################################## # NO CUT ENZYMES ( 98 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII BbeI BbvCI BcgI BciVI BfrI BglII BsaBI BsaWI BseAI BsiWI BsmBI BsmI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV FspI HincII HindII HindIII HpaI KasI KpnI MamI MfeI MluI MroI MseI MunI NarI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SfiI SfoI SfuI SmlI SnaBI SpeI SphI SspI StuI SwaI TaqI TfiI Tru9I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AcsI 1096 AAATTC [GA]^AATT[CT] AocI 924 CCTCAGG CC^T[ACGT]AGG ApoI 1096 AAATTC [GA]^AATT[CT] AspI 268 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1303 CCTAGG C^CTAGG BalI 1496 TGGCCA TGG^CCA BamHI 779 GGATCC G^GATCC BbrPI 123 CACGTG CAC^GTG BbsI 1453 GTCTTC GAAGAC(2/6) BclI 656 TGATCA T^GATCA BfaI 1304 CTAG C^TAG BglI 1483 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlnI 1303 CCTAGG C^CTAGG BpuAI 1453 GTCTTC GAAGAC(2/6) BsaAI 123 CACGTG [CT]AC^GT[GA] BsiEI 1353 CGACCG CG[GA][CT]^CG Bsp1407I 1130 TGTACA T^GTACA BsrDI 1134 CATTGC GCAATG(2/0) BsrGI 1130 TGTACA T^GTACA BstEII 363 GGTGACC G^GT[ACGT]ACC BstPI 363 GGTGACC G^GT[ACGT]ACC BstXI 598 CCAGGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 924 CCTCAGG CC^T[ACGT]AGG DraIII 1335 CACCGCGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 1354 GACCGGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 924 CCTCAGG CC^T[ACGT]AGG EcoO65I 363 GGTGACC G^GT[ACGT]ACC EcoT22I 1346 ATGCAT ATGCA^T FbaI 656 TGATCA T^GATCA FseI 969 GGCCGGCC GGCCGG^CC KspI 62 CCGCGG CCGC^GG MaeI 1304 CTAG C^TAG MaeII 124 ACGT A^CGT MluNI 1496 TGGCCA TGG^CCA MscI 1496 TGGCCA TGG^CCA MslI 1998 CATGGCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NcoI 474 CCATGG C^CATGG NsiI 1346 ATGCAT ATGCA^T PflFI 268 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 123 CACGTG CAC^GTG PmlI 123 CACGTG CAC^GTG PshAI 646 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacII 62 CCGCGG CCGC^GG SbfI 1855 CCTGCAGG CCTGCA^GG SexAI 269 ACCAGGT A^CC[AT]GGT SgrAI 23 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 1855 CCTGCAGG CCTGCA^GG SspBI 1130 TGTACA T^GTACA Tsp509I 1097 AATT ^AATT Tth111I 268 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 90 GTCTAC GT^[AC][GT]AC 460 GTCTAC AcyI 21 GACGCC G[GA]^CG[CT]C 46 GACGCC AfaI 312 GTAC GT^AC 1131 GTAC ApaI 1184 GGGCCC GGGCC^C 1650 GGGCCC BlpI 672 GCTCAGC GC^T[ACGT]AGC 1800 GCTGAGC Bpu1102I 672 GCTCAGC GC^T[ACGT]AGC 1800 GCTGAGC BsaHI 21 GACGCC G[GA]^CG[CT]C 46 GACGCC BsaI 1110 GGTCTC GGTCTC(1/5) 1989 GGTCTC BstAPI 198 GCAGGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1334 GCACCGCGTGC BstYI 779 GGATCC [GA]^GATC[CT] 1150 AGATCC CelII 672 GCTCAGC GC^T[ACGT]AGC 1800 GCTGAGC EarI 76 CTCTTC CTCTTC(1/4) 249 GAAGAG HaeII 1020 GGCGCT [GA]GCGC^[CT] 1480 AGCGCC Hin1I 21 GACGCC G[GA]^CG[CT]C 46 GACGCC Ksp632I 76 CTCTTC CTCTTC(1/4) 249 GAAGAG MflI 779 GGATCC [GA]^GATC[CT] 1150 AGATCC PvuII 628 CAGCTG CAG^CTG 660 CAGCTG RsaI 312 GTAC GT^AC 1131 GTAC SapI 249 GAAGAGC GCTCTTC(1/4) 75 GCTCTTC XcmI 1316 CCATGCCCCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1484 CCATCAGGGCAGTGG XhoII 779 GGATCC [GA]^GATC[CT] 1150 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AccII AciI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PleI PpuMI PstI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI StyI TseI Tsp45I TspRI Van91I XmaCI XmaI