(Linear) (Six-base) MAPSORT of: KIEE5203.seq Check: 4001 from: 1 to: 660 KIEE5203.orf With 182 enzymes: SgfI * January 29, 2014 11:50 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 47 124 660 Size: 47 77 536 Fragments arranged by size: 536 77 47 ApoI r'AATT_y Cuts at: 0 595 660 Size: 595 65 BanII G_rGCy'C Cuts at: 0 130 350 660 Size: 130 220 310 Fragments arranged by size: 310 220 130 BbsI GAAGACnn'nnnn_ Cuts at: 0 518 660 Size: 518 142 BfrBI ATG'CAT Cuts at: 0 22 660 Size: 22 638 BglII A'GATC_T Cuts at: 0 399 660 Size: 399 261 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 660 Size: 193 467 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 334 660 Size: 334 326 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 660 Size: 360 300 BsiHKAI G_wGCw'C Cuts at: 0 72 660 Size: 72 588 Bsp1286I G_dGCh'C Cuts at: 0 72 130 350 660 Size: 72 58 220 310 Fragments arranged by size: 310 220 72 58 BstYI r'GATC_y Cuts at: 0 399 660 Size: 399 261 BtsI GCAGTG_nn' Cuts at: 0 348 660 Size: 348 312 DrdI GACnn_nn'nnGTC Cuts at: 0 52 660 Size: 52 608 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 47 124 193 660 Size: 47 77 69 467 Fragments arranged by size: 467 77 69 47 EcoNI CCTnn'n_nnAGG Cuts at: 0 90 472 660 Size: 90 382 188 Fragments arranged by size: 382 188 90 EcoO109I rG'GnC_Cy Cuts at: 0 95 333 660 Size: 95 238 327 Fragments arranged by size: 327 238 95 MfeI C'AATT_G Cuts at: 0 650 660 Size: 650 10 MspA1I CmG'CkG Cuts at: 0 75 150 660 Size: 75 75 510 Fragments arranged by size: 510 75 75 NsiI A_TGCA'T Cuts at: 0 24 660 Size: 24 636 PfoI T'CCnGG_A Cuts at: 0 170 660 Size: 170 490 PpuMI rG'GwC_Cy Cuts at: 0 333 660 Size: 333 327 PstI C_TGCA'G Cuts at: 0 155 660 Size: 155 505 PvuII CAG'CTG Cuts at: 0 150 660 Size: 150 510 ScaI AGT'ACT Cuts at: 0 178 660 Size: 178 482 SfcI C'TryA_G Cuts at: 0 151 472 660 Size: 151 321 188 Fragments arranged by size: 321 188 151 SmlI C'TyrA_G Cuts at: 0 349 660 Size: 349 311 StuI AGG'CCT Cuts at: 0 85 224 660 Size: 85 139 436 Fragments arranged by size: 436 139 85 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 596 660 Size: 596 64 TatI w'GTAC_w Cuts at: 0 176 323 660 Size: 176 147 337 Fragments arranged by size: 337 176 147 XmnI GAAnn'nnTTC Cuts at: 0 631 660 Size: 631 29 Enzymes that do cut: AcuI ApoI BanII BbsI BfrBI BglII BpmI BpuEI BsgI BsiHKAI Bsp1286I BstYI BtsI DrdI Eco57MI EcoNI EcoO109I MfeI MspA1I NsiI PfoI PpuMI PstI PvuII ScaI SfcI SmlI StuI TaqII TatI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII EaeI EagI EarI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MluI MmeI MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI TstI Tth111I XbaI XcmI XhoI XmaI ZraI