(Linear) (Six-base) MAPSORT of: KIEE6345.seq Check: 5794 from: 1 to: 1107 KIEE6345.orf With 182 enzymes: SgfI * March 16, 2015 11:30 .. AccI GT'mk_AC Cuts at: 0 269 374 767 1107 Size: 269 105 393 340 Fragments arranged by size: 393 340 269 105 Acc65I G'GTAC_C Cuts at: 0 322 1107 Size: 322 785 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 178 1107 Size: 178 929 AflIII A'CryG_T Cuts at: 0 272 556 1107 Size: 272 284 551 Fragments arranged by size: 551 284 272 AlwNI CAG_nnn'CTG Cuts at: 0 27 169 226 627 921 1107 Size: 27 142 57 401 294 186 Fragments arranged by size: 401 294 186 142 57 27 ApaI G_GGCC'C Cuts at: 0 832 1107 Size: 832 275 ApaLI G'TGCA_C Cuts at: 0 460 1107 Size: 460 647 AvaI C'yCGr_G Cuts at: 0 106 725 1107 Size: 106 619 382 Fragments arranged by size: 619 382 106 BanI G'GyrC_C Cuts at: 0 322 467 853 1107 Size: 322 145 386 254 Fragments arranged by size: 386 322 254 145 BanII G_rGCy'C Cuts at: 0 101 240 832 1037 1107 Size: 101 139 592 205 70 Fragments arranged by size: 592 205 139 101 70 BbeI G_GCGC'C Cuts at: 0 857 1107 Size: 857 250 BbsI GAAGACnn'nnnn_ Cuts at: 0 166 446 1107 Size: 166 280 661 Fragments arranged by size: 661 280 166 BglI GCCn_nnn'nGGC Cuts at: 0 661 876 1025 1107 Size: 661 215 149 82 Fragments arranged by size: 661 215 149 82 BlpI GC'TnA_GC Cuts at: 0 1013 1107 Size: 1013 94 Bme1580I G_kGCm'C Cuts at: 0 464 564 832 1107 Size: 464 100 268 275 Fragments arranged by size: 464 275 268 100 BmgBI CAC'GTC Cuts at: 0 198 273 557 1107 Size: 198 75 284 550 Fragments arranged by size: 550 284 198 75 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 661 1107 Size: 661 446 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 532 754 1107 Size: 169 363 222 353 Fragments arranged by size: 363 353 222 169 BsaAI yAC'GTr Cuts at: 0 72 714 1107 Size: 72 642 393 Fragments arranged by size: 642 393 72 BsaHI Gr'CG_yC Cuts at: 0 854 1107 Size: 854 253 BsaWI w'CCGG_w Cuts at: 0 355 1107 Size: 355 752 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 56 86 227 257 1107 Size: 56 30 141 30 850 Fragments arranged by size: 850 141 56 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 166 1107 Size: 166 941 BseYI C'CCAG_C Cuts at: 0 259 1107 Size: 259 848 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 370 1107 Size: 337 33 737 Fragments arranged by size: 737 337 33 BsiHKAI G_wGCw'C Cuts at: 0 71 464 941 1107 Size: 71 393 477 166 Fragments arranged by size: 477 393 166 71 BsmI GAATG_Cn' Cuts at: 0 86 1107 Size: 86 1021 Bsp1286I G_dGCh'C Cuts at: 0 71 101 240 464 564 832 941 1037 Size: 71 30 139 224 100 268 109 96 Cuts at: 1037 1107 Size: 70 Fragments arranged by size: 268 224 139 109 100 96 71 70 30 BspHI T'CATG_A Cuts at: 0 602 1107 Size: 602 505 BsrDI GCAATG_nn' Cuts at: 0 702 862 1107 Size: 702 160 245 Fragments arranged by size: 702 245 160 BssSI C'ACGA_G Cuts at: 0 1044 1107 Size: 1044 63 BstAPI GCAn_nnn'nTGC Cuts at: 0 75 921 1107 Size: 75 846 186 Fragments arranged by size: 846 186 75 BstEII G'GTnAC_C Cuts at: 0 415 1107 Size: 415 692 BstXI CCAn_nnnn'nTGG Cuts at: 0 259 387 1107 Size: 259 128 720 Fragments arranged by size: 720 259 128 BstZ17I GTA'TAC Cuts at: 0 768 1107 Size: 768 339 BtgI C'CryG_G Cuts at: 0 534 572 974 1107 Size: 534 38 402 133 Fragments arranged by size: 534 402 133 38 BtsI GCAGTG_nn' Cuts at: 0 464 922 1107 Size: 464 458 185 Fragments arranged by size: 464 458 185 DraIII CAC_nnn'GTG Cuts at: 0 75 1107 Size: 75 1032 EaeI y'GGCC_r Cuts at: 0 2 230 494 678 759 928 971 977 Size: 2 228 264 184 81 169 43 6 Cuts at: 977 1107 Size: 130 Fragments arranged by size: 264 228 184 169 130 81 43 6 2 EarI CTCTTCn'nnn_ Cuts at: 0 652 1107 Size: 652 455 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 169 178 532 754 1107 Size: 169 9 354 222 353 Fragments arranged by size: 354 353 222 169 9 EcoNI CCTnn'n_nnAGG Cuts at: 0 426 1107 Size: 426 681 EcoO109I rG'GnC_Cy Cuts at: 0 139 166 400 489 624 829 1107 Size: 139 27 234 89 135 205 278 Fragments arranged by size: 278 234 205 139 135 89 27 HaeII r_GCGC'y Cuts at: 0 857 1020 1107 Size: 857 163 87 Fragments arranged by size: 857 163 87 HincII GTy'rAC Cuts at: 0 312 1107 Size: 312 795 KasI G'GCGC_C Cuts at: 0 853 1107 Size: 853 254 KpnI G_GTAC'C Cuts at: 0 326 1107 Size: 326 781 MscI TGG'CCA Cuts at: 0 979 1107 Size: 979 128 MspA1I CmG'CkG Cuts at: 0 93 226 741 1107 Size: 93 133 515 366 Fragments arranged by size: 515 366 133 93 NarI GG'CG_CC Cuts at: 0 854 1107 Size: 854 253 PflMI CCAn_nnn'nTGG Cuts at: 0 27 135 216 1076 1107 Size: 27 108 81 860 31 Fragments arranged by size: 860 108 81 31 27 PfoI T'CCnGG_A Cuts at: 0 509 1107 Size: 509 598 PmlI CAC'GTG Cuts at: 0 72 714 1107 Size: 72 642 393 Fragments arranged by size: 642 393 72 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1024 1056 1107 Size: 1024 32 51 Fragments arranged by size: 1024 51 32 PpuMI rG'GwC_Cy Cuts at: 0 139 1107 Size: 139 968 PspOMI G'GGCC_C Cuts at: 0 828 1107 Size: 828 279 PstI C_TGCA'G Cuts at: 0 92 917 1107 Size: 92 825 190 Fragments arranged by size: 825 190 92 PvuII CAG'CTG Cuts at: 0 226 1107 Size: 226 881 SanDI GG'GwC_CC Cuts at: 0 139 1107 Size: 139 968 SapI GCTCTTCn'nnn_ Cuts at: 0 652 1107 Size: 652 455 SexAI A'CCwGG_T Cuts at: 0 35 1107 Size: 35 1072 SfcI C'TryA_G Cuts at: 0 88 426 792 913 1107 Size: 88 338 366 121 194 Fragments arranged by size: 366 338 194 121 88 SfiI GGCCn_nnn'nGGCC Cuts at: 0 661 1107 Size: 661 446 SfoI GGC'GCC Cuts at: 0 855 1107 Size: 855 252 SmaI CCC'GGG Cuts at: 0 727 1107 Size: 727 380 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 56 88 227 259 519 551 1107 Size: 56 32 139 32 260 32 556 Fragments arranged by size: 556 260 139 56 32 32 32 XmaI C'CCGG_G Cuts at: 0 725 1107 Size: 725 382 Enzymes that do cut: AccI Acc65I AcuI AflIII AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI BglI BlpI Bme1580I BmgBI BplI BpmI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BssSI BstAPI BstEII BstXI BstZ17I BtgI BtsI DraIII EaeI EarI Eco57MI EcoNI EcoO109I HaeII HincII KasI KpnI MscI MspA1I NarI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SanDI SapI SexAI SfcI SfiI SfoI SmaI TstI XmaI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI BsaBI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BstBI BstYI Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SbfI ScaI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI