(Circular) (Six-base) MAPSORT of: pFN21AE3635.seq Check: 4146 from: 1 to: 6091 pFN21AE3635 6091 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3028 3028 Size: 6091 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3357 3901 278 Size: 53 83 186 936 1821 544 2468 Fragments arranged by size: 2468 1821 936 544 186 83 53 AccI GT'mk_AC Cuts at: 1937 3434 1937 Size: 1497 4594 Acc65I G'GTAC_C Cuts at: 3412 3412 Size: 6091 AclI AA'CG_TT Cuts at: 1563 4218 4591 1563 Size: 2655 373 3063 Fragments arranged by size: 3063 2655 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2122 4154 5538 5806 1632 Size: 490 2032 1384 268 1917 Fragments arranged by size: 2032 1917 1384 490 268 AfeI AGC'GCT Cuts at: 3362 3362 Size: 6091 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5894 AflIII A'CryG_T Cuts at: 1203 2123 4990 5967 1203 Size: 920 2867 977 1327 Fragments arranged by size: 2867 1327 977 920 AgeI A'CCGG_T Cuts at: 4922 4922 Size: 6091 AhdI GACnn_n'nnGTC Cuts at: 4820 4820 Size: 6091 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6091 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2075 1184 Size: 891 5200 AlwNI CAG_nnn'CTG Cuts at: 2614 3241 3834 5406 2614 Size: 627 593 1572 3299 Fragments arranged by size: 3299 1572 627 593 ApaLI G'TGCA_C Cuts at: 4148 5304 4148 Size: 1156 4935 ApoI r'AATT_y Cuts at: 1493 2066 2237 3400 3626 3778 5887 1493 Size: 573 171 1163 226 152 2109 1697 Fragments arranged by size: 2109 1697 1163 573 226 171 152 AseI AT'TA_AT Cuts at: 160 1032 4645 160 Size: 872 3613 1606 Fragments arranged by size: 3613 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 AvaI C'yCGr_G Cuts at: 1870 1943 2430 3045 3416 1870 Size: 73 487 615 371 4545 Fragments arranged by size: 4545 615 487 371 73 BamHI G'GATC_C Cuts at: 3421 3788 3421 Size: 367 5724 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2112 3412 4867 618 Size: 325 217 627 325 1300 1455 1842 Fragments arranged by size: 1842 1455 1300 627 325 325 217 BanII G_rGCy'C Cuts at: 1391 2087 2148 2319 2501 2556 3121 3410 1391 Size: 696 61 171 182 55 565 289 4072 Fragments arranged by size: 4072 696 565 289 182 171 61 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2128 928 Size: 971 229 4891 Fragments arranged by size: 4891 971 229 BbvCI CC'TCA_GC Cuts at: 2540 2540 Size: 6091 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4282 4316 1903 Size: 34 2345 34 3678 Fragments arranged by size: 3678 2345 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3983 5193 3983 Size: 1210 4881 BclI T'GATC_A Cuts at: 1548 1548 Size: 6091 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4702 136 Size: 107 122 71 631 605 3030 1525 Fragments arranged by size: 3030 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6086 6086 Size: 6091 BlpI GC'TnA_GC Cuts at: 2273 2532 2634 2723 3508 2273 Size: 259 102 89 785 4856 Fragments arranged by size: 4856 785 259 102 89 Bme1580I G_kGCm'C Cuts at: 1790 2115 2903 3348 4152 5308 1790 Size: 325 788 445 804 1156 2573 Fragments arranged by size: 2573 1156 804 788 445 325 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6091 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 2499 3119 Size: 416 53 83 399 24 759 323 620 Cuts at: 3119 3822 4780 442 Size: 703 958 1753 Fragments arranged by size: 1753 958 759 703 620 416 399 323 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6091 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2722 2790 2853 2722 Size: 68 63 5960 Fragments arranged by size: 5960 68 63 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2303 2595 2910 4751 1239 Size: 971 93 292 315 1841 2579 Fragments arranged by size: 2579 1841 971 315 292 93 Bpu10I CC'TnA_GC Cuts at: 2081 2250 2540 2081 Size: 169 290 5632 Fragments arranged by size: 5632 290 169 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4216 5081 5379 4216 Size: 865 298 4928 Fragments arranged by size: 4928 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4754 882 Size: 617 3255 2219 Fragments arranged by size: 3255 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3882 493 Size: 711 2678 2702 Fragments arranged by size: 2702 2678 711 BsaBI GATnn'nnATC Cuts at: 6085 6085 Size: 6091 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3354 3898 4280 4915 Size: 53 83 186 936 1821 544 382 635 Cuts at: 4915 275 Size: 1451 Fragments arranged by size: 1821 1451 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2283 3207 4524 4922 5196 5343 5754 2283 Size: 924 1317 398 274 147 411 2620 Fragments arranged by size: 2620 1317 924 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2289 2319 2426 2456 1178 Size: 30 165 30 886 30 107 30 4813 Fragments arranged by size: 4813 886 165 107 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2477 2528 1186 Size: 1291 51 4749 Fragments arranged by size: 4749 1291 51 BseYI C'CCAG_C Cuts at: 2410 2549 2894 3087 3286 5294 5790 2410 Size: 139 345 193 199 2008 496 2711 Fragments arranged by size: 2711 2008 496 345 199 193 139 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2529 3077 1163 Size: 573 793 548 4177 Fragments arranged by size: 4177 793 573 548 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3342 3541 4302 5330 1540 Size: 275 186 1341 199 761 1028 2301 Fragments arranged by size: 2301 1341 1028 761 275 199 186 BsiHKAI G_wGCw'C Cuts at: 2015 2148 3297 4152 4237 5308 2015 Size: 133 1149 855 85 1071 2798 Fragments arranged by size: 2798 1149 1071 855 133 85 BsmI GAATG_Cn' Cuts at: 3602 3695 3602 Size: 93 5998 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2015 2087 2115 2148 2319 2501 2556 Size: 399 225 72 28 33 171 182 55 Cuts at: 2556 2903 3121 3297 3348 3410 4152 4237 5308 Size: 347 218 176 51 62 742 85 1071 Cuts at: 5308 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 347 225 218 182 176 171 85 72 62 55 51 33 28 BspEI T'CCGG_A Cuts at: 2283 5754 2283 Size: 3471 2620 BspHI T'CATG_A Cuts at: 2556 3980 5710 2556 Size: 1424 1730 2937 Fragments arranged by size: 2937 1730 1424 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3028 3446 844 Size: 2184 418 3489 Fragments arranged by size: 3489 2184 418 BsrBI CCG'CTC Cuts at: 1394 1823 2277 3978 1394 Size: 429 454 1701 3507 Fragments arranged by size: 3507 1701 454 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2361 2985 4586 4760 65 Size: 1183 1113 624 1601 174 1396 Fragments arranged by size: 1601 1396 1183 1113 624 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4735 4922 1700 Size: 110 2925 187 2869 Fragments arranged by size: 2925 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6091 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6091 BssSI C'ACGA_G Cuts at: 3296 4151 5163 3296 Size: 855 1012 4224 Fragments arranged by size: 4224 1012 855 BstAPI GCAn_nnn'nTGC Cuts at: 794 2200 2662 2797 794 Size: 1406 462 135 4088 Fragments arranged by size: 4088 1406 462 135 BstBI TT'CG_AA Cuts at: 4895 4895 Size: 6091 BstEII G'GTnAC_C Cuts at: 1184 3040 3331 1184 Size: 1856 291 3944 Fragments arranged by size: 3944 1856 291 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2860 3028 2128 Size: 732 168 5191 Fragments arranged by size: 5191 732 168 BstYI r'GATC_y Cuts at: 1972 2286 3174 3421 3788 4173 4190 5642 5728 Size: 314 888 247 367 385 17 1452 86 Cuts at: 5728 5740 6086 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 888 385 367 346 314 247 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3107 3107 Size: 6091 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2482 487 Size: 683 56 1256 4096 Fragments arranged by size: 4096 1256 683 56 BtsI GCAGTG_nn' Cuts at: 3607 4401 4421 3607 Size: 794 20 5277 Fragments arranged by size: 5277 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6091 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6056 DraI TTT'AAA Cuts at: 3395 3395 Size: 6091 DraIII CAC_nnn'GTG Cuts at: 3127 3127 Size: 6091 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5098 809 Size: 507 3782 1802 Fragments arranged by size: 3782 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2862 3312 3339 Size: 54 1425 50 275 18 1032 450 27 Cuts at: 3339 3538 4427 6056 8 Size: 199 889 1629 43 Fragments arranged by size: 1629 1425 1032 889 450 275 199 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 3339 3538 1537 Size: 275 1527 199 4090 Fragments arranged by size: 4090 1527 275 199 EarI CTCTTCn'nnn_ Cuts at: 1353 2555 2720 2822 2999 4021 1353 Size: 1202 165 102 177 1022 3423 Fragments arranged by size: 3423 1202 1022 177 165 102 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2336 4678 5052 5198 387 Size: 1064 138 747 2342 374 146 1280 Fragments arranged by size: 2342 1280 1064 747 374 146 138 EcoICRI GAG'CTC Cuts at: 2146 2146 Size: 6091 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2122 2210 2303 2595 2910 4154 4751 Size: 393 490 88 93 292 315 1244 597 Cuts at: 4751 5538 5806 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1244 787 597 490 393 315 292 268 93 88 EcoNI CCTnn'n_nnAGG Cuts at: 2543 2543 Size: 6091 EcoO109I rG'GnC_Cy Cuts at: 2077 2323 2077 Size: 246 5845 EcoRI G'AATT_C Cuts at: 2066 2237 3400 3778 2066 Size: 171 1163 378 4379 Fragments arranged by size: 4379 1163 378 171 FspI TGC'GCA Cuts at: 4597 4597 Size: 6091 HaeII r_GCGC'y Cuts at: 1678 3364 5238 1678 Size: 1686 1874 2531 Fragments arranged by size: 2531 1874 1686 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2135 2167 2426 2458 2995 Size: 32 135 32 563 32 259 32 537 Cuts at: 2995 3027 4732 4764 4806 4838 1373 Size: 32 1705 32 42 32 2626 Fragments arranged by size: 2626 1705 563 537 259 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3435 3681 669 Size: 988 281 1497 246 3079 Fragments arranged by size: 3079 1497 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6091 HpaI GTT'AAC Cuts at: 3681 3681 Size: 6091 KpnI G_GTAC'C Cuts at: 3416 3416 Size: 6091 MfeI C'AATT_G Cuts at: 3690 3690 Size: 6091 MluI A'CGCG_T Cuts at: 5967 5967 Size: 6091 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2946 5205 5389 1305 Size: 1641 2259 184 2007 Fragments arranged by size: 2259 2007 1641 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2864 10 Size: 54 1425 343 1032 3237 Fragments arranged by size: 3237 1425 1032 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2799 2927 3029 3329 4049 4408 4567 Size: 648 1633 128 102 300 720 359 159 Cuts at: 4567 518 Size: 2042 Fragments arranged by size: 2042 1633 720 648 359 300 159 128 102 MspA1I CmG'CkG Cuts at: 1606 2302 2410 2513 2600 3507 4184 5332 5577 Size: 696 108 103 87 907 677 1148 245 Cuts at: 5577 1606 Size: 2120 Fragments arranged by size: 2120 1148 907 696 677 245 108 103 87 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4600 Fragments arranged by size: 4600 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6091 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6091 NotI GC'GGCC_GC Cuts at: 3538 3538 Size: 6091 NspI r_CATG'y Cuts at: 1132 1229 2127 2429 3449 4994 1132 Size: 97 898 302 1020 1545 2229 Fragments arranged by size: 2229 1545 1020 898 302 97 PasI CC'CwG_GG Cuts at: 1348 2087 1348 Size: 739 5352 PciI A'CATG_T Cuts at: 2123 4990 2123 Size: 2867 3224 PflMI CCAn_nnn'nTGG Cuts at: 1108 2527 1108 Size: 1419 4672 PmeI GTTT'AAAC Cuts at: 3395 3395 Size: 6091 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4160 4192 5673 5705 4160 Size: 32 1481 32 4546 Fragments arranged by size: 4546 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2403 1613 Size: 790 5301 PsiI TTA'TAA Cuts at: 3661 3661 Size: 6091 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3221 3253 3221 Size: 32 6059 PstI C_TGCA'G Cuts at: 830 2064 2976 3443 830 Size: 1234 912 467 3478 Fragments arranged by size: 3478 1234 912 467 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6091 PvuII CAG'CTG Cuts at: 2302 2410 2513 2302 Size: 108 103 5880 Fragments arranged by size: 5880 108 103 SacI G_AGCT'C Cuts at: 2148 2148 Size: 6091 SalI G'TCGA_C Cuts at: 1936 3433 1936 Size: 1497 4594 SbfI CC_TGCA'GG Cuts at: 3443 3443 Size: 6091 ScaI AGT'ACT Cuts at: 2566 4339 6051 2566 Size: 1773 1712 2606 Fragments arranged by size: 2606 1773 1712 SexAI A'CCwGG_T Cuts at: 2691 2691 Size: 6091 SfcI C'TryA_G Cuts at: 826 1045 2060 2214 2972 3439 4574 5255 5446 Size: 219 1015 154 758 467 1135 681 191 Cuts at: 5446 826 Size: 1471 Fragments arranged by size: 1471 1135 1015 758 681 467 219 191 154 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6091 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6091 SmaI CCC'GGG Cuts at: 1872 2432 3047 3418 1872 Size: 560 615 371 4545 Fragments arranged by size: 4545 615 560 371 SmlI C'TyrA_G Cuts at: 820 1017 1943 4195 5096 5358 820 Size: 197 926 2252 901 262 1553 Fragments arranged by size: 2252 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3882 493 Size: 3389 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6091 SphI G_CATG'C Cuts at: 1132 2429 3449 1132 Size: 1297 1020 3774 Fragments arranged by size: 3774 1297 1020 SspI AAT'ATT Cuts at: 5 52 4015 5 Size: 47 3963 2081 Fragments arranged by size: 3963 2081 47 StuI AGG'CCT Cuts at: 1517 2387 1517 Size: 870 5221 StyI C'CwwG_G Cuts at: 513 1065 2004 2154 3530 513 Size: 552 939 150 1376 3074 Fragments arranged by size: 3074 1376 939 552 150 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2115 2158 2363 3138 4118 4135 4288 Size: 1255 535 43 205 775 980 17 153 Cuts at: 4288 4473 4918 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 980 775 535 445 205 185 153 43 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2038 2261 2564 Size: 274 80 33 51 1443 61 223 303 Cuts at: 2564 4337 6049 96 Size: 1773 1712 138 Fragments arranged by size: 1773 1712 1443 303 274 223 138 80 61 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2289 2321 6051 6083 2289 Size: 32 3730 32 2297 Fragments arranged by size: 3730 2297 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6091 XbaI T'CTAG_A Cuts at: 3427 3427 Size: 6091 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3028 3322 3028 Size: 294 5797 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6091 XmaI C'CCGG_G Cuts at: 1870 2430 3045 3416 1870 Size: 560 615 371 4545 Fragments arranged by size: 4545 615 560 371 XmnI GAAnn'nnTTC Cuts at: 2237 4220 2237 Size: 1983 4108 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3355 3899 276 Size: 53 83 186 936 1821 544 2468 Fragments arranged by size: 2468 1821 936 544 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI