(Circular) (Six-base) MAPSORT of: pFN21AE6350.seq Check: 6604 from: 1 to: 5758 pFN21AE6350 5758 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3568 278 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3101 1937 Size: 1164 4594 Acc65I G'GTAC_C Cuts at: 3079 3079 Size: 5758 AclI AA'CG_TT Cuts at: 1563 3885 4258 1563 Size: 2322 373 3063 Fragments arranged by size: 3063 2322 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2573 3821 5205 5473 1632 Size: 941 1248 1384 268 1917 Fragments arranged by size: 1917 1384 1248 941 268 AflII C'TTAA_G Cuts at: 820 1017 2639 820 Size: 197 1622 3939 Fragments arranged by size: 3939 1622 197 AflIII A'CryG_T Cuts at: 1203 2248 2250 2420 4657 5634 1203 Size: 1045 2 170 2237 977 1327 Fragments arranged by size: 2237 1327 1045 977 170 2 AgeI A'CCGG_T Cuts at: 4589 4589 Size: 5758 AhdI GACnn_n'nnGTC Cuts at: 4487 4487 Size: 5758 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2563 3067 1184 Size: 1379 504 3875 Fragments arranged by size: 3875 1379 504 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2127 2159 2127 Size: 32 5726 AlwNI CAG_nnn'CTG Cuts at: 3501 5073 3501 Size: 1572 4186 ApaLI G'TGCA_C Cuts at: 3815 4971 3815 Size: 1156 4602 ApoI r'AATT_y Cuts at: 1493 2389 2412 2628 2829 3003 3067 3293 3445 Size: 896 23 216 201 174 64 226 152 Cuts at: 3445 5554 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 896 226 216 201 174 152 64 23 AseI AT'TA_AT Cuts at: 160 1032 2901 4312 160 Size: 872 1869 1411 1606 Fragments arranged by size: 1869 1606 1411 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 AvaI C'yCGr_G Cuts at: 1870 1943 2052 2076 3083 1870 Size: 73 109 24 1007 4545 Fragments arranged by size: 4545 1007 109 73 24 BamHI G'GATC_C Cuts at: 3088 3455 3088 Size: 367 5391 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 3079 4534 618 Size: 325 217 627 332 960 1455 1842 Fragments arranged by size: 1842 1455 960 627 332 325 217 BanII G_rGCy'C Cuts at: 1391 3077 1391 Size: 1686 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2459 928 Size: 971 560 4227 Fragments arranged by size: 4227 971 560 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3949 3983 1903 Size: 34 2012 34 3678 Fragments arranged by size: 3678 2012 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3650 4860 3650 Size: 1210 4548 BclI T'GATC_A Cuts at: 1548 1548 Size: 5758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4369 136 Size: 107 122 71 631 605 337 2360 1525 Fragments arranged by size: 2360 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5753 5753 Size: 5758 BlpI GC'TnA_GC Cuts at: 3175 3175 Size: 5758 Bme1580I G_kGCm'C Cuts at: 1790 2124 3819 4975 1790 Size: 334 1695 1156 2573 Fragments arranged by size: 2573 1695 1156 334 BmgBI CAC'GTC Cuts at: 1318 2843 1318 Size: 1525 4233 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3489 4447 442 Size: 416 53 83 399 24 2072 958 1753 Fragments arranged by size: 2072 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5758 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4418 1239 Size: 3179 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3883 4748 5046 3883 Size: 865 298 4595 Fragments arranged by size: 4595 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4421 882 Size: 617 2922 2219 Fragments arranged by size: 2922 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2251 3549 493 Size: 711 1047 1298 2702 Fragments arranged by size: 2702 1298 1047 711 BsaBI GATnn'nnATC Cuts at: 5752 5752 Size: 5758 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3565 3947 4582 275 Size: 53 83 186 936 2032 382 635 1451 Fragments arranged by size: 2032 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4191 4589 4863 5010 5421 4191 Size: 398 274 147 411 4528 Fragments arranged by size: 4528 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5533 Fragments arranged by size: 5533 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 2411 1186 Size: 895 330 4533 Fragments arranged by size: 4533 895 330 BseYI C'CCAG_C Cuts at: 2026 4961 5457 2026 Size: 2935 496 2327 Fragments arranged by size: 2935 2327 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2337 2865 1163 Size: 573 601 528 4056 Fragments arranged by size: 4056 601 573 528 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3208 3969 4997 1540 Size: 275 186 1207 761 1028 2301 Fragments arranged by size: 2301 1207 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3819 3904 4975 3819 Size: 85 1071 4602 Fragments arranged by size: 4602 1071 85 BsmI GAATG_Cn' Cuts at: 2654 3269 3362 2654 Size: 615 93 5050 Fragments arranged by size: 5050 615 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2124 3077 3819 3904 4975 1391 Size: 399 334 953 742 85 1071 2174 Fragments arranged by size: 2174 1071 953 742 399 334 85 BspEI T'CCGG_A Cuts at: 5421 5421 Size: 5758 BspHI T'CATG_A Cuts at: 3647 5377 3647 Size: 1730 4028 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3113 844 Size: 2269 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3645 1394 Size: 429 1822 3507 Fragments arranged by size: 3507 1822 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3007 4253 4427 65 Size: 1183 1759 1246 174 1396 Fragments arranged by size: 1759 1396 1246 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2060 4402 4589 1700 Size: 110 250 2342 187 2869 Fragments arranged by size: 2869 2342 250 187 110 BsrGI T'GTAC_A Cuts at: 96 2747 96 Size: 2651 3107 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5758 BssSI C'ACGA_G Cuts at: 3818 4830 3818 Size: 1012 4746 BstAPI GCAn_nnn'nTGC Cuts at: 794 2272 794 Size: 1478 4280 BstBI TT'CG_AA Cuts at: 4562 4562 Size: 5758 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5758 BstXI CCAn_nnnn'nTGG Cuts at: 2312 2312 Size: 5758 BstYI r'GATC_y Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753 Size: 1116 367 385 17 1452 86 12 346 Cuts at: 5753 1972 Size: 1977 Fragments arranged by size: 1977 1452 1116 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2639 2758 487 Size: 683 56 1413 119 3487 Fragments arranged by size: 3487 1413 683 119 56 BtsI GCAGTG_nn' Cuts at: 2349 3274 4068 4088 2349 Size: 925 794 20 4019 Fragments arranged by size: 4019 925 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5758 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5723 DraI TTT'AAA Cuts at: 3062 3062 Size: 5758 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4765 809 Size: 507 3449 1802 Fragments arranged by size: 3449 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3205 4094 5723 Size: 54 1425 50 275 18 1375 889 1629 Cuts at: 5723 8 Size: 43 Fragments arranged by size: 1629 1425 1375 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3205 1537 Size: 275 1393 4090 Fragments arranged by size: 4090 1393 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3688 1353 Size: 2335 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2062 2092 4345 4719 4865 387 Size: 1064 138 473 30 2253 374 146 1280 Fragments arranged by size: 2253 1280 1064 473 374 146 138 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2573 3821 4418 5205 5473 1239 Size: 393 941 1248 597 787 268 1524 Fragments arranged by size: 1524 1248 941 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2735 2735 Size: 5758 EcoRI G'AATT_C Cuts at: 3003 3067 3445 3003 Size: 64 378 5316 Fragments arranged by size: 5316 378 64 EcoRV GAT'ATC Cuts at: 2534 2534 Size: 5758 FspI TGC'GCA Cuts at: 4264 4264 Size: 5758 HaeII r_GCGC'y Cuts at: 1678 4905 1678 Size: 3227 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4399 4431 4473 4505 1373 Size: 32 135 32 2827 32 42 32 2626 Fragments arranged by size: 2827 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3102 3348 669 Size: 988 281 1164 246 3079 Fragments arranged by size: 3079 1164 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5758 HpaI GTT'AAC Cuts at: 3348 3348 Size: 5758 KpnI G_GTAC'C Cuts at: 3083 3083 Size: 5758 MfeI C'AATT_G Cuts at: 3357 3357 Size: 5758 MluI A'CGCG_T Cuts at: 5634 5634 Size: 5758 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 4872 5056 1305 Size: 808 2759 184 2007 Fragments arranged by size: 2759 2007 808 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3936 Fragments arranged by size: 3936 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2305 2760 3716 4075 4234 518 Size: 648 1139 455 956 359 159 2042 Fragments arranged by size: 2042 1139 956 648 455 359 159 MspA1I CmG'CkG Cuts at: 1606 3174 3851 4999 5244 1606 Size: 1568 677 1148 245 2120 Fragments arranged by size: 2120 1568 1148 677 245 NaeI GCC'GGC Cuts at: 2062 2062 Size: 5758 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4267 Fragments arranged by size: 4267 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5758 NgoMIV G'CCGG_C Cuts at: 2060 2060 Size: 5758 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5758 NotI GC'GGCC_GC Cuts at: 3205 3205 Size: 5758 NspI r_CATG'y Cuts at: 1132 1229 2424 3116 4661 1132 Size: 97 1195 692 1545 2229 Fragments arranged by size: 2229 1545 1195 692 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5758 PciI A'CATG_T Cuts at: 2420 4657 2420 Size: 2237 3521 PflMI CCAn_nnn'nTGG Cuts at: 1108 2921 1108 Size: 1813 3945 PfoI T'CCnGG_A Cuts at: 2914 2914 Size: 5758 PmeI GTTT'AAAC Cuts at: 3062 3062 Size: 5758 PmlI CAC'GTG Cuts at: 2251 2251 Size: 5758 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3827 3859 5340 5372 3827 Size: 32 1481 32 4213 Fragments arranged by size: 4213 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2735 2735 Size: 5758 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5758 PsiI TTA'TAA Cuts at: 2562 3328 2562 Size: 766 4992 PstI C_TGCA'G Cuts at: 830 2975 3110 830 Size: 2145 135 3478 Fragments arranged by size: 3478 2145 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5758 SalI G'TCGA_C Cuts at: 1936 3100 1936 Size: 1164 4594 SbfI CC_TGCA'GG Cuts at: 3110 3110 Size: 5758 ScaI AGT'ACT Cuts at: 4006 5718 4006 Size: 1712 4046 SfcI C'TryA_G Cuts at: 826 1045 2971 3106 4241 4922 5113 826 Size: 219 1926 135 1135 681 191 1471 Fragments arranged by size: 1926 1471 1135 681 219 191 135 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5758 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5758 SmaI CCC'GGG Cuts at: 1872 2054 3085 1872 Size: 182 1031 4545 Fragments arranged by size: 4545 1031 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 2639 3862 4763 5025 820 Size: 197 926 696 1223 901 262 1553 Fragments arranged by size: 1553 1223 926 901 696 262 197 SnaBI TAC'GTA Cuts at: 493 3549 493 Size: 3056 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5758 SphI G_CATG'C Cuts at: 1132 3116 1132 Size: 1984 3774 SspI AAT'ATT Cuts at: 5 52 2566 3682 5 Size: 47 2514 1116 2081 Fragments arranged by size: 2514 2081 1116 47 StuI AGG'CCT Cuts at: 1517 2012 1517 Size: 495 5263 StyI C'CwwG_G Cuts at: 513 1065 2004 2020 3197 513 Size: 552 939 16 1177 3074 Fragments arranged by size: 3074 1177 939 552 16 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3785 3802 3955 4140 4585 325 Size: 1255 2205 17 153 185 445 1498 Fragments arranged by size: 2205 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2747 4004 5716 Size: 274 80 33 51 1443 770 1257 1712 Cuts at: 5716 96 Size: 138 Fragments arranged by size: 1712 1443 1257 770 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5718 5750 5718 Size: 32 5726 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5758 XbaI T'CTAG_A Cuts at: 2719 3094 2719 Size: 375 5383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2304 2304 Size: 5758 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5758 XmaI C'CCGG_G Cuts at: 1870 2052 3083 1870 Size: 182 1031 4545 Fragments arranged by size: 4545 1031 182 XmnI GAAnn'nnTTC Cuts at: 3887 3887 Size: 5758 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3566 276 Size: 53 83 186 936 2032 2468 Fragments arranged by size: 2468 2032 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI