(Circular) (Six-base) MAPSORT of: pFN21AE6441.seq Check: 2055 from: 1 to: 6097 pFN21AE6441 6097 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2646 3440 1937 Size: 709 794 4594 Fragments arranged by size: 4594 794 709 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2261 2315 4160 5544 5812 1632 Size: 629 54 1845 1384 268 1917 Fragments arranged by size: 1917 1845 1384 629 268 54 AfeI AGC'GCT Cuts at: 2078 2078 Size: 6097 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 2816 3105 3147 4996 5973 1203 Size: 1613 289 42 1849 977 1327 Fragments arranged by size: 1849 1613 1327 977 289 42 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6097 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6097 AlwNI CAG_nnn'CTG Cuts at: 2753 3840 5412 2753 Size: 1087 1572 3438 Fragments arranged by size: 3438 1572 1087 ApaI G_GGCC'C Cuts at: 2298 2743 2298 Size: 445 5652 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2250 3406 3632 3784 5893 1493 Size: 757 1156 226 152 2109 1697 Fragments arranged by size: 2109 1697 1156 757 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2298 3422 1870 Size: 73 355 1124 4545 Fragments arranged by size: 4545 1124 355 73 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2082 2375 2562 3418 4873 Size: 325 217 627 295 293 187 856 1455 Cuts at: 4873 618 Size: 1842 Fragments arranged by size: 1842 1455 856 627 325 295 293 217 187 BanII G_rGCy'C Cuts at: 1391 2235 2298 2743 3416 1391 Size: 844 63 445 673 4072 Fragments arranged by size: 4072 844 673 445 63 BbeI G_GCGC'C Cuts at: 2086 2566 2086 Size: 480 5617 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2613 2880 928 Size: 971 714 267 4145 Fragments arranged by size: 4145 971 714 267 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3989 5199 3989 Size: 1210 4887 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4708 136 Size: 107 122 71 631 605 337 2699 1525 Fragments arranged by size: 2699 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3258 3514 3258 Size: 256 5841 Bme1580I G_kGCm'C Cuts at: 1790 2298 2743 4158 5314 1790 Size: 508 445 1415 1156 2573 Fragments arranged by size: 2573 1415 1156 508 445 BmgBI CAC'GTC Cuts at: 1318 2131 3108 1318 Size: 813 977 4307 Fragments arranged by size: 4307 977 813 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2756 3828 4786 Size: 416 53 83 399 24 1339 1072 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1339 1072 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2375 3068 3097 4757 1239 Size: 1136 693 29 1660 2579 Fragments arranged by size: 2579 1660 1136 693 29 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4760 882 Size: 617 3261 2219 Fragments arranged by size: 3261 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3888 493 Size: 711 2684 2702 Fragments arranged by size: 2702 2684 711 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2083 2563 3904 4286 Size: 53 83 186 936 550 480 1341 382 Cuts at: 4286 4921 275 Size: 635 1451 Fragments arranged by size: 1451 1341 936 635 550 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3315 4530 4928 5202 5349 5760 3315 Size: 1215 398 274 147 411 3652 Fragments arranged by size: 3652 1215 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2307 2337 3095 3125 1178 Size: 30 165 30 904 30 758 30 4150 Fragments arranged by size: 4150 904 758 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6097 BseYI C'CCAG_C Cuts at: 2062 2122 2713 2798 3360 5300 5796 2062 Size: 60 591 85 562 1940 496 2363 Fragments arranged by size: 2363 1940 591 562 496 85 60 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3019 3086 3386 1163 Size: 573 1283 67 300 3874 Fragments arranged by size: 3874 1283 573 300 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2777 3547 4308 5336 1540 Size: 275 186 776 770 761 1028 2301 Fragments arranged by size: 2301 1028 776 770 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2235 3378 4158 4243 5314 2235 Size: 1143 780 85 1071 3018 Fragments arranged by size: 3018 1143 1071 780 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 BsmBI CGTCTCn'nnnn_ Cuts at: 2825 2825 Size: 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2235 2298 2743 3378 3416 4158 4243 Size: 399 445 63 445 635 38 742 85 Cuts at: 4243 5314 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 635 445 445 399 85 63 38 BspEI T'CCGG_A Cuts at: 3315 5760 3315 Size: 2445 3652 BspHI T'CATG_A Cuts at: 2706 3986 5716 2706 Size: 1280 1730 3087 Fragments arranged by size: 3087 1730 1280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2697 3135 3333 3452 844 Size: 1853 438 198 119 3489 Fragments arranged by size: 3489 1853 438 198 119 BsrBI CCG'CTC Cuts at: 1394 1823 2745 2773 2791 2830 3984 1394 Size: 429 922 28 18 39 1154 3507 Fragments arranged by size: 3507 1154 922 429 39 28 18 BsrDI GCAATG_nn' Cuts at: 65 1248 2415 4592 4766 65 Size: 1183 1167 2177 174 1396 Fragments arranged by size: 2177 1396 1183 1167 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2697 4741 4928 1700 Size: 110 887 2044 187 2869 Fragments arranged by size: 2869 2044 887 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 2854 4157 5169 2854 Size: 1303 1012 3782 Fragments arranged by size: 3782 1303 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3373 794 Size: 2579 3518 BstBI TT'CG_AA Cuts at: 4901 4901 Size: 6097 BstEII G'GTnAC_C Cuts at: 1184 2191 1184 Size: 1007 5090 BstXI CCAn_nnnn'nTGG Cuts at: 2890 3121 3139 2890 Size: 231 18 5848 Fragments arranged by size: 5848 231 18 BstYI r'GATC_y Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092 Size: 1455 367 385 17 1452 86 12 346 Cuts at: 6092 1972 Size: 1977 Fragments arranged by size: 1977 1455 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2754 2754 Size: 6097 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5358 Fragments arranged by size: 5358 683 56 BtsI GCAGTG_nn' Cuts at: 3613 4407 4427 3613 Size: 794 20 5283 Fragments arranged by size: 5283 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 2671 3401 2671 Size: 730 5367 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3092 5104 809 Size: 507 1776 2012 1802 Fragments arranged by size: 2012 1802 1776 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2172 2535 2831 Size: 54 1425 50 275 18 342 363 296 Cuts at: 2831 3544 4433 6062 8 Size: 713 889 1629 43 Fragments arranged by size: 1629 1425 889 713 363 342 296 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2708 4027 1353 Size: 1355 1319 3423 Fragments arranged by size: 3423 1355 1319 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2630 3098 4684 5058 5204 387 Size: 1064 138 1041 468 1586 374 146 1280 Fragments arranged by size: 1586 1280 1064 1041 468 374 146 138 EcoICRI GAG'CTC Cuts at: 2233 2233 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2315 2375 3068 3097 4160 4757 Size: 393 629 54 60 693 29 1063 597 Cuts at: 4757 5544 5812 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1063 787 693 629 597 393 268 60 54 29 EcoO109I rG'GnC_Cy Cuts at: 2295 2739 2758 2972 2295 Size: 444 19 214 5420 Fragments arranged by size: 5420 444 214 19 EcoRI G'AATT_C Cuts at: 2250 3406 3784 2250 Size: 1156 378 4563 Fragments arranged by size: 4563 1156 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2345 2345 Size: 6097 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2036 2080 2086 2566 5244 1678 Size: 358 44 6 480 2678 2531 Fragments arranged by size: 2678 2531 480 358 44 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4738 4770 4812 4844 1373 Size: 32 135 32 3166 32 42 32 2626 Fragments arranged by size: 3166 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2268 3441 3687 669 Size: 988 281 330 1173 246 3079 Fragments arranged by size: 3079 1173 988 330 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KasI G'GCGC_C Cuts at: 2082 2562 2082 Size: 480 5617 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 2816 5973 2816 Size: 3157 2940 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5211 5395 1305 Size: 3906 184 2007 Fragments arranged by size: 3906 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 2537 10 Size: 54 1425 343 342 363 3570 Fragments arranged by size: 3570 1425 363 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2478 3137 4055 4414 4573 518 Size: 648 1312 659 918 359 159 2042 Fragments arranged by size: 2042 1312 918 659 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 3262 3513 4190 5338 5583 1606 Size: 447 1209 251 677 1148 245 2120 Fragments arranged by size: 2120 1209 1148 677 447 251 245 NarI GG'CG_CC Cuts at: 2083 2563 2083 Size: 480 5617 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4606 Fragments arranged by size: 4606 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2108 2725 3151 3455 5000 1132 Size: 97 879 617 426 304 1545 2229 Fragments arranged by size: 2229 1545 879 617 426 304 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 3147 4996 3147 Size: 1849 4248 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2758 2758 Size: 6097 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2294 2739 2294 Size: 445 5652 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2537 2569 2537 Size: 32 6065 PstI C_TGCA'G Cuts at: 830 2694 3267 3449 830 Size: 1864 573 182 3478 Fragments arranged by size: 3478 1864 573 182 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 3262 3262 Size: 6097 SacI G_AGCT'C Cuts at: 2235 2235 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SapI GCTCTTCn'nnn_ Cuts at: 2708 2708 Size: 6097 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 2690 3263 3445 4580 5261 5452 826 Size: 219 1645 573 182 1135 681 191 1471 Fragments arranged by size: 1645 1471 1135 681 573 219 191 182 SfoI GGC'GCC Cuts at: 2084 2564 2084 Size: 480 5617 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 3424 1872 Size: 1552 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3888 493 Size: 3395 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 2725 3455 1132 Size: 1593 730 3774 Fragments arranged by size: 3774 1593 730 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2004 3536 513 Size: 552 939 1532 3074 Fragments arranged by size: 3074 1532 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3080 3352 4124 4141 4294 4479 4924 Size: 1255 1500 272 772 17 153 185 445 Cuts at: 4924 325 Size: 1498 Fragments arranged by size: 1500 1498 1255 772 445 272 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2326 2340 4343 Size: 274 80 33 51 1443 349 14 2003 Cuts at: 4343 6055 96 Size: 1712 138 Fragments arranged by size: 2003 1712 1443 349 274 138 80 51 33 14 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3095 3127 6057 6089 3095 Size: 32 2930 32 3103 Fragments arranged by size: 3103 2930 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2762 1316 Size: 1446 4651 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 3139 2497 Size: 642 5455 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 3422 1870 Size: 1552 4545 XmnI GAAnn'nnTTC Cuts at: 3014 4226 3014 Size: 1212 4885 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI