################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanII BbeI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BspDI BspEI BspHI BspMI BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MspA1I MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SwaI Tsp45I Tth111I XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AflII 634 CTTAAG C^TTAAG AseI 895 ATTAAT AT^TAAT AsnI 895 ATTAAT AT^TAAT BanI 114 GGTGCC G^G[CT][GA]CC BbrPI 244 CACGTG CAC^GTG BbsI 461 GTCTTC GAAGAC(2/6) BfaI 715 CTAG C^TAG BfrI 634 CTTAAG C^TTAAG BmyI 115 GTGCCC G[AGT]GC[ACT]^C BpuAI 461 GTCTTC GAAGAC(2/6) BsaAI 244 CACGTG [CT]AC^GT[GA] BsmI 643 GAATGC GAATGC(1/-1) Bsp1286I 115 GTGCCC G[AGT]GC[ACT]^C Bsp1407I 742 TGTACA T^GTACA BspLU11I 415 ACATGT A^CATGT BsrDI 1003 CATTGC GCAATG(2/0) BsrFI 55 GCCGGC [GA]^CCGG[CT] BsrGI 742 TGTACA T^GTACA BsrI 205 CCAGT ACTGG(1/-1) BstAPI 261 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 300 CCAATGGTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 25 GCGC GCG^C Cfr10I 55 GCCGGC [GA]^CCGG[CT] DdeI 950 CTGAG C^T[ACGT]AG DraII 729 AGGACCC [GA]G^G[ACGT]CC[CT] EcoO109I 729 AGGACCC [GA]G^G[ACGT]CC[CT] EcoRI 998 GAATTC G^AATTC EcoRV 527 GATATC GAT^ATC EcoT14I 15 CCTTGG C^C[AT][AT]GG HhaI 25 GCGC GCG^C HinP1I 25 GCGC G^CGC MaeI 715 CTAG C^TAG MaeIII 256 GTAAC ^GT[ACGT]AC NaeI 55 GCCGGC GCC^GGC NgoMIV 55 GCCGGC G^CCGGC NspI 415 ACATGT [GA]CATG^[CT] PflMI 910 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 237 GAGTC GAGTC(4/5) PmaCI 244 CACGTG CAC^GTG PmlI 244 CACGTG CAC^GTG PpuMI 729 AGGACCC [GA]G^G[AT]CC[CT] PshBI 895 ATTAAT AT^TAAT PstI 966 CTGCAG CTGCA^G SfcI 966 CTGCAG C^T[GA][CT]AG SmaI 47 CCCGGG CCC^GGG SmlI 634 CTTAAG C^T[CT][GA]AG SspBI 742 TGTACA T^GTACA SspI 559 AATATT AAT^ATT StuI 5 AGGCCT AGG^CCT StyI 15 CCTTGG C^C[AT][AT]GG TfiI 172 GATTC G^A[AT]TC Van91I 910 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 714 TCTAGA T^CTAGA XcmI 292 CCATCATTCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 47 CCCGGG C^CCGGG XmaI 47 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 26 CGCG CG^CG 652 CGCG AfaI 743 GTAC GT^AC 875 GTAC AlwI 190 GGATC GGATC(4/5) 976 GGATC AvaI 47 CCCGGG C^[CT]CG[GA]G 71 CTCGGG AvaII 44 GGACC G^G[AT]CC 730 GGACC BcnI 47 CCCGG CC^[GC]GG 48 CCGGG BseRI 85 CTCCTC GAGGAG(10/8) 391 GAGGAG BsgI 347 CTGCAC GTGCAG(16/14) 839 GTGCAG BsoBI 47 CCCGGG C^[CT]CG[GA]G 71 CTCGGG BstUI 26 CGCG CG^CG 652 CGCG Cac8I 35 GCAGGC GC[ACGT]^[ACGT]GC 55 GCCGGC FauI 30 GCGGG CCCGC(4/6) 111 GCGGG HapII 48 CCGG C^CGG 56 CCGG HinfI 172 GATTC G^A[ACGT]TC 237 GAGTC HpaII 48 CCGG C^CGG 56 CCGG MslI 296 CATTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 751 CATAACTGTG MspI 48 CCGG C^CGG 56 CCGG MvnI 26 CGCG CG^CG 652 CGCG NciI 47 CCCGG CC^[GC]GG 48 CCGGG RsaI 743 GTAC GT^AC 875 GTAC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AciI AcsI AflIII AluI ApoI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I DpnII EcoRII Fnu4HI FokI HaeIII ItaI MaeII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TaqI Tru9I TseI Tsp509I TspRI TthHB8I