################################## # NO CUT ENZYMES ( 173 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT14I FbaI FokI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MluI MluNI MroI MscI MslI MspI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccII 139 CGCG CG^CG AcsI 595 AAATTC [GA]^AATT[CT] ApoI 595 AAATTC [GA]^AATT[CT] Asp700I 627 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 68 GAGCAC G[AT]GC[AT]^C BbsI 511 GAAGAC GAAGAC(2/6) BglII 399 AGATCT A^GATCT BpmI 172 CTGGAG CTGGAG(16/14) BpuAI 511 GAAGAC GAAGAC(2/6) BsaJI 80 CCGAGG C^C[ACGT][ACGT]GG BsgI 339 GTGCAG GTGCAG(16/14) BsiHKAI 68 GAGCAC G[AT]GC[AT]^C BsmAI 27 GTCTC GTCTC(1/5) BsmFI 332 GGGAC GGGAC(10/14) BstUI 139 CGCG CG^CG BstYI 399 AGATCT [GA]^GATC[CT] DdeI 29 CTCAG C^T[ACGT]AG DrdI 46 GACTCCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 3 GAAGAG CTCTTC(1/4) EcoT22I 20 ATGCAT ATGCA^T Ksp632I 3 GAAGAG CTCTTC(1/4) MaeIII 424 GTGAC ^GT[ACGT]AC MfeI 650 CAATTG C^AATTG MflI 399 AGATCT [GA]^GATC[CT] MunI 650 CAATTG C^AATTG MvnI 139 CGCG CG^CG NlaIV 332 GGGACC GG[ACGT]^[ACGT]CC NsiI 20 ATGCAT ATGCA^T PleI 46 GACTC GAGTC(4/5) PpuMI 332 GGGACCT [GA]G^G[AT]CC[CT] PstI 151 CTGCAG CTGCA^G PvuII 148 CAGCTG CAG^CTG ScaI 176 AGTACT AGT^ACT SmlI 349 CTCAAG C^T[CT][GA]AG Tsp45I 424 GTGAC ^GT[GC]AC XhoII 399 AGATCT [GA]^GATC[CT] XmnI 627 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 177 GTAC GT^AC 324 GTAC BanII 126 GAGCCC G[GA]GC[CT]^C 346 GGGCTC BssKI 171 CCTGG ^CC[ACGT]GG 233 CCAGG BstNI 171 CCTGG CC^[AT]GG 233 CCAGG DpnII 12 GATC ^GATC 400 GATC DraII 94 AGGGCCT [GA]G^G[ACGT]CC[CT] 332 GGGACCT EcoNI 86 CCTTCGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 468 CCTTCTACAGG EcoO109I 94 AGGGCCT [GA]G^G[ACGT]CC[CT] 332 GGGACCT EcoRII 171 CCTGG ^CC[AT]GG 233 CCAGG HphI 182 TCACC GGTGA(8/7) 503 TCACC MboI 12 GATC ^GATC 400 GATC MseI 266 TTAA T^TAA 391 TTAA MspA1I 73 CAGCGG C[AC]G^C[GT]G 148 CAGCTG MvaI 171 CCTGG CC^[AT]GG 233 CCAGG NdeII 12 GATC ^GATC 400 GATC RsaI 177 GTAC GT^AC 324 GTAC Sau3AI 12 GATC ^GATC 400 GATC ScrFI 171 CCTGG CC^[ACGT]GG 233 CCAGG SfcI 151 CTGCAG C^T[GA][CT]AG 472 CTACAG StuI 83 AGGCCT AGG^CCT 222 AGGCCT TfiI 375 GATTC G^A[AT]TC 464 GAATC Tru9I 266 TTAA T^TAA 391 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AluI AvaII BbvI BmyI BsiYI BslI Bsp1286I Cac8I CfoI Cfr13I FauI Fnu4HI HaeIII HhaI HinP1I HinfI ItaI MboII MnlI MwoI NlaIII Sau96I TseI Tsp509I TspRI