################################## # NO CUT ENZYMES ( 126 enzymes) # ################################## AatII AccII AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BamHI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaBI BsaI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BstBI BstUI BstYI Bsu36I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HindIII HpaI KspI MaeI MamI MfeI MflI MluI MroI MseI MslI MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TfiI Tru9I Tsp509I Tth111I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 322 GGTACC G^GTACC AcyI 853 GGCGCC G[GA]^CG[CT]C Alw44I 460 GTGCAC G^TGCAC AlwI 784 GGATC GGATC(4/5) ApaI 828 GGGCCC GGGCC^C ApaLI 460 GTGCAC G^TGCAC Asp718I 322 GGTACC G^GTACC BalI 977 TGGCCA TGG^CCA BbeI 853 GGCGCC GGCGC^C BlpI 1012 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1012 GCTCAGC GC^T[ACGT]AGC BsaHI 853 GGCGCC G[GA]^CG[CT]C BsaWI 355 ACCGGA [AT]^CCGG[AT] BseRI 151 GAGGAG GAGGAG(10/8) BsmI 80 GAATGC GAATGC(1/-1) BspHI 602 TCATGA T^CATGA BssSI 1044 CTCGTG CACGAG(-5/-1) Bst1107I 766 GTATAC GTA^TAC BstEII 415 GGTCACC G^GT[ACGT]ACC BstPI 415 GGTCACC G^GT[ACGT]ACC BstZ17I 766 GTATAC GTA^TAC CelII 1012 GCTCAGC GC^T[ACGT]AGC DdeI 1013 CTCAG C^T[ACGT]AG DraIII 70 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 646 CTCTTC CTCTTC(1/4) EcoNI 422 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 415 GGTCACC G^GT[ACGT]ACC Hin1I 853 GGCGCC G[GA]^CG[CT]C HincII 310 GTCAAC GT[CT]^[GA]AC HindII 310 GTCAAC GT[CT]^[GA]AC HinfI 7 GACTC G^A[ACGT]TC KasI 853 GGCGCC G^GCGCC KpnI 322 GGTACC GGTAC^C Ksp632I 646 CTCTTC CTCTTC(1/4) MluNI 977 TGGCCA TGG^CCA MscI 977 TGGCCA TGG^CCA NarI 853 GGCGCC GG^CGCC PleI 7 GACTC GAGTC(4/5) PpuMI 138 GGGTCCC [GA]G^G[AT]CC[CT] PvuII 224 CAGCTG CAG^CTG RcaI 602 TCATGA T^CATGA SapI 645 GCTCTTC GCTCTTC(1/4) SexAI 35 ACCTGGT A^CC[AT]GGT SfiI 654 GGCCATCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 853 GGCGCC GGC^GCC SmaI 725 CCCGGG CCC^GGG TaqI 338 TCGA T^CGA TthHB8I 338 TCGA T^CGA XmaCI 725 CCCGGG C^CCGGG XmaI 725 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AflIII 272 ACGTGT A^C[GA][CT]GT 556 ACGTGT AvaI 106 CTCGGG C^[CT]CG[GA]G 725 CCCGGG BbrPI 70 CACGTG CAC^GTG 712 CACGTG BbsI 159 GAAGAC GAAGAC(2/6) 439 GAAGAC BpuAI 159 GAAGAC GAAGAC(2/6) 439 GAAGAC BsaAI 70 CACGTG [CT]AC^GT[GA] 712 CACGTG BsoBI 106 CTCGGG C^[CT]CG[GA]G 725 CCCGGG BsrDI 863 CATTGC GCAATG(2/0) 695 GCAATG BstAPI 69 GCACGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 915 GCAGTGGCTGC BstXI 252 CCACACGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 380 CCAGCAGCATGG HaeII 853 GGCGCC [GA]GCGC^[CT] 1016 AGCGCC PmaCI 70 CACGTG CAC^GTG 712 CACGTG PmlI 70 CACGTG CAC^GTG 712 CACGTG PstI 88 CTGCAG CTGCA^G 913 CTGCAG SfaNI 708 GATGC GCATC(5/9) 578 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AccI AciI AfaI AluI AlwNI AspHI AvaII BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfcI TseI Tsp45I TspRI Van91I