################################## # NO CUT ENZYMES ( 144 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I AspEI AspI AvaII AvrII BalI BamHI BbeI BbrPI BcgI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BspDI BspEI BspHI BspLU11I BspMI BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FseI HaeII HgaI Hin1I HindIII HpaI KasI KspI MamI MluI MluNI MroI MscI MslI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 1203 CCGC CCGC(-3/-1) AflIII 1237 ACGTGT A^C[GA][CT]GT AseI 616 ATTAAT AT^TAAT AsnI 616 ATTAAT AT^TAAT AspHI 1111 GAGCTC G[AT]GC[AT]^C AvaI 187 CCCGAG C^[CT]CG[GA]G AviII 1017 TGCGCA TGC^GCA BanII 1111 GAGCTC G[GA]GC[CT]^C BbvCI 909 CCTCAGC CCTCAGC(-5/-2) BciVI 50 GTATCC GTATCC(6/5) BclI 890 TGATCA T^GATCA BglII 1190 AGATCT A^GATCT BsaAI 856 TACGTA [CT]AC^GT[GA] BsiHKAI 1111 GAGCTC G[AT]GC[AT]^C BsoBI 187 CCCGAG C^[CT]CG[GA]G Bsp1407I 777 TGTACA T^GTACA BsrDI 1047 CATTGC GCAATG(2/0) BsrFI 34 ACCGGC [GA]^CCGG[CT] BsrGI 777 TGTACA T^GTACA Cac8I 823 GCTCGC GC[ACGT]^[ACGT]GC Cfr10I 34 ACCGGC [GA]^CCGG[CT] Cfr13I 882 GGGCC G^G[ACGT]CC EarI 206 GAAGAG CTCTTC(1/4) EcoRV 991 GATATC GAT^ATC FauI 1202 CCCGC CCCGC(4/6) FbaI 890 TGATCA T^GATCA FspI 1017 TGCGCA TGC^GCA HapII 35 CCGG C^CGG HincII 220 GTTGAC GT[CT]^[GA]AC HindII 220 GTTGAC GT[CT]^[GA]AC HpaII 35 CCGG C^CGG Ksp632I 206 GAAGAG CTCTTC(1/4) MfeI 1124 CAATTG C^AATTG MspA1I 256 CAGCTG C[AC]G^C[GT]G MspI 35 CCGG C^CGG MunI 1124 CAATTG C^AATTG MwoI 905 GCATCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 679 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 190 GAGTC GAGTC(4/5) PshBI 616 ATTAAT AT^TAAT PvuII 256 CAGCTG CAG^CTG SacI 1111 GAGCTC GAGCT^C Sau96I 882 GGGCC G^G[ACGT]CC SfcI 1052 CTATAG C^T[GA][CT]AG SnaBI 856 TACGTA TAC^GTA SspBI 777 TGTACA T^GTACA Van91I 679 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 233 CCAAGTTCCTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### Acc65I 739 GGTACC G^GTACC 1161 GGTACC Asp718I 739 GGTACC G^GTACC 1161 GGTACC BbsI 492 GAAGAC GAAGAC(2/6) 1330 GAAGAC BfaI 386 CTAG C^TAG 430 CTAG BmyI 30 GGGCAC G[AGT]GC[ACT]^C 1111 GAGCTC BpuAI 492 GAAGAC GAAGAC(2/6) 1330 GAAGAC BseRI 7 GAGGAG GAGGAG(10/8) 119 GAGGAG BsiYI 109 CCTTACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 679 CCACTCTATGG BslI 109 CCTTACTACGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 679 CCACTCTATGG BsmAI 209 GAGAC GTCTC(1/5) 1231 GAGAC Bsp1286I 30 GGGCAC G[AGT]GC[ACT]^C 1111 GAGCTC BsrI 212 ACTGG ACTGG(1/-1) 553 ACTGG BstF5I 906 CATCC GGATG(2/0) 1268 GGATG BstYI 459 AGATCC [GA]^GATC[CT] 1190 AGATCT CfoI 1018 GCGC GCG^C 1305 GCGC DraI 166 TTTAAA TTT^AAA 304 TTTAAA FokI 906 CATCC GGATG(9/13) 1268 GGATG HhaI 1018 GCGC GCG^C 1305 GCGC HinP1I 1018 GCGC G^CGC 1305 GCGC HphI 888 GGTGA GGTGA(8/7) 181 TCACC KpnI 739 GGTACC GGTAC^C 1161 GGTACC MaeI 386 CTAG C^TAG 430 CTAG MflI 459 AGATCC [GA]^GATC[CT] 1190 AGATCT NdeI 94 CATATG CA^TATG 1208 CATATG NlaIII 559 CATG CATG^ 1005 CATG TfiI 228 GATTC G^A[AT]TC 640 GAATC XhoII 459 AGATCC [GA]^GATC[CT] 1190 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BanI BbvI BsaJI BssKI BstNI DdeI DpnII EcoRII EcoT14I Fnu4HI HaeIII HinfI ItaI MaeII MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIV RsaI Sau3AI ScrFI SfaNI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I