(Circular) (Six-base) MAPSORT of: pFN21AE3905.seq Check: 4347 from: 1 to: 6040 pFN21AE3905 6040 bp With 182 enzymes: SgfI * March 28, 2012 11:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3850 278 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3383 1937 Size: 1446 4594 Acc65I G'GTAC_C Cuts at: 2744 3166 3361 2744 Size: 422 195 5423 Fragments arranged by size: 5423 422 195 AclI AA'CG_TT Cuts at: 1563 4167 4540 1563 Size: 2604 373 3063 Fragments arranged by size: 3063 2604 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2340 2391 2407 2835 4103 5487 5755 1632 Size: 708 51 16 428 1268 1384 268 1917 Fragments arranged by size: 1917 1384 1268 708 428 268 51 16 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5843 AflIII A'CryG_T Cuts at: 1203 3242 4939 5916 1203 Size: 2039 1697 977 1327 Fragments arranged by size: 2039 1697 1327 977 AgeI A'CCGG_T Cuts at: 4871 4871 Size: 6040 AhdI GACnn_n'nnGTC Cuts at: 4769 4769 Size: 6040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6040 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6040 AlwNI CAG_nnn'CTG Cuts at: 3783 5355 3783 Size: 1572 4468 ApaLI G'TGCA_C Cuts at: 4097 5253 4097 Size: 1156 4884 ApoI r'AATT_y Cuts at: 1493 2429 2525 3140 3349 3575 3727 5836 1493 Size: 936 96 615 209 226 152 2109 1697 Fragments arranged by size: 2109 1697 936 615 226 209 152 96 AseI AT'TA_AT Cuts at: 160 1032 2622 4594 160 Size: 872 1590 1972 1606 Fragments arranged by size: 1972 1606 1590 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 AvaI C'yCGr_G Cuts at: 1870 1943 2192 3365 1870 Size: 73 249 1173 4545 Fragments arranged by size: 4545 1173 249 73 BamHI G'GATC_C Cuts at: 3370 3737 3370 Size: 367 5673 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2036 2744 3166 3361 4816 Size: 325 217 627 249 708 422 195 1455 Cuts at: 4816 618 Size: 1842 Fragments arranged by size: 1842 1455 708 627 422 325 249 217 195 BanII G_rGCy'C Cuts at: 1391 3120 3359 1391 Size: 1729 239 4072 Fragments arranged by size: 4072 1729 239 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2504 3342 928 Size: 971 605 838 3626 Fragments arranged by size: 3626 971 838 605 BbvCI CC'TCA_GC Cuts at: 2915 2915 Size: 6040 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4231 4265 1903 Size: 34 2294 34 3678 Fragments arranged by size: 3678 2294 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2066 3932 5142 2066 Size: 1866 1210 2964 Fragments arranged by size: 2964 1866 1210 BclI T'GATC_A Cuts at: 1548 2895 1548 Size: 1347 4693 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4651 136 Size: 107 122 71 631 605 2979 1525 Fragments arranged by size: 2979 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3195 6035 3195 Size: 2840 3200 BlpI GC'TnA_GC Cuts at: 3457 3457 Size: 6040 Bme1580I G_kGCm'C Cuts at: 1790 2039 4101 5257 1790 Size: 249 2062 1156 2573 Fragments arranged by size: 2573 2062 1156 249 BmgBI CAC'GTC Cuts at: 1318 2023 1318 Size: 705 5335 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3771 4729 442 Size: 416 53 83 399 24 2354 958 1753 Fragments arranged by size: 2354 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6040 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4700 1239 Size: 3461 2579 Bpu10I CC'TnA_GC Cuts at: 2915 2915 Size: 6040 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4165 5030 5328 4165 Size: 865 298 4877 Fragments arranged by size: 4877 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4703 882 Size: 617 3204 2219 Fragments arranged by size: 3204 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2863 3831 493 Size: 711 1659 968 2702 Fragments arranged by size: 2702 1659 968 711 BsaBI GATnn'nnATC Cuts at: 6034 6034 Size: 6040 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3847 4229 4864 275 Size: 53 83 186 936 2314 382 635 1451 Fragments arranged by size: 2314 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4473 4871 5145 5292 5703 4473 Size: 398 274 147 411 4810 Fragments arranged by size: 4810 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5815 Fragments arranged by size: 5815 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 2139 1186 Size: 841 112 5087 Fragments arranged by size: 5087 841 112 BseYI C'CCAG_C Cuts at: 2032 2855 5243 5739 2032 Size: 823 2388 496 2333 Fragments arranged by size: 2388 2333 823 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5467 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3490 4251 5279 1540 Size: 275 186 1489 761 1028 2301 Fragments arranged by size: 2301 1489 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3120 4101 4186 5257 3120 Size: 981 85 1071 3903 Fragments arranged by size: 3903 1071 981 85 BsmI GAATG_Cn' Cuts at: 3551 3644 3551 Size: 93 5947 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2039 3120 3359 4101 4186 5257 1391 Size: 399 249 1081 239 742 85 1071 2174 Fragments arranged by size: 2174 1081 1071 742 399 249 239 85 BspEI T'CCGG_A Cuts at: 5703 5703 Size: 6040 BspHI T'CATG_A Cuts at: 3929 5659 3929 Size: 1730 4310 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3395 844 Size: 2551 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3927 1394 Size: 429 2104 3507 Fragments arranged by size: 3507 2104 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3051 4535 4709 65 Size: 1183 1803 1484 174 1396 Fragments arranged by size: 1803 1484 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 4684 4871 1700 Size: 110 229 2645 187 2869 Fragments arranged by size: 2869 2645 229 187 110 BsrGI T'GTAC_A Cuts at: 96 2782 96 Size: 2686 3354 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6040 BssSI C'ACGA_G Cuts at: 4100 5112 4100 Size: 1012 5028 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6040 BstBI TT'CG_AA Cuts at: 4844 4844 Size: 6040 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6040 BstYI r'GATC_y Cuts at: 1972 2464 3195 3370 3737 4122 4139 5591 5677 Size: 492 731 175 367 385 17 1452 86 Cuts at: 5677 5689 6035 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 731 492 385 367 346 175 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5488 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5301 Fragments arranged by size: 5301 683 56 BtsI GCAGTG_nn' Cuts at: 3556 4350 4370 3556 Size: 794 20 5226 Fragments arranged by size: 5226 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6040 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3214 3249 529 Size: 35 2650 35 3320 Fragments arranged by size: 3320 2650 35 35 DraI TTT'AAA Cuts at: 2173 2311 3344 2173 Size: 138 1033 4869 Fragments arranged by size: 4869 1033 138 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5047 809 Size: 507 3731 1802 Fragments arranged by size: 3731 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3487 4376 6005 Size: 54 1425 50 275 18 1657 889 1629 Cuts at: 6005 8 Size: 43 Fragments arranged by size: 1657 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3487 1537 Size: 275 1675 4090 Fragments arranged by size: 4090 1675 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2206 3970 1353 Size: 853 1764 3423 Fragments arranged by size: 3423 1764 853 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4627 5001 5147 387 Size: 1064 138 3038 374 146 1280 Fragments arranged by size: 3038 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3118 3118 Size: 6040 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2340 2391 2407 2835 4103 4700 5487 Size: 393 708 51 16 428 1268 597 787 Cuts at: 5487 5755 1239 Size: 268 1524 Fragments arranged by size: 1524 1268 787 708 597 428 393 268 51 16 EcoRI G'AATT_C Cuts at: 3349 3727 3349 Size: 378 5662 EcoRV GAT'ATC Cuts at: 2998 2998 Size: 6040 FspI TGC'GCA Cuts at: 3024 4546 3024 Size: 1522 4518 HaeII r_GCGC'y Cuts at: 1678 5187 1678 Size: 3509 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2120 2152 2933 2965 4681 Size: 32 135 32 548 32 781 32 1716 Cuts at: 4681 4713 4755 4787 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1716 781 548 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 3384 3630 669 Size: 988 281 289 1157 246 3079 Fragments arranged by size: 3079 1157 988 289 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6040 HpaI GTT'AAC Cuts at: 3630 3630 Size: 6040 KpnI G_GTAC'C Cuts at: 2748 3170 3365 2748 Size: 422 195 5423 Fragments arranged by size: 5423 422 195 MfeI C'AATT_G Cuts at: 3129 3639 3129 Size: 510 5530 MluI A'CGCG_T Cuts at: 5916 5916 Size: 6040 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5154 5338 1305 Size: 3849 184 2007 Fragments arranged by size: 3849 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4218 Fragments arranged by size: 4218 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3998 4357 4516 518 Size: 648 2832 359 159 2042 Fragments arranged by size: 2832 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2263 3456 4133 5281 5526 1606 Size: 657 1193 677 1148 245 2120 Fragments arranged by size: 2120 1193 1148 677 657 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5488 NdeI CA'TA_TG Cuts at: 387 2100 3214 387 Size: 1713 1114 3213 Fragments arranged by size: 3213 1713 1114 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6040 NotI GC'GGCC_GC Cuts at: 3487 3487 Size: 6040 NspI r_CATG'y Cuts at: 1132 1229 3398 4943 1132 Size: 97 2169 1545 2229 Fragments arranged by size: 2229 2169 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6040 PciI A'CATG_T Cuts at: 4939 4939 Size: 6040 PflMI CCAn_nnn'nTGG Cuts at: 1108 2690 1108 Size: 1582 4458 PmeI GTTT'AAAC Cuts at: 3344 3344 Size: 6040 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4109 4141 5622 5654 4109 Size: 32 1481 32 4495 Fragments arranged by size: 4495 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6040 PsiI TTA'TAA Cuts at: 3610 3610 Size: 6040 PstI C_TGCA'G Cuts at: 830 3392 830 Size: 2562 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6040 PvuII CAG'CTG Cuts at: 2263 2263 Size: 6040 SacI G_AGCT'C Cuts at: 3120 3120 Size: 6040 SalI G'TCGA_C Cuts at: 1936 3382 1936 Size: 1446 4594 SbfI CC_TGCA'GG Cuts at: 3392 3392 Size: 6040 ScaI AGT'ACT Cuts at: 4288 6000 4288 Size: 1712 4328 SfcI C'TryA_G Cuts at: 826 1045 3057 3388 4523 5204 5395 826 Size: 219 2012 331 1135 681 191 1471 Fragments arranged by size: 2012 1471 1135 681 331 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6040 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6040 SmaI CCC'GGG Cuts at: 1872 3367 1872 Size: 1495 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4144 5045 5307 820 Size: 197 926 2201 901 262 1553 Fragments arranged by size: 2201 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2863 3831 493 Size: 2370 968 2702 Fragments arranged by size: 2702 2370 968 SpeI A'CTAG_T Cuts at: 152 152 Size: 6040 SphI G_CATG'C Cuts at: 1132 3398 1132 Size: 2266 3774 SspI AAT'ATT Cuts at: 5 52 3964 5 Size: 47 3912 2081 Fragments arranged by size: 3912 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6040 StyI C'CwwG_G Cuts at: 513 1065 2695 2710 3081 3479 513 Size: 552 1630 15 371 398 3074 Fragments arranged by size: 3074 1630 552 398 371 15 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4067 4084 4237 4422 4867 325 Size: 1255 2487 17 153 185 445 1498 Fragments arranged by size: 2487 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2782 3048 3065 Size: 274 80 33 51 1443 805 266 17 Cuts at: 3065 4286 5998 96 Size: 1221 1712 138 Fragments arranged by size: 1712 1443 1221 805 274 266 138 80 51 33 17 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6000 6032 6000 Size: 32 6008 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6040 XbaI T'CTAG_A Cuts at: 3376 3376 Size: 6040 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2245 2245 Size: 6040 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6040 XmaI C'CCGG_G Cuts at: 1870 3365 1870 Size: 1495 4545 XmnI GAAnn'nnTTC Cuts at: 4169 4169 Size: 6040 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3848 276 Size: 53 83 186 936 2314 2468 Fragments arranged by size: 2468 2314 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI