(Circular) (Six-base) MAPSORT of: pFN21AE6345.seq Check: 1643 from: 1 to: 5812 pFN21AE6345 5812 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3622 278 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2274 2379 2772 3155 1937 Size: 337 105 393 383 4594 Fragments arranged by size: 4594 393 383 337 105 Acc65I G'GTAC_C Cuts at: 2327 3133 2327 Size: 806 5006 AclI AA'CG_TT Cuts at: 1563 3939 4312 1563 Size: 2376 373 3063 Fragments arranged by size: 3063 2376 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2183 3875 5259 5527 1632 Size: 551 1692 1384 268 1917 Fragments arranged by size: 1917 1692 1384 551 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5615 AflIII A'CryG_T Cuts at: 1203 2277 2561 4711 5688 1203 Size: 1074 284 2150 977 1327 Fragments arranged by size: 2150 1327 1074 977 284 AgeI A'CCGG_T Cuts at: 4643 4643 Size: 5812 AhdI GACnn_n'nnGTC Cuts at: 4541 4541 Size: 5812 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5812 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5812 AlwNI CAG_nnn'CTG Cuts at: 2032 2174 2231 2632 2926 3555 5127 2032 Size: 142 57 401 294 629 1572 2717 Fragments arranged by size: 2717 1572 629 401 294 142 57 ApaI G_GGCC'C Cuts at: 2837 2837 Size: 5812 ApaLI G'TGCA_C Cuts at: 2465 3869 5025 2465 Size: 1404 1156 3252 Fragments arranged by size: 3252 1404 1156 ApoI r'AATT_y Cuts at: 1493 3121 3347 3499 5608 1493 Size: 1628 226 152 2109 1697 Fragments arranged by size: 2109 1697 1628 226 152 AseI AT'TA_AT Cuts at: 160 1032 4366 160 Size: 872 3334 1606 Fragments arranged by size: 3334 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 AvaI C'yCGr_G Cuts at: 1870 1943 2111 2730 3137 1870 Size: 73 168 619 407 4545 Fragments arranged by size: 4545 619 407 168 73 BamHI G'GATC_C Cuts at: 3142 3509 3142 Size: 367 5445 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2327 2472 2858 3133 4588 Size: 325 217 627 540 145 386 275 1455 Cuts at: 4588 618 Size: 1842 Fragments arranged by size: 1842 1455 627 540 386 325 275 217 145 BanII G_rGCy'C Cuts at: 1391 2106 2245 2837 3042 3131 1391 Size: 715 139 592 205 89 4072 Fragments arranged by size: 4072 715 592 205 139 89 BbeI G_GCGC'C Cuts at: 2862 2862 Size: 5812 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2171 2451 928 Size: 971 272 280 4289 Fragments arranged by size: 4289 971 280 272 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4003 4037 1903 Size: 34 2066 34 3678 Fragments arranged by size: 3678 2066 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3704 4914 3704 Size: 1210 4602 BclI T'GATC_A Cuts at: 1548 1548 Size: 5812 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2666 2881 3030 Size: 107 122 71 631 605 994 215 149 Cuts at: 3030 4423 136 Size: 1393 1525 Fragments arranged by size: 1525 1393 994 631 605 215 149 122 107 71 BglII A'GATC_T Cuts at: 5807 5807 Size: 5812 BlpI GC'TnA_GC Cuts at: 3018 3229 3018 Size: 211 5601 Bme1580I G_kGCm'C Cuts at: 1790 2469 2569 2837 3873 5029 1790 Size: 679 100 268 1036 1156 2573 Fragments arranged by size: 2573 1156 1036 679 268 100 BmgBI CAC'GTC Cuts at: 1318 2203 2278 2562 1318 Size: 885 75 284 4568 Fragments arranged by size: 4568 885 284 75 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3543 4501 442 Size: 416 53 83 399 24 2126 958 1753 Fragments arranged by size: 2126 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5812 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2666 2666 Size: 5812 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2537 2759 4472 1239 Size: 935 363 222 1713 2579 Fragments arranged by size: 2579 1713 935 363 222 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3937 4802 5100 3937 Size: 865 298 4649 Fragments arranged by size: 4649 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4475 882 Size: 617 2976 2219 Fragments arranged by size: 2976 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2077 2719 3603 493 Size: 711 873 642 884 2702 Fragments arranged by size: 2702 884 873 711 642 BsaBI GATnn'nnATC Cuts at: 5806 5806 Size: 5812 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2859 3619 4001 4636 Size: 53 83 186 936 1326 760 382 635 Cuts at: 4636 275 Size: 1451 Fragments arranged by size: 1451 1326 936 760 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2360 4245 4643 4917 5064 5475 2360 Size: 1885 398 274 147 411 2697 Fragments arranged by size: 2697 1885 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2061 2091 2232 2262 1178 Size: 30 165 30 658 30 141 30 4728 Fragments arranged by size: 4728 658 165 141 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 1186 Size: 985 4827 BseYI C'CCAG_C Cuts at: 2264 5015 5511 2264 Size: 2751 496 2565 Fragments arranged by size: 2751 2565 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2001 2342 2375 1163 Size: 573 265 341 33 4600 Fragments arranged by size: 4600 573 341 265 33 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3262 4023 5051 1540 Size: 275 186 1261 761 1028 2301 Fragments arranged by size: 2301 1261 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2469 2946 3873 3958 5029 2076 Size: 393 477 927 85 1071 2859 Fragments arranged by size: 2859 1071 927 477 393 85 BsmI GAATG_Cn' Cuts at: 2091 3323 3416 2091 Size: 1232 93 4487 Fragments arranged by size: 4487 1232 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2106 2245 2469 2569 2837 2946 Size: 399 286 30 139 224 100 268 109 Cuts at: 2946 3042 3131 3873 3958 5029 1391 Size: 96 89 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 286 268 224 139 109 100 96 89 85 30 BspEI T'CCGG_A Cuts at: 5475 5475 Size: 5812 BspHI T'CATG_A Cuts at: 2607 3701 5431 2607 Size: 1094 1730 2988 Fragments arranged by size: 2988 1730 1094 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3167 844 Size: 2323 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3699 1394 Size: 429 1876 3507 Fragments arranged by size: 3507 1876 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2707 2867 4307 4481 65 Size: 1183 1459 160 1440 174 1396 Fragments arranged by size: 1459 1440 1396 1183 174 160 BsrFI r'CCGG_y Cuts at: 1700 1810 4456 4643 1700 Size: 110 2646 187 2869 Fragments arranged by size: 2869 2646 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5812 BssSI C'ACGA_G Cuts at: 3049 3872 4884 3049 Size: 823 1012 3977 Fragments arranged by size: 3977 1012 823 BstAPI GCAn_nnn'nTGC Cuts at: 794 2080 2926 794 Size: 1286 846 3680 Fragments arranged by size: 3680 1286 846 BstBI TT'CG_AA Cuts at: 4616 4616 Size: 5812 BstEII G'GTnAC_C Cuts at: 1184 2420 1184 Size: 1236 4576 BstXI CCAn_nnnn'nTGG Cuts at: 2264 2392 2264 Size: 128 5684 BstYI r'GATC_y Cuts at: 1972 3142 3509 3894 3911 5363 5449 5461 5807 Size: 1170 367 385 17 1452 86 12 346 Cuts at: 5807 1972 Size: 1977 Fragments arranged by size: 1977 1452 1170 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2773 2773 Size: 5812 BtgI C'CryG_G Cuts at: 513 1065 2004 2539 2577 2979 513 Size: 552 939 535 38 402 3346 Fragments arranged by size: 3346 939 552 535 402 38 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5073 Fragments arranged by size: 5073 683 56 BtsI GCAGTG_nn' Cuts at: 2469 2927 3328 4122 4142 2469 Size: 458 401 794 20 4139 Fragments arranged by size: 4139 794 458 401 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5812 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5777 DraI TTT'AAA Cuts at: 3116 3116 Size: 5812 DraIII CAC_nnn'GTG Cuts at: 2080 2080 Size: 5812 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4819 809 Size: 507 3503 1802 Fragments arranged by size: 3503 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2235 2499 Size: 54 1425 50 275 18 177 228 264 Cuts at: 2499 2683 2764 2933 2976 2982 3259 4148 5777 Size: 184 81 169 43 6 277 889 1629 Cuts at: 5777 8 Size: 43 Fragments arranged by size: 1629 1425 889 277 275 264 228 184 177 169 81 54 50 43 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3259 1537 Size: 275 1447 4090 Fragments arranged by size: 4090 1447 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2657 3742 1353 Size: 1304 1085 3423 Fragments arranged by size: 3423 1304 1085 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4399 4773 4919 387 Size: 1064 138 2810 374 146 1280 Fragments arranged by size: 2810 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2183 2537 2759 3875 4472 5259 Size: 393 542 9 354 222 1116 597 787 Cuts at: 5259 5527 1239 Size: 268 1524 Fragments arranged by size: 1524 1116 787 597 542 393 354 268 222 9 EcoNI CCTnn'n_nnAGG Cuts at: 2431 2431 Size: 5812 EcoO109I rG'GnC_Cy Cuts at: 2144 2171 2405 2494 2629 2834 2144 Size: 27 234 89 135 205 5122 Fragments arranged by size: 5122 234 205 135 89 27 EcoRI G'AATT_C Cuts at: 3121 3499 3121 Size: 378 5434 FspI TGC'GCA Cuts at: 4318 4318 Size: 5812 HaeII r_GCGC'y Cuts at: 1678 2862 3025 4959 1678 Size: 1184 163 1934 2531 Fragments arranged by size: 2531 1934 1184 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4453 4485 4527 4559 1373 Size: 32 135 32 2881 32 42 32 2626 Fragments arranged by size: 2881 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2317 3156 3402 669 Size: 988 281 379 839 246 3079 Fragments arranged by size: 3079 988 839 379 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5812 HpaI GTT'AAC Cuts at: 3402 3402 Size: 5812 KasI G'GCGC_C Cuts at: 2858 2858 Size: 5812 KpnI G_GTAC'C Cuts at: 2331 3137 2331 Size: 806 5006 MfeI C'AATT_G Cuts at: 3411 3411 Size: 5812 MluI A'CGCG_T Cuts at: 5688 5688 Size: 5812 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4926 5110 1305 Size: 3621 184 2007 Fragments arranged by size: 3621 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2984 10 Size: 54 1425 343 1152 2838 Fragments arranged by size: 2838 1425 1152 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3770 4129 4288 518 Size: 648 2604 359 159 2042 Fragments arranged by size: 2604 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2231 2746 3228 3905 5053 5298 1606 Size: 492 133 515 482 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 515 492 482 245 133 NarI GG'CG_CC Cuts at: 2859 2859 Size: 5812 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4321 Fragments arranged by size: 4321 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5812 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5812 NotI GC'GGCC_GC Cuts at: 3259 3259 Size: 5812 NspI r_CATG'y Cuts at: 1132 1229 3170 4715 1132 Size: 97 1941 1545 2229 Fragments arranged by size: 2229 1941 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5812 PciI A'CATG_T Cuts at: 4711 4711 Size: 5812 PflMI CCAn_nnn'nTGG Cuts at: 1108 2032 2140 2221 3081 1108 Size: 924 108 81 860 3839 Fragments arranged by size: 3839 924 860 108 81 PfoI T'CCnGG_A Cuts at: 2514 2514 Size: 5812 PmeI GTTT'AAAC Cuts at: 3116 3116 Size: 5812 PmlI CAC'GTG Cuts at: 2077 2719 2077 Size: 642 5170 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3029 3061 3881 3913 5394 5426 3029 Size: 32 820 32 1481 32 3415 Fragments arranged by size: 3415 1481 820 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2144 2144 Size: 5812 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5812 PsiI TTA'TAA Cuts at: 3382 3382 Size: 5812 PspOMI G'GGCC_C Cuts at: 2833 2833 Size: 5812 PstI C_TGCA'G Cuts at: 830 2097 2922 3164 830 Size: 1267 825 242 3478 Fragments arranged by size: 3478 1267 825 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5812 PvuII CAG'CTG Cuts at: 2231 2231 Size: 5812 SalI G'TCGA_C Cuts at: 1936 3154 1936 Size: 1218 4594 SanDI GG'GwC_CC Cuts at: 2144 2144 Size: 5812 SapI GCTCTTCn'nnn_ Cuts at: 2657 2657 Size: 5812 SbfI CC_TGCA'GG Cuts at: 3164 3164 Size: 5812 ScaI AGT'ACT Cuts at: 4060 5772 4060 Size: 1712 4100 SexAI A'CCwGG_T Cuts at: 2040 2040 Size: 5812 SfcI C'TryA_G Cuts at: 826 1045 2093 2431 2797 2918 3160 4295 4976 Size: 219 1048 338 366 121 242 1135 681 Cuts at: 4976 5167 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1048 681 366 338 242 219 191 121 SfiI GGCCn_nnn'nGGCC Cuts at: 2666 2666 Size: 5812 SfoI GGC'GCC Cuts at: 2860 2860 Size: 5812 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5812 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5812 SmaI CCC'GGG Cuts at: 1872 2732 3139 1872 Size: 860 407 4545 Fragments arranged by size: 4545 860 407 SmlI C'TyrA_G Cuts at: 820 1017 1943 3916 4817 5079 820 Size: 197 926 1973 901 262 1553 Fragments arranged by size: 1973 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3603 493 Size: 3110 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5812 SphI G_CATG'C Cuts at: 1132 3170 1132 Size: 2038 3774 SspI AAT'ATT Cuts at: 5 52 3736 5 Size: 47 3684 2081 Fragments arranged by size: 3684 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5812 StyI C'CwwG_G Cuts at: 513 1065 2004 3251 513 Size: 552 939 1247 3074 Fragments arranged by size: 3074 1247 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3839 3856 4009 4194 4639 325 Size: 1255 2259 17 153 185 445 1498 Fragments arranged by size: 2259 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4058 5770 96 Size: 274 80 33 51 1443 2081 1712 138 Fragments arranged by size: 2081 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2061 2093 2232 2264 2524 2556 5772 5804 2061 Size: 32 139 32 260 32 3216 32 2069 Fragments arranged by size: 3216 2069 260 139 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5812 XbaI T'CTAG_A Cuts at: 3148 3148 Size: 5812 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5812 XmaI C'CCGG_G Cuts at: 1870 2730 3137 1870 Size: 860 407 4545 Fragments arranged by size: 4545 860 407 XmnI GAAnn'nnTTC Cuts at: 3941 3941 Size: 5812 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3620 276 Size: 53 83 186 936 2086 2468 Fragments arranged by size: 2468 2086 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI Bsu36I EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SrfI SwaI XcmI