATTG MvaI 34 CCTGG CC^[AT]GG MvnI 290 CGCG CG^CG PaeR7I 212 CTCGAG C^TCGAG PleI 215 GAGTC GAGTC(4/5) RsaI 72 GTAC GT^AC SacI 308 GAGCTC GAGCT^C ScrFI 34 CCTGG CC^[ACGT]GG SfaNI 245 GATGC GCATC(5/9) TfiI 168 GATTC G^A[AT]TC TspRI 249 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoI 212 CTCGAG C^TCGAG XhoII 92 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AciI 242 GCGG CCGC(-3/-1) 291 GCGG AluI 188 AGCT AG^CT 309 AGCT BanII 83 GGGCCC G[GA]GC[CT]^C 308 GAGCTC BmyI 83 GGGCCC G[AGT]GC[ACT]^C 308 GAGCTC Bsp1286I 83 GGGCCC G[AGT]GC[ACT]^C 308 GAGCTC Cfr13I 83 GGGCC G^G[ACGT]CC 84 GGCCC DpnII 93 GATC ^GATC 253 GATC HinfI 168 GATTC G^A[ACGT]TC 215 GAGTC MboI 93 GATC ^GATC 253 GATC NdeII 93 GATC ^GATC 253 GATC NlaIV 83 GGGCCC GG[ACGT]^[ACGT]CC 207 GGTGCC PstI 75 CTGCAG CTGCA^G 313 CTGCAG Sau3AI 93 GATC ^GATC 253 GATC Sau96I 83 GGGCC G^G[ACGT]CC 84 GGCCC SfcI 75 CTGCAG C^T[GA][CT]AG 313 CTGCAG SmlI 5 CTCAAG C^T[CT][GA]AG 212 CTCGAG TaqI 137 TCGA T^CGA 213 TCGA Tsp509I 104 AATT ^AATT 131 AATT TthHB8I 137 TCGA T^CGA 213 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 2 enzymes) # ######################################### MnlI MwoI